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The map label for this gene is nifS1 [H]

Identifier: 182677399

GI number: 182677399

Start: 461390

End: 462526

Strand: Direct

Name: nifS1 [H]

Synonym: Bind_0403

Alternate gene names: 182677399

Gene position: 461390-462526 (Clockwise)

Preceding gene: 182677398

Following gene: 182677400

Centisome position: 11.06

GC content: 60.25

Gene sequence:

>1137_bases
ATGATCTATCTCGATAATAATGCCACGACGGCTGTGGCGCCCGAAGCGATCGAAGCCATGGCCGAATGCATGCGCAGTGG
CCCGACCAATCCTTCGAGCAAGCACAAGGCAGGCGAGTGCAGCAAGGAATCCGTCATGCAGGCGCGTGGCGAGGTTGCGC
GCTTTCTGTCCTGTTCGCCGCCAGAAATCGTCTTCACGAGCTGCGGCACCGAATCCAACCATATGGCCATTTTGGGCGCG
CTCGCTCTCAATCCCGATAAAAAACATATTGTCGCGAGCGCCGTCGAACATCCGTCCTGCCTGGAGCTTTTGCGCCATCT
CGAAAAGCAGGGCGTCGCGGTGAGCTATGTGCCGGTGGATGCGCAAGGCCAATTGGATGCCGCCGCGGTCGCTTCGAGCA
TTCGGCCCGATACGGCGCTGGTGACGGTCATGTGGGCGAATAACGAGACCGGCGTCATTTTCCCCATCGAGGCCATTGCC
GCGATCACCAAGGAAAAAGGCGTTCTGTTCCATACCGACGCGGTGCAGGCGGTCAGCAAGATTCCGGTCGATCTCAAACA
AGTGCCGGTCGATCTTTTGTCGCTCTCCGGCCATAAGCTGCATGGCCCGACGGGCATTGGTGTTCTCTTTGTGCGCAAGG
GGATCAAACTTCCTTCGCAGATCTTCGGCCACCAGGAACGGGCGCGTCGCGGAGGCACGGAGAATGTTCCGGGCATTATC
GGTCTCGGCAAGGCGGCGGTGCTCGCGCAGAAGGAATTGGCTGAACAGCAGGTTCAGCTCGCCGCCCTGCGTGACCGTTT
GGAGGCGGCGGTGCTTGCCGCCGTTCCGATCGCGACAGTCAATGGCGCCGGTGCCAAGCGTGTCTCGAATACGACCAATA
TCAGGTTCGGCAATCTCGAGGCCGAACTGCTTCTCGAACGTCTTGATCGCGCCGGTATTTGTGCGTCCGCTGGCGCTGCG
TGTACCTCGGCGGGGACGGAGCCGTCGCATGTGTTGCTGGCGATGGGGGTTGACGAACGCGGCGCTTTGTCGTCGATAAG
ATTCTCCCTGGGCCGTGATAATACAGCGGCGGATATAGACACGCTTCTCGAAGTGCTGCCCGGGATCGTGAACGAAGCAG
CCGCCATCGCCGCTTGA

Upstream 100 bases:

>100_bases
AAATTGCATCCTTTAGGAGATAATCATTTTGGCGGATGGATTTGCGCGCCCGTTCCACTCTTGGAGAGGATTGGGCGGCG
AGAGATGACAGGCCAGTGAC

Downstream 100 bases:

>100_bases
GGCTTGCGCGGCTGTGGCATCACATCCAAAAGGAAACACTTTTGGAGTCGTGATGCGTTTTCAAAAGCCGGAGCGGATGT
CCGATGGATTTCGGGCTTTG

Product: class V aminotransferase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MIYLDNNATTAVAPEAIEAMAECMRSGPTNPSSKHKAGECSKESVMQARGEVARFLSCSPPEIVFTSCGTESNHMAILGA
LALNPDKKHIVASAVEHPSCLELLRHLEKQGVAVSYVPVDAQGQLDAAAVASSIRPDTALVTVMWANNETGVIFPIEAIA
AITKEKGVLFHTDAVQAVSKIPVDLKQVPVDLLSLSGHKLHGPTGIGVLFVRKGIKLPSQIFGHQERARRGGTENVPGII
GLGKAAVLAQKELAEQQVQLAALRDRLEAAVLAAVPIATVNGAGAKRVSNTTNIRFGNLEAELLLERLDRAGICASAGAA
CTSAGTEPSHVLLAMGVDERGALSSIRFSLGRDNTAADIDTLLEVLPGIVNEAAAIAA

Sequences:

>Translated_378_residues
MIYLDNNATTAVAPEAIEAMAECMRSGPTNPSSKHKAGECSKESVMQARGEVARFLSCSPPEIVFTSCGTESNHMAILGA
LALNPDKKHIVASAVEHPSCLELLRHLEKQGVAVSYVPVDAQGQLDAAAVASSIRPDTALVTVMWANNETGVIFPIEAIA
AITKEKGVLFHTDAVQAVSKIPVDLKQVPVDLLSLSGHKLHGPTGIGVLFVRKGIKLPSQIFGHQERARRGGTENVPGII
GLGKAAVLAQKELAEQQVQLAALRDRLEAAVLAAVPIATVNGAGAKRVSNTTNIRFGNLEAELLLERLDRAGICASAGAA
CTSAGTEPSHVLLAMGVDERGALSSIRFSLGRDNTAADIDTLLEVLPGIVNEAAAIAA
>Mature_378_residues
MIYLDNNATTAVAPEAIEAMAECMRSGPTNPSSKHKAGECSKESVMQARGEVARFLSCSPPEIVFTSCGTESNHMAILGA
LALNPDKKHIVASAVEHPSCLELLRHLEKQGVAVSYVPVDAQGQLDAAAVASSIRPDTALVTVMWANNETGVIFPIEAIA
AITKEKGVLFHTDAVQAVSKIPVDLKQVPVDLLSLSGHKLHGPTGIGVLFVRKGIKLPSQIFGHQERARRGGTENVPGII
GLGKAAVLAQKELAEQQVQLAALRDRLEAAVLAAVPIATVNGAGAKRVSNTTNIRFGNLEAELLLERLDRAGICASAGAA
CTSAGTEPSHVLLAMGVDERGALSSIRFSLGRDNTAADIDTLLEVLPGIVNEAAAIAA

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=368, Percent_Identity=39.4021739130435, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI156713448, Length=406, Percent_Identity=36.6995073891626, Blast_Score=216, Evalue=3e-56,
Organism=Escherichia coli, GI48994898, Length=372, Percent_Identity=40.5913978494624, Blast_Score=267, Evalue=7e-73,
Organism=Escherichia coli, GI1789175, Length=386, Percent_Identity=26.6839378238342, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1787970, Length=347, Percent_Identity=25.0720461095101, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI25143064, Length=370, Percent_Identity=40, Blast_Score=258, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI17533177, Length=318, Percent_Identity=34.9056603773585, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319831, Length=368, Percent_Identity=41.0326086956522, Blast_Score=261, Evalue=9e-71,
Organism=Drosophila melanogaster, GI20129463, Length=364, Percent_Identity=39.2857142857143, Blast_Score=256, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 39469; Mature: 39469

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDNNATTAVAPEAIEAMAECMRSGPTNPSSKHKAGECSKESVMQARGEVARFLSCSP
CEEECCCCCCEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
PEIVFTSCGTESNHMAILGALALNPDKKHIVASAVEHPSCLELLRHLEKQGVAVSYVPVD
CCEEEEECCCCCCCEEEEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECC
AQGQLDAAAVASSIRPDTALVTVMWANNETGVIFPIEAIAAITKEKGVLFHTDAVQAVSK
CCCCCHHHHHHHCCCCCCEEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEHHHHHHHHH
IPVDLKQVPVDLLSLSGHKLHGPTGIGVLFVRKGIKLPSQIFGHQERARRGGTENVPGII
CCCCHHHCCHHHHHCCCCEECCCCCCCCEEECCCCCCCHHHHCCHHHHHCCCCCCCCCEE
GLGKAAVLAQKELAEQQVQLAALRDRLEAAVLAAVPIATVNGAGAKRVSNTTNIRFGNLE
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHCCCCCCEEECCCH
AELLLERLDRAGICASAGAACTSAGTEPSHVLLAMGVDERGALSSIRFSLGRDNTAADID
HHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCHHHHH
TLLEVLPGIVNEAAAIAA
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIYLDNNATTAVAPEAIEAMAECMRSGPTNPSSKHKAGECSKESVMQARGEVARFLSCSP
CEEECCCCCCEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
PEIVFTSCGTESNHMAILGALALNPDKKHIVASAVEHPSCLELLRHLEKQGVAVSYVPVD
CCEEEEECCCCCCCEEEEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECC
AQGQLDAAAVASSIRPDTALVTVMWANNETGVIFPIEAIAAITKEKGVLFHTDAVQAVSK
CCCCCHHHHHHHCCCCCCEEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEHHHHHHHHH
IPVDLKQVPVDLLSLSGHKLHGPTGIGVLFVRKGIKLPSQIFGHQERARRGGTENVPGII
CCCCHHHCCHHHHHCCCCEECCCCCCCCEEECCCCCCCHHHHCCHHHHHCCCCCCCCCEE
GLGKAAVLAQKELAEQQVQLAALRDRLEAAVLAAVPIATVNGAGAKRVSNTTNIRFGNLE
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHCCCCCCEEECCCH
AELLLERLDRAGICASAGAACTSAGTEPSHVLLAMGVDERGALSSIRFSLGRDNTAADID
HHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCHHHHH
TLLEVLPGIVNEAAAIAA
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]