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The map label for this gene is mraW

Identifier: 182682489

GI number: 182682489

Start: 2217407

End: 2218393

Strand: Reverse

Name: mraW

Synonym: XfasM23_1976

Alternate gene names: 182682489

Gene position: 2218393-2217407 (Counterclockwise)

Preceding gene: 182682490

Following gene: 182682488

Centisome position: 87.49

GC content: 55.52

Gene sequence:

>987_bases
ATGTGTGACGAGGGGTGCTTGGATGGCTTGGCACCGCACTTGTCAATGACTCATGTCCCAGTGCTCTACACCCAGGCCAT
GGAAGGGTTGCGGGTGGTTGAAAACGGAACCTATTTGGATGGCACATTCGGGCGTGGAGGACACGCACGAGGGGTGCTGC
AACAACTAGGCCCTGGAGGCCGACTGCTAGTGATGGATAAGGATCCAGAAGCGATCGCAATGGCTGAACGTGCGTTCAGC
TGTGATCCGCGCGTGGTCATTCGTCATGGCAGCTTTGCTTTGCTCGCGCAGTTGGCTGCGCCACAGTCTTTGGATGGTGT
GTTGTTTGATCTAGGAGTGTCTTCGCCGCAGTTGGATGTTCCAGAGCGTGGTTTTTCTTTTGCTAAGGATGGCCCGCTTG
ATATGCGCATGGATCCGGAGATGGGGGAGAGTGCGGCGCAGTGGCTGGCACGTGTCTCCGAGCGTGAAATCGCTGACGTA
CTTTGGACCTACGGCGAGGAAAAACAGAGCCGTCGTATTGCGCGTGCGATTGTCGCCTACCGCGCCAATCAGCCGTTGTT
ACGTACCGTGCAGTTGGCTGAATTAATTGCATCTGTGATGTTACGCACTAAGTTTGGTGCCTGTAAATCCAGGATTCATC
CTGCGACGCGCAGTTTTCAGGGAATCCGTATCCATGTGAACCGTGAGTTGGTGGATTTGGAGGTTGGTCTTGAAGCAGCA
CTCGCAGCATTGCGCCCGGGTGGGCGGTTGGTCGTGATCAGTTTTCATTCACTGGAGGACCGTATCGTCAAACAGTTTAT
CAGTCGTCATTCCAAGGTGCCGCCTACCAATCGTCGCCTACCTGAGGTGCAGACCTTCGTGCCGTTATTACGGATGATCG
GTCGTGCGATCAAGGCCGATGAGGATGAGTTGGAGGTTAACCCGCGTGCGCGCAGTGCGGTGCTTCGAGTGGCTGAGAAG
TTGGATGTGTTGGGGGCGGTGCGATGA

Upstream 100 bases:

>100_bases
CTTTGGAGCGAGCAGGCGCATCACGCATTGATCCAGCAGACACTCTCTGATGGTGATTTGGGCGATGGACTACTCGATCT
CAGATTGTGAGTGGGGACGG

Downstream 100 bases:

>100_bases
GCCGTCTGCTGTTGATTATTCTTTTGGCTTGTACGGTCGCTTCGGCGATCGGTGTGGTTTACATGCGCCATCTGCATCGA
AAGTTATTCGTTGAGCTGTC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MCDEGCLDGLAPHLSMTHVPVLYTQAMEGLRVVENGTYLDGTFGRGGHARGVLQQLGPGGRLLVMDKDPEAIAMAERAFS
CDPRVVIRHGSFALLAQLAAPQSLDGVLFDLGVSSPQLDVPERGFSFAKDGPLDMRMDPEMGESAAQWLARVSEREIADV
LWTYGEEKQSRRIARAIVAYRANQPLLRTVQLAELIASVMLRTKFGACKSRIHPATRSFQGIRIHVNRELVDLEVGLEAA
LAALRPGGRLVVISFHSLEDRIVKQFISRHSKVPPTNRRLPEVQTFVPLLRMIGRAIKADEDELEVNPRARSAVLRVAEK
LDVLGAVR

Sequences:

>Translated_328_residues
MCDEGCLDGLAPHLSMTHVPVLYTQAMEGLRVVENGTYLDGTFGRGGHARGVLQQLGPGGRLLVMDKDPEAIAMAERAFS
CDPRVVIRHGSFALLAQLAAPQSLDGVLFDLGVSSPQLDVPERGFSFAKDGPLDMRMDPEMGESAAQWLARVSEREIADV
LWTYGEEKQSRRIARAIVAYRANQPLLRTVQLAELIASVMLRTKFGACKSRIHPATRSFQGIRIHVNRELVDLEVGLEAA
LAALRPGGRLVVISFHSLEDRIVKQFISRHSKVPPTNRRLPEVQTFVPLLRMIGRAIKADEDELEVNPRARSAVLRVAEK
LDVLGAVR
>Mature_328_residues
MCDEGCLDGLAPHLSMTHVPVLYTQAMEGLRVVENGTYLDGTFGRGGHARGVLQQLGPGGRLLVMDKDPEAIAMAERAFS
CDPRVVIRHGSFALLAQLAAPQSLDGVLFDLGVSSPQLDVPERGFSFAKDGPLDMRMDPEMGESAAQWLARVSEREIADV
LWTYGEEKQSRRIARAIVAYRANQPLLRTVQLAELIASVMLRTKFGACKSRIHPATRSFQGIRIHVNRELVDLEVGLEAA
LAALRPGGRLVVISFHSLEDRIVKQFISRHSKVPPTNRRLPEVQTFVPLLRMIGRAIKADEDELEVNPRARSAVLRVAEK
LDVLGAVR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=338, Percent_Identity=39.6449704142012, Blast_Score=205, Evalue=4e-53,
Organism=Homo sapiens, GI165377202, Length=190, Percent_Identity=42.1052631578947, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1786270, Length=319, Percent_Identity=50.7836990595611, Blast_Score=292, Evalue=2e-80,
Organism=Drosophila melanogaster, GI62472493, Length=335, Percent_Identity=39.7014925373134, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI28571637, Length=335, Percent_Identity=39.7014925373134, Blast_Score=184, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 36138; Mature: 36138

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCDEGCLDGLAPHLSMTHVPVLYTQAMEGLRVVENGTYLDGTFGRGGHARGVLQQLGPGG
CCCHHHHHHHCCCCCHHHHHHHHHHHHHCCEEECCCCEECCCCCCCCHHHHHHHHCCCCC
RLLVMDKDPEAIAMAERAFSCDPRVVIRHGSFALLAQLAAPQSLDGVLFDLGVSSPQLDV
EEEEEECCCHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCEEEECCCCCCCCCC
PERGFSFAKDGPLDMRMDPEMGESAAQWLARVSEREIADVLWTYGEEKQSRRIARAIVAY
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RANQPLLRTVQLAELIASVMLRTKFGACKSRIHPATRSFQGIRIHVNRELVDLEVGLEAA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEECCEEEEHHHHHHHH
LAALRPGGRLVVISFHSLEDRIVKQFISRHSKVPPTNRRLPEVQTFVPLLRMIGRAIKAD
HHHHCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
EDELEVNPRARSAVLRVAEKLDVLGAVR
CCCEECCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCDEGCLDGLAPHLSMTHVPVLYTQAMEGLRVVENGTYLDGTFGRGGHARGVLQQLGPGG
CCCHHHHHHHCCCCCHHHHHHHHHHHHHCCEEECCCCEECCCCCCCCHHHHHHHHCCCCC
RLLVMDKDPEAIAMAERAFSCDPRVVIRHGSFALLAQLAAPQSLDGVLFDLGVSSPQLDV
EEEEEECCCHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCEEEECCCCCCCCCC
PERGFSFAKDGPLDMRMDPEMGESAAQWLARVSEREIADVLWTYGEEKQSRRIARAIVAY
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RANQPLLRTVQLAELIASVMLRTKFGACKSRIHPATRSFQGIRIHVNRELVDLEVGLEAA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEECCEEEEHHHHHHHH
LAALRPGGRLVVISFHSLEDRIVKQFISRHSKVPPTNRRLPEVQTFVPLLRMIGRAIKAD
HHHHCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
EDELEVNPRARSAVLRVAEKLDVLGAVR
CCCEECCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA