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The map label for this gene is metB [H]

Identifier: 182682428

GI number: 182682428

Start: 2132366

End: 2133568

Strand: Reverse

Name: metB [H]

Synonym: XfasM23_1914

Alternate gene names: 182682428

Gene position: 2133568-2132366 (Counterclockwise)

Preceding gene: 182682429

Following gene: 182682427

Centisome position: 84.14

GC content: 55.94

Gene sequence:

>1203_bases
ATGAGCATTCATGATGTGCACGAAGGCACGTGTCACGTGACCACTTCCGCGGTGCGTGCTGGGATCGATTCCGACACTGC
TCATGGGGCAGTGACGCCACCGATTGTGCTTTCATCCAACTTCTCGTTTGAACGTTTCGGTCATAAGCGTAAGTACGATT
ACACACGTAGTGCTAATCCAACGCGGGATCTGCTCGGCGAGGCGCTTGCGGAGTTGGAGGGGGGTGCCGGTGGTGTGGTT
ACGGCGACTGGGATCGCTGCGATTACGTTGGTGTTGCACGCATTGCTCCGGCCGGGTGATCGTTTGGTTGTGCCACATGA
TGCTTACGGTGGCAGTTGGCGTTTGTTCAATGCGTTGGCTCAAAAGGGACATTTTGAATTGATCACTGTCGATTTGACGG
ATGCACGATCACTGGTTGAGGCATTGGCACTACCACCGAAGTTGGTATTGATCGAAACTCCTTCCAATCCATTGTTACGT
ATTACTGATTTGGGTGCGGTGATTGAGGCAGCGCACCGTGTCGGTGCTTTGACTGTGGTCGACAATACGTTTATGTCTCC
GGTGTTGCAGCGTCCTTTAGAGTTCGGCGCTGACTTGGTATTGCATTCGACCACGAAGTACATCAATGGCCACAGTGATG
TTGTCGGTGGCGTTGTTGTGGCACGTGATGTTGCGCACTATCAGCAGTTGGTGTGGTGGGCGAATGCGCTGGGTCTGACC
GGCTCGCCATTCGATGCCTTCTTGACGTTGCGGGGGGTGCGCACGCTGGATGCGCGTTTGCGTGTGCATCAGGAGAATGC
GCAGATGATTGCAGCCTTACTGGAGGGGCATGGTGTGGTGAAGCACGTGCATTACCCAGGATTAGCTTCGCATCCGGGGC
ATGTGCTGGCGGGGCGGCAGCAAAAAGGGTTCGGTGCCATGCTCAGTTTCGAGTTGCAGGGTGGGGAAGAGGCGGTGCGG
GCTTTTGTCAGTGGCTTGCGTTATTTCACGCTGGCTGAGTCGCTGGGTGGGGTTGAGAGTTTGATCGCTCATCCGGCTTC
GATGACGCATGCTGCCATGACGCCCGAGGCGCGTGCTAAAGCGGGTATCTCTGATGGGTTGTTGCGCTTGTCGGTGGGGA
TTGAGGCCAGTGAGGATTTGATCGTTGATCTGGTGGCTGGTTTGGGGCGTGCTCAGGCGGTGCGGGAAGAGTGCAAGGTG
TGA

Upstream 100 bases:

>100_bases
GGGTGTTGCGTTCTCCTTACGGTCACGATGCTTTTTTAAAAGAAACTAATCGCATTGATGCGATTCTTACGACAGCGCTA
CGTCCTATAGGAGAATCCAT

Downstream 100 bases:

>100_bases
GTATTGGGGCGGGTCATTTGTTTACTCGTTAGTCCGATGTTCGTTGTGCTGGATGATGCACGTTGATGTGTGTGTTAACG
GTTGCTTCGGTGACTCGTTG

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSIHDVHEGTCHVTTSAVRAGIDSDTAHGAVTPPIVLSSNFSFERFGHKRKYDYTRSANPTRDLLGEALAELEGGAGGVV
TATGIAAITLVLHALLRPGDRLVVPHDAYGGSWRLFNALAQKGHFELITVDLTDARSLVEALALPPKLVLIETPSNPLLR
ITDLGAVIEAAHRVGALTVVDNTFMSPVLQRPLEFGADLVLHSTTKYINGHSDVVGGVVVARDVAHYQQLVWWANALGLT
GSPFDAFLTLRGVRTLDARLRVHQENAQMIAALLEGHGVVKHVHYPGLASHPGHVLAGRQQKGFGAMLSFELQGGEEAVR
AFVSGLRYFTLAESLGGVESLIAHPASMTHAAMTPEARAKAGISDGLLRLSVGIEASEDLIVDLVAGLGRAQAVREECKV

Sequences:

>Translated_400_residues
MSIHDVHEGTCHVTTSAVRAGIDSDTAHGAVTPPIVLSSNFSFERFGHKRKYDYTRSANPTRDLLGEALAELEGGAGGVV
TATGIAAITLVLHALLRPGDRLVVPHDAYGGSWRLFNALAQKGHFELITVDLTDARSLVEALALPPKLVLIETPSNPLLR
ITDLGAVIEAAHRVGALTVVDNTFMSPVLQRPLEFGADLVLHSTTKYINGHSDVVGGVVVARDVAHYQQLVWWANALGLT
GSPFDAFLTLRGVRTLDARLRVHQENAQMIAALLEGHGVVKHVHYPGLASHPGHVLAGRQQKGFGAMLSFELQGGEEAVR
AFVSGLRYFTLAESLGGVESLIAHPASMTHAAMTPEARAKAGISDGLLRLSVGIEASEDLIVDLVAGLGRAQAVREECKV
>Mature_399_residues
SIHDVHEGTCHVTTSAVRAGIDSDTAHGAVTPPIVLSSNFSFERFGHKRKYDYTRSANPTRDLLGEALAELEGGAGGVVT
ATGIAAITLVLHALLRPGDRLVVPHDAYGGSWRLFNALAQKGHFELITVDLTDARSLVEALALPPKLVLIETPSNPLLRI
TDLGAVIEAAHRVGALTVVDNTFMSPVLQRPLEFGADLVLHSTTKYINGHSDVVGGVVVARDVAHYQQLVWWANALGLTG
SPFDAFLTLRGVRTLDARLRVHQENAQMIAALLEGHGVVKHVHYPGLASHPGHVLAGRQQKGFGAMLSFELQGGEEAVRA
FVSGLRYFTLAESLGGVESLIAHPASMTHAAMTPEARAKAGISDGLLRLSVGIEASEDLIVDLVAGLGRAQAVREECKV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=364, Percent_Identity=42.032967032967, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI299473759, Length=364, Percent_Identity=38.7362637362637, Blast_Score=238, Evalue=9e-63,
Organism=Homo sapiens, GI25453487, Length=363, Percent_Identity=36.6391184573003, Blast_Score=220, Evalue=2e-57,
Organism=Escherichia coli, GI1790375, Length=374, Percent_Identity=58.0213903743316, Blast_Score=443, Evalue=1e-126,
Organism=Escherichia coli, GI1789383, Length=389, Percent_Identity=31.1053984575836, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17560056, Length=368, Percent_Identity=44.8369565217391, Blast_Score=313, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17538055, Length=368, Percent_Identity=45.1086956521739, Blast_Score=312, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=37.027027027027, Blast_Score=226, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6319307, Length=387, Percent_Identity=42.6356589147287, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6321254, Length=411, Percent_Identity=33.8199513381995, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6323333, Length=429, Percent_Identity=29.8368298368298, Blast_Score=149, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6321904, Length=397, Percent_Identity=30.4785894206549, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321170, Length=330, Percent_Identity=27.8787878787879, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6323557, Length=252, Percent_Identity=26.984126984127, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322970, Length=246, Percent_Identity=28.4552845528455, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922554, Length=377, Percent_Identity=41.1140583554377, Blast_Score=285, Evalue=3e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 42561; Mature: 42430

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIHDVHEGTCHVTTSAVRAGIDSDTAHGAVTPPIVLSSNFSFERFGHKRKYDYTRSANP
CCCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCCCCCCCCCC
TRDLLGEALAELEGGAGGVVTATGIAAITLVLHALLRPGDRLVVPHDAYGGSWRLFNALA
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHH
QKGHFELITVDLTDARSLVEALALPPKLVLIETPSNPLLRITDLGAVIEAAHRVGALTVV
CCCCEEEEEEECHHHHHHHHHHCCCCEEEEEECCCCCEEEEEHHHHHHHHHHHCCEEEEE
DNTFMSPVLQRPLEFGADLVLHSTTKYINGHSDVVGGVVVARDVAHYQQLVWWANALGLT
CHHHHHHHHHHHHHHCCCEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GSPFDAFLTLRGVRTLDARLRVHQENAQMIAALLEGHGVVKHVHYPGLASHPGHVLAGRQ
CCCHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEECCC
QKGFGAMLSFELQGGEEAVRAFVSGLRYFTLAESLGGVESLIAHPASMTHAAMTPEARAK
CCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHCCCHHHHH
AGISDGLLRLSVGIEASEDLIVDLVAGLGRAQAVREECKV
CCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
SIHDVHEGTCHVTTSAVRAGIDSDTAHGAVTPPIVLSSNFSFERFGHKRKYDYTRSANP
CCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCCCCCCCCCC
TRDLLGEALAELEGGAGGVVTATGIAAITLVLHALLRPGDRLVVPHDAYGGSWRLFNALA
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHH
QKGHFELITVDLTDARSLVEALALPPKLVLIETPSNPLLRITDLGAVIEAAHRVGALTVV
CCCCEEEEEEECHHHHHHHHHHCCCCEEEEEECCCCCEEEEEHHHHHHHHHHHCCEEEEE
DNTFMSPVLQRPLEFGADLVLHSTTKYINGHSDVVGGVVVARDVAHYQQLVWWANALGLT
CHHHHHHHHHHHHHHCCCEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GSPFDAFLTLRGVRTLDARLRVHQENAQMIAALLEGHGVVKHVHYPGLASHPGHVLAGRQ
CCCHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEECCC
QKGFGAMLSFELQGGEEAVRAFVSGLRYFTLAESLGGVESLIAHPASMTHAAMTPEARAK
CCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHCCCHHHHH
AGISDGLLRLSVGIEASEDLIVDLVAGLGRAQAVREECKV
CCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]