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The map label for this gene is rsuA [H]

Identifier: 182681039

GI number: 182681039

Start: 580557

End: 581267

Strand: Direct

Name: rsuA [H]

Synonym: XfasM23_0478

Alternate gene names: 182681039

Gene position: 580557-581267 (Clockwise)

Preceding gene: 182681038

Following gene: 182681040

Centisome position: 22.9

GC content: 52.6

Gene sequence:

>711_bases
ATGAAATTACTCAAGTACATTGCTAATCTTGGTTATGGGAGCCGTAAAGAGGTTGCCCGAATATTTAGAGAAGGCCGTAT
CACGAATGATGAAGGTGAAGTGTTGTATGCCGATGACCATGTACAACACACTGCGGTTCGTTTTGATGGTGAACCGTTGG
ATCCGCCGCCTGGATTAATGGTGATGCTGCATAAGCCTTCTGGTTATATTTGTTCGACCAAGGACGCGGGGCGCTTGGTG
TATGACTTGTTGCCTGCCCGTTTCCGCTTGCGCTCCCCGTTGCTTTCCACGGTCGGTCGGCTTGATGGGGATACCAGTGG
TCTGTTGTTACTCACGGATGATGGTGCACTGCTGCACCGGATGATCTCGCCTAAGACACATCTTCCTAAAGTCTATGAGG
CGACACTCGTGCAAGCGTTGCGGGGAGACGAAGCAACGTTGTTTGCCAGCGGCACTCTCCTGTTGGATGGAGATACAAAG
CCGTTGCAACCAGCAGTGTTGGAAGTACTGGGACCATGCCAGGTACGGTTGACGTTGTATGAGGGGCGTTATCACCAAGT
GCGACGAATGTTTGGTGCAACCGGAAACCGTGTCAACACGTTGCATCGCAGTGCTATCGGTGGCTTGGTCCTGGATGGAC
TTCCGGAAGGGCAGTGGCGTGTGCTTGAAGCCCAGGATCTTGCGATGCTGTTTTCACAGGGGAGCCGATGA

Upstream 100 bases:

>100_bases
GTTTTGGTCAGCCAGCTCATTTTCGTGCTTGCCAATCACCACTTACTATATGGGCAGATGTTGAGAGTGAGTCACGGCAT
GATGCGCAGGGCAGGTCTGT

Downstream 100 bases:

>100_bases
TGGTGAGTGCTCCGTTGTCATTCATGCCCGAGGCGGTGATCTTCGACATGGATGGTCTGATGCTTGATAGCGAGCGCGTG
AGCATCGAATGTCGGTGTCG

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MKLLKYIANLGYGSRKEVARIFREGRITNDEGEVLYADDHVQHTAVRFDGEPLDPPPGLMVMLHKPSGYICSTKDAGRLV
YDLLPARFRLRSPLLSTVGRLDGDTSGLLLLTDDGALLHRMISPKTHLPKVYEATLVQALRGDEATLFASGTLLLDGDTK
PLQPAVLEVLGPCQVRLTLYEGRYHQVRRMFGATGNRVNTLHRSAIGGLVLDGLPEGQWRVLEAQDLAMLFSQGSR

Sequences:

>Translated_236_residues
MKLLKYIANLGYGSRKEVARIFREGRITNDEGEVLYADDHVQHTAVRFDGEPLDPPPGLMVMLHKPSGYICSTKDAGRLV
YDLLPARFRLRSPLLSTVGRLDGDTSGLLLLTDDGALLHRMISPKTHLPKVYEATLVQALRGDEATLFASGTLLLDGDTK
PLQPAVLEVLGPCQVRLTLYEGRYHQVRRMFGATGNRVNTLHRSAIGGLVLDGLPEGQWRVLEAQDLAMLFSQGSR
>Mature_236_residues
MKLLKYIANLGYGSRKEVARIFREGRITNDEGEVLYADDHVQHTAVRFDGEPLDPPPGLMVMLHKPSGYICSTKDAGRLV
YDLLPARFRLRSPLLSTVGRLDGDTSGLLLLTDDGALLHRMISPKTHLPKVYEATLVQALRGDEATLFASGTLLLDGDTK
PLQPAVLEVLGPCQVRLTLYEGRYHQVRRMFGATGNRVNTLHRSAIGGLVLDGLPEGQWRVLEAQDLAMLFSQGSR

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=237, Percent_Identity=43.0379746835443, Blast_Score=152, Evalue=1e-38,
Organism=Escherichia coli, GI1787524, Length=242, Percent_Identity=33.8842975206612, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1790453, Length=236, Percent_Identity=27.9661016949153, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87081838, Length=182, Percent_Identity=31.8681318681319, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26060; Mature: 26060

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLKYIANLGYGSRKEVARIFREGRITNDEGEVLYADDHVQHTAVRFDGEPLDPPPGLM
CHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCEE
VMLHKPSGYICSTKDAGRLVYDLLPARFRLRSPLLSTVGRLDGDTSGLLLLTDDGALLHR
EEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHH
MISPKTHLPKVYEATLVQALRGDEATLFASGTLLLDGDTKPLQPAVLEVLGPCQVRLTLY
HHCCCCCCCHHHHHHHHHHHCCCCEEEEECCEEEECCCCCCCCHHHHHHCCCCEEEEEEE
EGRYHQVRRMFGATGNRVNTLHRSAIGGLVLDGLPEGQWRVLEAQDLAMLFSQGSR
CCHHHHHHHHHCCCCCHHHHHHHHHHCCCEECCCCCCCEEEECHHHHHHHHCCCCC
>Mature Secondary Structure
MKLLKYIANLGYGSRKEVARIFREGRITNDEGEVLYADDHVQHTAVRFDGEPLDPPPGLM
CHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCEE
VMLHKPSGYICSTKDAGRLVYDLLPARFRLRSPLLSTVGRLDGDTSGLLLLTDDGALLHR
EEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHH
MISPKTHLPKVYEATLVQALRGDEATLFASGTLLLDGDTKPLQPAVLEVLGPCQVRLTLY
HHCCCCCCCHHHHHHHHHHHCCCCEEEEECCEEEECCCCCCCCHHHHHHCCCCEEEEEEE
EGRYHQVRRMFGATGNRVNTLHRSAIGGLVLDGLPEGQWRVLEAQDLAMLFSQGSR
CCHHHHHHHHHCCCCCHHHHHHHHHHCCCEECCCCCCCEEEECHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]