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The map label for this gene is pykA [H]

Identifier: 182682461

GI number: 182682461

Start: 2178993

End: 2180459

Strand: Reverse

Name: pykA [H]

Synonym: XfasM23_1948

Alternate gene names: 182682461

Gene position: 2180459-2178993 (Counterclockwise)

Preceding gene: 182682462

Following gene: 182682460

Centisome position: 85.99

GC content: 55.08

Gene sequence:

>1467_bases
ATGTCTGAACGCCAGCGCCGTACCAGGATTCTTGCCACTCTTGGGCCAGCGACAGATCCGCCTGGTGTGCTTGATGCGCT
GTTTAAGGCGGGAGTTAATGTGGTGCGGCTGAATTTCAGCCATGGCGATGTTTCGGATCAGGCCAGGCGTGTTGCTGAAG
TGCGTGCGGCGGCGGCGCATGTGGGCGTTGAGATTGGCATTCTTGCGGATCTACCCGGCCCGAAGATCCGTATTGGACGT
TTTACCGGGGGAAAAGTGCGGCTTGTGGCTGGTGCGCGCTTTGATCTGCTTGCCGATAGCAATGCGTCGCTGGGGGATAC
GACTCAAGTTGGGGTCAGTTATCTGGGGTTGCCGCAGGATGTCGCCGCCGGAGATGTGTTGTTACTGGATGATGGCCTCA
TGCAATTGCAAGTGGTGCAAGTGCAGGGTGCGCGGATTGTGACGACGGTTCTTAACGATGGTGTGTTGTCCGACCGTAAG
GGGCTGAATAAGCAAGGTGGCGGTTTGTCGCTGGGTGCGCTGACGGATCGTGATCGCGAACTGATTGGTATCGTTGCCAG
GATGGGGATCGACTTTATTGCAGTTTCGTTTTGTCGTCATGCTGAAGAGATGCATGAGGCGCGTCGCATTGCTCGGGAGT
GCGGCTGTGATGCTGCGTTGGTGTCTAAGATCGAGCGTGCTGAGGCTATCGTGAATCTTGCCGAGATTGTTGCGGCTTCG
GATGTGGTGATGGTTGCGCGTGGTGATTTAGGTGTGGAGATTGGTGACGCTCAATTGCCGGGCTTGCAAAAAAAGATCAT
CAAAGAAGCGTTGTTACAGAACAAGGTGGTGATTACTGCCACGCAGATGCTGCAATCAATGGTTGAAAGTCCGATACCGA
CCCGTGCTGAGGTGCTGGATGTGGCCAATGCGGTGATTGATGGGACCGATGCGGTGATGCTGTCGGCTGAAACGGCGACA
GGTGATTATCCAGTCAAGGCAGTAGAGGCGATGGCGCGTATCTGTCTGGGAGCGGAACACCAGTTTGAGTTTGATACTGA
TTATGAGATGGCGCAGCGTAATTTGCAGCGTGCAGATCATGCGATTGCAATGGCAACGATGTTCTTATCTGAGCACATTT
GCCTTGGTGGTGTGGTTGCGTTGACGGAGTCCGGTACGACGCCACGGTTTCTCTCGCGGTTCCGGTCGCACGTCCCGATC
TATGCGTTTACGCGTCACGAGGATGTGCGGCGGCGCATGGTGCTGATGCGTGGTGTATTTCCGATGCGTTTTGACAGCCG
TGGTTTAACGCCGCGTGAGGCAGCTAGGGCGACAATCCGTCTTTTGGTGGAATGTGGTTGTATGAGTCCGGGTGATCGTG
TGGTGTTCACGAGCGGCGAGCATATGGAAACGCTTGGCGCAACCAATACACTGCGTTTACTTGAAGTAGGCGCTGATGGG
TGTGTCAGTGGTCTGTGGGAACTCTAA

Upstream 100 bases:

>100_bases
TGCTGCGACATGAGAGGTATGTCCATCCTGAAAATAAGTAACATTCTGGAAAAAACAGCCTGTGTTATTTTTGCTAGCTT
CTGTGAAAGAGGCTTTCTTT

Downstream 100 bases:

>100_bases
GCTTGCGGCTTGCGGATACAAGTAGGTTTCACTGTAGTAGCGCCCACGTGTGGCCGTCCTTGGTGAAGAGAAGATTTCGC
CGTTGTGGCTTGATGTAGCA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSERQRRTRILATLGPATDPPGVLDALFKAGVNVVRLNFSHGDVSDQARRVAEVRAAAAHVGVEIGILADLPGPKIRIGR
FTGGKVRLVAGARFDLLADSNASLGDTTQVGVSYLGLPQDVAAGDVLLLDDGLMQLQVVQVQGARIVTTVLNDGVLSDRK
GLNKQGGGLSLGALTDRDRELIGIVARMGIDFIAVSFCRHAEEMHEARRIARECGCDAALVSKIERAEAIVNLAEIVAAS
DVVMVARGDLGVEIGDAQLPGLQKKIIKEALLQNKVVITATQMLQSMVESPIPTRAEVLDVANAVIDGTDAVMLSAETAT
GDYPVKAVEAMARICLGAEHQFEFDTDYEMAQRNLQRADHAIAMATMFLSEHICLGGVVALTESGTTPRFLSRFRSHVPI
YAFTRHEDVRRRMVLMRGVFPMRFDSRGLTPREAARATIRLLVECGCMSPGDRVVFTSGEHMETLGATNTLRLLEVGADG
CVSGLWEL

Sequences:

>Translated_488_residues
MSERQRRTRILATLGPATDPPGVLDALFKAGVNVVRLNFSHGDVSDQARRVAEVRAAAAHVGVEIGILADLPGPKIRIGR
FTGGKVRLVAGARFDLLADSNASLGDTTQVGVSYLGLPQDVAAGDVLLLDDGLMQLQVVQVQGARIVTTVLNDGVLSDRK
GLNKQGGGLSLGALTDRDRELIGIVARMGIDFIAVSFCRHAEEMHEARRIARECGCDAALVSKIERAEAIVNLAEIVAAS
DVVMVARGDLGVEIGDAQLPGLQKKIIKEALLQNKVVITATQMLQSMVESPIPTRAEVLDVANAVIDGTDAVMLSAETAT
GDYPVKAVEAMARICLGAEHQFEFDTDYEMAQRNLQRADHAIAMATMFLSEHICLGGVVALTESGTTPRFLSRFRSHVPI
YAFTRHEDVRRRMVLMRGVFPMRFDSRGLTPREAARATIRLLVECGCMSPGDRVVFTSGEHMETLGATNTLRLLEVGADG
CVSGLWEL
>Mature_487_residues
SERQRRTRILATLGPATDPPGVLDALFKAGVNVVRLNFSHGDVSDQARRVAEVRAAAAHVGVEIGILADLPGPKIRIGRF
TGGKVRLVAGARFDLLADSNASLGDTTQVGVSYLGLPQDVAAGDVLLLDDGLMQLQVVQVQGARIVTTVLNDGVLSDRKG
LNKQGGGLSLGALTDRDRELIGIVARMGIDFIAVSFCRHAEEMHEARRIARECGCDAALVSKIERAEAIVNLAEIVAASD
VVMVARGDLGVEIGDAQLPGLQKKIIKEALLQNKVVITATQMLQSMVESPIPTRAEVLDVANAVIDGTDAVMLSAETATG
DYPVKAVEAMARICLGAEHQFEFDTDYEMAQRNLQRADHAIAMATMFLSEHICLGGVVALTESGTTPRFLSRFRSHVPIY
AFTRHEDVRRRMVLMRGVFPMRFDSRGLTPREAARATIRLLVECGCMSPGDRVVFTSGEHMETLGATNTLRLLEVGADGC
VSGLWEL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=496, Percent_Identity=35.4838709677419, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI33286420, Length=496, Percent_Identity=35.4838709677419, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI33286418, Length=493, Percent_Identity=35.2941176470588, Blast_Score=250, Evalue=3e-66,
Organism=Homo sapiens, GI10835121, Length=493, Percent_Identity=34.6855983772819, Blast_Score=242, Evalue=5e-64,
Organism=Homo sapiens, GI32967597, Length=493, Percent_Identity=34.6855983772819, Blast_Score=241, Evalue=9e-64,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=34.7222222222222, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=34.7222222222222, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=32.6271186440678, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=32.6271186440678, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=32.093023255814, Blast_Score=106, Evalue=6e-23,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=50, Blast_Score=447, Evalue=1e-126,
Organism=Escherichia coli, GI1787965, Length=467, Percent_Identity=37.0449678800857, Blast_Score=294, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=35.8606557377049, Blast_Score=278, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI17506831, Length=490, Percent_Identity=37.3469387755102, Blast_Score=245, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI71984406, Length=490, Percent_Identity=37.3469387755102, Blast_Score=245, Evalue=5e-65,
Organism=Caenorhabditis elegans, GI71984413, Length=490, Percent_Identity=37.3469387755102, Blast_Score=244, Evalue=6e-65,
Organism=Caenorhabditis elegans, GI17506829, Length=490, Percent_Identity=37.3469387755102, Blast_Score=244, Evalue=7e-65,
Organism=Saccharomyces cerevisiae, GI6319279, Length=490, Percent_Identity=35.5102040816327, Blast_Score=259, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6324923, Length=491, Percent_Identity=34.826883910387, Blast_Score=248, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24648964, Length=490, Percent_Identity=35.5102040816327, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI28571814, Length=490, Percent_Identity=35.5102040816327, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24648966, Length=411, Percent_Identity=35.7664233576642, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24581235, Length=433, Percent_Identity=30.0230946882217, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24646914, Length=278, Percent_Identity=36.6906474820144, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52486; Mature: 52355

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERQRRTRILATLGPATDPPGVLDALFKAGVNVVRLNFSHGDVSDQARRVAEVRAAAAH
CCCHHHHHHHHEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
VGVEIGILADLPGPKIRIGRFTGGKVRLVAGARFDLLADSNASLGDTTQVGVSYLGLPQD
CCEEEEEEECCCCCCEEEEEECCCEEEEEECCEEEEEECCCCCCCCCHHHHHHHCCCCCH
VAAGDVLLLDDGLMQLQVVQVQGARIVTTVLNDGVLSDRKGLNKQGGGLSLGALTDRDRE
HCCCCEEEEECCCEEEEEEEECCCEEEEEHHCCCCCCCCCCCCCCCCCEEECCCCCCCHH
LIGIVARMGIDFIAVSFCRHAEEMHEARRIARECGCDAALVSKIERAEAIVNLAEIVAAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
DVVMVARGDLGVEIGDAQLPGLQKKIIKEALLQNKVVITATQMLQSMVESPIPTRAEVLD
CEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHCCCCCHHHHHH
VANAVIDGTDAVMLSAETATGDYPVKAVEAMARICLGAEHQFEFDTDYEMAQRNLQRADH
HHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AIAMATMFLSEHICLGGVVALTESGTTPRFLSRFRSHVPIYAFTRHEDVRRRMVLMRGVF
HHHHHHHHHHHCHHHCCEEEEECCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCC
PMRFDSRGLTPREAARATIRLLVECGCMSPGDRVVFTSGEHMETLGATNTLRLLEVGADG
CCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCCCEEEEEEECCCH
CVSGLWEL
HHHHHHCC
>Mature Secondary Structure 
SERQRRTRILATLGPATDPPGVLDALFKAGVNVVRLNFSHGDVSDQARRVAEVRAAAAH
CCHHHHHHHHEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
VGVEIGILADLPGPKIRIGRFTGGKVRLVAGARFDLLADSNASLGDTTQVGVSYLGLPQD
CCEEEEEEECCCCCCEEEEEECCCEEEEEECCEEEEEECCCCCCCCCHHHHHHHCCCCCH
VAAGDVLLLDDGLMQLQVVQVQGARIVTTVLNDGVLSDRKGLNKQGGGLSLGALTDRDRE
HCCCCEEEEECCCEEEEEEEECCCEEEEEHHCCCCCCCCCCCCCCCCCEEECCCCCCCHH
LIGIVARMGIDFIAVSFCRHAEEMHEARRIARECGCDAALVSKIERAEAIVNLAEIVAAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
DVVMVARGDLGVEIGDAQLPGLQKKIIKEALLQNKVVITATQMLQSMVESPIPTRAEVLD
CEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHCCCCCHHHHHH
VANAVIDGTDAVMLSAETATGDYPVKAVEAMARICLGAEHQFEFDTDYEMAQRNLQRADH
HHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
AIAMATMFLSEHICLGGVVALTESGTTPRFLSRFRSHVPIYAFTRHEDVRRRMVLMRGVF
HHHHHHHHHHHCHHHCCEEEEECCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCC
PMRFDSRGLTPREAARATIRLLVECGCMSPGDRVVFTSGEHMETLGATNTLRLLEVGADG
CCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCCCEEEEEEECCCH
CVSGLWEL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]