Click here to switch to the map view.

The map label for this gene is topB [H]

Identifier: 182681629

GI number: 182681629

Start: 1230941

End: 1231888

Strand: Direct

Name: topB [H]

Synonym: XfasM23_1087

Alternate gene names: 182681629

Gene position: 1230941-1231888 (Clockwise)

Preceding gene: 182681628

Following gene: 182681633

Centisome position: 48.54

GC content: 51.9

Gene sequence:

>948_bases
GTGACAGGGAAGAGTGAGGGTTACATTGAATGCGGTCACGATACGATCACTTGGTGTTTCGGACACATGCTCGAACAAGC
TGAGCCAGATGCGTACATTTCTGAAGACGTTCCACTGAATCCAAAAACTGGTAAGAAAATCTGGCGCGTCGAAGAACTAC
CTATCATTCCTGAAACGTGGATCATGCAGCCCCGCGAAGACGCGAAAGCGCAGCTTGAGGTCATCAAGCGGCTGCTGAAA
AAGGCAACTGAAATTGTCAATGCTGGTGACCCAGACCGTGAGGGGCAGTTACTCGTTGATGAAGTGCTGGAATACTTCGA
CTGCCACAAGTCGGTGCAACGCTTCTGGGTATCGGCACAAGATTCCGTATCGGTGCAACTTGACCTTGCTGCGCTCAGAA
GTAACACCGAGTATCAAGGCTTTGCCAATGCAGCACGGGCACGTAGTCGGGCCGATTGGCTGATTGGTATCAATTTCAGC
AGGGCTTATACGCTACGCGCACAGCGCGGGGGTTCGCGTGCATTGCTGACGATAGGGCGTGTACAGACTCCAACGCTGGC
GCTGGTGGTCATGCGTGATCGTGAGAGTGAGGCATTCAAATCGATTCCTTACCACACGATCCGTGCAGCATTTAAACACG
AGGGTGGTGTATTCGTTGCCGTTTGGCGGGCGAAAGAAGATCAAGTCGGGCTAGACCCAGAGGGTCGTTTGGTCGACACG
GTCATCGCGAACACGTTGGTCGAATCCATCACCGGTCATATAGGTAAGGTCATCAAGTACAAGCAGGAGCCAAAGAAGAA
GGCACAGCCGCGTGCTTATTCACTTTCTGAGATTACTCTCTTGGCGTCTAACAAATTCGGGTATAGCGCGGCAGATGTGC
TTGAGGTGTGCCAATCGCTTTATGAGACACATAAACTCACGAGCTACCCAAGAACCGATTGTGGGTAG

Upstream 100 bases:

>100_bases
CCAGCTTCGGCCAGCGCGGCTTGATAAACCCGGTCGACGCATCGGCGGGCATCTTGCAGAGAAGCCATCTGTTGCCAAAG
CAATCGCCGGTGAGCTGGGT

Downstream 100 bases:

>100_bases
CTGCCGGAATCACAACCATCTAATCACTGCCACCATTGCAATGGTAGATTCAATAAGTAACGTTATGGGGATGACCATCA
CATGGACTTCGTGGCACGGT

Product: DNA topoisomerase

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTGKSEGYIECGHDTITWCFGHMLEQAEPDAYISEDVPLNPKTGKKIWRVEELPIIPETWIMQPREDAKAQLEVIKRLLK
KATEIVNAGDPDREGQLLVDEVLEYFDCHKSVQRFWVSAQDSVSVQLDLAALRSNTEYQGFANAARARSRADWLIGINFS
RAYTLRAQRGGSRALLTIGRVQTPTLALVVMRDRESEAFKSIPYHTIRAAFKHEGGVFVAVWRAKEDQVGLDPEGRLVDT
VIANTLVESITGHIGKVIKYKQEPKKKAQPRAYSLSEITLLASNKFGYSAADVLEVCQSLYETHKLTSYPRTDCG

Sequences:

>Translated_315_residues
MTGKSEGYIECGHDTITWCFGHMLEQAEPDAYISEDVPLNPKTGKKIWRVEELPIIPETWIMQPREDAKAQLEVIKRLLK
KATEIVNAGDPDREGQLLVDEVLEYFDCHKSVQRFWVSAQDSVSVQLDLAALRSNTEYQGFANAARARSRADWLIGINFS
RAYTLRAQRGGSRALLTIGRVQTPTLALVVMRDRESEAFKSIPYHTIRAAFKHEGGVFVAVWRAKEDQVGLDPEGRLVDT
VIANTLVESITGHIGKVIKYKQEPKKKAQPRAYSLSEITLLASNKFGYSAADVLEVCQSLYETHKLTSYPRTDCG
>Mature_314_residues
TGKSEGYIECGHDTITWCFGHMLEQAEPDAYISEDVPLNPKTGKKIWRVEELPIIPETWIMQPREDAKAQLEVIKRLLKK
ATEIVNAGDPDREGQLLVDEVLEYFDCHKSVQRFWVSAQDSVSVQLDLAALRSNTEYQGFANAARARSRADWLIGINFSR
AYTLRAQRGGSRALLTIGRVQTPTLALVVMRDRESEAFKSIPYHTIRAAFKHEGGVFVAVWRAKEDQVGLDPEGRLVDTV
IANTLVESITGHIGKVIKYKQEPKKKAQPRAYSLSEITLLASNKFGYSAADVLEVCQSLYETHKLTSYPRTDCG

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=249, Percent_Identity=26.9076305220884, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788061, Length=318, Percent_Identity=44.0251572327044, Blast_Score=268, Evalue=5e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 35402; Mature: 35270

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKSEGYIECGHDTITWCFGHMLEQAEPDAYISEDVPLNPKTGKKIWRVEELPIIPETW
CCCCCCCCEEECCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEEECCCCCCHHH
IMQPREDAKAQLEVIKRLLKKATEIVNAGDPDREGQLLVDEVLEYFDCHKSVQRFWVSAQ
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
DSVSVQLDLAALRSNTEYQGFANAARARSRADWLIGINFSRAYTLRAQRGGSRALLTIGR
CCEEEEEEHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCEEEEEECC
VQTPTLALVVMRDRESEAFKSIPYHTIRAAFKHEGGVFVAVWRAKEDQVGLDPEGRLVDT
CCCCEEEEEEEECCCHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHH
VIANTLVESITGHIGKVIKYKQEPKKKAQPRAYSLSEITLLASNKFGYSAADVLEVCQSL
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHEEEEEECCCCCCCHHHHHHHHHHH
YETHKLTSYPRTDCG
HHHHHCCCCCCCCCC
>Mature Secondary Structure 
TGKSEGYIECGHDTITWCFGHMLEQAEPDAYISEDVPLNPKTGKKIWRVEELPIIPETW
CCCCCCCEEECCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEEECCCCCCHHH
IMQPREDAKAQLEVIKRLLKKATEIVNAGDPDREGQLLVDEVLEYFDCHKSVQRFWVSAQ
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
DSVSVQLDLAALRSNTEYQGFANAARARSRADWLIGINFSRAYTLRAQRGGSRALLTIGR
CCEEEEEEHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCEEEEEECC
VQTPTLALVVMRDRESEAFKSIPYHTIRAAFKHEGGVFVAVWRAKEDQVGLDPEGRLVDT
CCCCEEEEEEEECCCHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHH
VIANTLVESITGHIGKVIKYKQEPKKKAQPRAYSLSEITLLASNKFGYSAADVLEVCQSL
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHEEEEEECCCCCCCHHHHHHHHHHH
YETHKLTSYPRTDCG
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2553698; 9097039; 9278503; 10574789 [H]