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The map label for this gene is rlmI [H]

Identifier: 182682279

GI number: 182682279

Start: 1949765

End: 1950934

Strand: Reverse

Name: rlmI [H]

Synonym: XfasM23_1761

Alternate gene names: 182682279

Gene position: 1950934-1949765 (Counterclockwise)

Preceding gene: 182682281

Following gene: 182682276

Centisome position: 76.94

GC content: 53.76

Gene sequence:

>1170_bases
ATGTCTAGAGATCTCCCTGTTATCCGTCTTAAGAATGCTTGGCGTTCTAGCCACCCATGGCTGTTCCAAAAGCTCCTGGA
AAAACCTTCCGTCCGTCTGAAACCAGGCACTATTGTCGACGTCGTCGGCGTCGATGGAGAATGGGTTGGCCGTGGTTTCT
ACAATGGCCATTCGCGTATTGCGGTGCGCATTTTGGAAACCCGCCAAGATGTGGTGGTGGATGAAGCGTGGTTCGTGCGT
AGGATTGCCGAAGCTGTTGGTTTACGCCGCGAGGTACTGAAGTTGGATGCAGTGTCTGATGCTTGGCGCGTGGTACACAG
CGAGGGTGACGGCCTGTCCGGTTTAGTGGTGGACCGTTACGGTGACTTACTGGTCGTGCAATTCTTCGCTGCAGGTATGT
TCCGTCATCGAGAGTGGATCTACACCGCGTTACGCCAGCAGTTCCCAGGCGCTCGCTTCCACAGCTTCGCTGACGAACAT
GTTCAGAAGCAGGAGAGCTTCGACTTTCACAGCACGATTGATACTGAATCGGCGGTGATCACCGAGCATGGTGTCAAGTT
TCTTGTTGACCCGATGGGTGCACATAAGACCGGCTTCTTTGCCGATCAGCGCGAGAACCGCCAGTGGTTGAGCCAAGGTG
TTGCAGGCAAGACCGTCTTGGACCTGTGCTGCAATACCGGAGGATTTGCGGTCTATGCTGCTTTACGCGGTGCGGCGGAT
GTAGTGGGTGTGGACATTGACCAAGATGTCATTCAGATCGCCAAAGCCAATGCAAAGCTTAACAGCGTCCGCCCCAAATT
CTTGCAGACCGACATCTTTCCGTATCTGCGTGATGCCGCCGTACGTGGTGACCAGTACGACGTGGTGATCCTCGACCCTG
CCAAAATGACCCGCGACCGTGATCAGATCATCAACGCCCTGAAGAAATATCTAGACATGAATAAGCTGGCCTTGGGCGTA
GTCAAAAAGGGAGGCTTGTTTGCCACCTTTTCTTGTACTGGCTTGATTAGCGAAGAGCAGTTCCTAGAGATGCTGCGTCG
CGCGGCATTCTATTCCGGCCGTACCATCCAAATTCTCAAAGTGGCTGGCGCTGGTCCTGATCATCCGTTCATGGCGCATG
TACAAGAATCGCGTTACCTCAAAGCGACGTTTTGCCGCGTCCTGGATTGA

Upstream 100 bases:

>100_bases
TTGACGGTGACTAACGTGTAGGATCTATCCCCCTTTATAAAACCAATTTAGCGGGCAGAGTGTAAGCACGTCAACCAAGC
CGAACAACTACAATACCCCA

Downstream 100 bases:

>100_bases
GGGGTGAATTGCAAGACATTGCTTTAAAAAGCGTGGTTCTACGCAACTATTGAGTGCTTCAGTTGCTGCGTGGGCGACGT
AGCTTGATCCTGTAGGCGAT

Product: oxidoreductase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSRDLPVIRLKNAWRSSHPWLFQKLLEKPSVRLKPGTIVDVVGVDGEWVGRGFYNGHSRIAVRILETRQDVVVDEAWFVR
RIAEAVGLRREVLKLDAVSDAWRVVHSEGDGLSGLVVDRYGDLLVVQFFAAGMFRHREWIYTALRQQFPGARFHSFADEH
VQKQESFDFHSTIDTESAVITEHGVKFLVDPMGAHKTGFFADQRENRQWLSQGVAGKTVLDLCCNTGGFAVYAALRGAAD
VVGVDIDQDVIQIAKANAKLNSVRPKFLQTDIFPYLRDAAVRGDQYDVVILDPAKMTRDRDQIINALKKYLDMNKLALGV
VKKGGLFATFSCTGLISEEQFLEMLRRAAFYSGRTIQILKVAGAGPDHPFMAHVQESRYLKATFCRVLD

Sequences:

>Translated_389_residues
MSRDLPVIRLKNAWRSSHPWLFQKLLEKPSVRLKPGTIVDVVGVDGEWVGRGFYNGHSRIAVRILETRQDVVVDEAWFVR
RIAEAVGLRREVLKLDAVSDAWRVVHSEGDGLSGLVVDRYGDLLVVQFFAAGMFRHREWIYTALRQQFPGARFHSFADEH
VQKQESFDFHSTIDTESAVITEHGVKFLVDPMGAHKTGFFADQRENRQWLSQGVAGKTVLDLCCNTGGFAVYAALRGAAD
VVGVDIDQDVIQIAKANAKLNSVRPKFLQTDIFPYLRDAAVRGDQYDVVILDPAKMTRDRDQIINALKKYLDMNKLALGV
VKKGGLFATFSCTGLISEEQFLEMLRRAAFYSGRTIQILKVAGAGPDHPFMAHVQESRYLKATFCRVLD
>Mature_388_residues
SRDLPVIRLKNAWRSSHPWLFQKLLEKPSVRLKPGTIVDVVGVDGEWVGRGFYNGHSRIAVRILETRQDVVVDEAWFVRR
IAEAVGLRREVLKLDAVSDAWRVVHSEGDGLSGLVVDRYGDLLVVQFFAAGMFRHREWIYTALRQQFPGARFHSFADEHV
QKQESFDFHSTIDTESAVITEHGVKFLVDPMGAHKTGFFADQRENRQWLSQGVAGKTVLDLCCNTGGFAVYAALRGAADV
VGVDIDQDVIQIAKANAKLNSVRPKFLQTDIFPYLRDAAVRGDQYDVVILDPAKMTRDRDQIINALKKYLDMNKLALGVV
KKGGLFATFSCTGLISEEQFLEMLRRAAFYSGRTIQILKVAGAGPDHPFMAHVQESRYLKATFCRVLD

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=379, Percent_Identity=34.3007915567282, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1787181, Length=197, Percent_Identity=26.3959390862944, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43772; Mature: 43641

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRDLPVIRLKNAWRSSHPWLFQKLLEKPSVRLKPGTIVDVVGVDGEWVGRGFYNGHSRI
CCCCCCEEEECCCCCCCCCHHHHHHHHCCCCEECCCCEEEEEECCCCEECCCEECCCCEE
AVRILETRQDVVVDEAWFVRRIAEAVGLRREVLKLDAVSDAWRVVHSEGDGLSGLVVDRY
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCC
GDLLVVQFFAAGMFRHREWIYTALRQQFPGARFHSFADEHVQKQESFDFHSTIDTESAVI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
TEHGVKFLVDPMGAHKTGFFADQRENRQWLSQGVAGKTVLDLCCNTGGFAVYAALRGAAD
HHCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHCCCC
VVGVDIDQDVIQIAKANAKLNSVRPKFLQTDIFPYLRDAAVRGDQYDVVILDPAKMTRDR
EEECCCCHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCH
DQIINALKKYLDMNKLALGVVKKGGLFATFSCTGLISEEQFLEMLRRAAFYSGRTIQILK
HHHHHHHHHHHCHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEE
VAGAGPDHPFMAHVQESRYLKATFCRVLD
EECCCCCCCHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
SRDLPVIRLKNAWRSSHPWLFQKLLEKPSVRLKPGTIVDVVGVDGEWVGRGFYNGHSRI
CCCCCEEEECCCCCCCCCHHHHHHHHCCCCEECCCCEEEEEECCCCEECCCEECCCCEE
AVRILETRQDVVVDEAWFVRRIAEAVGLRREVLKLDAVSDAWRVVHSEGDGLSGLVVDRY
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCC
GDLLVVQFFAAGMFRHREWIYTALRQQFPGARFHSFADEHVQKQESFDFHSTIDTESAVI
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
TEHGVKFLVDPMGAHKTGFFADQRENRQWLSQGVAGKTVLDLCCNTGGFAVYAALRGAAD
HHCCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHCCCC
VVGVDIDQDVIQIAKANAKLNSVRPKFLQTDIFPYLRDAAVRGDQYDVVILDPAKMTRDR
EEECCCCHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCH
DQIINALKKYLDMNKLALGVVKKGGLFATFSCTGLISEEQFLEMLRRAAFYSGRTIQILK
HHHHHHHHHHHCHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEE
VAGAGPDHPFMAHVQESRYLKATFCRVLD
EECCCCCCCHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA