Click here to switch to the map view.

The map label for this gene is nifE [H]

Identifier: 182677472

GI number: 182677472

Start: 538252

End: 540141

Strand: Direct

Name: nifE [H]

Synonym: Bind_0476

Alternate gene names: 182677472

Gene position: 538252-540141 (Clockwise)

Preceding gene: 182677471

Following gene: 182677473

Centisome position: 12.91

GC content: 55.5

Gene sequence:

>1890_bases
ATGAGCAGTGTTTCCGCCACAATTCAAGACGTTTTCAACGAGCCGGGCTGCGCTAAAAATGCCGGCAAGTCTGAAAAGGA
AAAGAAAAAGGGTTGCACGAAACAATTGCAGCCTGGTGGCGCAGCGGGCGGTTGTGCCTTCGACGGCGCCAAGATCGCCC
TGCAGCCCATTACCGATGTGGCCCATCTTGTTCATGGTCCGATTGCCTGCGAAGGCAATTCCTGGGACAATCGCGGCGCT
TTCTCGTCGGGTTCCACACTCTATCGCACCGGCTTTACAACCGATATCAATGAAACCGATGTGGTGTTCGGTGGCGAGAA
GCGGCTCTACAAGAGCATCAAGGAAATCATCGAAAAATATAATCCGCCGTCTGTCTTCGTCTATCAGACTTGCGTCCCGG
CCATGATCGGCGACGATATCGACGCGGTGTGCAAGGCGGCTTCGAAAAAATTCGGCAAGCCGATTGTGCCTGTGAATTCC
CCCGGCTTTGTCGGTCCGAAGAATCTCGGTAACAAGCTCGCGGGTGAGGCTCTGCTTGCTCACGTGATTGGCACTATCGA
GCCCGAATATACGACGCCTTATGATGTGAATATCATCGGCGAATATAATCTGGCTGGTGAAATGTGGCAGGTTGAGCCGC
TCCTGAAGGAGATCGGCTTCCGTATCATTTCCTGCATTTCCGGCGATGCGAAATATAATGAAGTCGCGCAGGCGCATCGC
GCCAAGGCGACGATGATGGTCTGCTCCAAGGCCATGATCAATATTACGCGCAAGCTCGAAGATAAATACGGTATTCCCTA
TTTCGAAGGCTCGTTCTACGGCATCGGCGATATGAGCGATTCCATTCGGCAGCTTGCCCAATTGGTGATCGATCAGGGCG
CGCCGCCGGAATTCATGGATCGCGCTGAAGCGGTGATTGCCCGTGAAGAAAAGAAGGCCTGGGAGCGCATGGCAGCCTAT
ACGCCGCGCCTCAAGGGCAAGAAGGTTCTGCTCATCACCGGCGGTGTGAAGTCCTGGTCCGTGGTTGCCGCCTTGCAAGA
AGTCGGTCTCGAAATCGTTGGCACCAGTGTCAAGAAGTCGACGAAGGAAGATAAAGAGCGCATCAAGGAATTGATGGGTG
AGGATGCTCACGCCTTCGACGATATGTCGCCGCGCGAAATGTATAAAATGCTGAAGGATGCCAAGGCGGATATCATGTTG
TCCGGCGGCCGTTCGCAGTTCATCGCGCTGAAAGCCTCTATGCCGTGGCTGGATATCAACCAGGAACGTCATCACGCCTA
TGCCGGCTATGAAGGCATGGTTGATCTTGTGCGCGAAATCGACAAAGCGCTTTACAATCCGATCTGGGAACAAGTGCGCA
AGATTGCGCCGTGGGAAAATCCCGCGGAAAGCTGGCAGGCCAAGGCTGATGCCGAAGCCGCGCGCGAGGCCGCTGAATTG
GCTGCCAACCCGGCCAAAGCGGAGGAGAAGCGGCGCTCGAAGAAGATTTGCAAGTGCAAATCCGTGGATCTCGGCACGAT
CGAGGACGCTATTAAGGCGAATAATCTGACAACCGCCAAGCAGGTCACCGAAATCACCCATGCGGGTGGTGGCTGCACGG
GCTGTGTCGGCACGATCGAAGGCATCATCGAGGAGCTTCTCAAGCCGGCTGATGCTGTCGCGCTCGATCCCGCGCAGGCC
GAAGAGAAGCGTCGTGCCAAAAAAGTGTGCAACTGCAAAGAAGTCACGGTGGGCACGATTGAAGATGCCATTCGTGATAA
AGGATTGAGCACAGCTGCTCAGGTGACGGCCGCCACGGAAGCGGGCAGCGGTTGTGGCAGCTGCGGCGAGACGATCGAGG
AGATCCTTGGCGAGGTTCTTGCTTCTATTCCGCCTATCGCGGCAGAGTGA

Upstream 100 bases:

>100_bases
CGATAATGAAGCCTCACTGGGCAGTCGCGCTGCCAAAATGAGCGTGCGCGGCATCGAAGTCTGGCCGGTATGCCGGTCGG
AAAGCGCCGAGGAGAATTCG

Downstream 100 bases:

>100_bases
TGAGGCATCGTCATGGCTATTGTTAAATTCAATAAAAAAGCCTGTACGGTCAATCCTTTGAAGATGAGCCAGCCGGTCGG
CGGCGCGCTCGCCTTCATGG

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MSSVSATIQDVFNEPGCAKNAGKSEKEKKKGCTKQLQPGGAAGGCAFDGAKIALQPITDVAHLVHGPIACEGNSWDNRGA
FSSGSTLYRTGFTTDINETDVVFGGEKRLYKSIKEIIEKYNPPSVFVYQTCVPAMIGDDIDAVCKAASKKFGKPIVPVNS
PGFVGPKNLGNKLAGEALLAHVIGTIEPEYTTPYDVNIIGEYNLAGEMWQVEPLLKEIGFRIISCISGDAKYNEVAQAHR
AKATMMVCSKAMINITRKLEDKYGIPYFEGSFYGIGDMSDSIRQLAQLVIDQGAPPEFMDRAEAVIAREEKKAWERMAAY
TPRLKGKKVLLITGGVKSWSVVAALQEVGLEIVGTSVKKSTKEDKERIKELMGEDAHAFDDMSPREMYKMLKDAKADIML
SGGRSQFIALKASMPWLDINQERHHAYAGYEGMVDLVREIDKALYNPIWEQVRKIAPWENPAESWQAKADAEAAREAAEL
AANPAKAEEKRRSKKICKCKSVDLGTIEDAIKANNLTTAKQVTEITHAGGGCTGCVGTIEGIIEELLKPADAVALDPAQA
EEKRRAKKVCNCKEVTVGTIEDAIRDKGLSTAAQVTAATEAGSGCGSCGETIEEILGEVLASIPPIAAE

Sequences:

>Translated_629_residues
MSSVSATIQDVFNEPGCAKNAGKSEKEKKKGCTKQLQPGGAAGGCAFDGAKIALQPITDVAHLVHGPIACEGNSWDNRGA
FSSGSTLYRTGFTTDINETDVVFGGEKRLYKSIKEIIEKYNPPSVFVYQTCVPAMIGDDIDAVCKAASKKFGKPIVPVNS
PGFVGPKNLGNKLAGEALLAHVIGTIEPEYTTPYDVNIIGEYNLAGEMWQVEPLLKEIGFRIISCISGDAKYNEVAQAHR
AKATMMVCSKAMINITRKLEDKYGIPYFEGSFYGIGDMSDSIRQLAQLVIDQGAPPEFMDRAEAVIAREEKKAWERMAAY
TPRLKGKKVLLITGGVKSWSVVAALQEVGLEIVGTSVKKSTKEDKERIKELMGEDAHAFDDMSPREMYKMLKDAKADIML
SGGRSQFIALKASMPWLDINQERHHAYAGYEGMVDLVREIDKALYNPIWEQVRKIAPWENPAESWQAKADAEAAREAAEL
AANPAKAEEKRRSKKICKCKSVDLGTIEDAIKANNLTTAKQVTEITHAGGGCTGCVGTIEGIIEELLKPADAVALDPAQA
EEKRRAKKVCNCKEVTVGTIEDAIRDKGLSTAAQVTAATEAGSGCGSCGETIEEILGEVLASIPPIAAE
>Mature_628_residues
SSVSATIQDVFNEPGCAKNAGKSEKEKKKGCTKQLQPGGAAGGCAFDGAKIALQPITDVAHLVHGPIACEGNSWDNRGAF
SSGSTLYRTGFTTDINETDVVFGGEKRLYKSIKEIIEKYNPPSVFVYQTCVPAMIGDDIDAVCKAASKKFGKPIVPVNSP
GFVGPKNLGNKLAGEALLAHVIGTIEPEYTTPYDVNIIGEYNLAGEMWQVEPLLKEIGFRIISCISGDAKYNEVAQAHRA
KATMMVCSKAMINITRKLEDKYGIPYFEGSFYGIGDMSDSIRQLAQLVIDQGAPPEFMDRAEAVIAREEKKAWERMAAYT
PRLKGKKVLLITGGVKSWSVVAALQEVGLEIVGTSVKKSTKEDKERIKELMGEDAHAFDDMSPREMYKMLKDAKADIMLS
GGRSQFIALKASMPWLDINQERHHAYAGYEGMVDLVREIDKALYNPIWEQVRKIAPWENPAESWQAKADAEAAREAAELA
ANPAKAEEKRRSKKICKCKSVDLGTIEDAIKANNLTTAKQVTEITHAGGGCTGCVGTIEGIIEELLKPADAVALDPAQAE
EKRRAKKVCNCKEVTVGTIEDAIRDKGLSTAAQVTAATEAGSGCGSCGETIEEILGEVLASIPPIAAE

