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The map label for this gene is ispH

Identifier: 182682041

GI number: 182682041

Start: 1685989

End: 1686939

Strand: Reverse

Name: ispH

Synonym: XfasM23_1519

Alternate gene names: 182682041

Gene position: 1686939-1685989 (Counterclockwise)

Preceding gene: 182682042

Following gene: 182682040

Centisome position: 66.53

GC content: 58.57

Gene sequence:

>951_bases
ATGAAAGTTCTGCTCGCTAATCCGCGCGGCTTCTGTGCAGGCGTTGATCGCGCCATCGAAATCGTTAAACGCACCATCGA
CATGCTGGGAACCCCCATCTACGTCCGTCATGAAGTCGTCCATAACCGCTTTGTCGTTGATGACCTAAAACAGCGCGGCG
CCATCTTCGTTGAAGAACTCCACCAAGTCCCCGATGGCGCCACCGTCATTTTCAGCGCCCATGGCGTCTCCCAGGCCGTA
CGCCGCCAAGCCGCACAACGCGGCCTAAAAGTCTTTGACGCCACCTGTCCCCTCGTCACCAAAGTCCACCTAGACGTCGC
CCGCCACTGCCGTACTGGACGCGACATGATCCTCATCGGCCATGCCGGACACCCAGAAGTGGAGGGCACCATGGGTCAAT
GGGACCAAGAGCGCGGTACCGGTCGTATCTACCTAGTGGAAAATATCGACGATGTCGCCACCCTCCATGTTGCCCAACCC
CACCATCTGGCCTACACCACCCAAACCACCCTCTCCGTTGACGACACCCGCAACATCATTGACGCCTTACGCCAACGTTT
CCCAACCATCCAAGGACCCAAAAACAATGACATCTGCTACGCCACCCAGAACCGCCAAGATGCAGTTCGCGAACTCGCGC
GCGAATGCGACCTAGTCCTCGTCGTCGGCTCCCCAAACAGCTCCAACTCCAACCGACTCAGCGAACTCGCACAACGCGAA
GGCGTTGCCTCCTATCTCATCGACAGCGCCGCCGAAATTGACCCCGCCTGGGTCATCGACAAACACCACATTGGTGTGAC
CGCCGGCGCCTCCGCCCCTCAAGTCTTGGTTGACGGCGTCCTAGCACGCCTCTACGAACTCGGCGCAACCAGCGTCTCCG
AACACAGCGGCAAACCAGAATCTATGGTCTTTGCCCTCCCCAAAGCACTGCGCCTACAACTCGTGGATTAA

Upstream 100 bases:

>100_bases
ACTGAGCCTATTTGACAGCAAACACTCCCCCAAAACCCCATAATTTCCGTTCCTACCCCCTTCCTGACATCGCGCCCCAC
CATCCCATGGAAACCACGCA

Downstream 100 bases:

>100_bases
ACACACTGAATTGAACACTTGCCGATAGAGCACGGCAGCATAGACCGCCCCCTCCTCATCCACAGCGCCCCTGCCAATGA
CCCCGCCTGAGTGATCAACA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKVLLANPRGFCAGVDRAIEIVKRTIDMLGTPIYVRHEVVHNRFVVDDLKQRGAIFVEELHQVPDGATVIFSAHGVSQAV
RRQAAQRGLKVFDATCPLVTKVHLDVARHCRTGRDMILIGHAGHPEVEGTMGQWDQERGTGRIYLVENIDDVATLHVAQP
HHLAYTTQTTLSVDDTRNIIDALRQRFPTIQGPKNNDICYATQNRQDAVRELARECDLVLVVGSPNSSNSNRLSELAQRE
GVASYLIDSAAEIDPAWVIDKHHIGVTAGASAPQVLVDGVLARLYELGATSVSEHSGKPESMVFALPKALRLQLVD

Sequences:

>Translated_316_residues
MKVLLANPRGFCAGVDRAIEIVKRTIDMLGTPIYVRHEVVHNRFVVDDLKQRGAIFVEELHQVPDGATVIFSAHGVSQAV
RRQAAQRGLKVFDATCPLVTKVHLDVARHCRTGRDMILIGHAGHPEVEGTMGQWDQERGTGRIYLVENIDDVATLHVAQP
HHLAYTTQTTLSVDDTRNIIDALRQRFPTIQGPKNNDICYATQNRQDAVRELARECDLVLVVGSPNSSNSNRLSELAQRE
GVASYLIDSAAEIDPAWVIDKHHIGVTAGASAPQVLVDGVLARLYELGATSVSEHSGKPESMVFALPKALRLQLVD
>Mature_316_residues
MKVLLANPRGFCAGVDRAIEIVKRTIDMLGTPIYVRHEVVHNRFVVDDLKQRGAIFVEELHQVPDGATVIFSAHGVSQAV
RRQAAQRGLKVFDATCPLVTKVHLDVARHCRTGRDMILIGHAGHPEVEGTMGQWDQERGTGRIYLVENIDDVATLHVAQP
HHLAYTTQTTLSVDDTRNIIDALRQRFPTIQGPKNNDICYATQNRQDAVRELARECDLVLVVGSPNSSNSNRLSELAQRE
GVASYLIDSAAEIDPAWVIDKHHIGVTAGASAPQVLVDGVLARLYELGATSVSEHSGKPESMVFALPKALRLQLVD

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=314, Percent_Identity=62.1019108280255, Blast_Score=401, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_XYLF2 (B2I6V0)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830201.1
- GeneID:   6204074
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1519
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- ProtClustDB:   PRK01045
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34705; Mature: 34705

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLLANPRGFCAGVDRAIEIVKRTIDMLGTPIYVRHEVVHNRFVVDDLKQRGAIFVEEL
CEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHH
HQVPDGATVIFSAHGVSQAVRRQAAQRGLKVFDATCPLVTKVHLDVARHCRTGRDMILIG
HHCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEE
HAGHPEVEGTMGQWDQERGTGRIYLVENIDDVATLHVAQPHHLAYTTQTTLSVDDTRNII
CCCCCCCCCCCCCCHHHCCCEEEEEEECCCCEEEEEECCCCEEEEEEEEEEECCHHHHHH
DALRQRFPTIQGPKNNDICYATQNRQDAVRELARECDLVLVVGSPNSSNSNRLSELAQRE
HHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHC
GVASYLIDSAAEIDPAWVIDKHHIGVTAGASAPQVLVDGVLARLYELGATSVSEHSGKPE
CHHHHHHHHHCCCCCEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCC
SMVFALPKALRLQLVD
HHEECCCHHHEEEECC
>Mature Secondary Structure
MKVLLANPRGFCAGVDRAIEIVKRTIDMLGTPIYVRHEVVHNRFVVDDLKQRGAIFVEEL
CEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHH
HQVPDGATVIFSAHGVSQAVRRQAAQRGLKVFDATCPLVTKVHLDVARHCRTGRDMILIG
HHCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEE
HAGHPEVEGTMGQWDQERGTGRIYLVENIDDVATLHVAQPHHLAYTTQTTLSVDDTRNII
CCCCCCCCCCCCCCHHHCCCEEEEEEECCCCEEEEEECCCCEEEEEEEEEEECCHHHHHH
DALRQRFPTIQGPKNNDICYATQNRQDAVRELARECDLVLVVGSPNSSNSNRLSELAQRE
HHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHC
GVASYLIDSAAEIDPAWVIDKHHIGVTAGASAPQVLVDGVLARLYELGATSVSEHSGKPE
CHHHHHHHHHCCCCCEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCC
SMVFALPKALRLQLVD
HHEECCCHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA