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The map label for this gene is rpoH [H]

Identifier: 182682661

GI number: 182682661

Start: 2430777

End: 2431643

Strand: Reverse

Name: rpoH [H]

Synonym: XfasM23_2151

Alternate gene names: 182682661

Gene position: 2431643-2430777 (Counterclockwise)

Preceding gene: 182682662

Following gene: 182682660

Centisome position: 95.9

GC content: 47.29

Gene sequence:

>867_bases
ATGACTCGAAATACATCAATGGTGCTTGTGGGAAACAACTTTCCTGTTCCAAATGCCCTCGGTTCGTTGGAGGCATACAT
CAGTGCCGTGCACCAGATATCGGTACTTTCGGTTGATGAGGAGCAGCTATTGGCTCGCCGTTATCGTGATCAACAGGATT
TAGATGCTGCACGTGAGTTGGTGCATTCGCACTTGCGTTTTGTTGTTCGGGTAGCGCGCGGTTATAGCGGTTACGGCCTG
TCTTTAGGCGACTTAATACAAGAGGGTAATATCGGTCTGATGAAAGCTGTAAAGCGCTTTGATCCGGATATGGGTGTCCG
TTTGGTTTCTTTTGCAATGCATTGGATTCGCGCTGAAATGCATGAGTTTATTTTGAAGAATTGGCGCATCGTTAAAGTTG
CTACCACCAAAGCGCAGCGAAAGCTGTTTTTTAACCTTCGGAAATCTAGAACCCGTCTGGGCTGGATGAATGCCGAGGAA
GTTAGTGCAGTTGCTAGGGATCTTAACGTCTCCGAACGTGAGGTATTGGAGATGGAGTCACGTTTGTCAGGGCGCGATAT
TGGTTTCGACGCGCCGGAGGAAGACAACGCTCCGCCATCGCCTGTTGGCTACTTGATTGCTAATGATGGAGATCCTTCTC
AAGCTTACGAACGTGGTAATAGTGAGGAAACCCAATTACGCCTATTGCAGGAAGGTTTGTCTGTCCTTGATACCCGTTCG
CGCGATATTATTCAGCGTCGCTGGCTGGAAACAGATAAGAAAATCACGTTGCAAGAATTAGCTGAAGAATATGGAGTTTC
AGCAGAACGTATTCGGCAGATTGAAGCTAATGCGCTTAAAAAAATGAAGGTGCTGTTCGCTGCTTGA

Upstream 100 bases:

>100_bases
GGATGCCATTTTAATTTAAATGGAGTTTTTCTAAAGATTTGTTCATTTTAAACGTATAGCTGTCACTGTGTGGTTACTTT
AAGATTTGGGATACTTGAGT

Downstream 100 bases:

>100_bases
ATGCTCTGTTTCAAAACAAAGGCTACAAAGTGGCTAGTGGTTGTTGCTTGTATGTATTTTTCTCGTTTTTGAAGATGCTT
GGATCTTCCTGATGCTTAGT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MTRNTSMVLVGNNFPVPNALGSLEAYISAVHQISVLSVDEEQLLARRYRDQQDLDAARELVHSHLRFVVRVARGYSGYGL
SLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAMHWIRAEMHEFILKNWRIVKVATTKAQRKLFFNLRKSRTRLGWMNAEE
VSAVARDLNVSEREVLEMESRLSGRDIGFDAPEEDNAPPSPVGYLIANDGDPSQAYERGNSEETQLRLLQEGLSVLDTRS
RDIIQRRWLETDKKITLQELAEEYGVSAERIRQIEANALKKMKVLFAA

Sequences:

>Translated_288_residues
MTRNTSMVLVGNNFPVPNALGSLEAYISAVHQISVLSVDEEQLLARRYRDQQDLDAARELVHSHLRFVVRVARGYSGYGL
SLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAMHWIRAEMHEFILKNWRIVKVATTKAQRKLFFNLRKSRTRLGWMNAEE
VSAVARDLNVSEREVLEMESRLSGRDIGFDAPEEDNAPPSPVGYLIANDGDPSQAYERGNSEETQLRLLQEGLSVLDTRS
RDIIQRRWLETDKKITLQELAEEYGVSAERIRQIEANALKKMKVLFAA
>Mature_287_residues
TRNTSMVLVGNNFPVPNALGSLEAYISAVHQISVLSVDEEQLLARRYRDQQDLDAARELVHSHLRFVVRVARGYSGYGLS
LGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAMHWIRAEMHEFILKNWRIVKVATTKAQRKLFFNLRKSRTRLGWMNAEEV
SAVARDLNVSEREVLEMESRLSGRDIGFDAPEEDNAPPSPVGYLIANDGDPSQAYERGNSEETQLRLLQEGLSVLDTRSR
DIIQRRWLETDKKITLQELAEEYGVSAERIRQIEANALKKMKVLFAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=272, Percent_Identity=55.5147058823529, Blast_Score=302, Evalue=2e-83,
Organism=Escherichia coli, GI1789098, Length=267, Percent_Identity=28.0898876404494, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1789448, Length=234, Percent_Identity=30.7692307692308, Blast_Score=88, Evalue=8e-19,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32759; Mature: 32628

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRNTSMVLVGNNFPVPNALGSLEAYISAVHQISVLSVDEEQLLARRYRDQQDLDAAREL
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHH
VHSHLRFVVRVARGYSGYGLSLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAMHWIRAEM
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
HEFILKNWRIVKVATTKAQRKLFFNLRKSRTRLGWMNAEEVSAVARDLNVSEREVLEMES
HHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHH
RLSGRDIGFDAPEEDNAPPSPVGYLIANDGDPSQAYERGNSEETQLRLLQEGLSVLDTRS
HCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCH
RDIIQRRWLETDKKITLQELAEEYGVSAERIRQIEANALKKMKVLFAA
HHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRNTSMVLVGNNFPVPNALGSLEAYISAVHQISVLSVDEEQLLARRYRDQQDLDAAREL
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHH
VHSHLRFVVRVARGYSGYGLSLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAMHWIRAEM
HHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
HEFILKNWRIVKVATTKAQRKLFFNLRKSRTRLGWMNAEEVSAVARDLNVSEREVLEMES
HHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHH
RLSGRDIGFDAPEEDNAPPSPVGYLIANDGDPSQAYERGNSEETQLRLLQEGLSVLDTRS
HCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCH
RDIIQRRWLETDKKITLQELAEEYGVSAERIRQIEANALKKMKVLFAA
HHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]