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 67936; Mature: 67805

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVSATIQDVFNEPGCAKNAGKSEKEKKKGCTKQLQPGGAAGGCAFDGAKIALQPITDV
CCCHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHCCCCCCCCCCCCCCCEEEEHHHHHH
AHLVHGPIACEGNSWDNRGAFSSGSTLYRTGFTTDINETDVVFGGEKRLYKSIKEIIEKY
HHHHCCCEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHHC
NPPSVFVYQTCVPAMIGDDIDAVCKAASKKFGKPIVPVNSPGFVGPKNLGNKLAGEALLA
CCCCEEEEHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HVIGTIEPEYTTPYDVNIIGEYNLAGEMWQVEPLLKEIGFRIISCISGDAKYNEVAQAHR
HHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AKATMMVCSKAMINITRKLEDKYGIPYFEGSFYGIGDMSDSIRQLAQLVIDQGAPPEFMD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCCHHHHH
RAEAVIAREEKKAWERMAAYTPRLKGKKVLLITGGVKSWSVVAALQEVGLEIVGTSVKKS
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHCCCHHHHH
TKEDKERIKELMGEDAHAFDDMSPREMYKMLKDAKADIMLSGGRSQFIALKASMPWLDIN
HHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCEECC
QERHHAYAGYEGMVDLVREIDKALYNPIWEQVRKIAPWENPAESWQAKADAEAAREAAEL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AANPAKAEEKRRSKKICKCKSVDLGTIEDAIKANNLTTAKQVTEITHAGGGCTGCVGTIE
HCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHH
GIIEELLKPADAVALDPAQAEEKRRAKKVCNCKEVTVGTIEDAIRDKGLSTAAQVTAATE
HHHHHHHCCCHHEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
AGSGCGSCGETIEEILGEVLASIPPIAAE
CCCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSVSATIQDVFNEPGCAKNAGKSEKEKKKGCTKQLQPGGAAGGCAFDGAKIALQPITDV
CCHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHCCCCCCCCCCCCCCCEEEEHHHHHH
AHLVHGPIACEGNSWDNRGAFSSGSTLYRTGFTTDINETDVVFGGEKRLYKSIKEIIEKY
HHHHCCCEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHHC
NPPSVFVYQTCVPAMIGDDIDAVCKAASKKFGKPIVPVNSPGFVGPKNLGNKLAGEALLA
CCCCEEEEHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HVIGTIEPEYTTPYDVNIIGEYNLAGEMWQVEPLLKEIGFRIISCISGDAKYNEVAQAHR
HHHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AKATMMVCSKAMINITRKLEDKYGIPYFEGSFYGIGDMSDSIRQLAQLVIDQGAPPEFMD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCCHHHHH
RAEAVIAREEKKAWERMAAYTPRLKGKKVLLITGGVKSWSVVAALQEVGLEIVGTSVKKS
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCHHHCCCHHHHH
TKEDKERIKELMGEDAHAFDDMSPREMYKMLKDAKADIMLSGGRSQFIALKASMPWLDIN
HHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCEECC
QERHHAYAGYEGMVDLVREIDKALYNPIWEQVRKIAPWENPAESWQAKADAEAAREAAEL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AANPAKAEEKRRSKKICKCKSVDLGTIEDAIKANNLTTAKQVTEITHAGGGCTGCVGTIE
HCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHH
GIIEELLKPADAVALDPAQAEEKRRAKKVCNCKEVTVGTIEDAIRDKGLSTAAQVTAATE
HHHHHHHCCCHHEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
AGSGCGSCGETIEEILGEVLASIPPIAAE
CCCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2266945; 11157954; 12597275 [H]