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The map label for this gene is cbs [H]

Identifier: 182682154

GI number: 182682154

Start: 1812322

End: 1813692

Strand: Reverse

Name: cbs [H]

Synonym: XfasM23_1633

Alternate gene names: 182682154

Gene position: 1813692-1812322 (Counterclockwise)

Preceding gene: 182682155

Following gene: 182682153

Centisome position: 71.53

GC content: 47.7

Gene sequence:

>1371_bases
ATGGTCATTCATTCTTCCGTACTTGAATTGATTGGTAATACGCCGATTATCAAGGCGCAGCGCCTTGACACTGGTCTCTG
TGATTTATTTTTGAAGCTTGAAAGCGCTAATCCTGGTGGTTCAATCAAGGATCGTGTGGGCTTGTCGATGATTGAGGCGG
CTGAGTGCCGTGGTCATATCAAGCCGGGTGCTACGTTGGTTGATGGGGCTGCCAGCAGTACCAGTATGGGCTTGGCTTTT
GTAGCTCGGCAGAAGGGTTATCGCCTGATCCTTGTTGTACCGGACAACATGGCTTGGGAAAAGATTTCTAATCTTAAGGC
TATGGGGGCGGAAGTGATATTGACCCGCTCAGATGTCAGCAAGGGTCATCCAGAGCACTACCAAGATTTGGCTGAAAAAC
TGGCAGCCGAGACACCAGGAGCCTACTTCATTAATCAGTTTGGAAATGCGGACAATCCAGCTGCGCATGAGTTCGGTACT
GGTCCGGAGATTCTGAAGCAGATGGGCGGTCATGTGGATGCGGTTGTATTAGGTTGCGATAGTGCTGGCACCATCACAGG
CTTGTCGCGGGCATTCGACTCAGTTGCTTCTGATGTCGAATTGATTTTGGCCGATCCGGTTGGTTCTATTCTTACCCAGT
ACATCAATGAGGGAACACGTAGTGATAAGTCTGGTACTTGGTTGGTTGAAGGGATTGGAAGTGATTGTTTGCCTCCGATT
TCCGACTTCAGGCGGATCAAAAAAACGTATGCGATCAGTGATGCGGAGAGCTTTCACACTGCACGTGAGTTGTTGAGTAA
AGAGGGGATTCTGGGAGGTTCTTCAACAGGTACTGTGTTGGCTGCGGCATTGAAATACTGCCGTGAGCAGATTGAGCACA
AGCGTGTATTGGTGTTTGTTTGCGACACGGGAAACAAGTATTTGTTAAATATGTATAACGACGATTGGATGTTGGATAAC
GGTTTTTTGAAAAGGCCACATTATGGTGATTTGCGTGATCTGATCCTGCATCCTTACAGTCAGCGTCATACTGTTGTGGT
TGATCCCAATGATTTGCTGACTGTGGCTTACCAGCGTATGAAGCGATACGGTGTTTCACAGTTGCCAGTGATTGAGGACA
GACAGTTGGTCGGTATCCTTGATGAAAGCGATGTGCTGTTACATGTCTATGGTGATGAGACTCGGTTTTGTGACCCTGTT
TCTACCGCGATGGTCAGTAAACTTCACCGATTGGATGTGAAGTCGCCGATTGAGGCGTTACTCCCTGTGTTTGAGCGAGG
CCAAGTGGCTATAGTGATGGAAGATGATCAGTTTTTGGGCCTGATTACACGCATTGATTTATTGAATTACCTGCGTCGTC
GTATCCAATGA

Upstream 100 bases:

>100_bases
GCAGCGAGCGGTGAGTTATTTCCCTGCCGCTCGTTGCTTATACTGCTCTGTTTAGATGAATAATGTGCGACCCTCCTGTA
AGTTCATTGATTGAGAGTAC

Downstream 100 bases:

