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The map label for this gene is pmbA [H]

Identifier: 182680976

GI number: 182680976

Start: 518697

End: 520064

Strand: Direct

Name: pmbA [H]

Synonym: XfasM23_0414

Alternate gene names: 182680976

Gene position: 518697-520064 (Clockwise)

Preceding gene: 182680974

Following gene: 182680978

Centisome position: 20.46

GC content: 53.73

Gene sequence:

>1368_bases
TTGAACGTATGTTCCACAGATCTCAATAGTGTTGATGATAGCCAGGTGCGTTTGGATGTTCTGGCTGATATTGCGCAGCG
TTTGCTGGAGCGTGCTCATGCATGTGGTGCTACTCAGGCTGAAGTGGGTTGCAGTGAGGAGCGTGGGTTGGATGTGAATG
TGCGGATGGGCGATGTGGAGACGGTTGAGTCTACGTGTGATCGCAGTATTGGGGTGACTCTGTATTTTGGTCAGCGTAAG
GGCAGTGCTAGTACTGTCGATTTGCAAGAGGCAAGTTTGGAGGCCACAGTTGCACAAGCGTGTGCGATTGCTCGTTATAC
GGAGGATGATCCAGCGAATGGGTTGGCTGATCCAGTGCGAATGGCAACGGTGTTCCCTGATCTGGATTGTTGGCATCCGT
GGACTCTGGAGGCCGGTACTGCGGTGGATTTGGCTCTGGGGTGTGAAGCAGCAGGCCGCGCTGCTGATGCGCGTATTGTC
AATTCTGATGGTGCTTCGGTCAGTACTCGTTTTGCGTTGTCTGTGTATGCCAATTCGCATGGTTTTGTCGGTCGTGAGCG
TATGACTCAGCATTCGATGAGTTGTGCGTTGATCGCTGGTGATGGGGATGGAATGCAGCGTGATGGTTGGTATACCAACG
CTCTGGCACGAGAGGATTTGGAGTTGCCTGTTGCAGTTGGACGTAAGGCTGCGGAGCGCACTGTTGCGCGGTTGCAGCCG
CGTTCTTTGGCGACGACTCAGTTACCAGTATTGTTCGTTCCAGAAGTTGCACGTTCGTTGATTGGGCATTTGCTCAATGC
GGTTTCCGGTGGGGCGTTGTATCGTCGTGCTAGTTTTCTGCTTGATAGTGTTGGGACCCGGTTGTTCCCAGAGTGGTTTG
CGATTAATGAGCGCCCCCATTTGCGCCGTGGGCTTCGCTCGGCGACGTTCGATGCTGAAGGTGTGGCGACTTGTGATGCT
CCGCTGGTTGCAGGCGGGGTATTGCAGCGTTATGTGTTGAGTAGTTATTCGGCACGCAAGCTTGGCTTGCAGAGTACTGC
CAACGCAGGTGGTGTGCACAATCTTGAGGTGATAGCCAATGCTGCTGATCTGGATGCGCTTGTTGCTGGGTTACCGCGTG
GTTTGCTGGTGACTGAATTAATGGGCAGTGGTGTGAATCCGGTGACTGGTGATTATTCGCGTGGGGCGGCTGGTTTTTGG
ATTGAGAATGGGGCGATTGCTTATCCAGTGGATGGTCTGACTATTGCTGGCAATCTGCGTGAGATGTTTTCCGCCATTGA
GGCGGTGGGTGCGGATGTGGATAGGCGTTCGCATGTCAGCATGGGGTCGTTGTTGATCGGGAGGATGATGGTGGCTGGTA
ACGATTGA

Upstream 100 bases:

>100_bases
GTTTCTTTATTACATCCGCGCATTGCACCATTCTTCCACATTTAAAAATAAACTTTGTCGACCGTGTACATAGGCACGCT
GACTTCAACAGGATAAGACA

Downstream 100 bases:

>100_bases
TGCGATGTTGATGTACGTCGTTGATAGGGTTATGGGGAATTTTGTTATTTTTTGTTATGAGGGTTAGTCAAATGCCACTA
TACATCTCAGGCTTCCAGAT

Product: peptidase U62 modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MNVCSTDLNSVDDSQVRLDVLADIAQRLLERAHACGATQAEVGCSEERGLDVNVRMGDVETVESTCDRSIGVTLYFGQRK
GSASTVDLQEASLEATVAQACAIARYTEDDPANGLADPVRMATVFPDLDCWHPWTLEAGTAVDLALGCEAAGRAADARIV
NSDGASVSTRFALSVYANSHGFVGRERMTQHSMSCALIAGDGDGMQRDGWYTNALAREDLELPVAVGRKAAERTVARLQP
RSLATTQLPVLFVPEVARSLIGHLLNAVSGGALYRRASFLLDSVGTRLFPEWFAINERPHLRRGLRSATFDAEGVATCDA
PLVAGGVLQRYVLSSYSARKLGLQSTANAGGVHNLEVIANAADLDALVAGLPRGLLVTELMGSGVNPVTGDYSRGAAGFW
IENGAIAYPVDGLTIAGNLREMFSAIEAVGADVDRRSHVSMGSLLIGRMMVAGND

