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The map label for this gene is rlmH [H]

Identifier: 182682324

GI number: 182682324

Start: 2018737

End: 2019210

Strand: Direct

Name: rlmH [H]

Synonym: XfasM23_1807

Alternate gene names: 182682324

Gene position: 2018737-2019210 (Clockwise)

Preceding gene: 182682322

Following gene: 182682326

Centisome position: 79.61

GC content: 48.95

Gene sequence:

>474_bases
ATGAAATGTCTCCTCATTGCAACTGGTCAACATGTTCCAACCTGGGTTGCGCAAGGCTTTGCTGAGTACCACAGACGTCT
TTCTTACTGGTTACCGCTTGAGTTGGTTGAAATCGAACCCAGCATGCGCGGCAAAAACCACGATCCGCAACGAGCCATAG
AAGACGAAGGACGGCGCGTCATGGCCGCTTTACCTAAACAACCTTATGCCGTCACCTTGGACGTGAAAGGTAAGCCCCTC
AATTCAGAACAATTAGCACAGCGCATGGAACACTGGCGTGGATTAGGACGTAATTTAGTATTCTTGATCGGCGGACCTGA
AGGGCATAGTCAGGAAGTACTTAATATCTCCAACGAGCGCTGGTCATTAGGGCCACTCACGCTACCACACATGCTGGTAA
GGCTGATCGTCGTTGAACAATTATATCGAGCAGCAACGATACTCACTAACCACCCATACCACAGAGGAAAATAA

Upstream 100 bases:

>100_bases
TGTGTTGTTTTCATTCAGACTGAGACATGCAAATGCAACAATCCCGGTATCGGCAACCTAAGTATTAAGATAGAGCGTGA
ATTAACCCTTAGTCTCAGTG

Downstream 100 bases:

>100_bases
GAAACTCTAAATTAAAAAGATTACATTTTAGAAAAAGATAGACATTTATTATGCCGCTGATTAATAAAATCCCACACTGC
AACATTTCACCCTATTAAAT

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MKCLLIATGQHVPTWVAQGFAEYHRRLSYWLPLELVEIEPSMRGKNHDPQRAIEDEGRRVMAALPKQPYAVTLDVKGKPL
NSEQLAQRMEHWRGLGRNLVFLIGGPEGHSQEVLNISNERWSLGPLTLPHMLVRLIVVEQLYRAATILTNHPYHRGK

Sequences:

>Translated_157_residues
MKCLLIATGQHVPTWVAQGFAEYHRRLSYWLPLELVEIEPSMRGKNHDPQRAIEDEGRRVMAALPKQPYAVTLDVKGKPL
NSEQLAQRMEHWRGLGRNLVFLIGGPEGHSQEVLNISNERWSLGPLTLPHMLVRLIVVEQLYRAATILTNHPYHRGK
>Mature_157_residues
MKCLLIATGQHVPTWVAQGFAEYHRRLSYWLPLELVEIEPSMRGKNHDPQRAIEDEGRRVMAALPKQPYAVTLDVKGKPL
NSEQLAQRMEHWRGLGRNLVFLIGGPEGHSQEVLNISNERWSLGPLTLPHMLVRLIVVEQLYRAATILTNHPYHRGK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA [H]

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family [H]

Homologues:

Organism=Escherichia coli, GI1786855, Length=155, Percent_Identity=49.6774193548387, Blast_Score=151, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016051
- InterPro:   IPR003742 [H]

Pfam domain/function: PF02590 SPOUT_MTase [H]

EC number: NA

Molecular weight: Translated: 17985; Mature: 17985

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCLLIATGQHVPTWVAQGFAEYHRRLSYWLPLELVEIEPSMRGKNHDPQRAIEDEGRRV
CEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHCCCEE
MAALPKQPYAVTLDVKGKPLNSEQLAQRMEHWRGLGRNLVFLIGGPEGHSQEVLNISNER
EECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHEECCCCC
WSLGPLTLPHMLVRLIVVEQLYRAATILTNHPYHRGK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKCLLIATGQHVPTWVAQGFAEYHRRLSYWLPLELVEIEPSMRGKNHDPQRAIEDEGRRV
CEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHCCCEE
MAALPKQPYAVTLDVKGKPLNSEQLAQRMEHWRGLGRNLVFLIGGPEGHSQEVLNISNER
EECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHEECCCCC
WSLGPLTLPHMLVRLIVVEQLYRAATILTNHPYHRGK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]