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The map label for this gene is cysK [H]

Identifier: 182677212

GI number: 182677212

Start: 250848

End: 251837

Strand: Direct

Name: cysK [H]

Synonym: Bind_0213

Alternate gene names: 182677212

Gene position: 250848-251837 (Clockwise)

Preceding gene: 182677211

Following gene: 182677213

Centisome position: 6.02

GC content: 61.01

Gene sequence:

>990_bases
ATGGCACAGCCAGCCGTGAAACAACCTGATGCGCCTGTTCAGACCGCGGGACGCGGCCGCATTTATGATTCCATCACCGA
GACGATCGGCAATACGCCTCTCGTGCGGCTGCAGAAGATTGCCAAGGAAAAGGGCGTTAAGGCCAATCTGCTCGCCAAGC
TCGAATTCTTCAATCCGATCGCCAGCGTGAAGGACCGGATTGGCGTCCATATGATCGCGAGCCTGGAGGCCGCCGGTAAG
ATCGTCCCCGGCAAGACCACTTTGATCGAGCCGACCTCTGGCAATACTGGCATTGCGCTGGCTTTCGTCGCTGCCGCGCG
CGGCTACAAGCTGATTCTGGTCATGCCGGAATCCATGTCGCTCGAGCGGCGCAAGATGCTTGCCCTGCTCGGAGCCGAGC
TCGTTCTGACGCCCGCTCCCCAAGGGATGAAGGGCGCGATCGCCAAGGCCGAGGAACTGGTGCAGGCGACACCCGACGCC
GTGATTCCGCGCCAATTCGACAATCCCGCCAATCCTGAAATCCATCGCCTGACCACGGCCGAGGAAATCTGGAACGATAC
GGAAGGCAATGTGGATTATTTCGTCTCCGGTGTCGGCACCGGCGGCACGATCACCGGCGTCGCTCAGGTTCTGAAGCCCC
GCCGCCCGAGCCTGAAAATCGTCGCTGTCGAACCTGAGGATTCGCCCGTCCTGTCCGGCGGTCAGCCAGGTCCGCATAAG
ATCCAGGGTATCGGTGCTGGCTTCGTGCCGTCCGTTCTCGACCGGCCCCTGATCGATGAAGTCGTCACCGTCGGCAATCA
GACGGCCTTTGAGACGGCTCGCCTCGTGGCGCGCCTCGAAGGCATTCCAGTCGGTATTTCCTCTGGCGCCGCAGTCGCCG
CCGCCATCGAGGTCGGCCTGCGCCCTGAAGCCGAAGGCAAGAATATCGTCCTGATCATTCCGTCTTTTGCTGAGCGCTAT
TTGTCGACGGCTTTGTTCGAAGGTCTTTGA

Upstream 100 bases:

>100_bases
GTTCGGTTTAGCCGATCGATTTCTTTCGGCTCGGCAAGAAGCAGTTTTGCGAGAAGCTTCCTCTCTTGCCCGGCCGCGAC
ACTCTCCCTGGAGCAGTAAA

Downstream 100 bases:

>100_bases
TCTTCTCTCTATTTTACGGGCTTCACGGCAAGGCCGGCGGTGATCACCGCCGGCCTTTGTCATTCAATCTCCGCAAGCAA
CCTTTCGCGACCTTTCGTTT

Product: cysteine synthase

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MAQPAVKQPDAPVQTAGRGRIYDSITETIGNTPLVRLQKIAKEKGVKANLLAKLEFFNPIASVKDRIGVHMIASLEAAGK
IVPGKTTLIEPTSGNTGIALAFVAAARGYKLILVMPESMSLERRKMLALLGAELVLTPAPQGMKGAIAKAEELVQATPDA
VIPRQFDNPANPEIHRLTTAEEIWNDTEGNVDYFVSGVGTGGTITGVAQVLKPRRPSLKIVAVEPEDSPVLSGGQPGPHK
IQGIGAGFVPSVLDRPLIDEVVTVGNQTAFETARLVARLEGIPVGISSGAAVAAAIEVGLRPEAEGKNIVLIIPSFAERY
LSTALFEGL

Sequences:

>Translated_329_residues
MAQPAVKQPDAPVQTAGRGRIYDSITETIGNTPLVRLQKIAKEKGVKANLLAKLEFFNPIASVKDRIGVHMIASLEAAGK
IVPGKTTLIEPTSGNTGIALAFVAAARGYKLILVMPESMSLERRKMLALLGAELVLTPAPQGMKGAIAKAEELVQATPDA
VIPRQFDNPANPEIHRLTTAEEIWNDTEGNVDYFVSGVGTGGTITGVAQVLKPRRPSLKIVAVEPEDSPVLSGGQPGPHK
IQGIGAGFVPSVLDRPLIDEVVTVGNQTAFETARLVARLEGIPVGISSGAAVAAAIEVGLRPEAEGKNIVLIIPSFAERY
LSTALFEGL
>Mature_328_residues
AQPAVKQPDAPVQTAGRGRIYDSITETIGNTPLVRLQKIAKEKGVKANLLAKLEFFNPIASVKDRIGVHMIASLEAAGKI
VPGKTTLIEPTSGNTGIALAFVAAARGYKLILVMPESMSLERRKMLALLGAELVLTPAPQGMKGAIAKAEELVQATPDAV
IPRQFDNPANPEIHRLTTAEEIWNDTEGNVDYFVSGVGTGGTITGVAQVLKPRRPSLKIVAVEPEDSPVLSGGQPGPHKI
QGIGAGFVPSVLDRPLIDEVVTVGNQTAFETARLVARLEGIPVGISSGAAVAAAIEVGLRPEAEGKNIVLIIPSFAERYL
STALFEGL

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=336, Percent_Identity=41.0714285714286, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI295821200, Length=336, Percent_Identity=41.0714285714286, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI4557415, Length=336, Percent_Identity=41.0714285714286, Blast_Score=239, Evalue=2e-63,
Organism=Escherichia coli, GI1788754, Length=319, Percent_Identity=55.7993730407524, Blast_Score=298, Evalue=3e-82,
Organism=Escherichia coli, GI2367138, Length=304, Percent_Identity=42.4342105263158, Blast_Score=218, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17535051, Length=303, Percent_Identity=52.4752475247525, Blast_Score=305, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI17562970, Length=300, Percent_Identity=52.6666666666667, Blast_Score=297, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI32566674, Length=295, Percent_Identity=53.2203389830509, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI115535073, Length=311, Percent_Identity=51.7684887459807, Blast_Score=286, Evalue=9e-78,
Organism=Caenorhabditis elegans, GI17561720, Length=309, Percent_Identity=51.1326860841424, Blast_Score=269, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI17534315, Length=320, Percent_Identity=42.1875, Blast_Score=204, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI32566672, Length=212, Percent_Identity=50.4716981132076, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI25147552, Length=322, Percent_Identity=40.0621118012422, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17561716, Length=121, Percent_Identity=47.9338842975207, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321594, Length=314, Percent_Identity=40.4458598726115, Blast_Score=213, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6321449, Length=329, Percent_Identity=34.9544072948328, Blast_Score=137, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24643623, Length=326, Percent_Identity=38.9570552147239, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI20129101, Length=326, Percent_Identity=38.9570552147239, Blast_Score=197, Evalue=1e-50,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34595; Mature: 34463

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPAVKQPDAPVQTAGRGRIYDSITETIGNTPLVRLQKIAKEKGVKANLLAKLEFFNPI
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHCHH
ASVKDRIGVHMIASLEAAGKIVPGKTTLIEPTSGNTGIALAFVAAARGYKLILVMPESMS
HHHHHHHCCHHEEEHHHCCCCCCCCCEEEECCCCCCCCHHHEEHHHCCCEEEEECCCCCC
LERRKMLALLGAELVLTPAPQGMKGAIAKAEELVQATPDAVIPRQFDNPANPEIHRLTTA
HHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECH
EEIWNDTEGNVDYFVSGVGTGGTITGVAQVLKPRRPSLKIVAVEPEDSPVLSGGQPGPHK
HHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCE
IQGIGAGFVPSVLDRPLIDEVVTVGNQTAFETARLVARLEGIPVGISSGAAVAAAIEVGL
EEECCCCCCHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEHHHHCC
RPEAEGKNIVLIIPSFAERYLSTALFEGL
CCCCCCCEEEEEECHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AQPAVKQPDAPVQTAGRGRIYDSITETIGNTPLVRLQKIAKEKGVKANLLAKLEFFNPI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHCHH
ASVKDRIGVHMIASLEAAGKIVPGKTTLIEPTSGNTGIALAFVAAARGYKLILVMPESMS
HHHHHHHCCHHEEEHHHCCCCCCCCCEEEECCCCCCCCHHHEEHHHCCCEEEEECCCCCC
LERRKMLALLGAELVLTPAPQGMKGAIAKAEELVQATPDAVIPRQFDNPANPEIHRLTTA
HHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECH
EEIWNDTEGNVDYFVSGVGTGGTITGVAQVLKPRRPSLKIVAVEPEDSPVLSGGQPGPHK
HHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCE
IQGIGAGFVPSVLDRPLIDEVVTVGNQTAFETARLVARLEGIPVGISSGAAVAAAIEVGL
EEECCCCCCHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEHHHHCC
RPEAEGKNIVLIIPSFAERYLSTALFEGL
CCCCCCCEEEEEECHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]