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The map label for this gene is cls [H]

Identifier: 182681044

GI number: 182681044

Start: 584490

End: 585893

Strand: Direct

Name: cls [H]

Synonym: XfasM23_0483

Alternate gene names: 182681044

Gene position: 584490-585893 (Clockwise)

Preceding gene: 182681043

Following gene: 182681045

Centisome position: 23.05

GC content: 53.63

Gene sequence:

>1404_bases
ATGGAAGGGACTTGGCTGTTTGCATTGGATTGGGTGATCCGTTTGGTTGCGTTGCTTTGGATCCCAGCACGAACCACCCC
TAGTGCTGCGCGCAGCTGGTTGTTACTGATTGGTTTCGTCCCTGTGTTTGGCTTGCCACTTTACTTGGTGTTTGGCCATC
CTTGGCTGTCACGTAAGCGTGTGTTGCGTCAGGCGTTGGCTTCTCATGTGATCCGTGAGTGGCAGCGTGGGTTGGGTGCA
TTGCGCTGGCAGCCTCAGGAGAATGGCGTCGCTGCGGAATTGGTACCTTTGGTGGAGCATCAGGGCGATTTTATGCCAAC
ACGTGGTAACGCCTTGGATCTATTGGATGATTACCAAGCTTCGCTGCATTTGTTGATCAATGATATTGATTCCGCGTGCG
AACGTGTGCACTTGCTGTATTACTTGATGTTCGATGATGCGGTGGGTGATGCGGTTGTGGCTGCATTACAGTGTGCTGCG
GCGCGTGGCGTTTCTTGCCGATTGCTGCTGGATGCAGTCGGTGCTAAACGCGGTCTACGTGCCTATCAGCAGTGCCTTCG
TGCCCATGGTGTGGAGGTGCACGCTATGTTGCCTGGTGGGTTGCGCTGGCGTCGCAGTGGGCGTATGGATTTGCGCAACC
ACCGTAAGATTGCTGTGATCGACAATCGTGTCGCTTATATCGGTTCGCAGAATTTGGCATGCCCAGATTTTGTGCCGCAT
TTCCCCAACCGTGAATTGGTCGCACGCGTGTGCGGCCCTGCGGTAAGTCATCTGGAGGCGGTGTTCCTCAGTGATTGGTT
TGTTGAAACTGGTCAACGTTTGGGGATGCGTCCAGATGTTCCCATATGTGAGCAAAATGTTGCGGCCCAGCTAGTGGCCA
GCGGTCCGGCTTACCCGTACGAGAATGCGCGTGATGCGGTGAATACAATGATCCATTTGGCACGGCGGCGTATTGTCTTG
ACGACGCCGTACTTCGTGCCTGATGAGGCCACATTCAGTGCGTTGCGTATTGCAGGCACGTCTGGAGTAGACGTGCAGTT
GATCCTGTCCGAGAGTAATAACCAGTTTCTGATGGCCTGGGCTCAGGAGGCGTACTTTGAGGAGCTGTTGCGCGCAGGAA
TCAAAATTGCGTTGTATCGTCCCCATTTCCTGCATGCCAAGCATCTGAGTGTGGATGATGATATTGCGTTGTTCGGATCG
ATTAATTTGGACATCCGCTCGTTCGCGCTTAACGCTGAGATTGGCATGATTTGTTACGACTCACGTATTGTGACTCGGTT
ACGTGAGGTGGAGAGTGATTATCTGGCCAATGCGCGCCAGCTTAATTTGGTTGAGTGGCAGCAACGTCCCGCTTGGCGTC
GTAGCCGTGAGGGTATTGCACGCTTGGCAGATGCTCTGATGTAG

Upstream 100 bases:

>100_bases
GTGGAGTCAGGCGCTAGTTGGTTGCTTTGCGATGACTCCGAAAGGTTCTGACATAGGGCCGTCCTGACTTTGTGCTGAGG
AAAGTTCTTTGTAGGTTTGG

Downstream 100 bases:

>100_bases
GTGTTGTGGATGCGTGTCGCGTGCCGCTATTGCAATCTTGAGTTATCCGATTGCTGATGTTTGCGCTGTGGTCGTTGTTG
GTGAGGTTGCAGGTGCGATG

Product: phospholipase D/transphosphatidylase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MEGTWLFALDWVIRLVALLWIPARTTPSAARSWLLLIGFVPVFGLPLYLVFGHPWLSRKRVLRQALASHVIREWQRGLGA
LRWQPQENGVAAELVPLVEHQGDFMPTRGNALDLLDDYQASLHLLINDIDSACERVHLLYYLMFDDAVGDAVVAALQCAA
ARGVSCRLLLDAVGAKRGLRAYQQCLRAHGVEVHAMLPGGLRWRRSGRMDLRNHRKIAVIDNRVAYIGSQNLACPDFVPH
FPNRELVARVCGPAVSHLEAVFLSDWFVETGQRLGMRPDVPICEQNVAAQLVASGPAYPYENARDAVNTMIHLARRRIVL
TTPYFVPDEATFSALRIAGTSGVDVQLILSESNNQFLMAWAQEAYFEELLRAGIKIALYRPHFLHAKHLSVDDDIALFGS
INLDIRSFALNAEIGMICYDSRIVTRLREVESDYLANARQLNLVEWQQRPAWRRSREGIARLADALM

Sequences:

