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The map label for this gene is rpoA

Identifier: 182681018

GI number: 182681018

Start: 548959

End: 549957

Strand: Direct

Name: rpoA

Synonym: XfasM23_0457

Alternate gene names: 182681018

Gene position: 548959-549957 (Clockwise)

Preceding gene: 182681017

Following gene: 182681019

Centisome position: 21.65

GC content: 43.94

Gene sequence:

>999_bases
ATGACGGTTACCGTCAGCCAGGTTCTGCGTCCTCGAGGTCCGCAGATCGAACGTCTTACAGAAAACCGTGCCAAGGTTGT
ACTTGAGCCTTTGGGTCGCGGGTATGCTCATACATTAGGTAATGCATTGCGTCGTGTCCTGCTTTCTTCGATTCCGGGAT
TCGCGATTACAGAAGTGGAAATTGATGGTGTGTTGCATGAGTACACAACAGTCGAAGGTTTGCAGGAAGATGTGCTTGAA
GTTTTGTTAAATCTTAAGGATGTGGCGATCCGTATACATAGTGGTGATACTGCTACTCTTTCTTTATTTAAACAAGGAGC
AGGAGTGGTGACTGCTGCGGATATTAAGACGGACCATAATGTTGAAATTATCAATGATGGTCACGTCATATGTCATTTGA
CTAAAGACACTACAATTAATATGCGTTTGAAAATTGAGCGTGGTTTTGGTTATCAGCCTGCTGTTGTCCGTCGTCGTCCT
GATGATGAAAATAGAACCATTGGTCGTTTGATTTTGGATGCCTCGTTTTCTCCGGTTCGTCGTGTTGCTTATGTCGTTGA
GGCGGCACGTGTAGAGCAACGTACTGATTTGGATAAACTCATTATTGACATCGAAACAAATGGTACGATAGACGCTGAAG
AAGCTCTTCGTACTGCTGCTGATATTCTTACTGATCAGCTGTCGGTGTTTGGTGATTTTACGCACCGTGATCGTGGTACA
GTCAAACCTGCTAGTAGTGGTGTGGATCCTGTGCTGCTTCGTCCAATTGATGATTTGGAGTTGACGGTGCGTTCTGCTAA
TTGTTTGAAGGCGGAGAGTATTTATTATATTGGTGATCTTATCCAAAAAACAGAGGTTGAGTTGCTTAAGACTCCCAATT
TAGGTAAGAAGTCGCTTACTGAGATTAAAGAAGTTCTTGGCCAACGTGGTTTAGGACTTGGAGTGAAGTTGGAGAATTGG
CCGCCTCCAGGTGTTTCTCAATATGGAATGCTTGGTTAA

Upstream 100 bases:

>100_bases
TTCGGATATCAATGAAGCTTTGATTGTAGAGTTGTATTCAAAATAATTTATATTGGAAATCCTCCATTTTTTAAAGGAGG
TTTATAGGAGATCACGCGAC

Downstream 100 bases:

>100_bases
ACTTGGATTTGGGGGCAGATGAAGAATCTGTGTCTCGCCATCTCTTAGAAACTAATTTATTAGTCCATGGACTAACGAGA
TGATGGTGATAATCCCTATG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTVTVSQVLRPRGPQIERLTENRAKVVLEPLGRGYAHTLGNALRRVLLSSIPGFAITEVEIDGVLHEYTTVEGLQEDVLE
VLLNLKDVAIRIHSGDTATLSLFKQGAGVVTAADIKTDHNVEIINDGHVICHLTKDTTINMRLKIERGFGYQPAVVRRRP
DDENRTIGRLILDASFSPVRRVAYVVEAARVEQRTDLDKLIIDIETNGTIDAEEALRTAADILTDQLSVFGDFTHRDRGT
VKPASSGVDPVLLRPIDDLELTVRSANCLKAESIYYIGDLIQKTEVELLKTPNLGKKSLTEIKEVLGQRGLGLGVKLENW
PPPGVSQYGMLG

Sequences:

>Translated_332_residues
MTVTVSQVLRPRGPQIERLTENRAKVVLEPLGRGYAHTLGNALRRVLLSSIPGFAITEVEIDGVLHEYTTVEGLQEDVLE
VLLNLKDVAIRIHSGDTATLSLFKQGAGVVTAADIKTDHNVEIINDGHVICHLTKDTTINMRLKIERGFGYQPAVVRRRP
DDENRTIGRLILDASFSPVRRVAYVVEAARVEQRTDLDKLIIDIETNGTIDAEEALRTAADILTDQLSVFGDFTHRDRGT
VKPASSGVDPVLLRPIDDLELTVRSANCLKAESIYYIGDLIQKTEVELLKTPNLGKKSLTEIKEVLGQRGLGLGVKLENW
PPPGVSQYGMLG
>Mature_331_residues
TVTVSQVLRPRGPQIERLTENRAKVVLEPLGRGYAHTLGNALRRVLLSSIPGFAITEVEIDGVLHEYTTVEGLQEDVLEV
LLNLKDVAIRIHSGDTATLSLFKQGAGVVTAADIKTDHNVEIINDGHVICHLTKDTTINMRLKIERGFGYQPAVVRRRPD
DENRTIGRLILDASFSPVRRVAYVVEAARVEQRTDLDKLIIDIETNGTIDAEEALRTAADILTDQLSVFGDFTHRDRGTV
KPASSGVDPVLLRPIDDLELTVRSANCLKAESIYYIGDLIQKTEVELLKTPNLGKKSLTEIKEVLGQRGLGLGVKLENWP
PPGVSQYGMLG

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=327, Percent_Identity=62.691131498471, Blast_Score=416, Evalue=1e-117,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_XYLF2 (B2I8J3)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829178.1
- ProteinModelPortal:   B2I8J3
- GeneID:   6203320
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0457
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 36554; Mature: 36423

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTVSQVLRPRGPQIERLTENRAKVVLEPLGRGYAHTLGNALRRVLLSSIPGFAITEVE
CEEEHHHHHCCCCCCHHHHHHHHHHEEHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
IDGVLHEYTTVEGLQEDVLEVLLNLKDVAIRIHSGDTATLSLFKQGAGVVTAADIKTDHN
ECCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHCCCCEEEEEECCCCCC
VEIINDGHVICHLTKDTTINMRLKIERGFGYQPAVVRRRPDDENRTIGRLILDASFSPVR
EEEEECCEEEEEEECCCEEEEEEEEECCCCCCCHHEECCCCCCCCEEEEEEEECCCCHHH
RVAYVVEAARVEQRTDLDKLIIDIETNGTIDAEEALRTAADILTDQLSVFGDFTHRDRGT
HHHHHHHHHHHHHHCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VKPASSGVDPVLLRPIDDLELTVRSANCLKAESIYYIGDLIQKTEVELLKTPNLGKKSLT
CCCCCCCCCCEEECCCCCCEEEEECCCCEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHH
EIKEVLGQRGLGLGVKLENWPPPGVSQYGMLG
HHHHHHHCCCCCCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVTVSQVLRPRGPQIERLTENRAKVVLEPLGRGYAHTLGNALRRVLLSSIPGFAITEVE
EEEHHHHHCCCCCCHHHHHHHHHHEEHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
IDGVLHEYTTVEGLQEDVLEVLLNLKDVAIRIHSGDTATLSLFKQGAGVVTAADIKTDHN
ECCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHCCCCEEEEEECCCCCC
VEIINDGHVICHLTKDTTINMRLKIERGFGYQPAVVRRRPDDENRTIGRLILDASFSPVR
EEEEECCEEEEEEECCCEEEEEEEEECCCCCCCHHEECCCCCCCCEEEEEEEECCCCHHH
RVAYVVEAARVEQRTDLDKLIIDIETNGTIDAEEALRTAADILTDQLSVFGDFTHRDRGT
HHHHHHHHHHHHHHCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VKPASSGVDPVLLRPIDDLELTVRSANCLKAESIYYIGDLIQKTEVELLKTPNLGKKSLT
CCCCCCCCCCEEECCCCCCEEEEECCCCEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHH
EIKEVLGQRGLGLGVKLENWPPPGVSQYGMLG
HHHHHHHCCCCCCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA