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The map label for this gene is trmE

Identifier: 182682729

GI number: 182682729

Start: 2529009

End: 2530364

Strand: Reverse

Name: trmE

Synonym: XfasM23_2222

Alternate gene names: 182682729

Gene position: 2530364-2529009 (Counterclockwise)

Preceding gene: 182682730

Following gene: 182682728

Centisome position: 99.79

GC content: 48.75

Gene sequence:

>1356_bases
ATGAGCCAGCGCTCAACCAAGATGGGCGACACCATTGCTGCCATTGCGACTGCCTCTGGTGCTGCTGGAATAGGGATCAT
TCGTATTTCTGGCTCACAGATTAAGACAATTGCAACTGGTCTAGGAATGACCACCTTACGGCCACGATATGCGCATTACA
CCCGTTTTCTTGATGTCGATGATCAAGTCATTGATGACGGATTAGCAATATGGTTTCCTGCTCCGCATAGTTTCACTGGC
GAAGATGTACTAGAACTGCAAGGCCACGGTAGCCCACTGTTGCTACGCCAACTACTGACACGATGCCTGGATCTGGGAGC
GCGCCAAGCACGCCCTGGTGAATTTAGCGAACGTGCCTTCCTCAACGGAAAACTCGATTTGATACAAGCAGAAGCAATTG
CCGATATGATCGGCGCTGCTGACCTGCGTGCTGCCCGTGCTGCTCGTAGATCGTTGGATGGCGTATTTTCCCGGCGTTGC
GAAGCATTAGCCCAACAATTAGTCAGATTACGTATTCACGTTGAGGCAACAATCGACTTTGCCGATGAATCATTAGATAC
CCTAGACCGTGCCCAGATACGTACTTCTTTACAAACCTTGAATGTTGAATTGACCCAACTACTTCGAGACGCTGAGCATG
GCAAGCGCCTTTGTGATGGACTGTATACCGTTTTAGTTGGTCCACCGAATGTTGGTAAAAGCTCACTACTCAATGCCTTA
ATTGGTAGTGACCGTGCGATCGTCACCGATGTTCCTGGTACCACTCGTGACACATTACGAGAGAGCGTACATTTCCATGG
CTTGGAATTTGTTTTAGTTGACACTGCTGGCTTACGTGGAGAAGGCGATGCAATTGAGCGTGAAGGCATGCGTCGTACCC
TCAATGAATTACAACGCGCTGATTTAGCATTGGTCGTCTTAGATGCCTGCGATCCCCAGATTGGCAGCCTTGCCCTTGCA
GATGCCCTTACTTCAGTGCCACGCGTCCTCTGGATTCATAACAAACTTGATTTACTTACCGAGCCACCGTCCGTATTGGA
TACAGATGTGATCCCAGTGTCTGCAATGACAGGTGCTGGCTTGGACACATTGAAAACCCGCTTGCGCACATTACTGCTTG
GCGAGACAGTCGAAACAATCGAAGGTGAATTTTCGGCACGATTACGTCATGTACAAGCCTTACAACGTACAGCGGCCCAT
GTAACCGATGCGAATGCACAATTCTCCTACGAACATTTGGAATTGACAGCTGAAGAACTTCGTTTGGCTTATAAAGCACT
GGGTGAAATCAATGGCAGCATGAGCCCCGATGAATTACTCGGCCGGATCTTCTCAAACTTCTGTATTGGAAAATAA

Upstream 100 bases:

>100_bases
TAAATAATAAGGATAAAGCGCGAAAAACGTATACCACTGACGTGGATCTTCAACCAGAAGATTCTGCTACAAAGCAGCTG
GGTATGAAACTGACTAAATT

Downstream 100 bases:

>100_bases
ACACTATTTTTTAGACATCCAAACAAACCAGAAAAACCTATAAAGCCCGCTTTAATGCGGGCTTTACGCTTGACTATTAC
ACGCCCAAACATACACATCT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MSQRSTKMGDTIAAIATASGAAGIGIIRISGSQIKTIATGLGMTTLRPRYAHYTRFLDVDDQVIDDGLAIWFPAPHSFTG
EDVLELQGHGSPLLLRQLLTRCLDLGARQARPGEFSERAFLNGKLDLIQAEAIADMIGAADLRAARAARRSLDGVFSRRC
EALAQQLVRLRIHVEATIDFADESLDTLDRAQIRTSLQTLNVELTQLLRDAEHGKRLCDGLYTVLVGPPNVGKSSLLNAL
IGSDRAIVTDVPGTTRDTLRESVHFHGLEFVLVDTAGLRGEGDAIEREGMRRTLNELQRADLALVVLDACDPQIGSLALA
DALTSVPRVLWIHNKLDLLTEPPSVLDTDVIPVSAMTGAGLDTLKTRLRTLLLGETVETIEGEFSARLRHVQALQRTAAH
VTDANAQFSYEHLELTAEELRLAYKALGEINGSMSPDELLGRIFSNFCIGK

Sequences:

>Translated_451_residues
MSQRSTKMGDTIAAIATASGAAGIGIIRISGSQIKTIATGLGMTTLRPRYAHYTRFLDVDDQVIDDGLAIWFPAPHSFTG
EDVLELQGHGSPLLLRQLLTRCLDLGARQARPGEFSERAFLNGKLDLIQAEAIADMIGAADLRAARAARRSLDGVFSRRC
EALAQQLVRLRIHVEATIDFADESLDTLDRAQIRTSLQTLNVELTQLLRDAEHGKRLCDGLYTVLVGPPNVGKSSLLNAL
IGSDRAIVTDVPGTTRDTLRESVHFHGLEFVLVDTAGLRGEGDAIEREGMRRTLNELQRADLALVVLDACDPQIGSLALA
DALTSVPRVLWIHNKLDLLTEPPSVLDTDVIPVSAMTGAGLDTLKTRLRTLLLGETVETIEGEFSARLRHVQALQRTAAH
VTDANAQFSYEHLELTAEELRLAYKALGEINGSMSPDELLGRIFSNFCIGK
>Mature_450_residues
SQRSTKMGDTIAAIATASGAAGIGIIRISGSQIKTIATGLGMTTLRPRYAHYTRFLDVDDQVIDDGLAIWFPAPHSFTGE
DVLELQGHGSPLLLRQLLTRCLDLGARQARPGEFSERAFLNGKLDLIQAEAIADMIGAADLRAARAARRSLDGVFSRRCE
ALAQQLVRLRIHVEATIDFADESLDTLDRAQIRTSLQTLNVELTQLLRDAEHGKRLCDGLYTVLVGPPNVGKSSLLNALI
GSDRAIVTDVPGTTRDTLRESVHFHGLEFVLVDTAGLRGEGDAIEREGMRRTLNELQRADLALVVLDACDPQIGSLALAD
ALTSVPRVLWIHNKLDLLTEPPSVLDTDVIPVSAMTGAGLDTLKTRLRTLLLGETVETIEGEFSARLRHVQALQRTAAHV
TDANAQFSYEHLELTAEELRLAYKALGEINGSMSPDELLGRIFSNFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=469, Percent_Identity=36.6737739872068, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI193082993, Length=458, Percent_Identity=36.4628820960699, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI193082988, Length=503, Percent_Identity=33.9960238568588, Blast_Score=213, Evalue=2e-55,
Organism=Escherichia coli, GI2367268, Length=453, Percent_Identity=47.682119205298, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI87082120, Length=88, Percent_Identity=44.3181818181818, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17507259, Length=460, Percent_Identity=33.0434782608696, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323665, Length=502, Percent_Identity=29.8804780876494, Blast_Score=186, Evalue=5e-48,
Organism=Drosophila melanogaster, GI45550813, Length=481, Percent_Identity=33.4719334719335, Blast_Score=212, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49041; Mature: 48909

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRSTKMGDTIAAIATASGAAGIGIIRISGSQIKTIATGLGMTTLRPRYAHYTRFLDVD
CCCCCCCCCCHHHHHEECCCCCCEEEEEECCCHHHHHHHHCCCHHCCCHHHHHHHEECCC
DQVIDDGLAIWFPAPHSFTGEDVLELQGHGSPLLLRQLLTRCLDLGARQARPGEFSERAF
HHHHHCCEEEEECCCCCCCCCCHHEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
LNGKLDLIQAEAIADMIGAADLRAARAARRSLDGVFSRRCEALAQQLVRLRIHVEATIDF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEH
ADESLDTLDRAQIRTSLQTLNVELTQLLRDAEHGKRLCDGLYTVLVGPPNVGKSSLLNAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHH
IGSDRAIVTDVPGTTRDTLRESVHFHGLEFVLVDTAGLRGEGDAIEREGMRRTLNELQRA
HCCCCEEEECCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHC
DLALVVLDACDPQIGSLALADALTSVPRVLWIHNKLDLLTEPPSVLDTDVIPVSAMTGAG
CEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCHHCCCCCCCHHCCCCEEECHHCCCC
LDTLKTRLRTLLLGETVETIEGEFSARLRHVQALQRTAAHVTDANAQFSYEHLELTAEEL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHH
RLAYKALGEINGSMSPDELLGRIFSNFCIGK
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SQRSTKMGDTIAAIATASGAAGIGIIRISGSQIKTIATGLGMTTLRPRYAHYTRFLDVD
CCCCCCCCCHHHHHEECCCCCCEEEEEECCCHHHHHHHHCCCHHCCCHHHHHHHEECCC
DQVIDDGLAIWFPAPHSFTGEDVLELQGHGSPLLLRQLLTRCLDLGARQARPGEFSERAF
HHHHHCCEEEEECCCCCCCCCCHHEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
LNGKLDLIQAEAIADMIGAADLRAARAARRSLDGVFSRRCEALAQQLVRLRIHVEATIDF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEH
ADESLDTLDRAQIRTSLQTLNVELTQLLRDAEHGKRLCDGLYTVLVGPPNVGKSSLLNAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHH
IGSDRAIVTDVPGTTRDTLRESVHFHGLEFVLVDTAGLRGEGDAIEREGMRRTLNELQRA
HCCCCEEEECCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHC
DLALVVLDACDPQIGSLALADALTSVPRVLWIHNKLDLLTEPPSVLDTDVIPVSAMTGAG
CEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCHHCCCCCCCHHCCCCEEECHHCCCC
LDTLKTRLRTLLLGETVETIEGEFSARLRHVQALQRTAAHVTDANAQFSYEHLELTAEEL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHH
RLAYKALGEINGSMSPDELLGRIFSNFCIGK
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]