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The map label for this gene is sucB [H]

Identifier: 182681355

GI number: 182681355

Start: 945765

End: 946940

Strand: Reverse

Name: sucB [H]

Synonym: XfasM23_0802

Alternate gene names: 182681355

Gene position: 946940-945765 (Counterclockwise)

Preceding gene: 182681356

Following gene: 182681354

Centisome position: 37.34

GC content: 51.45

Gene sequence:

>1176_bases
ATGAGCACCGAAGTTAAAGTCCCGGTATTACCTGAATCCGTTTCAGACGCCACCATTGCCAGCTGGCACAAAAAAGCCGG
TGAGATTGTCAAACGCGATGAGAACATCGTCGACCTGGAGACCGACAAAGTTGTATTGGAAGTCCCCTCCCCTGTCGACG
GCGTCTTGAAAGAAATCAAATTCGACGCCGGCAGTACTGTCACCAGTAATCAAGTTCTGGCAATCATTGAAGAAGGCAGC
ATCGTTACTGCACCCTCTCCAGCACCCTCTCAGGTGATTGATCAGAAACCGGTTGCTGTATCCGCTCCAGCCGCCAAATC
CAACGTTGACAGTCTGCCTCCAGGTGCTCGTTTCACGGCAACGACCGAAGGTATTGACCCAGCCCAGATCGAAGGAAGTG
GGCGCCGTGGTGCTGTCACCAAAGAAGATATCATCAACTTTGCTAAGCAAAATGGAGCCGCCCGCGCCAGCGGTACGCGT
TTAGAAGAACGCATCCCAATGACCCGTATCCGGCAACGCATCGCCGAACGCCTCATGCAGTCGAAGAACTCCACTGCGAT
GCTGACCACCTTTAATGAAATCAACCTGGCCAAAGTCTCAAACATACGCAAAGAGCTACAGGAAGAATTCCAGAAAGCCC
ACGGTATCAAATTGGGCTTCATGAGCTTTTTCGTGAAAGCCGCCGCCAATGCACTACAGCGCTTCCCCTTGGTCAACGCA
TCAATTGACGGTACAGACATCATTTACCACGGATATAGCGATATTTCGATCGCGGTATCCACCGATAAAGGCTTAGTCAC
GCCAGTACTGCGCAACGTCGAACGGATGTCTTTCGCCGACATTGAGCATCGCATCGCCGATTACGCCAAGAAGGCACGCG
ACGGCAAATTAAGTCTAGAAGAGTTGCAGGGAGGCACCTTCACCGTCACCAACGGTGGCACCTTTGGCTCATTGCTCTCG
ACTCCAATCGTTAACCCGCCACAAAGCGCGATTTTAGGGATGCATACCATCAAAGAGCGCCCCATTGCTGAGAACGGCAA
CATTGTGATTGCGCCAATGATGTACGTGGCACTGTCATATGATCACCGCATCATTGATGGCAAAGATTCGGTTCAATTCT
TGGTGGACATCAAAAATCAATTGGAAGACCCCGGGCGGATGCTGTTCGGCTTATAA

Upstream 100 bases:

>100_bases
AAGAAAGCCTCGTTGCCGATGCACTGTTGAATCCGTTCAACAATCATGTCGCTGAATAATCATTCTTCACAAGAACAAGA
ATCCCTAGGACACATCCAGA

Downstream 100 bases:

>100_bases
GGAAGAGATTCACACATGGATGCAACATTGATCGCTCTTATAAGTACGACAGTCATCGCGATAGCAACCGTCGTCGCCGC
AATCATCAATACCTTTGGTC

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSTEVKVPVLPESVSDATIASWHKKAGEIVKRDENIVDLETDKVVLEVPSPVDGVLKEIKFDAGSTVTSNQVLAIIEEGS
IVTAPSPAPSQVIDQKPVAVSAPAAKSNVDSLPPGARFTATTEGIDPAQIEGSGRRGAVTKEDIINFAKQNGAARASGTR
LEERIPMTRIRQRIAERLMQSKNSTAMLTTFNEINLAKVSNIRKELQEEFQKAHGIKLGFMSFFVKAAANALQRFPLVNA
SIDGTDIIYHGYSDISIAVSTDKGLVTPVLRNVERMSFADIEHRIADYAKKARDGKLSLEELQGGTFTVTNGGTFGSLLS
TPIVNPPQSAILGMHTIKERPIAENGNIVIAPMMYVALSYDHRIIDGKDSVQFLVDIKNQLEDPGRMLFGL

Sequences:

>Translated_391_residues
MSTEVKVPVLPESVSDATIASWHKKAGEIVKRDENIVDLETDKVVLEVPSPVDGVLKEIKFDAGSTVTSNQVLAIIEEGS
IVTAPSPAPSQVIDQKPVAVSAPAAKSNVDSLPPGARFTATTEGIDPAQIEGSGRRGAVTKEDIINFAKQNGAARASGTR
LEERIPMTRIRQRIAERLMQSKNSTAMLTTFNEINLAKVSNIRKELQEEFQKAHGIKLGFMSFFVKAAANALQRFPLVNA
SIDGTDIIYHGYSDISIAVSTDKGLVTPVLRNVERMSFADIEHRIADYAKKARDGKLSLEELQGGTFTVTNGGTFGSLLS
TPIVNPPQSAILGMHTIKERPIAENGNIVIAPMMYVALSYDHRIIDGKDSVQFLVDIKNQLEDPGRMLFGL
>Mature_390_residues
STEVKVPVLPESVSDATIASWHKKAGEIVKRDENIVDLETDKVVLEVPSPVDGVLKEIKFDAGSTVTSNQVLAIIEEGSI
VTAPSPAPSQVIDQKPVAVSAPAAKSNVDSLPPGARFTATTEGIDPAQIEGSGRRGAVTKEDIINFAKQNGAARASGTRL
EERIPMTRIRQRIAERLMQSKNSTAMLTTFNEINLAKVSNIRKELQEEFQKAHGIKLGFMSFFVKAAANALQRFPLVNAS
IDGTDIIYHGYSDISIAVSTDKGLVTPVLRNVERMSFADIEHRIADYAKKARDGKLSLEELQGGTFTVTNGGTFGSLLST
PIVNPPQSAILGMHTIKERPIAENGNIVIAPMMYVALSYDHRIIDGKDSVQFLVDIKNQLEDPGRMLFGL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=236, Percent_Identity=56.3559322033898, Blast_Score=281, Evalue=5e-76,
Organism=Homo sapiens, GI31711992, Length=430, Percent_Identity=31.1627906976744, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI110671329, Length=425, Percent_Identity=28.7058823529412, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI203098753, Length=452, Percent_Identity=29.646017699115, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI203098816, Length=452, Percent_Identity=29.646017699115, Blast_Score=161, Evalue=8e-40,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=37.888198757764, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI1786946, Length=401, Percent_Identity=53.3665835411471, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1786305, Length=404, Percent_Identity=28.7128712871287, Blast_Score=165, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=407, Percent_Identity=42.7518427518428, Blast_Score=307, Evalue=6e-84,
Organism=Caenorhabditis elegans, GI17560088, Length=430, Percent_Identity=31.6279069767442, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17537937, Length=417, Percent_Identity=26.8585131894484, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=226, Percent_Identity=32.7433628318584, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=401, Percent_Identity=42.3940149625935, Blast_Score=315, Evalue=5e-87,
Organism=Saccharomyces cerevisiae, GI6324258, Length=451, Percent_Identity=26.8292682926829, Blast_Score=145, Evalue=8e-36,
Organism=Drosophila melanogaster, GI24645909, Length=241, Percent_Identity=56.0165975103734, Blast_Score=287, Evalue=8e-78,
Organism=Drosophila melanogaster, GI24582497, Length=230, Percent_Identity=34.3478260869565, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI20129315, Length=230, Percent_Identity=34.3478260869565, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI18859875, Length=418, Percent_Identity=25.8373205741627, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 42362; Mature: 42231

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEVKVPVLPESVSDATIASWHKKAGEIVKRDENIVDLETDKVVLEVPSPVDGVLKEIK
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCHHHHHHHHH
FDAGSTVTSNQVLAIIEEGSIVTAPSPAPSQVIDQKPVAVSAPAAKSNVDSLPPGARFTA
CCCCCEEECCCEEEEEECCCEEECCCCCCHHHHCCCCCEEECCCCCCCCCCCCCCCEEEE
TTEGIDPAQIEGSGRRGAVTKEDIINFAKQNGAARASGTRLEERIPMTRIRQRIAERLMQ
ECCCCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHH
SKNSTAMLTTFNEINLAKVSNIRKELQEEFQKAHGIKLGFMSFFVKAAANALQRFPLVNA
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
SIDGTDIIYHGYSDISIAVSTDKGLVTPVLRNVERMSFADIEHRIADYAKKARDGKLSLE
CCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ELQGGTFTVTNGGTFGSLLSTPIVNPPQSAILGMHTIKERPIAENGNIVIAPMMYVALSY
HCCCCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHCC
DHRIIDGKDSVQFLVDIKNQLEDPGRMLFGL
CCEEECCCCCHHHHEEHHHHCCCCCHHHCCC
>Mature Secondary Structure 
STEVKVPVLPESVSDATIASWHKKAGEIVKRDENIVDLETDKVVLEVPSPVDGVLKEIK
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCEEEEECCCCHHHHHHHHH
FDAGSTVTSNQVLAIIEEGSIVTAPSPAPSQVIDQKPVAVSAPAAKSNVDSLPPGARFTA
CCCCCEEECCCEEEEEECCCEEECCCCCCHHHHCCCCCEEECCCCCCCCCCCCCCCEEEE
TTEGIDPAQIEGSGRRGAVTKEDIINFAKQNGAARASGTRLEERIPMTRIRQRIAERLMQ
ECCCCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHH
SKNSTAMLTTFNEINLAKVSNIRKELQEEFQKAHGIKLGFMSFFVKAAANALQRFPLVNA
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
SIDGTDIIYHGYSDISIAVSTDKGLVTPVLRNVERMSFADIEHRIADYAKKARDGKLSLE
CCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
ELQGGTFTVTNGGTFGSLLSTPIVNPPQSAILGMHTIKERPIAENGNIVIAPMMYVALSY
HCCCCEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHCC
DHRIIDGKDSVQFLVDIKNQLEDPGRMLFGL
CCEEECCCCCHHHHEEHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]