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The map label for this gene is ybjS [C]

Identifier: 182681647

GI number: 182681647

Start: 1250555

End: 1251553

Strand: Direct

Name: ybjS [C]

Synonym: XfasM23_1105

Alternate gene names: 182681647

Gene position: 1250555-1251553 (Clockwise)

Preceding gene: 182681646

Following gene: 182681648

Centisome position: 49.32

GC content: 52.65

Gene sequence:

>999_bases
ATGAGAATCCTCGTTACCGGAGGGAGTGGGTTCCTCGGCGAAGCACTATGCCGAGGATTACTTAAGCGTGGCTACCAAGT
AGTTAGTTTCCAACGTAGTCACTACCAAGCACTGCAAGCACTAGGCGTTGTGCAGATTTGCGGAGATCTGAGCGATTTTC
ACGCGGTGCGGCACGCGGTGCGTGGTGTGGATGCGGTTTTCCATAATGCTGCCAAGGTAGGTGCATGGGGTTCCTACACT
AGTTACCACCAAATCAATGTGATCGGCACACAACACGTTCTGGATGCCTGTCGTGCTGAGAACATTAACAAGTTAGTGTA
CACCTCAACCCCGAGTGTGATTCATCGCTCAAACTACCCGGTTGAGGGTTTAGATGCAGATCAAGTGCCATATAGCAACG
CCGTTAAAGTGCCATACGCTGCGACTAAAGCAATGGCTGAACAGGCGGTGTTAGCAGCCAACAGTGTCGACTTAACCACC
GTAGCACTACGCCCACGCATGATATGGGGCCCAGGCGACCCTCACTTGATGCCTCGCTTAGTGGCGCGCGCCCGCGCTGG
GCGACTGCGTCTGATAGGCGATGGTCGTAACTTAGTAGATAGCACCTACATTGACAATGCCGCACAAGCACATTTCGATG
CGTTTGAGCATCTCATGCCTGGAGCAGCGTGTGCTGGCAAGGCTTATTTCATCTCCAACGGCGAGCCGTTGCAGATGCGC
GAATTGATCAACAAGCTACTGGCCACTACCAACGCTCCACCGGTGACTCAATCACTGTCATTTAAGACGGGTTACTGCAT
TGGTGCATTTTGCGAGATGCTATGGTCGTTACTACCCCTCCCTGGAGAACCACTACTAACGCGTTTTCTCGTTGAGCAGA
TGTCTACTCCTCATTGGTACAGCATAGAACCAGCGAAGCGTGACTTCGGGTACGTGCCGCGCGTGTCCATTGAAGAGGGC
TTGGTACGTTTGCTGTCCGAAACGCGTGTTACTTGTTGA

Upstream 100 bases:

>100_bases
CGTGTTGGTGCTGGTATATGAAGGTGGCAGCACTACAGTCAGTTTGGCCACAGCAGGTTAATCTTCCCGCCTCTCCACTG
ATAATAATGCTGACATTTTT

Downstream 100 bases:

>100_bases
TGTATCTGTGCAGCAATACCAGCAACTTCATCTCTGGGGTTTCTATTGAACTAACTTGATAGCAGGCGTAAAAATGGCCG
TTCTGTTTTCATTCGGTCAA

Product: 3-beta hydroxysteroid dehydrogenase/isomerase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MRILVTGGSGFLGEALCRGLLKRGYQVVSFQRSHYQALQALGVVQICGDLSDFHAVRHAVRGVDAVFHNAAKVGAWGSYT
SYHQINVIGTQHVLDACRAENINKLVYTSTPSVIHRSNYPVEGLDADQVPYSNAVKVPYAATKAMAEQAVLAANSVDLTT
VALRPRMIWGPGDPHLMPRLVARARAGRLRLIGDGRNLVDSTYIDNAAQAHFDAFEHLMPGAACAGKAYFISNGEPLQMR
ELINKLLATTNAPPVTQSLSFKTGYCIGAFCEMLWSLLPLPGEPLLTRFLVEQMSTPHWYSIEPAKRDFGYVPRVSIEEG
LVRLLSETRVTC

Sequences:

>Translated_332_residues
MRILVTGGSGFLGEALCRGLLKRGYQVVSFQRSHYQALQALGVVQICGDLSDFHAVRHAVRGVDAVFHNAAKVGAWGSYT
SYHQINVIGTQHVLDACRAENINKLVYTSTPSVIHRSNYPVEGLDADQVPYSNAVKVPYAATKAMAEQAVLAANSVDLTT
VALRPRMIWGPGDPHLMPRLVARARAGRLRLIGDGRNLVDSTYIDNAAQAHFDAFEHLMPGAACAGKAYFISNGEPLQMR
ELINKLLATTNAPPVTQSLSFKTGYCIGAFCEMLWSLLPLPGEPLLTRFLVEQMSTPHWYSIEPAKRDFGYVPRVSIEEG
LVRLLSETRVTC
>Mature_332_residues
MRILVTGGSGFLGEALCRGLLKRGYQVVSFQRSHYQALQALGVVQICGDLSDFHAVRHAVRGVDAVFHNAAKVGAWGSYT
SYHQINVIGTQHVLDACRAENINKLVYTSTPSVIHRSNYPVEGLDADQVPYSNAVKVPYAATKAMAEQAVLAANSVDLTT
VALRPRMIWGPGDPHLMPRLVARARAGRLRLIGDGRNLVDSTYIDNAAQAHFDAFEHLMPGAACAGKAYFISNGEPLQMR
ELINKLLATTNAPPVTQSLSFKTGYCIGAFCEMLWSLLPLPGEPLLTRFLVEQMSTPHWYSIEPAKRDFGYVPRVSIEEG
LVRLLSETRVTC

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=339, Percent_Identity=32.7433628318584, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI8393516, Length=339, Percent_Identity=32.7433628318584, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI116268111, Length=329, Percent_Identity=33.1306990881459, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI310132178, Length=332, Percent_Identity=33.1325301204819, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI310113012, Length=332, Percent_Identity=33.1325301204819, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI239745448, Length=332, Percent_Identity=32.8313253012048, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI260763931, Length=344, Percent_Identity=29.3604651162791, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI4504509, Length=344, Percent_Identity=29.3604651162791, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI4504507, Length=344, Percent_Identity=29.9418604651163, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI19923621, Length=355, Percent_Identity=32.112676056338, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI218563686, Length=163, Percent_Identity=36.8098159509202, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI218563684, Length=163, Percent_Identity=36.8098159509202, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI87081792, Length=334, Percent_Identity=25.4491017964072, Blast_Score=98, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17570557, Length=331, Percent_Identity=29.0030211480363, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI193211272, Length=345, Percent_Identity=24.3478260869565, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321437, Length=343, Percent_Identity=32.9446064139942, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21355349, Length=277, Percent_Identity=28.8808664259928, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 36382; Mature: 36382

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVTGGSGFLGEALCRGLLKRGYQVVSFQRSHYQALQALGVVQICGDLSDFHAVRHAV
CEEEEECCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
RGVDAVFHNAAKVGAWGSYTSYHQINVIGTQHVLDACRAENINKLVYTSTPSVIHRSNYP
HHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHHHHHHCCCCEEEEECCCCCEECCCCC
VEGLDADQVPYSNAVKVPYAATKAMAEQAVLAANSVDLTTVALRPRMIWGPGDPHLMPRL
CCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCHHHHHH
VARARAGRLRLIGDGRNLVDSTYIDNAAQAHFDAFEHLMPGAACAGKAYFISNGEPLQMR
HHHHCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCEEEECCCCCHHHH
ELINKLLATTNAPPVTQSLSFKTGYCIGAFCEMLWSLLPLPGEPLLTRFLVEQMSTPHWY
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEE
SIEPAKRDFGYVPRVSIEEGLVRLLSETRVTC
ECCCCHHCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRILVTGGSGFLGEALCRGLLKRGYQVVSFQRSHYQALQALGVVQICGDLSDFHAVRHAV
CEEEEECCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
RGVDAVFHNAAKVGAWGSYTSYHQINVIGTQHVLDACRAENINKLVYTSTPSVIHRSNYP
HHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHHHHHHCCCCEEEEECCCCCEECCCCC
VEGLDADQVPYSNAVKVPYAATKAMAEQAVLAANSVDLTTVALRPRMIWGPGDPHLMPRL
CCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCHHHHHH
VARARAGRLRLIGDGRNLVDSTYIDNAAQAHFDAFEHLMPGAACAGKAYFISNGEPLQMR
HHHHCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCEEEECCCCCHHHH
ELINKLLATTNAPPVTQSLSFKTGYCIGAFCEMLWSLLPLPGEPLLTRFLVEQMSTPHWY
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEE
SIEPAKRDFGYVPRVSIEEGLVRLLSETRVTC
ECCCCHHCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]