Click here to switch to the map view.

The map label for this gene is gltD

Identifier: 182682675

GI number: 182682675

Start: 2450342

End: 2451883

Strand: Reverse

Name: gltD

Synonym: XfasM23_2165

Alternate gene names: 182682675

Gene position: 2451883-2450342 (Counterclockwise)

Preceding gene: 182682676

Following gene: 182682673

Centisome position: 96.69

GC content: 56.49

Gene sequence:

>1542_bases
ATGTGTTCCCGCTGGATGGTGAGTGCGTGGAACGAAGAAGTCACGGAATCTTTATTTATGAGTCGCAAGCACGCTTTCCA
ATTTCTTGATTTGCCTCGGCAGATGCCGCAACGCATCCCGGTGGAGCTGCGTACTTCCGGGGATTGGCGGGAGTTATACG
GTAAGTTCGATAAGGGTGAAGCGCAGTACCAAGCCGGTCGCTGTTTGGACTGTGGTAATCCATATTGCAGTTGGAAGTGT
CCGGTGCATAACGCCATCCCGCAGTGGTTGCAGTTAGTGCAGGAAAATCGCATTGATGAGGCCGCAGCGTTGTGCCATGC
AACCAACCCGCTTCCTGAAGTGTGCGGCAGGGTGTGTCCGCAGGACCGTCTCTGTGAAGGCAGTTGCACGCTGGAGGAGT
TTGGTGCGGTCACTATCGGTGCGGTGGAAAAGTACATTGTGGATACCGCGTTGCAGCAGGGGTGGCGTCCCGATTTGAGC
CATGTGCAACCGACGGGTTGGCGTGTGGCGGTGGTCGGTGCAGGGCCTGCCGGCCTTGCCTGTGCGGATCGTTTGGCGCG
TGCTGGGGTCCAGGCGGTGGTGTATGACCGCTATGAGCAGATCGGCGGGTTGCTGCAGTTCGGGATTCCCAGTTTTAAGC
TTGATAAGGCGGTGATGAGTACGCGCCGCCAAGTGCTTGAGGGCATGGGCGTAGAGTTTCGATTGGGTGTGGAGATTGGA
AAGGATCTGGGGATTGAAGCGTTGTTGGAGGACTACGATGCTGTGTTCCTTGGCGTCGGTGCGTATCGCTACACGGACGG
TGGCTTGCCGGGTCAGGACTTGAAAAACGTGTTGCCGGCATTACCGTTTTTGGTTCAGAACGGACGTATCATTTCTGGCA
ATGATCCGTATGGCCGTCCGATTGCCGGTTGGGAGGATCAGGTGCAGTTGCCGGATGTGGCGGGGAAGTCTGTGGTTGTA
CTCGGTGGCGGTGATACCGGCATGGATTGTGTACGGAGTGCGATCCGTTTAGGTGCCACCAAAGTCACCTGTATCTATCG
GCGTGATGAGGCGAATATGCCTGGCTCGGCGCGTGAGGTTGCTAACGCGCGGGAAGAGGGGGTGCGCTTTCTGTTTAATC
GTCAGCCATTGTCGATTCAATCCGGTACCGACAAACAAGCCATTGGTGTCACTGTGGTGGAAACGAAGCTTGCTGAGCCG
GATGCCAATGGTCGTCGCAACGCAGTGCCTATCCAGGGTACTGAAGCGGTGCTGGAAGCGGATGTGGTGATTATTGCTTT
TGGTTTTTCGCCGATGTTGCTGGAGTGGTTAACTGCGCAGGGTGTACAAGCGTCGAGCAACGGCCGCATTGTGGTGGCGT
TGTCTGATACTGGCGATAGACTGACCTATCAGACAACGCATCCGAAGTTGTTTGCTGGTGGTGATTGTGTGCGTGGTGCC
GATTTGGTTGTCACTGCCGTGGCCGAGGGGCGCGACGCGGCTGAGAGCATTGTGCGGTGGTTGCGTGCGACTGCACAGGT
GATGGATGTTGCGGTGATCTGA

Upstream 100 bases:

>100_bases
CTACGCAGTGCCGCTTAATGTGTGTGTTTCCCATTGCATAAACTAGAGAGAAGCAGGAAGGGACTGCGTTTTTCTCTCTG
CTATCGCTCTTTCTCAGAGT

Downstream 100 bases:

>100_bases
GCGGGATGCTGACGCATTGCACCAAGCCTAGTGGGCTTCGCTGGAGGGAGGATGAATATTGGTTCTCCTTTTGGTTCTCC
TTGAGTTTATCTTTGGGATG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MCSRWMVSAWNEEVTESLFMSRKHAFQFLDLPRQMPQRIPVELRTSGDWRELYGKFDKGEAQYQAGRCLDCGNPYCSWKC
PVHNAIPQWLQLVQENRIDEAAALCHATNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTIGAVEKYIVDTALQQGWRPDLS
HVQPTGWRVAVVGAGPAGLACADRLARAGVQAVVYDRYEQIGGLLQFGIPSFKLDKAVMSTRRQVLEGMGVEFRLGVEIG
KDLGIEALLEDYDAVFLGVGAYRYTDGGLPGQDLKNVLPALPFLVQNGRIISGNDPYGRPIAGWEDQVQLPDVAGKSVVV
LGGGDTGMDCVRSAIRLGATKVTCIYRRDEANMPGSAREVANAREEGVRFLFNRQPLSIQSGTDKQAIGVTVVETKLAEP
DANGRRNAVPIQGTEAVLEADVVIIAFGFSPMLLEWLTAQGVQASSNGRIVVALSDTGDRLTYQTTHPKLFAGGDCVRGA
DLVVTAVAEGRDAAESIVRWLRATAQVMDVAVI

Sequences:

>Translated_513_residues
MCSRWMVSAWNEEVTESLFMSRKHAFQFLDLPRQMPQRIPVELRTSGDWRELYGKFDKGEAQYQAGRCLDCGNPYCSWKC
PVHNAIPQWLQLVQENRIDEAAALCHATNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTIGAVEKYIVDTALQQGWRPDLS
HVQPTGWRVAVVGAGPAGLACADRLARAGVQAVVYDRYEQIGGLLQFGIPSFKLDKAVMSTRRQVLEGMGVEFRLGVEIG
KDLGIEALLEDYDAVFLGVGAYRYTDGGLPGQDLKNVLPALPFLVQNGRIISGNDPYGRPIAGWEDQVQLPDVAGKSVVV
LGGGDTGMDCVRSAIRLGATKVTCIYRRDEANMPGSAREVANAREEGVRFLFNRQPLSIQSGTDKQAIGVTVVETKLAEP
DANGRRNAVPIQGTEAVLEADVVIIAFGFSPMLLEWLTAQGVQASSNGRIVVALSDTGDRLTYQTTHPKLFAGGDCVRGA
DLVVTAVAEGRDAAESIVRWLRATAQVMDVAVI
>Mature_513_residues
MCSRWMVSAWNEEVTESLFMSRKHAFQFLDLPRQMPQRIPVELRTSGDWRELYGKFDKGEAQYQAGRCLDCGNPYCSWKC
PVHNAIPQWLQLVQENRIDEAAALCHATNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTIGAVEKYIVDTALQQGWRPDLS
HVQPTGWRVAVVGAGPAGLACADRLARAGVQAVVYDRYEQIGGLLQFGIPSFKLDKAVMSTRRQVLEGMGVEFRLGVEIG
KDLGIEALLEDYDAVFLGVGAYRYTDGGLPGQDLKNVLPALPFLVQNGRIISGNDPYGRPIAGWEDQVQLPDVAGKSVVV
LGGGDTGMDCVRSAIRLGATKVTCIYRRDEANMPGSAREVANAREEGVRFLFNRQPLSIQSGTDKQAIGVTVVETKLAEP
DANGRRNAVPIQGTEAVLEADVVIIAFGFSPMLLEWLTAQGVQASSNGRIVVALSDTGDRLTYQTTHPKLFAGGDCVRGA
DLVVTAVAEGRDAAESIVRWLRATAQVMDVAVI

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=463, Percent_Identity=28.7257019438445, Blast_Score=152, Evalue=1e-36,
Organism=Escherichia coli, GI1789606, Length=481, Percent_Identity=56.5488565488565, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI1788811, Length=473, Percent_Identity=54.3340380549683, Blast_Score=523, Evalue=1e-149,
Organism=Escherichia coli, GI87082180, Length=488, Percent_Identity=49.7950819672131, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1788468, Length=455, Percent_Identity=29.4505494505495, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1789243, Length=439, Percent_Identity=32.8018223234624, Blast_Score=141, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17570289, Length=483, Percent_Identity=33.7474120082816, Blast_Score=228, Evalue=7e-60,
Organism=Caenorhabditis elegans, GI71984108, Length=484, Percent_Identity=30.3719008264463, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17543860, Length=318, Percent_Identity=24.8427672955975, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=477, Percent_Identity=34.3815513626834, Blast_Score=246, Evalue=6e-66,
Organism=Drosophila melanogaster, GI28574881, Length=527, Percent_Identity=35.2941176470588, Blast_Score=255, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24665539, Length=527, Percent_Identity=35.2941176470588, Blast_Score=255, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24665547, Length=528, Percent_Identity=35.2272727272727, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24665543, Length=528, Percent_Identity=35.2272727272727, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24640763, Length=448, Percent_Identity=29.6875, Blast_Score=161, Evalue=9e-40,
Organism=Drosophila melanogaster, GI18858217, Length=448, Percent_Identity=29.6875, Blast_Score=161, Evalue=9e-40,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 55798; Mature: 55798

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSRWMVSAWNEEVTESLFMSRKHAFQFLDLPRQMPQRIPVELRTSGDWRELYGKFDKGE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCEEEECCCCHHHHCCCCCCCC
AQYQAGRCLDCGNPYCSWKCPVHNAIPQWLQLVQENRIDEAAALCHATNPLPEVCGRVCP
HHHCCCCEEECCCCCCEEECCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCC
QDRLCEGSCTLEEFGAVTIGAVEKYIVDTALQQGWRPDLSHVQPTGWRVAVVGAGPAGLA
CCCCCCCCCCHHHHCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHH
CADRLARAGVQAVVYDRYEQIGGLLQFGIPSFKLDKAVMSTRRQVLEGMGVEFRLGVEIG
HHHHHHHHCHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECC
KDLGIEALLEDYDAVFLGVGAYRYTDGGLPGQDLKNVLPALPFLVQNGRIISGNDPYGRP
CCCCHHHHHHHHCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCC
IAGWEDQVQLPDVAGKSVVVLGGGDTGMDCVRSAIRLGATKVTCIYRRDEANMPGSAREV
CCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHH
ANAREEGVRFLFNRQPLSIQSGTDKQAIGVTVVETKLAEPDANGRRNAVPIQGTEAVLEA
HHHHHHHHHHHCCCCCCEECCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHC
DVVIIAFGFSPMLLEWLTAQGVQASSNGRIVVALSDTGDRLTYQTTHPKLFAGGDCVRGA
CEEEEEECCHHHHHHHHHHCCCEECCCCEEEEEECCCCCEEEEEECCCEEEECCCCCCCC
DLVVTAVAEGRDAAESIVRWLRATAQVMDVAVI
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCSRWMVSAWNEEVTESLFMSRKHAFQFLDLPRQMPQRIPVELRTSGDWRELYGKFDKGE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCEEEECCCCHHHHCCCCCCCC
AQYQAGRCLDCGNPYCSWKCPVHNAIPQWLQLVQENRIDEAAALCHATNPLPEVCGRVCP
HHHCCCCEEECCCCCCEEECCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCC
QDRLCEGSCTLEEFGAVTIGAVEKYIVDTALQQGWRPDLSHVQPTGWRVAVVGAGPAGLA
CCCCCCCCCCHHHHCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHH
CADRLARAGVQAVVYDRYEQIGGLLQFGIPSFKLDKAVMSTRRQVLEGMGVEFRLGVEIG
HHHHHHHHCHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECC
KDLGIEALLEDYDAVFLGVGAYRYTDGGLPGQDLKNVLPALPFLVQNGRIISGNDPYGRP
CCCCHHHHHHHHCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCC
IAGWEDQVQLPDVAGKSVVVLGGGDTGMDCVRSAIRLGATKVTCIYRRDEANMPGSAREV
CCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHHH
ANAREEGVRFLFNRQPLSIQSGTDKQAIGVTVVETKLAEPDANGRRNAVPIQGTEAVLEA
HHHHHHHHHHHCCCCCCEECCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHC
DVVIIAFGFSPMLLEWLTAQGVQASSNGRIVVALSDTGDRLTYQTTHPKLFAGGDCVRGA
CEEEEEECCHHHHHHHHHHCCCEECCCCEEEEEECCCCCEEEEEECCCEEEECCCCCCCC
DLVVTAVAEGRDAAESIVRWLRATAQVMDVAVI
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]