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The map label for this gene is rhtB [H]

Identifier: 182682692

GI number: 182682692

Start: 2480016

End: 2480618

Strand: Direct

Name: rhtB [H]

Synonym: XfasM23_2183

Alternate gene names: 182682692

Gene position: 2480016-2480618 (Clockwise)

Preceding gene: 182682682

Following gene: 182682694

Centisome position: 97.8

GC content: 48.42

Gene sequence:

>603_bases
ATGCATGGCCCCTCTATTTATTTCATACTGACCGTGCTGATGTTTAGCATCAGTCCGGGGCCAGCCATGATGTTTGTGCT
TCAGCAATCCCAGAAGAACGGGGTTAAAACTGCTCTGGCTGCAGTGCTTGGGACTGAGATTGGCGTATTTATTTACGTCA
TCCTCACCGCATTGGGTATCAGTACTGTTTTAAAGCAATACCCATCCATTTATACCGGCCTGCAAGGGATAGGTGCGGCC
TATCTGCTTTACATCGCCTACCTCAGCTGGCCGCGTCAGAATGCTTCAAACCAGACTCCTACAGCGTCCCGTTCAAGTTA
CACAGGCACCTTCATACAAGGAGTGCTGATTAACTTGACGAATCCCAAGATCGCTTTGTTCTTCCTGAGTCTGATCCCTC
AATTCGTCCCACGAGATAGCAATGCCATGACCTTTATGGTGTACGGGTTGATCTTCAACACGAGCGGCCTCTTAGTGAAC
TTCTCTGTCGCTCTTCTGGCAGACAGGGTCAATCGTATGCTTCGGAACGTCACATGGTTTAACTACGTGCCGCCGATTCT
CTTTATTAGCATTGCTGTTCTCTCAATGAAAGCACGTCTCTAA

Upstream 100 bases:

>100_bases
GTGGGCGATTGCATCCAGCGCGCGCGCTATTGATCTTCATTGATGCTACAAACCTATTTTTTTGAATTTTCATCATCACT
TTTTGCAAGAGAACGCAGCC

Downstream 100 bases:

>100_bases
TGCACCTGTGTCATGCGCTTCATGCCTGAAATATCCAACTGACTACGACGATTGCACAGTCCCCCTTCTCCAGAAAGGCA
TCGCACTGTGTGTATTTCAG

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MHGPSIYFILTVLMFSISPGPAMMFVLQQSQKNGVKTALAAVLGTEIGVFIYVILTALGISTVLKQYPSIYTGLQGIGAA
YLLYIAYLSWPRQNASNQTPTASRSSYTGTFIQGVLINLTNPKIALFFLSLIPQFVPRDSNAMTFMVYGLIFNTSGLLVN
FSVALLADRVNRMLRNVTWFNYVPPILFISIAVLSMKARL

Sequences:

>Translated_200_residues
MHGPSIYFILTVLMFSISPGPAMMFVLQQSQKNGVKTALAAVLGTEIGVFIYVILTALGISTVLKQYPSIYTGLQGIGAA
YLLYIAYLSWPRQNASNQTPTASRSSYTGTFIQGVLINLTNPKIALFFLSLIPQFVPRDSNAMTFMVYGLIFNTSGLLVN
FSVALLADRVNRMLRNVTWFNYVPPILFISIAVLSMKARL
>Mature_200_residues
MHGPSIYFILTVLMFSISPGPAMMFVLQQSQKNGVKTALAAVLGTEIGVFIYVILTALGISTVLKQYPSIYTGLQGIGAA
YLLYIAYLSWPRQNASNQTPTASRSSYTGTFIQGVLINLTNPKIALFFLSLIPQFVPRDSNAMTFMVYGLIFNTSGLLVN
FSVALLADRVNRMLRNVTWFNYVPPILFISIAVLSMKARL

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=164, Percent_Identity=29.8780487804878, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI1788099, Length=125, Percent_Identity=32, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI48994978, Length=138, Percent_Identity=28.9855072463768, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22032; Mature: 22032

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGPSIYFILTVLMFSISPGPAMMFVLQQSQKNGVKTALAAVLGTEIGVFIYVILTALGI
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
STVLKQYPSIYTGLQGIGAAYLLYIAYLSWPRQNASNQTPTASRSSYTGTFIQGVLINLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHEECC
NPKIALFFLSLIPQFVPRDSNAMTFMVYGLIFNTSGLLVNFSVALLADRVNRMLRNVTWF
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHH
NYVPPILFISIAVLSMKARL
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHGPSIYFILTVLMFSISPGPAMMFVLQQSQKNGVKTALAAVLGTEIGVFIYVILTALGI
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
STVLKQYPSIYTGLQGIGAAYLLYIAYLSWPRQNASNQTPTASRSSYTGTFIQGVLINLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHEECC
NPKIALFFLSLIPQFVPRDSNAMTFMVYGLIFNTSGLLVNFSVALLADRVNRMLRNVTWF
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHH
NYVPPILFISIAVLSMKARL
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]