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The map label for this gene is pilR [H]

Identifier: 182682547

GI number: 182682547

Start: 2277462

End: 2278943

Strand: Reverse

Name: pilR [H]

Synonym: XfasM23_2035

Alternate gene names: 182682547

Gene position: 2278943-2277462 (Counterclockwise)

Preceding gene: 182682548

Following gene: 182682544

Centisome position: 89.87

GC content: 52.43

Gene sequence:

>1482_bases
ATGCGTTGCCGTGCGTCTGAAGACGATGGGAATTGTCCATCTTCAGTCTGCTGCGTTATCTTGGCATTCATGAATGAATC
CCGCAGTGCTCTGATTGTCGATGACGAGCGCGACATCCGTGAACTTTTGGTCCTTACGCTAGGACGTATGGGGCTGCGTA
TCAGAACGGCAGCTAATCTGGCACAAGCATACGCGTTACTTGAGTCTGACTCGTTCAATCTTTGTATCACCGACATGCGT
TTACCTGACGGCAGTGGCATTGAGCTGGTGAAAAAGATTGTGCGTCATTATCCACGCACCCCGGTGGCCATGATCACTGC
TTTTGGCAGTATCGAACTGGCGGTAGAGGCGCTCAAGGAAGGTGCTTTTGACTTTGTCAGCAAACCTGTTGATCTGAACG
TCCTGCGAGGCATGGTCCGTCATGCTTTGGAATTGAACAATCGTGAACGCTCTGTTGTGTCAAGTCAGCCACAAAGCCCC
AGCCAACGTCTCCTTGGTGAATCATCACCGATGGCTCAGCTTCGGGCCACCATCGCTAAAGTGGCGCGTAGTCAAGCCCC
GGTTTATATCCTTGGTGAATCAGGAGTTGGTAAAGAACTGGTTGCACGTACGATCCACGAACAGAGTGTGCGTGCTGCTG
GGGCGTTCGTGCCGGTCAACTGTGGTGCGATCCCTGTGGATCTTATGGAAAGCGAATTTTTCGGTCACAAGAAAGGGAGT
TTCACCGGTGCGCATACTGATAAGCCAGGTTTATTCCAAAGTGCTGGCGGCGGTACTTTATTCCTGGATGAAGTTGCCGA
ATTACCCTTGCACATGCAAGTTAAATTGCTGCGTGCGATTCAGGAGAAATCGATACGGCCAATCGGTGCAACCGTTGAGG
TACCAGTAGATGTGCGTATTTTATCGGCGACTCACCAAGATCTGAGTGACCTGATTGCCAATGGCCGCTTCCGTCACGAT
TTGTACTACCGCATTAATGTAATCGAGATACGTGTTCCACCGCTACGTGAGCGTGCTGGCGATTTGCCGCATCTTGCTGC
AGCCATTGTGGCTCGCTTGGCACACACGCATGGGCGGGCGGTCCCGTTGCTTTCACCCTCCGCCTTGGAGGCGTTAGAAC
ATTATTCGTTCCCGGGTAATGTGCGTGAACTGGAGAACATTCTGGAGCGTGCTCTAGCACTGACTGAAGATGATCATATC
GGTGCCGCGGATCTGCACCTGCCCCAGGGCAGTTTGCATGGACAGGGATTGCCGCTCCCGTTAGTGCCCACAGTTCTCCC
GATTGTGCCTGCGGACGCATCAGCATCGGTGGAGGGTGAGAATACGCCGGCTGCCTTGCCCTCGTACATGGAGCAGCTGG
AACGTGCTGCCATTCAAAAGGCGTTGGAAGAGAATCGCTGGAATAAAACAAAGACAGCCGCAAAACTTGGCATCACTTTC
CGTGCATTACGTTACAAACTAAAGAAGCTGGGTATGGAGTGA

Upstream 100 bases:

>100_bases
TTCACATTCTTTCGCATTTAGAGAGAGCGTGGCCGTTGCGGCGTGTTAGCGTCGCTATTGCAGATTGCAACCCTATGGGC
GGTTAATATTGGCATCTTCG

Downstream 100 bases:

>100_bases
TGAGAGGAAGCAGGCAACATAGCCTGCCTTGCTTCTTCTCAGCATGGTGTCATGACGTGCTTTGATTCAGTCATTAACAG
GTGTTGTTGATGCCTAATCT

Product: two component sigma-54 specific Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MRCRASEDDGNCPSSVCCVILAFMNESRSALIVDDERDIRELLVLTLGRMGLRIRTAANLAQAYALLESDSFNLCITDMR
LPDGSGIELVKKIVRHYPRTPVAMITAFGSIELAVEALKEGAFDFVSKPVDLNVLRGMVRHALELNNRERSVVSSQPQSP
SQRLLGESSPMAQLRATIAKVARSQAPVYILGESGVGKELVARTIHEQSVRAAGAFVPVNCGAIPVDLMESEFFGHKKGS
FTGAHTDKPGLFQSAGGGTLFLDEVAELPLHMQVKLLRAIQEKSIRPIGATVEVPVDVRILSATHQDLSDLIANGRFRHD
LYYRINVIEIRVPPLRERAGDLPHLAAAIVARLAHTHGRAVPLLSPSALEALEHYSFPGNVRELENILERALALTEDDHI
GAADLHLPQGSLHGQGLPLPLVPTVLPIVPADASASVEGENTPAALPSYMEQLERAAIQKALEENRWNKTKTAAKLGITF
RALRYKLKKLGME

Sequences:

>Translated_493_residues
MRCRASEDDGNCPSSVCCVILAFMNESRSALIVDDERDIRELLVLTLGRMGLRIRTAANLAQAYALLESDSFNLCITDMR
LPDGSGIELVKKIVRHYPRTPVAMITAFGSIELAVEALKEGAFDFVSKPVDLNVLRGMVRHALELNNRERSVVSSQPQSP
SQRLLGESSPMAQLRATIAKVARSQAPVYILGESGVGKELVARTIHEQSVRAAGAFVPVNCGAIPVDLMESEFFGHKKGS
FTGAHTDKPGLFQSAGGGTLFLDEVAELPLHMQVKLLRAIQEKSIRPIGATVEVPVDVRILSATHQDLSDLIANGRFRHD
LYYRINVIEIRVPPLRERAGDLPHLAAAIVARLAHTHGRAVPLLSPSALEALEHYSFPGNVRELENILERALALTEDDHI
GAADLHLPQGSLHGQGLPLPLVPTVLPIVPADASASVEGENTPAALPSYMEQLERAAIQKALEENRWNKTKTAAKLGITF
RALRYKLKKLGME
>Mature_493_residues
MRCRASEDDGNCPSSVCCVILAFMNESRSALIVDDERDIRELLVLTLGRMGLRIRTAANLAQAYALLESDSFNLCITDMR
LPDGSGIELVKKIVRHYPRTPVAMITAFGSIELAVEALKEGAFDFVSKPVDLNVLRGMVRHALELNNRERSVVSSQPQSP
SQRLLGESSPMAQLRATIAKVARSQAPVYILGESGVGKELVARTIHEQSVRAAGAFVPVNCGAIPVDLMESEFFGHKKGS
FTGAHTDKPGLFQSAGGGTLFLDEVAELPLHMQVKLLRAIQEKSIRPIGATVEVPVDVRILSATHQDLSDLIANGRFRHD
LYYRINVIEIRVPPLRERAGDLPHLAAAIVARLAHTHGRAVPLLSPSALEALEHYSFPGNVRELENILERALALTEDDHI
GAADLHLPQGSLHGQGLPLPLVPTVLPIVPADASASVEGENTPAALPSYMEQLERAAIQKALEENRWNKTKTAAKLGITF
RALRYKLKKLGME

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=468, Percent_Identity=39.957264957265, Blast_Score=327, Evalue=9e-91,
Organism=Escherichia coli, GI1790437, Length=463, Percent_Identity=39.524838012959, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI1790299, Length=471, Percent_Identity=36.3057324840764, Blast_Score=291, Evalue=7e-80,
Organism=Escherichia coli, GI1788905, Length=460, Percent_Identity=37.6086956521739, Blast_Score=283, Evalue=3e-77,
Organism=Escherichia coli, GI1789233, Length=339, Percent_Identity=40.4129793510324, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87082117, Length=244, Percent_Identity=45.0819672131148, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1789087, Length=333, Percent_Identity=38.4384384384384, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI87082152, Length=331, Percent_Identity=41.0876132930514, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1786524, Length=393, Percent_Identity=36.3867684478371, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87081872, Length=330, Percent_Identity=35.1515151515151, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1787583, Length=360, Percent_Identity=33.3333333333333, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1789828, Length=217, Percent_Identity=41.4746543778802, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI87081858, Length=227, Percent_Identity=35.2422907488987, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1789809, Length=139, Percent_Identity=30.9352517985612, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53732; Mature: 53732

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCRASEDDGNCPSSVCCVILAFMNESRSALIVDDERDIRELLVLTLGRMGLRIRTAANL
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCEEEHHHHHH
AQAYALLESDSFNLCITDMRLPDGSGIELVKKIVRHYPRTPVAMITAFGSIELAVEALKE
HHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
GAFDFVSKPVDLNVLRGMVRHALELNNRERSVVSSQPQSPSQRLLGESSPMAQLRATIAK
CHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHH
VARSQAPVYILGESGVGKELVARTIHEQSVRAAGAFVPVNCGAIPVDLMESEFFGHKKGS
HHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCCCC
FTGAHTDKPGLFQSAGGGTLFLDEVAELPLHMQVKLLRAIQEKSIRPIGATVEVPVDVRI
CCCCCCCCCCCEECCCCCEEEHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCEEE
LSATHQDLSDLIANGRFRHDLYYRINVIEIRVPPLRERAGDLPHLAAAIVARLAHTHGRA
EHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCCHHHHCCCCHHHHHHHHHHHHHHCCCC
VPLLSPSALEALEHYSFPGNVRELENILERALALTEDDHIGAADLHLPQGSLHGQGLPLP
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCC
LVPTVLPIVPADASASVEGENTPAALPSYMEQLERAAIQKALEENRWNKTKTAAKLGITF
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RALRYKLKKLGME
HHHHHHHHHHCCC
>Mature Secondary Structure
MRCRASEDDGNCPSSVCCVILAFMNESRSALIVDDERDIRELLVLTLGRMGLRIRTAANL
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCEEEHHHHHH
AQAYALLESDSFNLCITDMRLPDGSGIELVKKIVRHYPRTPVAMITAFGSIELAVEALKE
HHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
GAFDFVSKPVDLNVLRGMVRHALELNNRERSVVSSQPQSPSQRLLGESSPMAQLRATIAK
CHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHH
VARSQAPVYILGESGVGKELVARTIHEQSVRAAGAFVPVNCGAIPVDLMESEFFGHKKGS
HHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCCCC
FTGAHTDKPGLFQSAGGGTLFLDEVAELPLHMQVKLLRAIQEKSIRPIGATVEVPVDVRI
CCCCCCCCCCCEECCCCCEEEHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCEEE
LSATHQDLSDLIANGRFRHDLYYRINVIEIRVPPLRERAGDLPHLAAAIVARLAHTHGRA
EHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCCHHHHCCCCHHHHHHHHHHHHHHCCCC
VPLLSPSALEALEHYSFPGNVRELENILERALALTEDDHIGAADLHLPQGSLHGQGLPLP
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCC
LVPTVLPIVPADASASVEGENTPAALPSYMEQLERAAIQKALEENRWNKTKTAAKLGITF
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RALRYKLKKLGME
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]