>100_bases
CGGTTAGATACCTTTGCGCTATAGTGGCTCACTGGCTGCCTGAGTTTTAGTTTTACTGAAGCACAATATTATTCACGACT
ATTCGGTAAGGATTTAGAGC

Product: pyridoxal-5'-phosphate-dependent protein subunit beta

Products: NA

Alternate protein names: Beta-thionase; Serine sulfhydrase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MVIHSSVLELIGNTPIIKAQRLDTGLCDLFLKLESANPGGSIKDRVGLSMIEAAECRGHIKPGATLVDGAASSTSMGLAF
VARQKGYRLILVVPDNMAWEKISNLKAMGAEVILTRSDVSKGHPEHYQDLAEKLAAETPGAYFINQFGNADNPAAHEFGT
GPEILKQMGGHVDAVVLGCDSAGTITGLSRAFDSVASDVELILADPVGSILTQYINEGTRSDKSGTWLVEGIGSDCLPPI
SDFRRIKKTYAISDAESFHTARELLSKEGILGGSSTGTVLAAALKYCREQIEHKRVLVFVCDTGNKYLLNMYNDDWMLDN
GFLKRPHYGDLRDLILHPYSQRHTVVVDPNDLLTVAYQRMKRYGVSQLPVIEDRQLVGILDESDVLLHVYGDETRFCDPV
STAMVSKLHRLDVKSPIEALLPVFERGQVAIVMEDDQFLGLITRIDLLNYLRRRIQ

Sequences:

>Translated_456_residues
MVIHSSVLELIGNTPIIKAQRLDTGLCDLFLKLESANPGGSIKDRVGLSMIEAAECRGHIKPGATLVDGAASSTSMGLAF
VARQKGYRLILVVPDNMAWEKISNLKAMGAEVILTRSDVSKGHPEHYQDLAEKLAAETPGAYFINQFGNADNPAAHEFGT
GPEILKQMGGHVDAVVLGCDSAGTITGLSRAFDSVASDVELILADPVGSILTQYINEGTRSDKSGTWLVEGIGSDCLPPI
SDFRRIKKTYAISDAESFHTARELLSKEGILGGSSTGTVLAAALKYCREQIEHKRVLVFVCDTGNKYLLNMYNDDWMLDN
GFLKRPHYGDLRDLILHPYSQRHTVVVDPNDLLTVAYQRMKRYGVSQLPVIEDRQLVGILDESDVLLHVYGDETRFCDPV
STAMVSKLHRLDVKSPIEALLPVFERGQVAIVMEDDQFLGLITRIDLLNYLRRRIQ
>Mature_456_residues
MVIHSSVLELIGNTPIIKAQRLDTGLCDLFLKLESANPGGSIKDRVGLSMIEAAECRGHIKPGATLVDGAASSTSMGLAF
VARQKGYRLILVVPDNMAWEKISNLKAMGAEVILTRSDVSKGHPEHYQDLAEKLAAETPGAYFINQFGNADNPAAHEFGT
GPEILKQMGGHVDAVVLGCDSAGTITGLSRAFDSVASDVELILADPVGSILTQYINEGTRSDKSGTWLVEGIGSDCLPPI
SDFRRIKKTYAISDAESFHTARELLSKEGILGGSSTGTVLAAALKYCREQIEHKRVLVFVCDTGNKYLLNMYNDDWMLDN
GFLKRPHYGDLRDLILHPYSQRHTVVVDPNDLLTVAYQRMKRYGVSQLPVIEDRQLVGILDESDVLLHVYGDETRFCDPV
STAMVSKLHRLDVKSPIEALLPVFERGQVAIVMEDDQFLGLITRIDLLNYLRRRIQ

Specific function: Two Cysteine Synthase Enzymes Are Found. Both Catalyze The Same Reaction. Cysteine Synthase B Can Also Use Thiosulfate In Place Of Sulfide To Give Cysteine Thiosulfonate As A Product. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=470, Percent_Identity=30.8510638297872, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI295821200, Length=470, Percent_Identity=30.8510638297872, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI4557415, Length=470, Percent_Identity=30.8510638297872, Blast_Score=239, Evalue=4e-63,
Organism=Escherichia coli, GI2367138, Length=306, Percent_Identity=37.2549019607843, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1788754, Length=303, Percent_Identity=36.963696369637, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17534315, Length=330, Percent_Identity=36.6666666666667, Blast_Score=177, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI32566674, Length=301, Percent_Identity=35.2159468438538, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17562970, Length=308, Percent_Identity=34.7402597402597, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17535051, Length=304, Percent_Identity=35.5263157894737, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI115535073, Length=341, Percent_Identity=34.8973607038123, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI25147552, Length=327, Percent_Identity=34.2507645259939, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17561720, Length=291, Percent_Identity=35.3951890034364, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI32566672, Length=224, Percent_Identity=32.5892857142857, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71996324, Length=339, Percent_Identity=27.4336283185841, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17539800, Length=344, Percent_Identity=23.2558139534884, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6321594, Length=505, Percent_Identity=33.2673267326733, Blast_Score=245, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6321449, Length=340, Percent_Identity=32.0588235294118, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24643623, Length=463, Percent_Identity=30.6695464362851, Blast_Score=211, Evalue=1e-54,
Organism=Drosophila melanogaster, GI20129101, Length=463, Percent_Identity=30.6695464362851, Blast_Score=211, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005857
- InterPro:   IPR000644
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00571 CBS; PF00291 PALP [H]

EC number: =4.2.1.22 [H]

Molecular weight: Translated: 50126; Mature: 50126

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIHSSVLELIGNTPIIKAQRLDTGLCDLFLKLESANPGGSIKDRVGLSMIEAAECRGHI
CCCHHHHHHHHCCCCEEEHHHCCCCHHHHEEEEECCCCCCCCHHHHCHHHHHHHHHCCCC
KPGATLVDGAASSTSMGLAFVARQKGYRLILVVPDNMAWEKISNLKAMGAEVILTRSDVS
CCCCEEECCCCCCCCHHHHEEEECCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCC
KGHPEHYQDLAEKLAAETPGAYFINQFGNADNPAAHEFGTGPEILKQMGGHVDAVVLGCD
CCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEECC
SAGTITGLSRAFDSVASDVELILADPVGSILTQYINEGTRSDKSGTWLVEGIGSDCLPPI
CCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCH
SDFRRIKKTYAISDAESFHTARELLSKEGILGGSSTGTVLAAALKYCREQIEHKRVLVFV
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
CDTGNKYLLNMYNDDWMLDNGFLKRPHYGDLRDLILHPYSQRHTVVVDPNDLLTVAYQRM
ECCCCEEEEEEECCCEEECCCCCCCCCCCCHHHHHHCCCCCCCEEEECCCHHHHHHHHHH
KRYGVSQLPVIEDRQLVGILDESDVLLHVYGDETRFCDPVSTAMVSKLHRLDVKSPIEAL
HHCCCCCCCEECCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHH
LPVFERGQVAIVMEDDQFLGLITRIDLLNYLRRRIQ
HHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVIHSSVLELIGNTPIIKAQRLDTGLCDLFLKLESANPGGSIKDRVGLSMIEAAECRGHI
CCCHHHHHHHHCCCCEEEHHHCCCCHHHHEEEEECCCCCCCCHHHHCHHHHHHHHHCCCC
KPGATLVDGAASSTSMGLAFVARQKGYRLILVVPDNMAWEKISNLKAMGAEVILTRSDVS
CCCCEEECCCCCCCCHHHHEEEECCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCC
KGHPEHYQDLAEKLAAETPGAYFINQFGNADNPAAHEFGTGPEILKQMGGHVDAVVLGCD
CCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEECC
SAGTITGLSRAFDSVASDVELILADPVGSILTQYINEGTRSDKSGTWLVEGIGSDCLPPI
CCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCH
SDFRRIKKTYAISDAESFHTARELLSKEGILGGSSTGTVLAAALKYCREQIEHKRVLVFV
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
CDTGNKYLLNMYNDDWMLDNGFLKRPHYGDLRDLILHPYSQRHTVVVDPNDLLTVAYQRM
ECCCCEEEEEEECCCEEECCCCCCCCCCCCHHHHHHCCCCCCCEEEECCCHHHHHHHHHH
KRYGVSQLPVIEDRQLVGILDESDVLLHVYGDETRFCDPVSTAMVSKLHRLDVKSPIEAL
HHCCCCCCCEECCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHH
LPVFERGQVAIVMEDDQFLGLITRIDLLNYLRRRIQ
HHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]