Sequences:

>Translated_455_residues
MNVCSTDLNSVDDSQVRLDVLADIAQRLLERAHACGATQAEVGCSEERGLDVNVRMGDVETVESTCDRSIGVTLYFGQRK
GSASTVDLQEASLEATVAQACAIARYTEDDPANGLADPVRMATVFPDLDCWHPWTLEAGTAVDLALGCEAAGRAADARIV
NSDGASVSTRFALSVYANSHGFVGRERMTQHSMSCALIAGDGDGMQRDGWYTNALAREDLELPVAVGRKAAERTVARLQP
RSLATTQLPVLFVPEVARSLIGHLLNAVSGGALYRRASFLLDSVGTRLFPEWFAINERPHLRRGLRSATFDAEGVATCDA
PLVAGGVLQRYVLSSYSARKLGLQSTANAGGVHNLEVIANAADLDALVAGLPRGLLVTELMGSGVNPVTGDYSRGAAGFW
IENGAIAYPVDGLTIAGNLREMFSAIEAVGADVDRRSHVSMGSLLIGRMMVAGND
>Mature_455_residues
MNVCSTDLNSVDDSQVRLDVLADIAQRLLERAHACGATQAEVGCSEERGLDVNVRMGDVETVESTCDRSIGVTLYFGQRK
GSASTVDLQEASLEATVAQACAIARYTEDDPANGLADPVRMATVFPDLDCWHPWTLEAGTAVDLALGCEAAGRAADARIV
NSDGASVSTRFALSVYANSHGFVGRERMTQHSMSCALIAGDGDGMQRDGWYTNALAREDLELPVAVGRKAAERTVARLQP
RSLATTQLPVLFVPEVARSLIGHLLNAVSGGALYRRASFLLDSVGTRLFPEWFAINERPHLRRGLRSATFDAEGVATCDA
PLVAGGVLQRYVLSSYSARKLGLQSTANAGGVHNLEVIANAADLDALVAGLPRGLLVTELMGSGVNPVTGDYSRGAAGFW
IENGAIAYPVDGLTIAGNLREMFSAIEAVGADVDRRSHVSMGSLLIGRMMVAGND

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=415, Percent_Identity=46.0240963855422, Blast_Score=388, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48170; Mature: 48170

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVCSTDLNSVDDSQVRLDVLADIAQRLLERAHACGATQAEVGCSEERGLDVNVRMGDVE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHCCCEEEEEECCHH
TVESTCDRSIGVTLYFGQRKGSASTVDLQEASLEATVAQACAIARYTEDDPANGLADPVR
HHHHHCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
MATVFPDLDCWHPWTLEAGTAVDLALGCEAAGRAADARIVNSDGASVSTRFALSVYANSH
HHHHCCCCCCCCCCEECCCCEEEEEECCCCCCCCCCCEEECCCCCCEEEEEEEEEEECCC
GFVGRERMTQHSMSCALIAGDGDGMQRDGWYTNALAREDLELPVAVGRKAAERTVARLQP
CCCCHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHCCCCCCHHHCCHHHHHHHHHCCC
RSLATTQLPVLFVPEVARSLIGHLLNAVSGGALYRRASFLLDSVGTRLFPEWFAINERPH
CCCCHHCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCHH
LRRGLRSATFDAEGVATCDAPLVAGGVLQRYVLSSYSARKLGLQSTANAGGVHNLEVIAN
HHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEHEEEEC
AADLDALVAGLPRGLLVTELMGSGVNPVTGDYSRGAAGFWIENGAIAYPVDGLTIAGNLR
HHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCEEEECCCCEEEECCHH
EMFSAIEAVGADVDRRSHVSMGSLLIGRMMVAGND
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNVCSTDLNSVDDSQVRLDVLADIAQRLLERAHACGATQAEVGCSEERGLDVNVRMGDVE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHCCCEEEEEECCHH
TVESTCDRSIGVTLYFGQRKGSASTVDLQEASLEATVAQACAIARYTEDDPANGLADPVR
HHHHHCCCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
MATVFPDLDCWHPWTLEAGTAVDLALGCEAAGRAADARIVNSDGASVSTRFALSVYANSH
HHHHCCCCCCCCCCEECCCCEEEEEECCCCCCCCCCCEEECCCCCCEEEEEEEEEEECCC
GFVGRERMTQHSMSCALIAGDGDGMQRDGWYTNALAREDLELPVAVGRKAAERTVARLQP
CCCCHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHCCCCCCHHHCCHHHHHHHHHCCC
RSLATTQLPVLFVPEVARSLIGHLLNAVSGGALYRRASFLLDSVGTRLFPEWFAINERPH
CCCCHHCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCHH
LRRGLRSATFDAEGVATCDAPLVAGGVLQRYVLSSYSARKLGLQSTANAGGVHNLEVIAN
HHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEHEEEEC
AADLDALVAGLPRGLLVTELMGSGVNPVTGDYSRGAAGFWIENGAIAYPVDGLTIAGNLR
HHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCEEEECCCCEEEECCHH
EMFSAIEAVGADVDRRSHVSMGSLLIGRMMVAGND
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]