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The map label for this gene is argF' [H]

Identifier: 182680847

GI number: 182680847

Start: 366801

End: 367811

Strand: Direct

Name: argF' [H]

Synonym: XfasM23_0284

Alternate gene names: 182680847

Gene position: 366801-367811 (Clockwise)

Preceding gene: 182680846

Following gene: 182680848

Centisome position: 14.47

GC content: 53.21

Gene sequence:

>1011_bases
ATGGCCTTGAAACACTTTTTGAATACCCAGGACTGGACTTGCTCTGAACTTAATGCACTGCTGACCCAAGCACGCGCGTT
CAAGCACAACAAACTGGGGAACGCCCTGAAGGGCAAGTCCATCGCCTTAGTATTTTTCAACCCATCCATGCGTACCCGCA
GCAGCTTTGAACTGGGAGCATTTCAGTTGGGCGGTCACGCCATCGTTTTACAGCCTGGCAAAGATGCCTGGCCGATTGAG
TTCGATACCGGCACCGTGATGGAAGCAGAGACCGAAGAACACATCTGCGAAGTTGCCAGAGTCCTGGGTCACTACGTTGA
CCTCATCGGCGTGCGTGCGTTCCCGAAATTTTTAGACTGGACTTACGACCGCCAGGACATCGTACTCAACAGCTTTGCCA
AATATTCCCCAGTGCCGGTGATCAACATGGAGACAATCACCCATCCCTGCCAGGAACTGGCACATATCATGGCCTTGCAG
GAGCACTTTGGCACCACAGACTTGCGCGGCAAAAAGTACGTATTGACCTGGACCTATCACCCCAAACCCTTGAATACCGC
CGTGGCCAATTCCGCACTGACCATCGCGACTCGCTTGGGCATGGATGTCACCTTACTGTGCCCAACCCCCGATTACGTCC
TTGATGAGCGTTACATTGATTGGGCGCGGCAGAACATTGCAGACACTGGCAGCACCTTTCAAGTGAGCCACGACATCGAC
AACGCCTACCGTGGTGCTGATGTGATCTACGCCAAGAGTTGGGGCGCACTCCCGTTCTTTGGCAACTGGGCCATGGAAAA
ACCGATACGCGACCAATACAGACACTTTATCGTTGACGAAGCCAAAATGGCGTTGACCAACAACGCCGTCTTCTCGCATT
GCTTACCACTGCGCCGTAACGTCAAGGCGACCGATGCAGTGATGGATGGATCTAACTGCATTGCCATCCATGAAGCTGGT
AACCGCCTGCATGTACAAAAGGCCATCATGGCCGCACTTGCTTCCCAATAA

Upstream 100 bases:

>100_bases
TGTTTGCCACAATTTAAGTGATTAAAAATACGACGCATTCTGTGTGTGTGTCCCCTAGCTTTATTTTGCGGCAACACTTC
ATATTGAAAAGGATCGAACG

Downstream 100 bases:

>100_bases
TTCACGCTATATTTTTTTGGAACTTCCCCCATGAGTAATAAAAACATTGTCTTGGCCTTCTCCGGTGGACTCGACACCAG
CTTTTGCATCCCCTACCTGA

Product: N-acetylornithine carbamoyltransferase

Products: NA

Alternate protein names: AOTCase [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MALKHFLNTQDWTCSELNALLTQARAFKHNKLGNALKGKSIALVFFNPSMRTRSSFELGAFQLGGHAIVLQPGKDAWPIE
FDTGTVMEAETEEHICEVARVLGHYVDLIGVRAFPKFLDWTYDRQDIVLNSFAKYSPVPVINMETITHPCQELAHIMALQ
EHFGTTDLRGKKYVLTWTYHPKPLNTAVANSALTIATRLGMDVTLLCPTPDYVLDERYIDWARQNIADTGSTFQVSHDID
NAYRGADVIYAKSWGALPFFGNWAMEKPIRDQYRHFIVDEAKMALTNNAVFSHCLPLRRNVKATDAVMDGSNCIAIHEAG
NRLHVQKAIMAALASQ

Sequences:

>Translated_336_residues
MALKHFLNTQDWTCSELNALLTQARAFKHNKLGNALKGKSIALVFFNPSMRTRSSFELGAFQLGGHAIVLQPGKDAWPIE
FDTGTVMEAETEEHICEVARVLGHYVDLIGVRAFPKFLDWTYDRQDIVLNSFAKYSPVPVINMETITHPCQELAHIMALQ
EHFGTTDLRGKKYVLTWTYHPKPLNTAVANSALTIATRLGMDVTLLCPTPDYVLDERYIDWARQNIADTGSTFQVSHDID
NAYRGADVIYAKSWGALPFFGNWAMEKPIRDQYRHFIVDEAKMALTNNAVFSHCLPLRRNVKATDAVMDGSNCIAIHEAG
NRLHVQKAIMAALASQ
>Mature_335_residues
ALKHFLNTQDWTCSELNALLTQARAFKHNKLGNALKGKSIALVFFNPSMRTRSSFELGAFQLGGHAIVLQPGKDAWPIEF
DTGTVMEAETEEHICEVARVLGHYVDLIGVRAFPKFLDWTYDRQDIVLNSFAKYSPVPVINMETITHPCQELAHIMALQE
HFGTTDLRGKKYVLTWTYHPKPLNTAVANSALTIATRLGMDVTLLCPTPDYVLDERYIDWARQNIADTGSTFQVSHDIDN
AYRGADVIYAKSWGALPFFGNWAMEKPIRDQYRHFIVDEAKMALTNNAVFSHCLPLRRNVKATDAVMDGSNCIAIHEAGN
RLHVQKAIMAALASQ

Specific function: Catalyzes the conversion of N-acetylornithine to N- acetylcitrulline in an alternative arginine biosynthesis pathway. The enzyme has no activity with ornithine [H]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=301, Percent_Identity=26.9102990033223, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1790703, Length=363, Percent_Identity=31.404958677686, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1786469, Length=356, Percent_Identity=30.8988764044944, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI48994908, Length=333, Percent_Identity=29.7297297297297, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.9 [H]

Molecular weight: Translated: 37726; Mature: 37594

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKHFLNTQDWTCSELNALLTQARAFKHNKLGNALKGKSIALVFFNPSMRTRSSFELGA
CCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCE
FQLGGHAIVLQPGKDAWPIEFDTGTVMEAETEEHICEVARVLGHYVDLIGVRAFPKFLDW
EEECCEEEEECCCCCCCCEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TYDRQDIVLNSFAKYSPVPVINMETITHPCQELAHIMALQEHFGTTDLRGKKYVLTWTYH
CCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEC
PKPLNTAVANSALTIATRLGMDVTLLCPTPDYVLDERYIDWARQNIADTGSTFQVSHDID
CCCCCHHHHCCHHEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCC
NAYRGADVIYAKSWGALPFFGNWAMEKPIRDQYRHFIVDEAKMALTNNAVFSHCLPLRRN
CHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
VKATDAVMDGSNCIAIHEAGNRLHVQKAIMAALASQ
CCHHHHEECCCCEEEEECCCCEEHHHHHHHHHHHCC
>Mature Secondary Structure 
ALKHFLNTQDWTCSELNALLTQARAFKHNKLGNALKGKSIALVFFNPSMRTRSSFELGA
CHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCE
FQLGGHAIVLQPGKDAWPIEFDTGTVMEAETEEHICEVARVLGHYVDLIGVRAFPKFLDW
EEECCEEEEECCCCCCCCEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TYDRQDIVLNSFAKYSPVPVINMETITHPCQELAHIMALQEHFGTTDLRGKKYVLTWTYH
CCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEC
PKPLNTAVANSALTIATRLGMDVTLLCPTPDYVLDERYIDWARQNIADTGSTFQVSHDID
CCCCCHHHHCCHHEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCC
NAYRGADVIYAKSWGALPFFGNWAMEKPIRDQYRHFIVDEAKMALTNNAVFSHCLPLRRN
CHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
VKATDAVMDGSNCIAIHEAGNRLHVQKAIMAALASQ
CCHHHHEECCCCEEEEECCCCEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]