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The map label for this gene is alkR [H]

Identifier: 182681101

GI number: 182681101

Start: 646378

End: 647235

Strand: Direct

Name: alkR [H]

Synonym: XfasM23_0543

Alternate gene names: 182681101

Gene position: 646378-647235 (Clockwise)

Preceding gene: 182681099

Following gene: 182681103

Centisome position: 25.49

GC content: 55.01

Gene sequence:

>858_bases
ATGAGTATCGAAGTGACGGGTGCATGTACTGATCGTCTGGAGGCGTTGCTGAATCACTTTCCTGTTCGCGCGCGGATGTT
CCATTCGGGTACCTTGTGCGGTATCACGGATTTTTCCGTTCCGGCTGAAGGCGGTCAGGTACATCTGCTCAGAAGTGGGG
CGATGGATGTTATTCATCCAGGTAGGGAGTTGCTTCAGGTGAGTGTTCCCAGTTTGTTGTTCTATCCGCGCCCCATGTCC
CGTCGCTTTGTTGCCGACGCGCAATGTGGCGCTGACTTGGTTTGTGCGCAACTTGACTTCGAAGGTGGGAGTGCGAATCA
AGTGGCGGGGGCGTTACCAGATGTCGTCTGTTTACCCTTGGAGAACATTGATGGCGCCGCTCAGGTGCTGGAGCTGCTGT
TTGATGAAGCGTTTGGCGATCATTGCGGTCGCCGTGTGTTGATTGATCGGTTGTTCGAGGTGGTGTTGATTCAGATGTTG
CGATATCTGATGGAAACGGGCGGGGTGGCCGGTGGCATGTTGGCCGGTTTGTCGCATCCCAAGCTGCGCAAGGTGTTGGT
TGCCATGCACGAGCAGCCGGAACGGGGATGGTCATTGGAGGCGTTGGCCTATCTTGCGGGGATGTCTCGCAGTGTGTTCG
CTGGTACTTTTCGTCGTGTTGTCGGTTGTACCCCTGGTGTCTATCTGCAAGGTTGGCGCGTCAAGCTGGCTCAGCAAGCT
TTACGTCAAGGACGACAGTTGTTAAAGACGATTGCTGTTGAGGTTGGTTATGGCAGTGAAGCTGCATTGTCCCGTGCATT
CAAAGCGCAATGTGGTATGACTCCCCGTGAGTGGCGGCAGAGGCAGGATGAGGACTGA

Upstream 100 bases:

>100_bases
TCCTTTGTGATGTAAGGGGTTTGGTTGGATTCCTTTGGTAAGATGAATTTTATGACTCAATTAAGTATTTTTAGTCACTA
AAAGTACTGAAATATTGATA

Downstream 100 bases:

>100_bases
AGTCAGTAAGCGTGATAGTAGTTTCTGCGCGCAGGGTGTCGCCAGGGATGCACTATTCAGTTGAGATAACTTGGATAATG
ATGAATTGATTTTTTTTGTC

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSIEVTGACTDRLEALLNHFPVRARMFHSGTLCGITDFSVPAEGGQVHLLRSGAMDVIHPGRELLQVSVPSLLFYPRPMS
RRFVADAQCGADLVCAQLDFEGGSANQVAGALPDVVCLPLENIDGAAQVLELLFDEAFGDHCGRRVLIDRLFEVVLIQML
RYLMETGGVAGGMLAGLSHPKLRKVLVAMHEQPERGWSLEALAYLAGMSRSVFAGTFRRVVGCTPGVYLQGWRVKLAQQA
LRQGRQLLKTIAVEVGYGSEAALSRAFKAQCGMTPREWRQRQDED

Sequences:

>Translated_285_residues
MSIEVTGACTDRLEALLNHFPVRARMFHSGTLCGITDFSVPAEGGQVHLLRSGAMDVIHPGRELLQVSVPSLLFYPRPMS
RRFVADAQCGADLVCAQLDFEGGSANQVAGALPDVVCLPLENIDGAAQVLELLFDEAFGDHCGRRVLIDRLFEVVLIQML
RYLMETGGVAGGMLAGLSHPKLRKVLVAMHEQPERGWSLEALAYLAGMSRSVFAGTFRRVVGCTPGVYLQGWRVKLAQQA
LRQGRQLLKTIAVEVGYGSEAALSRAFKAQCGMTPREWRQRQDED
>Mature_284_residues
SIEVTGACTDRLEALLNHFPVRARMFHSGTLCGITDFSVPAEGGQVHLLRSGAMDVIHPGRELLQVSVPSLLFYPRPMSR
RFVADAQCGADLVCAQLDFEGGSANQVAGALPDVVCLPLENIDGAAQVLELLFDEAFGDHCGRRVLIDRLFEVVLIQMLR
YLMETGGVAGGMLAGLSHPKLRKVLVAMHEQPERGWSLEALAYLAGMSRSVFAGTFRRVVGCTPGVYLQGWRVKLAQQAL
RQGRQLLKTIAVEVGYGSEAALSRAFKAQCGMTPREWRQRQDED

Specific function: This protein activates the expression of the alkane 1- monooxygenase AlkM [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786496, Length=255, Percent_Identity=27.0588235294118, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 31183; Mature: 31051

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEVTGACTDRLEALLNHFPVRARMFHSGTLCGITDFSVPAEGGQVHLLRSGAMDVIHP
CCEEECCCHHHHHHHHHHHCCHHHHHCCCCCEECCCCCCCCCCCCEEEEEECCCHHHHCC
GRELLQVSVPSLLFYPRPMSRRFVADAQCGADLVCAQLDFEGGSANQVAGALPDVVCLPL
CHHHHHHHCCHHEECCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCHHHHCCCCEEEEEC
ENIDGAAQVLELLFDEAFGDHCGRRVLIDRLFEVVLIQMLRYLMETGGVAGGMLAGLSHP
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCH
KLRKVLVAMHEQPERGWSLEALAYLAGMSRSVFAGTFRRVVGCTPGVYLQGWRVKLAQQA
HHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEECHHHHHHHHHH
LRQGRQLLKTIAVEVGYGSEAALSRAFKAQCGMTPREWRQRQDED
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCC
>Mature Secondary Structure 
SIEVTGACTDRLEALLNHFPVRARMFHSGTLCGITDFSVPAEGGQVHLLRSGAMDVIHP
CEEECCCHHHHHHHHHHHCCHHHHHCCCCCEECCCCCCCCCCCCEEEEEECCCHHHHCC
GRELLQVSVPSLLFYPRPMSRRFVADAQCGADLVCAQLDFEGGSANQVAGALPDVVCLPL
CHHHHHHHCCHHEECCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCHHHHCCCCEEEEEC
ENIDGAAQVLELLFDEAFGDHCGRRVLIDRLFEVVLIQMLRYLMETGGVAGGMLAGLSHP
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCH
KLRKVLVAMHEQPERGWSLEALAYLAGMSRSVFAGTFRRVVGCTPGVYLQGWRVKLAQQA
HHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEECHHHHHHHHHH
LRQGRQLLKTIAVEVGYGSEAALSRAFKAQCGMTPREWRQRQDED
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9546151 [H]