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The map label for this gene is IS1086 [H]

Identifier: 183219474

GI number: 183219474

Start: 45843

End: 46835

Strand: Reverse

Name: IS1086 [H]

Synonym: LEPBI_I0047

Alternate gene names: 183219474

Gene position: 46835-45843 (Counterclockwise)

Preceding gene: 183219475

Following gene: 183219472

Centisome position: 1.3

GC content: 34.94

Gene sequence:

>993_bases
ATGGAAAAATACTCTAGAATTAGTCCGAAGGAAAGGGAAACCTTGTCCGTGTTGATTCATCAAGGATACTCTAAAGGTGC
GATTGCAGAAATGATGGGTCGTCACCGATCTACAATTAGTAGAGAATTAGAAAAAGGAAAAATTTCCGATAGATATTATC
AATACTCCGCAATTATTTCTGGGTATAAATCAAGAATGGCTGCCTCATCTAGAAAGCTTGGAAAAAGTAAATTATTAACT
AACAACAAGTTGTATAGGGAGTTTAAAAATCGTCTTGAACTCAAATGGTCACCAGTACAAATATCAAAAAATCTATCTCG
TTCGTTTCCAAATGAAAAATATATGAATATTTCTCACCAATCAATTTATACCTTTATCTATTTGCTTCCGAGAGGAGAGC
TTAAAAAAGAAATCAATACTTACCTTAGAAGAAAAGGCAAAAAAGCTAAACCTGCGGGAATCAAGGCCAATATAGGTAAA
ATTCCAGATATGATCAGCATTGAAGAACGACCAAAGGAAGTCGCTGATAGGTCTGTTCCAGGTCATTGGGAAGGCGATTT
ACTCATAGGAAATAACCATTCAAGTGCTCTTGGTACAATTGTAGAAAGAGTTACAAGAACTGTCATACTAGTACCTTTGA
AAGCCATGGATGCTGAATCTGTCAGAAAGGCCTTTGAACAAGAGTTAAAACAGTTTCCAAAACAATTAAAACTTTCTATG
ACTTATGACCAAGGAAAAGAAATGTCAGAACATAGATTATTTACTAAAAATACAAAAATGAAAGTTTATTTCTGTCATCC
AGCTTCTCCATGGGAAAGGGGAACATGTGAAAATACTAATATGCTTATCAGAGACTTCTTTCCAAAGGGAACTGACTTTT
CAAAACTCAAAAAAAGCCAAATTAAAAAGGTTCAAACTTTACTCAATCAAAGACCTAGAAAAACTTTGAATTGGAATACA
CCTGAAGAAGAAATACGGGCGCTGGTGCGCTAG

Upstream 100 bases:

>100_bases
ACCTAGAGATAAGTCGCTATTATGCGATGTTTTCGGGATTAAAAAAGGTAGGTTCAAGAATCTAATGCAACAAAACCCGA
TAATGTAAATCGGAGAAAAA

Downstream 100 bases:

>100_bases
ATTCTTGAACCTACCAAAGTAATTACGCCACGGACGTCGTTCTTTATAGCAACCTGACTTTGAAGTTTTGATTTTATATT
CTTTAACAAGAATGTCGGTA

Product: IS30 family transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MEKYSRISPKERETLSVLIHQGYSKGAIAEMMGRHRSTISRELEKGKISDRYYQYSAIISGYKSRMAASSRKLGKSKLLT
NNKLYREFKNRLELKWSPVQISKNLSRSFPNEKYMNISHQSIYTFIYLLPRGELKKEINTYLRRKGKKAKPAGIKANIGK
IPDMISIEERPKEVADRSVPGHWEGDLLIGNNHSSALGTIVERVTRTVILVPLKAMDAESVRKAFEQELKQFPKQLKLSM
TYDQGKEMSEHRLFTKNTKMKVYFCHPASPWERGTCENTNMLIRDFFPKGTDFSKLKKSQIKKVQTLLNQRPRKTLNWNT
PEEEIRALVR

Sequences:

>Translated_330_residues
MEKYSRISPKERETLSVLIHQGYSKGAIAEMMGRHRSTISRELEKGKISDRYYQYSAIISGYKSRMAASSRKLGKSKLLT
NNKLYREFKNRLELKWSPVQISKNLSRSFPNEKYMNISHQSIYTFIYLLPRGELKKEINTYLRRKGKKAKPAGIKANIGK
IPDMISIEERPKEVADRSVPGHWEGDLLIGNNHSSALGTIVERVTRTVILVPLKAMDAESVRKAFEQELKQFPKQLKLSM
TYDQGKEMSEHRLFTKNTKMKVYFCHPASPWERGTCENTNMLIRDFFPKGTDFSKLKKSQIKKVQTLLNQRPRKTLNWNT
PEEEIRALVR
>Mature_330_residues
MEKYSRISPKERETLSVLIHQGYSKGAIAEMMGRHRSTISRELEKGKISDRYYQYSAIISGYKSRMAASSRKLGKSKLLT
NNKLYREFKNRLELKWSPVQISKNLSRSFPNEKYMNISHQSIYTFIYLLPRGELKKEINTYLRRKGKKAKPAGIKANIGK
IPDMISIEERPKEVADRSVPGHWEGDLLIGNNHSSALGTIVERVTRTVILVPLKAMDAESVRKAFEQELKQFPKQLKLSM
TYDQGKEMSEHRLFTKNTKMKVYFCHPASPWERGTCENTNMLIRDFFPKGTDFSKLKKSQIKKVQTLLNQRPRKTLNWNT
PEEEIRALVR

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786450, Length=327, Percent_Identity=37.9204892966361, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1790736, Length=327, Percent_Identity=37.6146788990826, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1787671, Length=327, Percent_Identity=37.6146788990826, Blast_Score=193, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 38349; Mature: 38349

Theoretical pI: Translated: 10.70; Mature: 10.70

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKYSRISPKERETLSVLIHQGYSKGAIAEMMGRHRSTISRELEKGKISDRYYQYSAIIS
CCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GYKSRMAASSRKLGKSKLLTNNKLYREFKNRLELKWSPVQISKNLSRSFPNEKYMNISHQ
HHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHCCCHHH
SIYTFIYLLPRGELKKEINTYLRRKGKKAKPAGIKANIGKIPDMISIEERPKEVADRSVP
HHHHHHEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCC
GHWEGDLLIGNNHSSALGTIVERVTRTVILVPLKAMDAESVRKAFEQELKQFPKQLKLSM
CCCCCCEEEECCCCHHHHHHHHHHHHHEEEEEHHHCCHHHHHHHHHHHHHHHHHHHEEEC
TYDQGKEMSEHRLFTKNTKMKVYFCHPASPWERGTCENTNMLIRDFFPKGTDFSKLKKSQ
CHHCCCCHHHHHHEECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHH
IKKVQTLLNQRPRKTLNWNTPEEEIRALVR
HHHHHHHHHCCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MEKYSRISPKERETLSVLIHQGYSKGAIAEMMGRHRSTISRELEKGKISDRYYQYSAIIS
CCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GYKSRMAASSRKLGKSKLLTNNKLYREFKNRLELKWSPVQISKNLSRSFPNEKYMNISHQ
HHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHCCCHHH
SIYTFIYLLPRGELKKEINTYLRRKGKKAKPAGIKANIGKIPDMISIEERPKEVADRSVP
HHHHHHEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCC
GHWEGDLLIGNNHSSALGTIVERVTRTVILVPLKAMDAESVRKAFEQELKQFPKQLKLSM
CCCCCCEEEECCCCHHHHHHHHHHHHHEEEEEHHHCCHHHHHHHHHHHHHHHHHHHEEEC
TYDQGKEMSEHRLFTKNTKMKVYFCHPASPWERGTCENTNMLIRDFFPKGTDFSKLKKSQ
CHHCCCCHHHHHHEECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHH
IKKVQTLLNQRPRKTLNWNTPEEEIRALVR
HHHHHHHHHCCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1334071 [H]