Click here to switch to the map view.

The map label for this gene is xthA [H]

Identifier: 182681462

GI number: 182681462

Start: 1084607

End: 1085401

Strand: Reverse

Name: xthA [H]

Synonym: XfasM23_0913

Alternate gene names: 182681462

Gene position: 1085401-1084607 (Counterclockwise)

Preceding gene: 182681463

Following gene: 182681461

Centisome position: 42.8

GC content: 53.58

Gene sequence:

>795_bases
ATGAAAATGCGTATTGCAAGTTGGAACGTTAATTCACTCAATGTACGTTTGTCTCAGTTGCAGCAGTGGCTGGTGGAGGC
TGCGCCTGATGTGCTTGGCCTTCAGGAAACGAAGTTGGAGGACCATCAGTTTCCAGAAGCAGCGTTGGCGGCATTGGGCT
ATGTCAGTGTGTATGCGGGGCAGAAGACGTATAACGGTGTCGCACTTCTGGCACGCGTGGCGTTGGAGGATGTGCAAGTC
GGTATCCCGGAGTTCGAGGATGTGCAGCGACGGGTGATCGCTGCAACTGTGGATGGGATACGCGTTGTCAATCTATACGT
GGTTAATGGTCAAGAGGTTGGGACAGACAAGTACGCTTACAAATTAAGCTGGTTAGATGCGGTGTACCACTGGCTGGCCG
AGGAGCTGCAACGCTATCCCCGCCTTGTGGTTCTGGGTGATTTCAATATTGCTCCTGATGCCCGGGACGTCTACGATCCG
CAGGTATGGAATGAGGATCATATTCTCACTTCGACCACGGAACGTGCGGCACTGGATAAGCTGTTAGGACTGGGGTTGCA
TGATGGTTTTCGCTTGCATCACCAGGATGCGGGGATATTCAGTTGGTGGGATTACCGGCAAGCTAGTTTCCGGCGCAATA
GGGGCTTACGTATTGATCTCACGCTTGTTTCGGAGGCGTTGCGCGCGCAGGCGGTGGCTTCTGGAATCGACCGCGTGCCT
AGAGGCTGGGAACGACCCAGTGATCATGCTCCAGCGTGGATTGAATTAGCTTTGGCGGTGTCTTCATCACCTTAG

Upstream 100 bases:

>100_bases
GGGATCAGTAAGGGTTACTGCGCCTAGGGTTGTAGGTAGGCCAAGTAGATAAGAATTACCTGGGATGTGGGCTGTTTTTA
TATGAAATCAATTTTTTAAT

Downstream 100 bases:

>100_bases
GCGTTCGGTGTTGAGAGACCGCCCGAAAACGTCAATGGGAGGTGTGTTATCTCACGCAATAACGACCACGTTTTGATGTC
ATGCAAGTGCGGTAAGTACG

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKMRIASWNVNSLNVRLSQLQQWLVEAAPDVLGLQETKLEDHQFPEAALAALGYVSVYAGQKTYNGVALLARVALEDVQV
GIPEFEDVQRRVIAATVDGIRVVNLYVVNGQEVGTDKYAYKLSWLDAVYHWLAEELQRYPRLVVLGDFNIAPDARDVYDP
QVWNEDHILTSTTERAALDKLLGLGLHDGFRLHHQDAGIFSWWDYRQASFRRNRGLRIDLTLVSEALRAQAVASGIDRVP
RGWERPSDHAPAWIELALAVSSSP

Sequences:

>Translated_264_residues
MKMRIASWNVNSLNVRLSQLQQWLVEAAPDVLGLQETKLEDHQFPEAALAALGYVSVYAGQKTYNGVALLARVALEDVQV
GIPEFEDVQRRVIAATVDGIRVVNLYVVNGQEVGTDKYAYKLSWLDAVYHWLAEELQRYPRLVVLGDFNIAPDARDVYDP
QVWNEDHILTSTTERAALDKLLGLGLHDGFRLHHQDAGIFSWWDYRQASFRRNRGLRIDLTLVSEALRAQAVASGIDRVP
RGWERPSDHAPAWIELALAVSSSP
>Mature_264_residues
MKMRIASWNVNSLNVRLSQLQQWLVEAAPDVLGLQETKLEDHQFPEAALAALGYVSVYAGQKTYNGVALLARVALEDVQV
GIPEFEDVQRRVIAATVDGIRVVNLYVVNGQEVGTDKYAYKLSWLDAVYHWLAEELQRYPRLVVLGDFNIAPDARDVYDP
QVWNEDHILTSTTERAALDKLLGLGLHDGFRLHHQDAGIFSWWDYRQASFRRNRGLRIDLTLVSEALRAQAVASGIDRVP
RGWERPSDHAPAWIELALAVSSSP

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=264, Percent_Identity=32.1969696969697, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI18375503, Length=264, Percent_Identity=32.1969696969697, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI18375501, Length=264, Percent_Identity=32.1969696969697, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1788046, Length=263, Percent_Identity=38.4030418250951, Blast_Score=161, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI71989536, Length=263, Percent_Identity=28.8973384030418, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221330655, Length=261, Percent_Identity=30.6513409961686, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI17136678, Length=261, Percent_Identity=30.6513409961686, Blast_Score=91, Evalue=6e-19,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29742; Mature: 29742

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMRIASWNVNSLNVRLSQLQQWLVEAAPDVLGLQETKLEDHQFPEAALAALGYVSVYAG
CCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHHHHEECC
QKTYNGVALLARVALEDVQVGIPEFEDVQRRVIAATVDGIRVVNLYVVNGQEVGTDKYAY
CCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHEE
KLSWLDAVYHWLAEELQRYPRLVVLGDFNIAPDARDVYDPQVWNEDHILTSTTERAALDK
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHH
LLGLGLHDGFRLHHQDAGIFSWWDYRQASFRRNRGLRIDLTLVSEALRAQAVASGIDRVP
HHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCC
RGWERPSDHAPAWIELALAVSSSP
CCCCCCCCCCCCEEEEEEEECCCC
>Mature Secondary Structure
MKMRIASWNVNSLNVRLSQLQQWLVEAAPDVLGLQETKLEDHQFPEAALAALGYVSVYAG
CCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCHHHHHHHHHHHEECC
QKTYNGVALLARVALEDVQVGIPEFEDVQRRVIAATVDGIRVVNLYVVNGQEVGTDKYAY
CCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCHHEE
KLSWLDAVYHWLAEELQRYPRLVVLGDFNIAPDARDVYDPQVWNEDHILTSTTERAALDK
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHH
LLGLGLHDGFRLHHQDAGIFSWWDYRQASFRRNRGLRIDLTLVSEALRAQAVASGIDRVP
HHHCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCC
RGWERPSDHAPAWIELALAVSSSP
CCCCCCCCCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]