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The map label for this gene is dacF [H]

Identifier: 182677354

GI number: 182677354

Start: 404866

End: 406482

Strand: Direct

Name: dacF [H]

Synonym: Bind_0357

Alternate gene names: 182677354

Gene position: 404866-406482 (Clockwise)

Preceding gene: 182677347

Following gene: 182677355

Centisome position: 9.71

GC content: 61.78

Gene sequence:

>1617_bases
ATGTCTCCTTTCACTCCAGGGCCGCAGAGCGGTCTCCACCGCAAGGGTTTGCGCGGACACGGTACGGAACGCCCGCGACG
CTCCCTCTGGCAACGTTTTCGGCTTCATCGCTTTTCATCGCGCCTGCTCCACGGCGGGCTTGCGATCGCTGCTGTCGCCA
CCACGACGCTCACCGACACCGCACAGGCTGCCCCAGGCCTCGTCATCGATCTCGCGTCCGGCAATGTGATCTACCAGTCG
GAAGCCACGCGACCCTGGTATCCCGCCTCCCTGACCAAATTGATGACGCTTTATGTGGCTCTCCAGGCGGTGCGCGATCA
TCGCATCGGGTTTGACACGCCGCTTGTCGTTTCGGCGCGCGCCCATGCCATGCCGCCCTCCAAGATGGGTTTCAAGCCCG
GCACTCAGGTGACGCTTGGCAATGCGCTCAAAATGCTGATGGTCAAATCGGCGAATGATATTGCGATCACCGTTGCGGAG
GGAATTGCCGGCTCGGTCGAGGCCTTTGCCGAGGACATGAATGCGCAAGCCGCCGATCTTGGCCTCAGGCAGTCGCATTT
CGTCAATCCGAACGGTCTGCATAACGAGCAGCATTATTCCTCGGCGAGGGATATGGCGTTGCTCGCCCGTGCTCTCTATC
TGCGTTTCCCGCAATATGCCTCGCTCTATAATATTGGCGCCTTGCGGCTCGGCAATCAGATCATCCAGAACCACAATAAT
CTGCTCGGACGCTATCCTGGTGTCGATGGCATGAAAACCGGTTACACCTGCACCGCGGGCTTCAACATTGTCGCCAGCGC
GGAACAAAGCGGCCGCCGTGTGATTGTCGTCGTGCTTGGCTCGCCCAATGTCGAAACGCGCACGATCAAGGCTGCCGCTT
TGTTCGACCGGGCCTTCAGCAATATTGACCATGCTTTTGTCACGCTGGACAATTTGCCTGTGCCGCCGGATATGCTGCCT
TCCCCTTATGATGTCTGCCGCAATCGCGCACAGGTCATGGCGGAAGCCAATGCCGAGATCGACACGTTGATGACCCCCTT
GCAGGCAGGGGCCTTCACACCGCAGCCCGAGCGCGCTTTTCTCTTTGATGCCGATGCCCTCGGCCGCGCGGTCCCGGTCG
CCTCGCGGATTACTTTGGTGCCGCGCCCCGTCTTTGATCCGCAGCCCGTATCAGTCGGTCCCGTTGCCGGCTATTCTGGC
CCTGTGGCCCAGGCGCGCGCGCCGCATACACCTATCGGGAGCGCTCCCTTGCCGGAGCTCGCGAGCGCCTATGCCCCGAA
AGCAGCCGCGCGTCCTCTGCCGTTGATGCCTGATCTCGATGCCGTGCCGTTGAAGCAAACACAGACACCGATTAAGCCAG
CGGTCGTTTTGAAACCGGCGCCCGTGGCTCATCGGATGGCCCATCACGCTCCGGTGTCGCCGCCTCCCGCCCACGCCGCC
GCCACGGTCAAGCCGCCGGTGGTGAAGGCCAAAGTCGTCTCGGTAAAGCCCGTCGTGCTCCTCAAGACGGCCGCTCCGCT
CCCGCCCAAAACCGCGGAAAAGCCCAAAACGGCAGGGAAGCCTGCAGCCAAAGTGAGCGCGAAAACGGCGACGACGGAGA
AGACCGCCGTTCATTGA

Upstream 100 bases:

>100_bases
GCGAGACCTTTGTTCTTCAAGGACATAAGGTGACCGCGAGGAATGCAAGCGATCATGCGGCGGTCTCATAGGGCCGATGC
GCGACCGGGATGGGAAAAGA

Downstream 100 bases:

>100_bases
TACGCAAGCGGCCCTTCAGCTCGTTTCGATCGTCTCAAAAATGAGAAATCCGCTGGCGCCGATCAGCGTCGGTGGGACGT
TAGCCGGGCTGTGCATCCAC

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MSPFTPGPQSGLHRKGLRGHGTERPRRSLWQRFRLHRFSSRLLHGGLAIAAVATTTLTDTAQAAPGLVIDLASGNVIYQS
EATRPWYPASLTKLMTLYVALQAVRDHRIGFDTPLVVSARAHAMPPSKMGFKPGTQVTLGNALKMLMVKSANDIAITVAE
GIAGSVEAFAEDMNAQAADLGLRQSHFVNPNGLHNEQHYSSARDMALLARALYLRFPQYASLYNIGALRLGNQIIQNHNN
LLGRYPGVDGMKTGYTCTAGFNIVASAEQSGRRVIVVVLGSPNVETRTIKAAALFDRAFSNIDHAFVTLDNLPVPPDMLP
SPYDVCRNRAQVMAEANAEIDTLMTPLQAGAFTPQPERAFLFDADALGRAVPVASRITLVPRPVFDPQPVSVGPVAGYSG
PVAQARAPHTPIGSAPLPELASAYAPKAAARPLPLMPDLDAVPLKQTQTPIKPAVVLKPAPVAHRMAHHAPVSPPPAHAA
ATVKPPVVKAKVVSVKPVVLLKTAAPLPPKTAEKPKTAGKPAAKVSAKTATTEKTAVH

Sequences:

>Translated_538_residues
MSPFTPGPQSGLHRKGLRGHGTERPRRSLWQRFRLHRFSSRLLHGGLAIAAVATTTLTDTAQAAPGLVIDLASGNVIYQS
EATRPWYPASLTKLMTLYVALQAVRDHRIGFDTPLVVSARAHAMPPSKMGFKPGTQVTLGNALKMLMVKSANDIAITVAE
GIAGSVEAFAEDMNAQAADLGLRQSHFVNPNGLHNEQHYSSARDMALLARALYLRFPQYASLYNIGALRLGNQIIQNHNN
LLGRYPGVDGMKTGYTCTAGFNIVASAEQSGRRVIVVVLGSPNVETRTIKAAALFDRAFSNIDHAFVTLDNLPVPPDMLP
SPYDVCRNRAQVMAEANAEIDTLMTPLQAGAFTPQPERAFLFDADALGRAVPVASRITLVPRPVFDPQPVSVGPVAGYSG
PVAQARAPHTPIGSAPLPELASAYAPKAAARPLPLMPDLDAVPLKQTQTPIKPAVVLKPAPVAHRMAHHAPVSPPPAHAA
ATVKPPVVKAKVVSVKPVVLLKTAAPLPPKTAEKPKTAGKPAAKVSAKTATTEKTAVH
>Mature_537_residues
SPFTPGPQSGLHRKGLRGHGTERPRRSLWQRFRLHRFSSRLLHGGLAIAAVATTTLTDTAQAAPGLVIDLASGNVIYQSE
ATRPWYPASLTKLMTLYVALQAVRDHRIGFDTPLVVSARAHAMPPSKMGFKPGTQVTLGNALKMLMVKSANDIAITVAEG
IAGSVEAFAEDMNAQAADLGLRQSHFVNPNGLHNEQHYSSARDMALLARALYLRFPQYASLYNIGALRLGNQIIQNHNNL
LGRYPGVDGMKTGYTCTAGFNIVASAEQSGRRVIVVVLGSPNVETRTIKAAALFDRAFSNIDHAFVTLDNLPVPPDMLPS
PYDVCRNRAQVMAEANAEIDTLMTPLQAGAFTPQPERAFLFDADALGRAVPVASRITLVPRPVFDPQPVSVGPVAGYSGP
VAQARAPHTPIGSAPLPELASAYAPKAAARPLPLMPDLDAVPLKQTQTPIKPAVVLKPAPVAHRMAHHAPVSPPPAHAAA
TVKPPVVKAKVVSVKPVVLLKTAAPLPPKTAEKPKTAGKPAAKVSAKTATTEKTAVH

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=266, Percent_Identity=36.8421052631579, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI87082022, Length=241, Percent_Identity=35.6846473029046, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1786851, Length=265, Percent_Identity=36.2264150943396, Blast_Score=127, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 57179; Mature: 57048

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPFTPGPQSGLHRKGLRGHGTERPRRSLWQRFRLHRFSSRLLHGGLAIAAVATTTLTDT
CCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHH
AQAAPGLVIDLASGNVIYQSEATRPWYPASLTKLMTLYVALQAVRDHRIGFDTPLVVSAR
HHCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
AHAMPPSKMGFKPGTQVTLGNALKMLMVKSANDIAITVAEGIAGSVEAFAEDMNAQAADL
CCCCCCHHCCCCCCCCEEHHHHHHHHHEECCCCEEEEEECCCCHHHHHHHHHHCCHHHHC
GLRQSHFVNPNGLHNEQHYSSARDMALLARALYLRFPQYASLYNIGALRLGNQIIQNHNN
CCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
LLGRYPGVDGMKTGYTCTAGFNIVASAEQSGRRVIVVVLGSPNVETRTIKAAALFDRAFS
CEECCCCCCCCCCCEEEECCCEEEEECCCCCCEEEEEEECCCCCCHHEEHHHHHHHHHHH
NIDHAFVTLDNLPVPPDMLPSPYDVCRNRAQVMAEANAEIDTLMTPLQAGAFTPQPERAF
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEE
LFDADALGRAVPVASRITLVPRPVFDPQPVSVGPVAGYSGPVAQARAPHTPIGSAPLPEL
EECHHHHCCCCCHHHCEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHH
ASAYAPKAAARPLPLMPDLDAVPLKQTQTPIKPAVVLKPAPVAHRMAHHAPVSPPPAHAA
HHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCC
ATVKPPVVKAKVVSVKPVVLLKTAAPLPPKTAEKPKTAGKPAAKVSAKTATTEKTAVH
CCCCCCCEEEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCHHEECCCCCCCHHCCC
>Mature Secondary Structure 
SPFTPGPQSGLHRKGLRGHGTERPRRSLWQRFRLHRFSSRLLHGGLAIAAVATTTLTDT
CCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHH
AQAAPGLVIDLASGNVIYQSEATRPWYPASLTKLMTLYVALQAVRDHRIGFDTPLVVSAR
HHCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
AHAMPPSKMGFKPGTQVTLGNALKMLMVKSANDIAITVAEGIAGSVEAFAEDMNAQAADL
CCCCCCHHCCCCCCCCEEHHHHHHHHHEECCCCEEEEEECCCCHHHHHHHHHHCCHHHHC
GLRQSHFVNPNGLHNEQHYSSARDMALLARALYLRFPQYASLYNIGALRLGNQIIQNHNN
CCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
LLGRYPGVDGMKTGYTCTAGFNIVASAEQSGRRVIVVVLGSPNVETRTIKAAALFDRAFS
CEECCCCCCCCCCCEEEECCCEEEEECCCCCCEEEEEEECCCCCCHHEEHHHHHHHHHHH
NIDHAFVTLDNLPVPPDMLPSPYDVCRNRAQVMAEANAEIDTLMTPLQAGAFTPQPERAF
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEE
LFDADALGRAVPVASRITLVPRPVFDPQPVSVGPVAGYSGPVAQARAPHTPIGSAPLPEL
EECHHHHCCCCCHHHCEEECCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHH
ASAYAPKAAARPLPLMPDLDAVPLKQTQTPIKPAVVLKPAPVAHRMAHHAPVSPPPAHAA
HHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCC
ATVKPPVVKAKVVSVKPVVLLKTAAPLPPKTAEKPKTAGKPAAKVSAKTATTEKTAVH
CCCCCCCEEEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCHHEECCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]