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The map label for this gene is rpoD [H]

Identifier: 182681181

GI number: 182681181

Start: 734508

End: 736379

Strand: Direct

Name: rpoD [H]

Synonym: XfasM23_0623

Alternate gene names: 182681181

Gene position: 734508-736379 (Clockwise)

Preceding gene: 182681179

Following gene: 182681183

Centisome position: 28.97

GC content: 47.06

Gene sequence:

>1872_bases
ATGCGAAGCCCCATCATGTCCAATGAACGAACTGCTCAGCAATCCGATATCAAGCAACTGATCAGCAAGGGGTTGGAGCA
GGGTTACCTGACTTACGCCGAAGTCAATGATCACCTACCAGAGGATCTGGTGGATCCTGATCAAATTGAAGACATTATTG
GGATGATTAACGGAATGGGCATTGAGGTCCATGAAGTGGCTCCCGATGCTGAGACTTTGTTGCTTAACGAAGGAAACACT
GGGAACAGGGAAGTCGACGATACCGCTGCAGAAGAGGCCGCAGCTGCGCTTAGCGCCCTTGATACCGAAGCAGGACGTAC
TACCGATCCGGTACGTATGTACATGCGTGAGATGGGTACGGTCGAACTATTAACCCGTGAAGGTGAAATAGCAATTGCAA
AGCGTATTGAAGAAGGCTTGAGCGAGGTACAAGCTGCTTTAGGCGCTTTCCCGTTATCTACGGAATTACTGCTTAGTGAA
TATGAGTTGCATAAAGAAGGTAAGCGGCGTCTGACTGAGGTTGTCGTTGGCTTTAATGATTTGATAGAGGAGGATAACTC
CGTAACCTCCTCAGTGGACGTTACTGACGAGTCCATTGTTGAGGTTGACGAAGAAGACGATGACAGTGTCGGTAATGATG
AGGATGCGCCTATGATTGGTCCTGATCCTGCCGAAGTTGCTTCGCGTATCGAATCTCTGTCCACTGAGTATGCCAAGTTC
AAGAAACTTTACGCCAAGCATGGCCCTGAGCACAAAGCAGTCATTAAGGTGCGTGAAGATGTGGCTGCGATCTTTGTGAC
GTTCAAACTGTCGTTGCCATTGACCGATTTGCTTGTTCAGCAGCTACAGAATATGGTGAATTCTGTCAAGGATCATGAGC
GCAAAGTGCTTCACTTGGCGACAAATGTAGCCAAAATGCCGCGTAAAGACTTCATCCGTTCCTGGGAAAACAACCAGACA
AATTTAGAATGGGTGGAGGATGCGATTAAGCGCAAGCAGAAATGGTCCTCTGCGTTGCGTGATATCAAAGATCAGATCAC
CGCTGAGCAGCAAGCTTCCATTGATTTGGAGAAAGTCAATTCTTTGACGTTAGCCGACATCAAAGAGATTAACCGTATGA
TGGTTTATGGTGAAGCAAAAGCATCCAAGGCGAAGAAAGAGATGGTTGAAGCTAATCTTCGCTTAGTGATATCGATTGCT
AAGAAGTATACCAACCGAGGGTTGCAGTTCTTGGATTTGATACAGGAAGGCAACATCGGTTTGATGAAAGCAGTGTGTAA
GTTTGAACACCGTCGTGGCTTTAAGTTCTCCACCTACGCGACTTGGTGGATCCGTCAGGCAATTACTCGTTCTATTGCCG
ATCAGGCGCGCACCATTCGTATCCCGGTGCATATGATTGAGACGATCAACAAACTTAACCGTATCTCTCGTCAAATGTTG
CAGCAGTTTGGTCGCGAGGCGACTCCAGAGGAATTGGCTAAGGAAATGGACATGCCTGAGGACAAGATCCGTAAGGTAAT
GAAGATTGCTAAAGAGCCAATCTCAATGGAGACTCCGATCGGAGATGATGAAGATTCTCATCTGGGTGACTTCATTGAGG
ATCCTAATGTTGAGTCTCCTGTGGATACCACAACCAATGTCAACTTGTCGGAAACAGTGCGTGAAGTGTTGGCTGGTTTG
ACACCAAGAGAGGCGAAGGTTCTGCGTATGCGTTTCGGTATTGACATGAATACAGACCACACTCTGGAGGAGGTGGGTAA
ACAATTTGATGTCACTCGTGAACGTATTCGCCAGATTGAAGCCAAAGCATTACGGAAACTTCGTCATCCAAGCCGTTCTG
AACAACTGCGCTCATTCCTTGATATTGACTGA

Upstream 100 bases:

>100_bases
CTCAATTAGGTGTTTCAGGGCCGCTAAAAATCCTCTGCTGTTAAGTACGAGAAACGGTATTGAACTGTTATAATACTTTT
ACTTGTATAAGTCTTTGAGA

Downstream 100 bases:

>100_bases
ATCAGAGCTGCTCATGGATCTTTCCCATACGTGCTTAATCACGTGTAGGGCAAGCAAATGTGATCTGGTGCGATGTTGAT
GCGGCATTGCCTTTGTTCGT

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 623; Mature: 623

Protein sequence:

>623_residues
MRSPIMSNERTAQQSDIKQLISKGLEQGYLTYAEVNDHLPEDLVDPDQIEDIIGMINGMGIEVHEVAPDAETLLLNEGNT
GNREVDDTAAEEAAAALSALDTEAGRTTDPVRMYMREMGTVELLTREGEIAIAKRIEEGLSEVQAALGAFPLSTELLLSE
YELHKEGKRRLTEVVVGFNDLIEEDNSVTSSVDVTDESIVEVDEEDDDSVGNDEDAPMIGPDPAEVASRIESLSTEYAKF
KKLYAKHGPEHKAVIKVREDVAAIFVTFKLSLPLTDLLVQQLQNMVNSVKDHERKVLHLATNVAKMPRKDFIRSWENNQT
NLEWVEDAIKRKQKWSSALRDIKDQITAEQQASIDLEKVNSLTLADIKEINRMMVYGEAKASKAKKEMVEANLRLVISIA
KKYTNRGLQFLDLIQEGNIGLMKAVCKFEHRRGFKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQML
QQFGREATPEELAKEMDMPEDKIRKVMKIAKEPISMETPIGDDEDSHLGDFIEDPNVESPVDTTTNVNLSETVREVLAGL
TPREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEQLRSFLDID

Sequences:

>Translated_623_residues
MRSPIMSNERTAQQSDIKQLISKGLEQGYLTYAEVNDHLPEDLVDPDQIEDIIGMINGMGIEVHEVAPDAETLLLNEGNT
GNREVDDTAAEEAAAALSALDTEAGRTTDPVRMYMREMGTVELLTREGEIAIAKRIEEGLSEVQAALGAFPLSTELLLSE
YELHKEGKRRLTEVVVGFNDLIEEDNSVTSSVDVTDESIVEVDEEDDDSVGNDEDAPMIGPDPAEVASRIESLSTEYAKF
KKLYAKHGPEHKAVIKVREDVAAIFVTFKLSLPLTDLLVQQLQNMVNSVKDHERKVLHLATNVAKMPRKDFIRSWENNQT
NLEWVEDAIKRKQKWSSALRDIKDQITAEQQASIDLEKVNSLTLADIKEINRMMVYGEAKASKAKKEMVEANLRLVISIA
KKYTNRGLQFLDLIQEGNIGLMKAVCKFEHRRGFKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQML
QQFGREATPEELAKEMDMPEDKIRKVMKIAKEPISMETPIGDDEDSHLGDFIEDPNVESPVDTTTNVNLSETVREVLAGL
TPREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEQLRSFLDID
>Mature_623_residues
MRSPIMSNERTAQQSDIKQLISKGLEQGYLTYAEVNDHLPEDLVDPDQIEDIIGMINGMGIEVHEVAPDAETLLLNEGNT
GNREVDDTAAEEAAAALSALDTEAGRTTDPVRMYMREMGTVELLTREGEIAIAKRIEEGLSEVQAALGAFPLSTELLLSE
YELHKEGKRRLTEVVVGFNDLIEEDNSVTSSVDVTDESIVEVDEEDDDSVGNDEDAPMIGPDPAEVASRIESLSTEYAKF
KKLYAKHGPEHKAVIKVREDVAAIFVTFKLSLPLTDLLVQQLQNMVNSVKDHERKVLHLATNVAKMPRKDFIRSWENNQT
NLEWVEDAIKRKQKWSSALRDIKDQITAEQQASIDLEKVNSLTLADIKEINRMMVYGEAKASKAKKEMVEANLRLVISIA
KKYTNRGLQFLDLIQEGNIGLMKAVCKFEHRRGFKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQML
QQFGREATPEELAKEMDMPEDKIRKVMKIAKEPISMETPIGDDEDSHLGDFIEDPNVESPVDTTTNVNLSETVREVLAGL
TPREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEQLRSFLDID

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=612, Percent_Identity=58.4967320261438, Blast_Score=672, Evalue=0.0,
Organism=Escherichia coli, GI1789098, Length=233, Percent_Identity=41.6309012875536, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI1789871, Length=241, Percent_Identity=28.6307053941909, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=27.7227722772277, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 70523; Mature: 70523

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSPIMSNERTAQQSDIKQLISKGLEQGYLTYAEVNDHLPEDLVDPDQIEDIIGMINGMG
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEHHHCCCCCCCCCHHHHHHHHHHHHCCC
IEVHEVAPDAETLLLNEGNTGNREVDDTAAEEAAAALSALDTEAGRTTDPVRMYMREMGT
EEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
VELLTREGEIAIAKRIEEGLSEVQAALGAFPLSTELLLSEYELHKEGKRRLTEVVVGFND
CEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIEEDNSVTSSVDVTDESIVEVDEEDDDSVGNDEDAPMIGPDPAEVASRIESLSTEYAKF
HHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KKLYAKHGPEHKAVIKVREDVAAIFVTFKLSLPLTDLLVQQLQNMVNSVKDHERKVLHLA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNVAKMPRKDFIRSWENNQTNLEWVEDAIKRKQKWSSALRDIKDQITAEQQASIDLEKVN
HHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHC
SLTLADIKEINRMMVYGEAKASKAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIG
CHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHH
LMKAVCKFEHRRGFKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQML
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHH
QQFGREATPEELAKEMDMPEDKIRKVMKIAKEPISMETPIGDDEDSHLGDFIEDPNVESP
HHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCC
VDTTTNVNLSETVREVLAGLTPREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIE
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AKALRKLRHPSRSEQLRSFLDID
HHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MRSPIMSNERTAQQSDIKQLISKGLEQGYLTYAEVNDHLPEDLVDPDQIEDIIGMINGMG
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEHHHCCCCCCCCCHHHHHHHHHHHHCCC
IEVHEVAPDAETLLLNEGNTGNREVDDTAAEEAAAALSALDTEAGRTTDPVRMYMREMGT
EEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
VELLTREGEIAIAKRIEEGLSEVQAALGAFPLSTELLLSEYELHKEGKRRLTEVVVGFND
CEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIEEDNSVTSSVDVTDESIVEVDEEDDDSVGNDEDAPMIGPDPAEVASRIESLSTEYAKF
HHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KKLYAKHGPEHKAVIKVREDVAAIFVTFKLSLPLTDLLVQQLQNMVNSVKDHERKVLHLA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNVAKMPRKDFIRSWENNQTNLEWVEDAIKRKQKWSSALRDIKDQITAEQQASIDLEKVN
HHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHC
SLTLADIKEINRMMVYGEAKASKAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIG
CHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHH
LMKAVCKFEHRRGFKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQML
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHH
QQFGREATPEELAKEMDMPEDKIRKVMKIAKEPISMETPIGDDEDSHLGDFIEDPNVESP
HHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCC
VDTTTNVNLSETVREVLAGLTPREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIE
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AKALRKLRHPSRSEQLRSFLDID
HHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]