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The map label for this gene is leuA [H]

Identifier: 182677591

GI number: 182677591

Start: 665986

End: 667584

Strand: Direct

Name: leuA [H]

Synonym: Bind_0596

Alternate gene names: 182677591

Gene position: 665986-667584 (Clockwise)

Preceding gene: 182677588

Following gene: 182677592

Centisome position: 15.97

GC content: 57.79

Gene sequence:

>1599_bases
ATGACCACGAATAAGTCTTCCGAACCGGCAATGGGGTCCGCCATCCAGGAAACCGCCAATACGGAACAAATCATCATCTT
CGACACGACTCTTCGCGATGGCGAACAATCGCCCGGTGCCTCGATGAATCTCGACGAGAAGATCGAAGTAGCCGAACTCT
TGGACGCGATGGGCGTCGATGTGATCGAGGCGGGTTTTCCCGTCACGTCGGAGGGGGATTTCGAGGCCGTTTCGGCCATC
TCCCGCCGCGTCAAGCGGGCGAGTGTCGCCGGTCTTGCCCGCGCTGCAGCGCGCGATATCGATCGTTGCGCCGAGGCCCT
GCGTGATGCCGTACGGCCCCGCATGCATGTCTTCATCTCGACCTCTCCGCTGCATATGAAATATAAATTGCAGAAGGAGC
CGGGCGAAGTCCTTGACATGATTTCGACCTATGTCGGACGGGCGCGCAATCGTATCGATGATATCGAATGGTCGGCTGAG
GACGGCACCCGCACGGAGATCGATTTTCTGTGCCGCTGCGTGGAAGCGGCGATCAAGGCCGGCGCACGCACGATCAATAT
TCCTGACACGGTCGGTTATACGATTCCGGAAGAATATCAGGCTTTGTTCGAAACAGTGCGGACGCGCGTTCCTAATTCCG
ACCAGGCGATCTTCTCGGTCCATTGTCACAACGATCTCGGCCTTGCCGTGGCCAATACTTTGGCCGGTATCAAGGGTGGC
GCGCGGCAGGTGGAATGCACGATCAATGGTCTTGGCGAGCGAGCTGGCAATGCCGCCATGGAAGAGGTCGTCATGGCCTT
GCGGGTGCGGGCAGACGCCTTGGCCTATCACAGCAATATCGATGCACGCATGTTGACGCGAGCCTCGAAGCTCGTTTCGG
CAGTCAGCTCCTTCCCGGTGCAATATAACAAGGCGATCGTTGGTCGGAACGCCTTCGCTCACGAGAGCGGTATTCATCAG
GATGGCATGCTGAAAAATGCGCAGACCTATGAAATCATGACGCCGGAAAGTGTCGGCGTGCATAAAACCTCACTGGTCAT
GGGCAAGCATTCGGGCCGCCACGCCTTCAAGGAAAAATTGAAGGAACTTGGTTATGAGCTCGGTGAAAACGCGCTCGAGG
AAGCTTTCGCTCGATTCAAGGATCTCGCTGACCGCAAGAAGATCGTCTACGATGAAGATTTGATCGCTTTGGTGGATGAT
GAAATTATTCACGCGCATGATCGAATCAAGCTTGTGGCCCTCACCGTGATCGCCGGAACCAAGGGTCCGCAATCGGCGGC
CTTAACGCTGACGATCGACGGGGTGGAGCAAACCCATCAGGCGACGGGGAATGGCCCAATCGACGCTATTTTCAATGCCG
TGCAGGCCTTGGTGCCGCATGGGGCCGTGCTGGAACTTTATCAGGTCCATGCCGTCACCAAGGGCACAGATGCGCAGGCG
GAAGTCTCAGTGCGCTTGAGCGAGGATGGCAAGACCGTGACGGCCCGTGGCGCCGATCCGGATACGCTGGTCGCCTCGGC
CAAGGCCTATATTGCCGCCTTGAATAAACTGGTCGTGCGGCGACACAAGAGCCGCCCCGACGACAGCCTCGCCGGTTGA

Upstream 100 bases:

>100_bases
TTGCTGCTTCTTAGTTGCCCCTGAGAGCCGGCCGGGCCTTGCCTGGGCACTCAGGGGATCGATCGAAGTTTCAGCATCTC
TCCTCACCCAGGACAAAATC

Downstream 100 bases:

>100_bases
GATTGTGCGATAAGGTCTTGAAACCTTCAAGAAGGGCTAGACAGGTGAGCCCGCGTTTGCTAAATGCGCGTTCACCTGTT
TCGGACACCCACGGTGTTTA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MTTNKSSEPAMGSAIQETANTEQIIIFDTTLRDGEQSPGASMNLDEKIEVAELLDAMGVDVIEAGFPVTSEGDFEAVSAI
SRRVKRASVAGLARAAARDIDRCAEALRDAVRPRMHVFISTSPLHMKYKLQKEPGEVLDMISTYVGRARNRIDDIEWSAE
DGTRTEIDFLCRCVEAAIKAGARTINIPDTVGYTIPEEYQALFETVRTRVPNSDQAIFSVHCHNDLGLAVANTLAGIKGG
ARQVECTINGLGERAGNAAMEEVVMALRVRADALAYHSNIDARMLTRASKLVSAVSSFPVQYNKAIVGRNAFAHESGIHQ
DGMLKNAQTYEIMTPESVGVHKTSLVMGKHSGRHAFKEKLKELGYELGENALEEAFARFKDLADRKKIVYDEDLIALVDD
EIIHAHDRIKLVALTVIAGTKGPQSAALTLTIDGVEQTHQATGNGPIDAIFNAVQALVPHGAVLELYQVHAVTKGTDAQA
EVSVRLSEDGKTVTARGADPDTLVASAKAYIAALNKLVVRRHKSRPDDSLAG

Sequences:

>Translated_532_residues
MTTNKSSEPAMGSAIQETANTEQIIIFDTTLRDGEQSPGASMNLDEKIEVAELLDAMGVDVIEAGFPVTSEGDFEAVSAI
SRRVKRASVAGLARAAARDIDRCAEALRDAVRPRMHVFISTSPLHMKYKLQKEPGEVLDMISTYVGRARNRIDDIEWSAE
DGTRTEIDFLCRCVEAAIKAGARTINIPDTVGYTIPEEYQALFETVRTRVPNSDQAIFSVHCHNDLGLAVANTLAGIKGG
ARQVECTINGLGERAGNAAMEEVVMALRVRADALAYHSNIDARMLTRASKLVSAVSSFPVQYNKAIVGRNAFAHESGIHQ
DGMLKNAQTYEIMTPESVGVHKTSLVMGKHSGRHAFKEKLKELGYELGENALEEAFARFKDLADRKKIVYDEDLIALVDD
EIIHAHDRIKLVALTVIAGTKGPQSAALTLTIDGVEQTHQATGNGPIDAIFNAVQALVPHGAVLELYQVHAVTKGTDAQA
EVSVRLSEDGKTVTARGADPDTLVASAKAYIAALNKLVVRRHKSRPDDSLAG
>Mature_531_residues
TTNKSSEPAMGSAIQETANTEQIIIFDTTLRDGEQSPGASMNLDEKIEVAELLDAMGVDVIEAGFPVTSEGDFEAVSAIS
RRVKRASVAGLARAAARDIDRCAEALRDAVRPRMHVFISTSPLHMKYKLQKEPGEVLDMISTYVGRARNRIDDIEWSAED
GTRTEIDFLCRCVEAAIKAGARTINIPDTVGYTIPEEYQALFETVRTRVPNSDQAIFSVHCHNDLGLAVANTLAGIKGGA
RQVECTINGLGERAGNAAMEEVVMALRVRADALAYHSNIDARMLTRASKLVSAVSSFPVQYNKAIVGRNAFAHESGIHQD
GMLKNAQTYEIMTPESVGVHKTSLVMGKHSGRHAFKEKLKELGYELGENALEEAFARFKDLADRKKIVYDEDLIALVDDE
IIHAHDRIKLVALTVIAGTKGPQSAALTLTIDGVEQTHQATGNGPIDAIFNAVQALVPHGAVLELYQVHAVTKGTDAQAE
VSVRLSEDGKTVTARGADPDTLVASAKAYIAALNKLVVRRHKSRPDDSLAG

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=502, Percent_Identity=46.4143426294821, Blast_Score=441, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6320071, Length=391, Percent_Identity=27.8772378516624, Blast_Score=152, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6320019, Length=374, Percent_Identity=28.3422459893048, Blast_Score=151, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6324225, Length=536, Percent_Identity=26.1194029850746, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324682, Length=537, Percent_Identity=24.7672253258845, Blast_Score=111, Evalue=3e-25,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 57535; Mature: 57404

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNKSSEPAMGSAIQETANTEQIIIFDTTLRDGEQSPGASMNLDEKIEVAELLDAMGVD
CCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCH
VIEAGFPVTSEGDFEAVSAISRRVKRASVAGLARAAARDIDRCAEALRDAVRPRMHVFIS
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
TSPLHMKYKLQKEPGEVLDMISTYVGRARNRIDDIEWSAEDGTRTEIDFLCRCVEAAIKA
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
GARTINIPDTVGYTIPEEYQALFETVRTRVPNSDQAIFSVHCHNDLGLAVANTLAGIKGG
CCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCC
ARQVECTINGLGERAGNAAMEEVVMALRVRADALAYHSNIDARMLTRASKLVSAVSSFPV
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
QYNKAIVGRNAFAHESGIHQDGMLKNAQTYEIMTPESVGVHKTSLVMGKHSGRHAFKEKL
HHCCEEECCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHEEEECCCCCHHHHHHHH
KELGYELGENALEEAFARFKDLADRKKIVYDEDLIALVDDEIIHAHDRIKLVALTVIAGT
HHHHHHHCHHHHHHHHHHHHHHHHHCCEEECCCHHEEHHHHHHHHCCCEEEEEEEEEECC
KGPQSAALTLTIDGVEQTHQATGNGPIDAIFNAVQALVPHGAVLELYQVHAVTKGTDAQA
CCCCCEEEEEEECCHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCE
EVSVRLSEDGKTVTARGADPDTLVASAKAYIAALNKLVVRRHKSRPDDSLAG
EEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TTNKSSEPAMGSAIQETANTEQIIIFDTTLRDGEQSPGASMNLDEKIEVAELLDAMGVD
CCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCH
VIEAGFPVTSEGDFEAVSAISRRVKRASVAGLARAAARDIDRCAEALRDAVRPRMHVFIS
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
TSPLHMKYKLQKEPGEVLDMISTYVGRARNRIDDIEWSAEDGTRTEIDFLCRCVEAAIKA
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
GARTINIPDTVGYTIPEEYQALFETVRTRVPNSDQAIFSVHCHNDLGLAVANTLAGIKGG
CCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCC
ARQVECTINGLGERAGNAAMEEVVMALRVRADALAYHSNIDARMLTRASKLVSAVSSFPV
CEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
QYNKAIVGRNAFAHESGIHQDGMLKNAQTYEIMTPESVGVHKTSLVMGKHSGRHAFKEKL
HHCCEEECCCCHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHEEEECCCCCHHHHHHHH
KELGYELGENALEEAFARFKDLADRKKIVYDEDLIALVDDEIIHAHDRIKLVALTVIAGT
HHHHHHHCHHHHHHHHHHHHHHHHHCCEEECCCHHEEHHHHHHHHCCCEEEEEEEEEECC
KGPQSAALTLTIDGVEQTHQATGNGPIDAIFNAVQALVPHGAVLELYQVHAVTKGTDAQA
CCCCCEEEEEEECCHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCE
EVSVRLSEDGKTVTARGADPDTLVASAKAYIAALNKLVVRRHKSRPDDSLAG
EEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA