Click here to switch to the map view.

The map label for this gene is nifN [H]

Identifier: 182677473

GI number: 182677473

Start: 540154

End: 541533

Strand: Direct

Name: nifN [H]

Synonym: Bind_0477

Alternate gene names: 182677473

Gene position: 540154-541533 (Clockwise)

Preceding gene: 182677472

Following gene: 182677474

Centisome position: 12.95

GC content: 53.55

Gene sequence:

>1380_bases
ATGGCTATTGTTAAATTCAATAAAAAAGCCTGTACGGTCAATCCTTTGAAGATGAGCCAGCCGGTCGGCGGCGCGCTCGC
CTTCATGGGGATCAAGGATGCCATGCCGCTGTTTCACGGCTCACAGGGCTGCACCTCCTTCGGGTTGGTGCTGTTCGTGC
GGCATTTCAAGGAAGCCATTCCGTTGCAGACAACGGCTATGAGCGAAGTGGCGACGGTTCTCGGCGGCTATGAAAATGTC
GAGCAGGCGATCGTCAATATTTCGAACAAACAGAAAGCCAGTCTTATCGGTATTTGTTCGACCGGCGTCACCGAGACGAA
GGGCGATGATGTCGATGCCTATATCAAGCTCGTGCGGCAACAGCATCCCGAGCTTGATCATGTATCGCTTGTCTATGTGT
CAACGCCCGACTTCAAAGATGCCTTCCAGGATGGTTGGGAGAAGACGGTCGCCAAGATCATCGAGACTTTGGTGAAGCCG
CGTCCGGCCGATGCGCCGCGTAATTCACGCAAGATTGCTGTGCTTCCAGGCAGTCATCTGACGCCGGGCGATCTTGACGA
ATTACGGGACATTATCGAGGCTTTCGGTTTCGATCCTTTGTTCCTGCCTGATCTTGCTGGCTCGCTTGATGGGCATATTC
CAGAGGATTTTACGCCCACGACCTTGGGTGGTATTTCTCTGGATGAGATCGCCGATCTTGGAGATGCCTTTTATACTATT
GCCATCGGGTCGCATATGCGTACCGCGGCCGAGGCTTTGAAGGAAAAGGCCGGCGTTCCCTATCAGCTATTCGATCGGAT
GATCGGGCTCTTGCCGACGGATGATTTCATTGCCTTCCTGACGCAGATCAGCGGCAAGCCGGTGCCTAACAAGATCAAAA
GGCAGCGCGGCCAATTGGTCGATGCGATGCTCGATGGGCATTTCCATACCGGAGGCAAGAAAATCGCCATAGGTCTGGAG
CCTGATTTCCTTTACGATCTGTCGAATTTCCTTGCCGATATGGGATGTCAGATCGAGGTTGCTGTCACGACGAACCAGAC
GGCCATTCTCGATCAAGTACGCTCGGAAGAGGTTCTTGTCGGCGATCTCGAAGATTTCGAGACCCTAGCGCCGGGTTGTG
ACCTCCTCATTACGCATGCGCATGGGCGCCAGGCGTCAGAGCGTTTGCATATTCCTTTGTTCCGCATGGGGCTTCCGATT
TTCGATCGACTCGGTGCGGGTCACATCACGTCGGTCGGCTATCGCGGCGCGCGTGATCTCATTTTTAAGATCGCCAATAT
ATTCATCGAAAACATGCATGTCGCCAAACCTGATACCTGGTATCCACATCCTGGGGCAGAAGAAGGTCACGCATCAGCGG
ATAAGCTCACGACCCATTGA

Upstream 100 bases:

>100_bases
GGGCAGCGGTTGTGGCAGCTGCGGCGAGACGATCGAGGAGATCCTTGGCGAGGTTCTTGCTTCTATTCCGCCTATCGCGG
CAGAGTGATGAGGCATCGTC

Downstream 100 bases:

>100_bases
AGGAAGAAATAGGAATAATCGGGCGCAGGAGACGAGATGAAAGTAGCATTTGCGACTCAGGATCTTGAGCGGGTTGACGC
TCATTTCGGCTGGGCGAAAA

Product: nitrogenase molybdenum-iron cofactor biosynthesis protein NifN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MAIVKFNKKACTVNPLKMSQPVGGALAFMGIKDAMPLFHGSQGCTSFGLVLFVRHFKEAIPLQTTAMSEVATVLGGYENV
EQAIVNISNKQKASLIGICSTGVTETKGDDVDAYIKLVRQQHPELDHVSLVYVSTPDFKDAFQDGWEKTVAKIIETLVKP
RPADAPRNSRKIAVLPGSHLTPGDLDELRDIIEAFGFDPLFLPDLAGSLDGHIPEDFTPTTLGGISLDEIADLGDAFYTI
AIGSHMRTAAEALKEKAGVPYQLFDRMIGLLPTDDFIAFLTQISGKPVPNKIKRQRGQLVDAMLDGHFHTGGKKIAIGLE
PDFLYDLSNFLADMGCQIEVAVTTNQTAILDQVRSEEVLVGDLEDFETLAPGCDLLITHAHGRQASERLHIPLFRMGLPI
FDRLGAGHITSVGYRGARDLIFKIANIFIENMHVAKPDTWYPHPGAEEGHASADKLTTH

Sequences:

>Translated_459_residues
MAIVKFNKKACTVNPLKMSQPVGGALAFMGIKDAMPLFHGSQGCTSFGLVLFVRHFKEAIPLQTTAMSEVATVLGGYENV
EQAIVNISNKQKASLIGICSTGVTETKGDDVDAYIKLVRQQHPELDHVSLVYVSTPDFKDAFQDGWEKTVAKIIETLVKP
RPADAPRNSRKIAVLPGSHLTPGDLDELRDIIEAFGFDPLFLPDLAGSLDGHIPEDFTPTTLGGISLDEIADLGDAFYTI
AIGSHMRTAAEALKEKAGVPYQLFDRMIGLLPTDDFIAFLTQISGKPVPNKIKRQRGQLVDAMLDGHFHTGGKKIAIGLE
PDFLYDLSNFLADMGCQIEVAVTTNQTAILDQVRSEEVLVGDLEDFETLAPGCDLLITHAHGRQASERLHIPLFRMGLPI
FDRLGAGHITSVGYRGARDLIFKIANIFIENMHVAKPDTWYPHPGAEEGHASADKLTTH
>Mature_458_residues
AIVKFNKKACTVNPLKMSQPVGGALAFMGIKDAMPLFHGSQGCTSFGLVLFVRHFKEAIPLQTTAMSEVATVLGGYENVE
QAIVNISNKQKASLIGICSTGVTETKGDDVDAYIKLVRQQHPELDHVSLVYVSTPDFKDAFQDGWEKTVAKIIETLVKPR
PADAPRNSRKIAVLPGSHLTPGDLDELRDIIEAFGFDPLFLPDLAGSLDGHIPEDFTPTTLGGISLDEIADLGDAFYTIA
IGSHMRTAAEALKEKAGVPYQLFDRMIGLLPTDDFIAFLTQISGKPVPNKIKRQRGQLVDAMLDGHFHTGGKKIAIGLEP
DFLYDLSNFLADMGCQIEVAVTTNQTAILDQVRSEEVLVGDLEDFETLAPGCDLLITHAHGRQASERLHIPLFRMGLPIF
DRLGAGHITSVGYRGARDLIFKIANIFIENMHVAKPDTWYPHPGAEEGHASADKLTTH

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005975 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 50034; Mature: 49903

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIVKFNKKACTVNPLKMSQPVGGALAFMGIKDAMPLFHGSQGCTSFGLVLFVRHFKEAI
CEEEEECCCCEEECCHHCCCCCCCCEEEHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHC
PLQTTAMSEVATVLGGYENVEQAIVNISNKQKASLIGICSTGVTETKGDDVDAYIKLVRQ
CCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
QHPELDHVSLVYVSTPDFKDAFQDGWEKTVAKIIETLVKPRPADAPRNSRKIAVLPGSHL
HCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCC
TPGDLDELRDIIEAFGFDPLFLPDLAGSLDGHIPEDFTPTTLGGISLDEIADLGDAFYTI
CCCCHHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCHHEEEE
AIGSHMRTAAEALKEKAGVPYQLFDRMIGLLPTDDFIAFLTQISGKPVPNKIKRQRGQLV
EECHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
DAMLDGHFHTGGKKIAIGLEPDFLYDLSNFLADMGCQIEVAVTTNQTAILDQVRSEEVLV
HHHHCCCEECCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCCCEEE
GDLEDFETLAPGCDLLITHAHGRQASERLHIPLFRMGLPIFDRLGAGHITSVGYRGARDL
ECHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHCCCHHHHCCCCCCHHCCCCCHHHH
IFKIANIFIENMHVAKPDTWYPHPGAEEGHASADKLTTH
HHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
AIVKFNKKACTVNPLKMSQPVGGALAFMGIKDAMPLFHGSQGCTSFGLVLFVRHFKEAI
EEEEECCCCEEECCHHCCCCCCCCEEEHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHC
PLQTTAMSEVATVLGGYENVEQAIVNISNKQKASLIGICSTGVTETKGDDVDAYIKLVRQ
CCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
QHPELDHVSLVYVSTPDFKDAFQDGWEKTVAKIIETLVKPRPADAPRNSRKIAVLPGSHL
HCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCC
TPGDLDELRDIIEAFGFDPLFLPDLAGSLDGHIPEDFTPTTLGGISLDEIADLGDAFYTI
CCCCHHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCHHEEEE
AIGSHMRTAAEALKEKAGVPYQLFDRMIGLLPTDDFIAFLTQISGKPVPNKIKRQRGQLV
EECHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
DAMLDGHFHTGGKKIAIGLEPDFLYDLSNFLADMGCQIEVAVTTNQTAILDQVRSEEVLV
HHHHCCCEECCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCCCEEE
GDLEDFETLAPGCDLLITHAHGRQASERLHIPLFRMGLPIFDRLGAGHITSVGYRGARDL
ECHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHCCCHHHHCCCCCCHHCCCCCHHHH
IFKIANIFIENMHVAKPDTWYPHPGAEEGHASADKLTTH
HHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2266945; 11157954; 12597275 [H]