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The map label for this gene is trpA

Identifier: 182681201

GI number: 182681201

Start: 762059

End: 762865

Strand: Direct

Name: trpA

Synonym: XfasM23_0645

Alternate gene names: 182681201

Gene position: 762059-762865 (Clockwise)

Preceding gene: 182681200

Following gene: 182681203

Centisome position: 30.05

GC content: 58.49

Gene sequence:

>807_bases
ATGCATCGTATAGACGAAACATTCCGCCGTTTAAGGGCGCAATCGCGCAAGGCGTTGATCCCTTTCATCACTGCTGGCGA
CCCCTCTTTGGAGGCGGCTGTTCCGGTGATGCATGCTTTGGTCCGTGCGGGTGCGGATGTGATTGAGTTGGGTGTGCCGT
TTTCGGACCCGATGGCGGATGGTCCGGTGATTCAGCACAGTTCCGAGCGCGCGTTGCAGCGTGGTGTGGGTTTGGCCTAT
GTGTTGCAGACAGTCGATGTGTTCCGTCAGAGTGATGCAGTGACGCCGGTGGTCTTGATGGGCTACCTCAATCCTTTGGA
AATCTATGGCATTGCGCGTTTTACACAGCAGGCACTGGCTTCTGGAGTGGATGGGGTGCTGCTTGTGGATCTTCCTCCGG
AAGAGGCGGACGAGATCCGCGCGATCTTTTCGGCGGCTGGTTTGGCACTCATCGTTTTGGCATCTCCGACGACCTCTGCC
TCCCGTCTGGCCACGTTGAGTGGGGTTGCCCAGGGGTACTTGTATTACGTGAGTTTTGCTGGCGTGACTGGTGCTGATCG
GCTTGATGCGCAGTCAGCCGGTGATCGCTTGCGTGGATTACGTGCGCAGACTCAAGTCCCGGTGGTGGTTGGCTTTGGTA
TCAGGGATGCGGCCAGTGCTGTGGTGATGGCGGTGGATGCGGATGGGGTGGTGGTTGGCAGTGCCTTGGTGACGGCCTTA
AGTGATGCTCCGGATGTGGACACCGCCTGCCGGCGTGCTGATGCGTTTTTGGCACCGTTGCGTCAGGCGTTGGATGCGGT
GAAGTAG

Upstream 100 bases:

>100_bases
AGGGTGCGGTGATAGCACTGTGGCCATTGTCTGGCGGAGACCAGGTGATGTTGCCGTGATGCCAATGATTTTCTGAATTG
CAAAATTTTCCGAGATTTCA

Downstream 100 bases:

>100_bases
GTGGTTGGTTTCAATCTGGGTGCGGTTGAATTTGATTGTCGGGCTGGGCTTGGTTGGGGATGTGCCGAGTTTTCTCTGAC
TGTGTGTATGGGGAGGGGAG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MHRIDETFRRLRAQSRKALIPFITAGDPSLEAAVPVMHALVRAGADVIELGVPFSDPMADGPVIQHSSERALQRGVGLAY
VLQTVDVFRQSDAVTPVVLMGYLNPLEIYGIARFTQQALASGVDGVLLVDLPPEEADEIRAIFSAAGLALIVLASPTTSA
SRLATLSGVAQGYLYYVSFAGVTGADRLDAQSAGDRLRGLRAQTQVPVVVGFGIRDAASAVVMAVDADGVVVGSALVTAL
SDAPDVDTACRRADAFLAPLRQALDAVK

Sequences:

>Translated_268_residues
MHRIDETFRRLRAQSRKALIPFITAGDPSLEAAVPVMHALVRAGADVIELGVPFSDPMADGPVIQHSSERALQRGVGLAY
VLQTVDVFRQSDAVTPVVLMGYLNPLEIYGIARFTQQALASGVDGVLLVDLPPEEADEIRAIFSAAGLALIVLASPTTSA
SRLATLSGVAQGYLYYVSFAGVTGADRLDAQSAGDRLRGLRAQTQVPVVVGFGIRDAASAVVMAVDADGVVVGSALVTAL
SDAPDVDTACRRADAFLAPLRQALDAVK
>Mature_268_residues
MHRIDETFRRLRAQSRKALIPFITAGDPSLEAAVPVMHALVRAGADVIELGVPFSDPMADGPVIQHSSERALQRGVGLAY
VLQTVDVFRQSDAVTPVVLMGYLNPLEIYGIARFTQQALASGVDGVLLVDLPPEEADEIRAIFSAAGLALIVLASPTTSA
SRLATLSGVAQGYLYYVSFAGVTGADRLDAQSAGDRLRGLRAQTQVPVVVGFGIRDAASAVVMAVDADGVVVGSALVTAL
SDAPDVDTACRRADAFLAPLRQALDAVK

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=214, Percent_Identity=35.981308411215, Blast_Score=141, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6321412, Length=244, Percent_Identity=41.3934426229508, Blast_Score=193, Evalue=2e-50,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_XYLF2 (B2I9J3)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829361.1
- ProteinModelPortal:   B2I9J3
- SMR:   B2I9J3
- GeneID:   6203301
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0645
- HOGENOM:   HBG391690
- OMA:   VILMTYY
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28079; Mature: 28079

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRIDETFRRLRAQSRKALIPFITAGDPSLEAAVPVMHALVRAGADVIELGVPFSDPMAD
CCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQHSSERALQRGVGLAYVLQTVDVFRQSDAVTPVVLMGYLNPLEIYGIARFTQQALA
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
SGVDGVLLVDLPPEEADEIRAIFSAAGLALIVLASPTTSASRLATLSGVAQGYLYYVSFA
CCCCCEEEEECCCCHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHEEEEEEC
GVTGADRLDAQSAGDRLRGLRAQTQVPVVVGFGIRDAASAVVMAVDADGVVVGSALVTAL
CCCCCCHHCHHHHHHHHHCCHHCCCCCEEEECCCHHHCCEEEEEECCCCEEHHHHHHHHH
SDAPDVDTACRRADAFLAPLRQALDAVK
CCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHRIDETFRRLRAQSRKALIPFITAGDPSLEAAVPVMHALVRAGADVIELGVPFSDPMAD
CCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQHSSERALQRGVGLAYVLQTVDVFRQSDAVTPVVLMGYLNPLEIYGIARFTQQALA
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
SGVDGVLLVDLPPEEADEIRAIFSAAGLALIVLASPTTSASRLATLSGVAQGYLYYVSFA
CCCCCEEEEECCCCHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHEEEEEEC
GVTGADRLDAQSAGDRLRGLRAQTQVPVVVGFGIRDAASAVVMAVDADGVVVGSALVTAL
CCCCCCHHCHHHHHHHHHCCHHCCCCCEEEECCCHHHCCEEEEEECCCCEEHHHHHHHHH
SDAPDVDTACRRADAFLAPLRQALDAVK
CCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA