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The map label for this gene is fis

Identifier: 182681409

GI number: 182681409

Start: 1008784

End: 1009056

Strand: Reverse

Name: fis

Synonym: XfasM23_0858

Alternate gene names: 182681409

Gene position: 1009056-1008784 (Counterclockwise)

Preceding gene: 182681410

Following gene: 182681406

Centisome position: 39.79

GC content: 51.28

Gene sequence:

>273_bases
TTGAACGTAGTACCCTCCCGTCCCGAAGTCAGCCGTGGCTCCCCAAAGTCCCCCCTACGTGAACATGTAGCTCAAGTGGT
GCGCCGTTATTTGCGTGATCTTGATGGCTGCGATGCCAATGATCTCTACAACATGGTGCTGCGCGAAATGGAGATTCCTC
TCTTGGTAGAAGTACTGAACCATTGTGAAGGGAATCAGAGCCGTGCTGCTGCACTGCTTGGCATTCATCGCGCCACACTA
CGTAAGAAACTCAAAGAGTACGGATTAGTTTGA

Upstream 100 bases:

>100_bases
TTTGAATTTCGCTAAAACACACGTGGCATAAGCAACGGTCACGCGCTAGACTTGCCCTCCGCAAAGCGCCTGCCAGACGT
CCAAAGCCCGGAAATTTCCG

Downstream 100 bases:

>100_bases
GGTGGTTCTGGATACGATGTTCAAAACTGTAAAGATACACATGCAGCTCTGTACCATCGGTTCGTTATGTTTCTCCATCG
AACAAAGACGGGAGCAGCAG

Product: DNA-binding protein Fis

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 90

Protein sequence:

>90_residues
MNVVPSRPEVSRGSPKSPLREHVAQVVRRYLRDLDGCDANDLYNMVLREMEIPLLVEVLNHCEGNQSRAAALLGIHRATL
RKKLKEYGLV

Sequences:

>Translated_90_residues
MNVVPSRPEVSRGSPKSPLREHVAQVVRRYLRDLDGCDANDLYNMVLREMEIPLLVEVLNHCEGNQSRAAALLGIHRATL
RKKLKEYGLV
>Mature_90_residues
MNVVPSRPEVSRGSPKSPLREHVAQVVRRYLRDLDGCDANDLYNMVLREMEIPLLVEVLNHCEGNQSRAAALLGIHRATL
RKKLKEYGLV

Specific function: Activates Ribosomal RNA Transcription. Plays A Direct Role In Upstream Activation Of rRNA Promoters. Binds To A Recombinational Enhancer Sequence That Is Required To Stimulate Hin-Mediated DNA Inversion. Prevents Initiation Of DNA Replication From Oric.

COG id: COG2901

COG function: function code KL; Factor for inversion stimulation Fis, transcriptional activator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transcriptional regulatory fis family [H]

Homologues:

Organism=Escherichia coli, GI1789661, Length=89, Percent_Identity=47.1910112359551, Blast_Score=88, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005412
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197 [H]

Pfam domain/function: PF02954 HTH_8 [H]

EC number: NA

Molecular weight: Translated: 10207; Mature: 10207

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVPSRPEVSRGSPKSPLREHVAQVVRRYLRDLDGCDANDLYNMVLREMEIPLLVEVLN
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHH
HCEGNQSRAAALLGIHRATLRKKLKEYGLV
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNVVPSRPEVSRGSPKSPLREHVAQVVRRYLRDLDGCDANDLYNMVLREMEIPLLVEVLN
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHH
HCEGNQSRAAALLGIHRATLRKKLKEYGLV
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]