>Translated_467_residues
MEGTWLFALDWVIRLVALLWIPARTTPSAARSWLLLIGFVPVFGLPLYLVFGHPWLSRKRVLRQALASHVIREWQRGLGA
LRWQPQENGVAAELVPLVEHQGDFMPTRGNALDLLDDYQASLHLLINDIDSACERVHLLYYLMFDDAVGDAVVAALQCAA
ARGVSCRLLLDAVGAKRGLRAYQQCLRAHGVEVHAMLPGGLRWRRSGRMDLRNHRKIAVIDNRVAYIGSQNLACPDFVPH
FPNRELVARVCGPAVSHLEAVFLSDWFVETGQRLGMRPDVPICEQNVAAQLVASGPAYPYENARDAVNTMIHLARRRIVL
TTPYFVPDEATFSALRIAGTSGVDVQLILSESNNQFLMAWAQEAYFEELLRAGIKIALYRPHFLHAKHLSVDDDIALFGS
INLDIRSFALNAEIGMICYDSRIVTRLREVESDYLANARQLNLVEWQQRPAWRRSREGIARLADALM
>Mature_467_residues
MEGTWLFALDWVIRLVALLWIPARTTPSAARSWLLLIGFVPVFGLPLYLVFGHPWLSRKRVLRQALASHVIREWQRGLGA
LRWQPQENGVAAELVPLVEHQGDFMPTRGNALDLLDDYQASLHLLINDIDSACERVHLLYYLMFDDAVGDAVVAALQCAA
ARGVSCRLLLDAVGAKRGLRAYQQCLRAHGVEVHAMLPGGLRWRRSGRMDLRNHRKIAVIDNRVAYIGSQNLACPDFVPH
FPNRELVARVCGPAVSHLEAVFLSDWFVETGQRLGMRPDVPICEQNVAAQLVASGPAYPYENARDAVNTMIHLARRRIVL
TTPYFVPDEATFSALRIAGTSGVDVQLILSESNNQFLMAWAQEAYFEELLRAGIKIALYRPHFLHAKHLSVDDDIALFGS
INLDIRSFALNAEIGMICYDSRIVTRLREVESDYLANARQLNLVEWQQRPAWRRSREGIARLADALM

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=467, Percent_Identity=34.0471092077088, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI1787007, Length=359, Percent_Identity=27.2980501392758, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 52570; Mature: 52570

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGTWLFALDWVIRLVALLWIPARTTPSAARSWLLLIGFVPVFGLPLYLVFGHPWLSRKR
CCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHCCCCHHHHH
VLRQALASHVIREWQRGLGALRWQPQENGVAAELVPLVEHQGDFMPTRGNALDLLDDYQA
HHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
SLHLLINDIDSACERVHLLYYLMFDDAVGDAVVAALQCAAARGVSCRLLLDAVGAKRGLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCHHHHHH
AYQQCLRAHGVEVHAMLPGGLRWRRSGRMDLRNHRKIAVIDNRVAYIGSQNLACPDFVPH
HHHHHHHHCCCEEEEECCCCCEECCCCCCCCCCCCEEEEEECEEEEECCCCCCCCCCCCC
FPNRELVARVCGPAVSHLEAVFLSDWFVETGQRLGMRPDVPICEQNVAAQLVASGPAYPY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCC
ENARDAVNTMIHLARRRIVLTTPYFVPDEATFSALRIAGTSGVDVQLILSESNNQFLMAW
CCHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHEEEECCCCCEEEEEEECCCCEEEEEH
AQEAYFEELLRAGIKIALYRPHFLHAKHLSVDDDIALFGSINLDIRSFALNAEIGMICYD
HHHHHHHHHHHCCCEEEEECCCEECEECCCCCCCEEEEEEECEEEEEEEECCCEEEEEEC
SRIVTRLREVESDYLANARQLNLVEWQQRPAWRRSREGIARLADALM
HHHHHHHHHHHHHHHCCCHHCCHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEGTWLFALDWVIRLVALLWIPARTTPSAARSWLLLIGFVPVFGLPLYLVFGHPWLSRKR
CCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHCCCCHHHHH
VLRQALASHVIREWQRGLGALRWQPQENGVAAELVPLVEHQGDFMPTRGNALDLLDDYQA
HHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
SLHLLINDIDSACERVHLLYYLMFDDAVGDAVVAALQCAAARGVSCRLLLDAVGAKRGLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCHHHHHH
AYQQCLRAHGVEVHAMLPGGLRWRRSGRMDLRNHRKIAVIDNRVAYIGSQNLACPDFVPH
HHHHHHHHCCCEEEEECCCCCEECCCCCCCCCCCCEEEEEECEEEEECCCCCCCCCCCCC
FPNRELVARVCGPAVSHLEAVFLSDWFVETGQRLGMRPDVPICEQNVAAQLVASGPAYPY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCC
ENARDAVNTMIHLARRRIVLTTPYFVPDEATFSALRIAGTSGVDVQLILSESNNQFLMAW
CCHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHHEEEECCCCCEEEEEEECCCCEEEEEH
AQEAYFEELLRAGIKIALYRPHFLHAKHLSVDDDIALFGSINLDIRSFALNAEIGMICYD
HHHHHHHHHHHCCCEEEEECCCEECEECCCCCCCEEEEEEECEEEEEEEECCCEEEEEEC
SRIVTRLREVESDYLANARQLNLVEWQQRPAWRRSREGIARLADALM
HHHHHHHHHHHHHHHCCCHHCCHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA