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The map label for this gene is eptA [H]

Identifier: 182682300

GI number: 182682300

Start: 1978576

End: 1980234

Strand: Reverse

Name: eptA [H]

Synonym: XfasM23_1783

Alternate gene names: 182682300

Gene position: 1980234-1978576 (Counterclockwise)

Preceding gene: 182682301

Following gene: 182682297

Centisome position: 78.09

GC content: 49.25

Gene sequence:

>1659_bases
ATGAGTTTTTCAACATTTGTTCTTGTGTTACGGCGTCGGATGAGTGAGTTTAACTGGCGTGTGCGCCCGGAAGTTTCTAC
TGAGAGTGTAGTGCTCTGTACTAGCCTGTTCTTTGCACTTGCTTGCAATACGATGTTCTGGCGAAGTGCGATGAGTACCG
TCTCAGGAAGTATCGGTTTTGTCCTTTCGCTTTTAGCGTTGTTGGTGACGGTACATGCGTTGCTGTTGGGTTTGGTGGTG
TGGCGGTGGAGTGCAAAGCCATTGTTGACTTTGTTATTCGTGATTACGGCGTTTGCTACGCATTACATGAATAGCTACAG
CGTCTACCTGGACGCGGACATGCTGCGTAATGTGTTCAATACCGATCATAAAGAGTCTCGGGAGCTGATAACTTCAGCGC
TAATTTTGCCGTTGCTGTTCTATGCGGCGGTACCAATTGCGGTGTTGTGGCGGCTACGGTTTCGCCAGCGACCTTGGTCT
CGTGCGCTTGGCCTACGCATGTTGTTTTTGCTGATAGTTATTGTGGTTGGTGCCAGTGGTGCGATGCTGTCATTCCAAAA
ATTGTCGGCGTTGATACGTAATGATCGTGAGGTGCGTCATTTAGCCACGCCCATCAATTACATCATGGCGTTGCGCAAGA
TGTTGAGCAATGATTCGGTTTTGAAGCGCGCTCCCAAGTTGCCGATTGGTGAAGATGCGGTGGCTACCCCACGTGTACCA
AGTAGTCGTCCGCGTTTGTTAGTAATCGTAGTTGGTGAAACTGCCAGGGCACAGAATTGGGGGCTGAATGGCTACGTCCG
TCAGACAACTCCGCAATTGGCTCAGAACGACGTTATCAATTTCCAAGATATGCACTCGTGTGGAACTAATACTGAAGTTT
CGGTGCCATGCATGTTTTCACCGTATGGTCGTCGCAATTATGACGAACGTAAAATTCGTGGGCATCAGTCGCTATTACAT
GTGCTTGAGCGTGCCCGAATCAGTACGTTGTGGCGCGATAACCAGTCTGGCTGTAAGGGGGTATGTGATGGATTGGAGTT
GCAGCAGTTAGATGATGCTAAAGATCCCACGCTGTGCACTAGTAGTGGTCGCTGTATGGATGAAATTCTACTGAAGGATT
TCGTGTCGCAGGTACGCAGTAAGTCGGGGGATCGGGTGGTGGTGCTTCATCAGCTTGGTAGCCACGGTCCCAGTTATTTC
CAGCGTTATCCAGTTGCGTTCCGTCAATTTAATCCGACGTGTGAGACTCCTAACCTGGGGAGTTGCAGCCGTGAGCAGAT
CGTTGCTGCTTACGATAATAGTTTGCTTTATACCGACCACTTTCTTGTCCGGACGATTGGGATGCTGCGTGATATGTCCG
ACTACGACACAGCGATGATTTATTTATCCGATCACGGTGAATCTCTTGGTGAAAAGGGACTTTATTTGCATGGTATGCCA
TACGCAATTGCGCCTGTTGAGCAGACACGGGTGCCGATGGTGATATGGTTCTCGAAGCAGTTCGTTCAGTCACGTCAGAT
AGACTTGAACTGTGTGCACCAACGTGCCCGTCAGTATGCTGACCATGACAATCTATTCTCATCGGTGTTGGGGTTGATGC
AGGTCAAGACAGCGCTATATGAGCGTCCACATGATCTGTTTGCCACATGCGAGAAATGA

Upstream 100 bases:

>100_bases
GGTCACTGACCATCTGTTGGTTGGTTGCTTTGGGCTTTTTCTACCTCTTTTTTGTTTCTCCCGCAGTAAGGGTAGTCTCT
GCGCAACAAAGGGCAATGAC

Downstream 100 bases:

>100_bases
TGGGGATATTATGGTTGTTCATGTCTCATCGGACTAAATCTGAAGGAGTCATCACATCGTTTTACGGTGTCTGGTTTATG
GACTAAAAATTTTATGATCT

Product: sulfatase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MSFSTFVLVLRRRMSEFNWRVRPEVSTESVVLCTSLFFALACNTMFWRSAMSTVSGSIGFVLSLLALLVTVHALLLGLVV
WRWSAKPLLTLLFVITAFATHYMNSYSVYLDADMLRNVFNTDHKESRELITSALILPLLFYAAVPIAVLWRLRFRQRPWS
RALGLRMLFLLIVIVVGASGAMLSFQKLSALIRNDREVRHLATPINYIMALRKMLSNDSVLKRAPKLPIGEDAVATPRVP
SSRPRLLVIVVGETARAQNWGLNGYVRQTTPQLAQNDVINFQDMHSCGTNTEVSVPCMFSPYGRRNYDERKIRGHQSLLH
VLERARISTLWRDNQSGCKGVCDGLELQQLDDAKDPTLCTSSGRCMDEILLKDFVSQVRSKSGDRVVVLHQLGSHGPSYF
QRYPVAFRQFNPTCETPNLGSCSREQIVAAYDNSLLYTDHFLVRTIGMLRDMSDYDTAMIYLSDHGESLGEKGLYLHGMP
YAIAPVEQTRVPMVIWFSKQFVQSRQIDLNCVHQRARQYADHDNLFSSVLGLMQVKTALYERPHDLFATCEK

Sequences:

>Translated_552_residues
MSFSTFVLVLRRRMSEFNWRVRPEVSTESVVLCTSLFFALACNTMFWRSAMSTVSGSIGFVLSLLALLVTVHALLLGLVV
WRWSAKPLLTLLFVITAFATHYMNSYSVYLDADMLRNVFNTDHKESRELITSALILPLLFYAAVPIAVLWRLRFRQRPWS
RALGLRMLFLLIVIVVGASGAMLSFQKLSALIRNDREVRHLATPINYIMALRKMLSNDSVLKRAPKLPIGEDAVATPRVP
SSRPRLLVIVVGETARAQNWGLNGYVRQTTPQLAQNDVINFQDMHSCGTNTEVSVPCMFSPYGRRNYDERKIRGHQSLLH
VLERARISTLWRDNQSGCKGVCDGLELQQLDDAKDPTLCTSSGRCMDEILLKDFVSQVRSKSGDRVVVLHQLGSHGPSYF
QRYPVAFRQFNPTCETPNLGSCSREQIVAAYDNSLLYTDHFLVRTIGMLRDMSDYDTAMIYLSDHGESLGEKGLYLHGMP
YAIAPVEQTRVPMVIWFSKQFVQSRQIDLNCVHQRARQYADHDNLFSSVLGLMQVKTALYERPHDLFATCEK
>Mature_551_residues
SFSTFVLVLRRRMSEFNWRVRPEVSTESVVLCTSLFFALACNTMFWRSAMSTVSGSIGFVLSLLALLVTVHALLLGLVVW
RWSAKPLLTLLFVITAFATHYMNSYSVYLDADMLRNVFNTDHKESRELITSALILPLLFYAAVPIAVLWRLRFRQRPWSR
ALGLRMLFLLIVIVVGASGAMLSFQKLSALIRNDREVRHLATPINYIMALRKMLSNDSVLKRAPKLPIGEDAVATPRVPS
SRPRLLVIVVGETARAQNWGLNGYVRQTTPQLAQNDVINFQDMHSCGTNTEVSVPCMFSPYGRRNYDERKIRGHQSLLHV
LERARISTLWRDNQSGCKGVCDGLELQQLDDAKDPTLCTSSGRCMDEILLKDFVSQVRSKSGDRVVVLHQLGSHGPSYFQ
RYPVAFRQFNPTCETPNLGSCSREQIVAAYDNSLLYTDHFLVRTIGMLRDMSDYDTAMIYLSDHGESLGEKGLYLHGMPY
AIAPVEQTRVPMVIWFSKQFVQSRQIDLNCVHQRARQYADHDNLFSSVLGLMQVKTALYERPHDLFATCEK

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=541, Percent_Identity=36.5988909426987, Blast_Score=370, Evalue=1e-103,
Organism=Escherichia coli, GI87082286, Length=520, Percent_Identity=28.8461538461538, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1790392, Length=491, Percent_Identity=21.9959266802444, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082223, Length=256, Percent_Identity=28.515625, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 62680; Mature: 62548

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS01319 RBFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSTFVLVLRRRMSEFNWRVRPEVSTESVVLCTSLFFALACNTMFWRSAMSTVSGSIGF
CCHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
VLSLLALLVTVHALLLGLVVWRWSAKPLLTLLFVITAFATHYMNSYSVYLDADMLRNVFN
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHC
TDHKESRELITSALILPLLFYAAVPIAVLWRLRFRQRPWSRALGLRMLFLLIVIVVGASG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
AMLSFQKLSALIRNDREVRHLATPINYIMALRKMLSNDSVLKRAPKLPIGEDAVATPRVP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCC
SSRPRLLVIVVGETARAQNWGLNGYVRQTTPQLAQNDVINFQDMHSCGTNTEVSVPCMFS
CCCCCEEEEEECCCCCCCCCCCCCEEECCCHHHHHCCCCCHHHHHCCCCCCCEEECEEEC
PYGRRNYDERKIRGHQSLLHVLERARISTLWRDNQSGCKGVCDGLELQQLDDAKDPTLCT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHCCCCCCCEEC
SSGRCMDEILLKDFVSQVRSKSGDRVVVLHQLGSHGPSYFQRYPVAFRQFNPTCETPNLG
CCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCHHHHHCCCCCCCCCCC
SCSREQIVAAYDNSLLYTDHFLVRTIGMLRDMSDYDTAMIYLSDHGESLGEKGLYLHGMP
CCCHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCC
YAIAPVEQTRVPMVIWFSKQFVQSRQIDLNCVHQRARQYADHDNLFSSVLGLMQVKTALY
CEECCHHHCCCCEEEEECHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
ERPHDLFATCEK
HCCCHHHHCCCC
>Mature Secondary Structure 
SFSTFVLVLRRRMSEFNWRVRPEVSTESVVLCTSLFFALACNTMFWRSAMSTVSGSIGF
CHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
VLSLLALLVTVHALLLGLVVWRWSAKPLLTLLFVITAFATHYMNSYSVYLDADMLRNVFN
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHC
TDHKESRELITSALILPLLFYAAVPIAVLWRLRFRQRPWSRALGLRMLFLLIVIVVGASG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
AMLSFQKLSALIRNDREVRHLATPINYIMALRKMLSNDSVLKRAPKLPIGEDAVATPRVP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCC
SSRPRLLVIVVGETARAQNWGLNGYVRQTTPQLAQNDVINFQDMHSCGTNTEVSVPCMFS
CCCCCEEEEEECCCCCCCCCCCCCEEECCCHHHHHCCCCCHHHHHCCCCCCCEEECEEEC
PYGRRNYDERKIRGHQSLLHVLERARISTLWRDNQSGCKGVCDGLELQQLDDAKDPTLCT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHCCCCCCCEEC
SSGRCMDEILLKDFVSQVRSKSGDRVVVLHQLGSHGPSYFQRYPVAFRQFNPTCETPNLG
CCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCHHHHHCCCCCCCCCCC
SCSREQIVAAYDNSLLYTDHFLVRTIGMLRDMSDYDTAMIYLSDHGESLGEKGLYLHGMP
CCCHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCC
YAIAPVEQTRVPMVIWFSKQFVQSRQIDLNCVHQRARQYADHDNLFSSVLGLMQVKTALY
CEECCHHHCCCCEEEEECHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
ERPHDLFATCEK
HCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8282725 [H]