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The map label for this gene is gyrB1 [H]

Identifier: 183219432

GI number: 183219432

Start: 4333

End: 6252

Strand: Direct

Name: gyrB1 [H]

Synonym: LEPBI_I0005

Alternate gene names: 183219432

Gene position: 4333-6252 (Clockwise)

Preceding gene: 183219431

Following gene: 183219433

Centisome position: 0.12

GC content: 40.57

Gene sequence:

>1920_bases
ATGTCCAACCAAACCGATCAAAACGCCTATTCAGCCTCAAAAATCAAGATCCTAGAGGGTCTAGAGGCGGTCCGGAAACG
TCCCGGAATGTATATCGGAACCCAAGATGAATCCGGCCTTCATAAGATGGTATATGAGGTCGTAGACAATTCAGTGGATG
AGGCAATGGCCGGCCATTGTACAGAAATTGATGTTCGTATCCTACCTGAAAATATCATCGAAGTTCGCGATAATGGTCGA
GGGATTCCGACCGGTATCCACCCAGATAAGGGAAAGTCAACCATCGAAGTGGTTCTTACCATCTTACACGCAGGTGGAAA
ATTTGAAAATGATGCTTATAAGGTTTCAGGTGGTTTGCATGGGGTTGGGGTATCCGTTGTGAACGCACTTTCTACATACT
TAGAAGTAGAGGTGCACCAAGAAGGAAAACTCCATTACCAAAAATACCAAGCGGGAGTCCCTGTTGAAGATGTAAAAATC
ATAGGGGAGACTACTCACCGCGGAACTGTCGTTCGGTTTCGACCAGACCATACCATTTTTACGACGGTAGATTTTTCTTT
CGATACACTTTCCGCAAGGTTTCGAGAAATTGCTTTCCTTAACAAAGGTCTTCTCATTCGAATCGAAGACCAAAGAAAGG
AAGAATTCGCCAAACATGAGTTTAAGTTCGATGGCGGAATTGTTTCCTTTGTGGAATACATCACTGAATCCAAACACCCA
CTTCATAAAGTATTACACTTTGTAGGAGAAAAAGAAAATGTTTGGGCCGAAATTGCTCTCCAATATTGTGATACCTATAG
TGAAAATATTTTCTGTTTTACAAACGCAATTAATAACAACTTAGGTGGAACACACTTAGAAGGTTTTAGAACAGCATTGA
CTCGAACTCTCAATGACCATCTAAAAAAAGACCAAACTTTATTCAAAAAACAACCCAATGGATTACAAGGGGATGATATC
AAAGAAGGTCTTTGTGCCGTCATTTCCATCAAAATCCCACAACCGCAATTCAACTCTCAAACAAAAGAAAAATTAGTGAA
TGCGGAAGTAAAGGGTCTCATGCAAACCATCACGGGAGAAGGACTCAATCGTTACTTTGAAGAAAACCCTGCGATCATCA
AAAAGATTCTCGAAAAATGTATCTTAGCATCAAAAGCAAGAGAAGCGGCAAGGCGTGCTCGTGATCTTACAAGACGTAAA
ACCGTTTTAGAAGGTGGTGGGCTTCCTGGTAAACTTGCCGATTGTTCAGAAAAAGATCCTGAGCATTGCGAACTCTTTTT
AGTCGAGGGGGATTCAGCGGGTGGATCAGCCAAACAAGGAAGAGATAGAAATACGCAAGCCATCCTCCCACTCAAAGGTA
AAATCTTAAACGTAGAAAAAGCAAGGTTAGATAAAATCCTCTCCAATGAAGAGATTCGCACATTAATCACTGTTATGGGA
ACTGGCATTGGTGATGATGAATTTAATGTGGAAAAACTTCGTTACAGAAAAATCATCATCATGACAGATGCGGACGTGGA
TGGATCTCATATTCGAACTCTGCTCTTAACATTTTTCTTTCGATACATGAAACCTGTGATTGAACGTGGTTCCCTCTATG
TTGCACAACCTCCATTGTATTTACTTAAATTTGGTAAGGAAGCAGTGTATGTTTACTCTGATCGAGAGAAAGATGAAATC
TTAAAAGCTAGACCAAATGACAAAGTGGTCATCCAACGATATAAGGGACTTGGAGAGATGAACCCGGAACAACTTTGGGA
TACTACTATGGATCCAAAAGAACGTGTCATGTTACAAGTGAAATTACAAGATTTCGTAGAGGCAGAAGATACATTCAATA
TCCTAATGGGTGATGAAGTGTCTCCTCGTCGTCGTTTCATTGAAGCGAATTCTTACAAAGTAGCAAATTTAGATCTTTAA

Upstream 100 bases:

>100_bases
ATTCCAAAATCCTAGGTCTTAAACCCTCACATTCCCCCCGATTTCACTTGCCCTTCGAGGGTCTTTTTAGGATACTTCCT
ATAGGGTATCTTAAAAATCT

Downstream 100 bases:

>100_bases
TCGGAATTAGGAAAATCAAATGACCGAACAAAACGGCCAAGAAAACGAATCAAACAAAACCTTAGCACTCAATTTATCCG
GTAGACCAGACGTAGCCGGT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 639; Mature: 638

Protein sequence:

>639_residues
MSNQTDQNAYSASKIKILEGLEAVRKRPGMYIGTQDESGLHKMVYEVVDNSVDEAMAGHCTEIDVRILPENIIEVRDNGR
GIPTGIHPDKGKSTIEVVLTILHAGGKFENDAYKVSGGLHGVGVSVVNALSTYLEVEVHQEGKLHYQKYQAGVPVEDVKI
IGETTHRGTVVRFRPDHTIFTTVDFSFDTLSARFREIAFLNKGLLIRIEDQRKEEFAKHEFKFDGGIVSFVEYITESKHP
LHKVLHFVGEKENVWAEIALQYCDTYSENIFCFTNAINNNLGGTHLEGFRTALTRTLNDHLKKDQTLFKKQPNGLQGDDI
KEGLCAVISIKIPQPQFNSQTKEKLVNAEVKGLMQTITGEGLNRYFEENPAIIKKILEKCILASKAREAARRARDLTRRK
TVLEGGGLPGKLADCSEKDPEHCELFLVEGDSAGGSAKQGRDRNTQAILPLKGKILNVEKARLDKILSNEEIRTLITVMG
TGIGDDEFNVEKLRYRKIIIMTDADVDGSHIRTLLLTFFFRYMKPVIERGSLYVAQPPLYLLKFGKEAVYVYSDREKDEI
LKARPNDKVVIQRYKGLGEMNPEQLWDTTMDPKERVMLQVKLQDFVEAEDTFNILMGDEVSPRRRFIEANSYKVANLDL

Sequences:

>Translated_639_residues
MSNQTDQNAYSASKIKILEGLEAVRKRPGMYIGTQDESGLHKMVYEVVDNSVDEAMAGHCTEIDVRILPENIIEVRDNGR
GIPTGIHPDKGKSTIEVVLTILHAGGKFENDAYKVSGGLHGVGVSVVNALSTYLEVEVHQEGKLHYQKYQAGVPVEDVKI
IGETTHRGTVVRFRPDHTIFTTVDFSFDTLSARFREIAFLNKGLLIRIEDQRKEEFAKHEFKFDGGIVSFVEYITESKHP
LHKVLHFVGEKENVWAEIALQYCDTYSENIFCFTNAINNNLGGTHLEGFRTALTRTLNDHLKKDQTLFKKQPNGLQGDDI
KEGLCAVISIKIPQPQFNSQTKEKLVNAEVKGLMQTITGEGLNRYFEENPAIIKKILEKCILASKAREAARRARDLTRRK
TVLEGGGLPGKLADCSEKDPEHCELFLVEGDSAGGSAKQGRDRNTQAILPLKGKILNVEKARLDKILSNEEIRTLITVMG
TGIGDDEFNVEKLRYRKIIIMTDADVDGSHIRTLLLTFFFRYMKPVIERGSLYVAQPPLYLLKFGKEAVYVYSDREKDEI
LKARPNDKVVIQRYKGLGEMNPEQLWDTTMDPKERVMLQVKLQDFVEAEDTFNILMGDEVSPRRRFIEANSYKVANLDL
>Mature_638_residues
SNQTDQNAYSASKIKILEGLEAVRKRPGMYIGTQDESGLHKMVYEVVDNSVDEAMAGHCTEIDVRILPENIIEVRDNGRG
IPTGIHPDKGKSTIEVVLTILHAGGKFENDAYKVSGGLHGVGVSVVNALSTYLEVEVHQEGKLHYQKYQAGVPVEDVKII
GETTHRGTVVRFRPDHTIFTTVDFSFDTLSARFREIAFLNKGLLIRIEDQRKEEFAKHEFKFDGGIVSFVEYITESKHPL
HKVLHFVGEKENVWAEIALQYCDTYSENIFCFTNAINNNLGGTHLEGFRTALTRTLNDHLKKDQTLFKKQPNGLQGDDIK
EGLCAVISIKIPQPQFNSQTKEKLVNAEVKGLMQTITGEGLNRYFEENPAIIKKILEKCILASKAREAARRARDLTRRKT
VLEGGGLPGKLADCSEKDPEHCELFLVEGDSAGGSAKQGRDRNTQAILPLKGKILNVEKARLDKILSNEEIRTLITVMGT
GIGDDEFNVEKLRYRKIIIMTDADVDGSHIRTLLLTFFFRYMKPVIERGSLYVAQPPLYLLKFGKEAVYVYSDREKDEIL
KARPNDKVVIQRYKGLGEMNPEQLWDTTMDPKERVMLQVKLQDFVEAEDTFNILMGDEVSPRRRFIEANSYKVANLDL

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=608, Percent_Identity=27.3026315789474, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI19913406, Length=608, Percent_Identity=26.3157894736842, Blast_Score=142, Evalue=7e-34,
Organism=Escherichia coli, GI48994957, Length=555, Percent_Identity=57.6576576576577, Blast_Score=628, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=613, Percent_Identity=39.1517128874388, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17535065, Length=575, Percent_Identity=25.5652173913043, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI212645845, Length=535, Percent_Identity=26.1682242990654, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI212645657, Length=188, Percent_Identity=32.4468085106383, Blast_Score=84, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324241, Length=565, Percent_Identity=28.8495575221239, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17136538, Length=565, Percent_Identity=27.2566371681416, Blast_Score=139, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72139; Mature: 72008

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQTDQNAYSASKIKILEGLEAVRKRPGMYIGTQDESGLHKMVYEVVDNSVDEAMAGHC
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCHHHHHCCCC
TEIDVRILPENIIEVRDNGRGIPTGIHPDKGKSTIEVVLTILHAGGKFENDAYKVSGGLH
CEEEEEECCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCC
GVGVSVVNALSTYLEVEVHQEGKLHYQKYQAGVPVEDVKIIGETTHRGTVVRFRPDHTIF
HHHHHHHHHHHHHHEEEECCCCCEEHHHHHCCCCHHHEEEEECCCCCCEEEEECCCCEEE
TTVDFSFDTLSARFREIAFLNKGLLIRIEDQRKEEFAKHEFKFDGGIVSFVEYITESKHP
EEEECCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCH
LHKVLHFVGEKENVWAEIALQYCDTYSENIFCFTNAINNNLGGTHLEGFRTALTRTLNDH
HHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
LKKDQTLFKKQPNGLQGDDIKEGLCAVISIKIPQPQFNSQTKEKLVNAEVKGLMQTITGE
HHHHHHHHHCCCCCCCCCCHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GLNRYFEENPAIIKKILEKCILASKAREAARRARDLTRRKTVLEGGGLPGKLADCSEKDP
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
EHCELFLVEGDSAGGSAKQGRDRNTQAILPLKGKILNVEKARLDKILSNEEIRTLITVMG
CCEEEEEEECCCCCCCCCCCCCCCCEEEEECCCEEECHHHHHHHHHHCCHHHHHHHHHHC
TGIGDDEFNVEKLRYRKIIIMTDADVDGSHIRTLLLTFFFRYMKPVIERGSLYVAQPPLY
CCCCCCCCCHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHH
LLKFGKEAVYVYSDREKDEILKARPNDKVVIQRYKGLGEMNPEQLWDTTMDPKERVMLQV
EECCCCCEEEEECCCCHHHHHHCCCCCCEEHEEHHCCCCCCHHHHHCCCCCHHHHEEEEE
KLQDFVEAEDTFNILMGDEVSPRRRFIEANSYKVANLDL
EHHHHHCCCCCEEEEECCCCCHHHHEEECCCEEEEECCC
>Mature Secondary Structure 
SNQTDQNAYSASKIKILEGLEAVRKRPGMYIGTQDESGLHKMVYEVVDNSVDEAMAGHC
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCHHHHHCCCC
TEIDVRILPENIIEVRDNGRGIPTGIHPDKGKSTIEVVLTILHAGGKFENDAYKVSGGLH
CEEEEEECCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCC
GVGVSVVNALSTYLEVEVHQEGKLHYQKYQAGVPVEDVKIIGETTHRGTVVRFRPDHTIF
HHHHHHHHHHHHHHEEEECCCCCEEHHHHHCCCCHHHEEEEECCCCCCEEEEECCCCEEE
TTVDFSFDTLSARFREIAFLNKGLLIRIEDQRKEEFAKHEFKFDGGIVSFVEYITESKHP
EEEECCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCH
LHKVLHFVGEKENVWAEIALQYCDTYSENIFCFTNAINNNLGGTHLEGFRTALTRTLNDH
HHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
LKKDQTLFKKQPNGLQGDDIKEGLCAVISIKIPQPQFNSQTKEKLVNAEVKGLMQTITGE
HHHHHHHHHCCCCCCCCCCHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GLNRYFEENPAIIKKILEKCILASKAREAARRARDLTRRKTVLEGGGLPGKLADCSEKDP
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
EHCELFLVEGDSAGGSAKQGRDRNTQAILPLKGKILNVEKARLDKILSNEEIRTLITVMG
CCEEEEEEECCCCCCCCCCCCCCCCEEEEECCCEEECHHHHHHHHHHCCHHHHHHHHHHC
TGIGDDEFNVEKLRYRKIIIMTDADVDGSHIRTLLLTFFFRYMKPVIERGSLYVAQPPLY
CCCCCCCCCHHEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHH
LLKFGKEAVYVYSDREKDEILKARPNDKVVIQRYKGLGEMNPEQLWDTTMDPKERVMLQV
EECCCCCEEEEECCCCHHHHHHCCCCCCEEHEEHHCCCCCCHHHHHCCCCCHHHHEEEEE
KLQDFVEAEDTFNILMGDEVSPRRRFIEANSYKVANLDL
EHHHHHCCCCCEEEEECCCCCHHHHEEECCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377 [H]