Click here to switch to the map view.

The map label for this gene is nspVM [H]

Identifier: 182682218

GI number: 182682218

Start: 1874331

End: 1875785

Strand: Reverse

Name: nspVM [H]

Synonym: XfasM23_1700

Alternate gene names: 182682218

Gene position: 1875785-1874331 (Counterclockwise)

Preceding gene: 182682219

Following gene: 182682213

Centisome position: 73.98

GC content: 55.6

Gene sequence:

>1455_bases
GTGAACGAAGCAAAGAAACGCAAAATTGAGTTCGGAGACTTCCAGACTCCGTCTGCACTAGCGCGCCTCGTTTGCGAAAG
ACTGTCCTTGCTAGGCTTTGCGCCGGATGTAGTCATTGAGCCAACCTGCGGTGTTGGGGCGTTCGTGCTTACTGCCGCGC
AACAGTTTCCGCGTGCCGAAGTGCATGGCTACGAAATCAACCCCGCCTACCTTGATGAGCTACGTCAAAGCGTCGAAGGG
CTCGGCGTCACTCGACGCGTGAACCTACACCAGCAGGATTTCTTCGAGACCGATTGGCTGGCCGAGCTGGAAGCGATGCC
GGGCAACGTGCTTGTGCTCGGCAATCCGCCGTGGGTGACCAATGCCGGACAGGGAGCCATAGGCGGCACGAATCTTCCTC
AGAAATCGAATTTCCTAAAGCACAAAGGCTTCGACGCCATCACCGGTAAAGCCAACTTCGATATCTCGGAGTGGATGCTC
ATCGACTTGATGCGGACGCTTGGCGCGCGCGGTGGCGATCTAGCGATGCTTGTTAAAACTACGGTCGCTCGTAAGATCAT
CGCTCACGCGGAACGGATGCAGCTAGGCGTTAGCTACGCCACAATCTTCGGCATTGATGCAAAACGCCATTTCGATGCGT
CGGTAGATGCTTGCCTCTTGGCCATGCGGTTCACGGGCGCCCTATCAGATGCTGGTATCGACTATGACGTTCACCTATCA
CTAGATGGCAACGGCACAACTCGCCGAGTTGGACATCGCCTCGGCCTGACCGTTGGCGATCTAGTCCGGTTCGAGCAGCA
TGCAGATTTGATAGGGAGCAGTCCCCAGAAATGGCGTTCCGGTGTTAAGCACGATGCGTCGTCGGTAATGGAGTTTGATT
CTTCTGACGGTTTGCTTCGCAACGGGCTAGATGAAGAAGTGAGAATCGAGCTCGAATACCTGTATCCGCTGATGAAGGGT
TCTGATATTGGATCGAACCGCGACTGGCGCGGGAAGTTCGTTTTGGTCACGCAACGTAAGCCTGAGGACGAGACCGACGC
CATCCGCGAGCGAGCGCCGCGCACGTGGCAGTACCTTGATGGTCACGGAGACATTCTGGACGCAAGAGGAAGCGCCATCT
ACCGAAAGAATCCGCGCTTCGCGATCTTTGGCATCGGCGACTATGCGTTCCGACCGTGGCGCATTGCGATTTGCGGCCTT
TACAAGAAACTCAACTTTCGACTGGTCGGGCCAATTGAAGGTAAGCCCGTTCAATTCGACGACACCGTTTACTACGTATC
CTTCGATTCGCGCGATGAAGCGCAGGACGCGCTAGAGCGCATTTGTTCCATTGAAGCCACAGAACTGTATTCGTCGCTCA
TCTTCTGGGATGAGAAAAGGCCGATTAAATCCAGCGTGCTCAATGCTGTCGATTGGTCGCGACTGCCTGTTCCGCACAAG
CGCCGCGCCGCATAG

Upstream 100 bases:

>100_bases
TGACCATTTCCCCCGCGCTGCAGTGGCGCCTACAGTACAAGCGCGTCATTCAGAAGGCTGGCGAAGTTGCCGGAGTGCGC
TCGATCTACAAGGCCGGACG

Downstream 100 bases:

>100_bases
GTGACAGCAGCCCCCGGCGTCCCCACCGGCCAGAGGAAGCCTTAGACGCCCGGGGGTGCTGCCGTACAACGCTGTGGCGG
CTACGCGGCCTCGCCCGATG

Product: modification methylase NspV

Products: NA

Alternate protein names: M.NspV; Methyltransferase NspV [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MNEAKKRKIEFGDFQTPSALARLVCERLSLLGFAPDVVIEPTCGVGAFVLTAAQQFPRAEVHGYEINPAYLDELRQSVEG
LGVTRRVNLHQQDFFETDWLAELEAMPGNVLVLGNPPWVTNAGQGAIGGTNLPQKSNFLKHKGFDAITGKANFDISEWML
IDLMRTLGARGGDLAMLVKTTVARKIIAHAERMQLGVSYATIFGIDAKRHFDASVDACLLAMRFTGALSDAGIDYDVHLS
LDGNGTTRRVGHRLGLTVGDLVRFEQHADLIGSSPQKWRSGVKHDASSVMEFDSSDGLLRNGLDEEVRIELEYLYPLMKG
SDIGSNRDWRGKFVLVTQRKPEDETDAIRERAPRTWQYLDGHGDILDARGSAIYRKNPRFAIFGIGDYAFRPWRIAICGL
YKKLNFRLVGPIEGKPVQFDDTVYYVSFDSRDEAQDALERICSIEATELYSSLIFWDEKRPIKSSVLNAVDWSRLPVPHK
RRAA

Sequences:

>Translated_484_residues
MNEAKKRKIEFGDFQTPSALARLVCERLSLLGFAPDVVIEPTCGVGAFVLTAAQQFPRAEVHGYEINPAYLDELRQSVEG
LGVTRRVNLHQQDFFETDWLAELEAMPGNVLVLGNPPWVTNAGQGAIGGTNLPQKSNFLKHKGFDAITGKANFDISEWML
IDLMRTLGARGGDLAMLVKTTVARKIIAHAERMQLGVSYATIFGIDAKRHFDASVDACLLAMRFTGALSDAGIDYDVHLS
LDGNGTTRRVGHRLGLTVGDLVRFEQHADLIGSSPQKWRSGVKHDASSVMEFDSSDGLLRNGLDEEVRIELEYLYPLMKG
SDIGSNRDWRGKFVLVTQRKPEDETDAIRERAPRTWQYLDGHGDILDARGSAIYRKNPRFAIFGIGDYAFRPWRIAICGL
YKKLNFRLVGPIEGKPVQFDDTVYYVSFDSRDEAQDALERICSIEATELYSSLIFWDEKRPIKSSVLNAVDWSRLPVPHK
RRAA
>Mature_484_residues
MNEAKKRKIEFGDFQTPSALARLVCERLSLLGFAPDVVIEPTCGVGAFVLTAAQQFPRAEVHGYEINPAYLDELRQSVEG
LGVTRRVNLHQQDFFETDWLAELEAMPGNVLVLGNPPWVTNAGQGAIGGTNLPQKSNFLKHKGFDAITGKANFDISEWML
IDLMRTLGARGGDLAMLVKTTVARKIIAHAERMQLGVSYATIFGIDAKRHFDASVDACLLAMRFTGALSDAGIDYDVHLS
LDGNGTTRRVGHRLGLTVGDLVRFEQHADLIGSSPQKWRSGVKHDASSVMEFDSSDGLLRNGLDEEVRIELEYLYPLMKG
SDIGSNRDWRGKFVLVTQRKPEDETDAIRERAPRTWQYLDGHGDILDARGSAIYRKNPRFAIFGIGDYAFRPWRIAICGL
YKKLNFRLVGPIEGKPVQFDDTVYYVSFDSRDEAQDALERICSIEATELYSSLIFWDEKRPIKSSVLNAVDWSRLPVPHK
RRAA

Specific function: This methylase recognizes the double-stranded sequence TTCGAA, and methylates it on a yet unknown base to protect it against the NspV endonuclease [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 54125; Mature: 54125

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEAKKRKIEFGDFQTPSALARLVCERLSLLGFAPDVVIEPTCGVGAFVLTAAQQFPRAE
CCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCCC
VHGYEINPAYLDELRQSVEGLGVTRRVNLHQQDFFETDWLAELEAMPGNVLVLGNPPWVT
CCCEECCHHHHHHHHHHHHHCCCEEEECCCHHHCCCHHHHHHHHCCCCCEEEECCCCCCC
NAGQGAIGGTNLPQKSNFLKHKGFDAITGKANFDISEWMLIDLMRTLGARGGDLAMLVKT
CCCCCCCCCCCCCCCCCHHHHCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHH
TVARKIIAHAERMQLGVSYATIFGIDAKRHFDASVDACLLAMRFTGALSDAGIDYDVHLS
HHHHHHHHHHHHHHHCCEEEEEEECCCHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEE
LDGNGTTRRVGHRLGLTVGDLVRFEQHADLIGSSPQKWRSGVKHDASSVMEFDSSDGLLR
ECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHCHHHHHHCCCCCCHHH
NGLDEEVRIELEYLYPLMKGSDIGSNRDWRGKFVLVTQRKPEDETDAIRERAPRTWQYLD
CCCCHHHEEEEHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCEEEEC
GHGDILDARGSAIYRKNPRFAIFGIGDYAFRPWRIAICGLYKKLNFRLVGPIEGKPVQFD
CCCCEEECCCCEEEECCCCEEEEECCCCCCCCCEEHEEHHHHHCCEEEECCCCCCCEEEC
DTVYYVSFDSRDEAQDALERICSIEATELYSSLIFWDEKRPIKSSVLNAVDWSRLPVPHK
CEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHEECCCCCHHHHHHHHHCCCCCCCCCH
RRAA
HCCC
>Mature Secondary Structure
MNEAKKRKIEFGDFQTPSALARLVCERLSLLGFAPDVVIEPTCGVGAFVLTAAQQFPRAE
CCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCCC
VHGYEINPAYLDELRQSVEGLGVTRRVNLHQQDFFETDWLAELEAMPGNVLVLGNPPWVT
CCCEECCHHHHHHHHHHHHHCCCEEEECCCHHHCCCHHHHHHHHCCCCCEEEECCCCCCC
NAGQGAIGGTNLPQKSNFLKHKGFDAITGKANFDISEWMLIDLMRTLGARGGDLAMLVKT
CCCCCCCCCCCCCCCCCHHHHCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHH
TVARKIIAHAERMQLGVSYATIFGIDAKRHFDASVDACLLAMRFTGALSDAGIDYDVHLS
HHHHHHHHHHHHHHHCCEEEEEEECCCHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEE
LDGNGTTRRVGHRLGLTVGDLVRFEQHADLIGSSPQKWRSGVKHDASSVMEFDSSDGLLR
ECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHCCHHHCHHHHHHCCCCCCHHH
NGLDEEVRIELEYLYPLMKGSDIGSNRDWRGKFVLVTQRKPEDETDAIRERAPRTWQYLD
CCCCHHHEEEEHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCEEEEC
GHGDILDARGSAIYRKNPRFAIFGIGDYAFRPWRIAICGLYKKLNFRLVGPIEGKPVQFD
CCCCEEECCCCEEEECCCCEEEEECCCCCCCCCEEHEEHHHHHCCEEEECCCCCCCEEEC
DTVYYVSFDSRDEAQDALERICSIEATELYSSLIFWDEKRPIKSSVLNAVDWSRLPVPHK
CEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHEECCCCCHHHHHHHHHCCCCCCCCCH
RRAA
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8367311 [H]