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The map label for this gene is pfp [H]

Identifier: 182680780

GI number: 182680780

Start: 282570

End: 283823

Strand: Reverse

Name: pfp [H]

Synonym: XfasM23_0209

Alternate gene names: 182680780

Gene position: 283823-282570 (Counterclockwise)

Preceding gene: 182680783

Following gene: 182680779

Centisome position: 11.19

GC content: 55.42

Gene sequence:

>1254_bases
ATGGCAAAAGAGACGTTGTTATACGCCCAATCCGGCGGCGTTACCGCTGTTATCAACGCAACCGCCGCCGCGGTCATCAC
CGAAGCACGGTCACACCGCATTAAAGTCCTCGCCGCACGCAATGGCATCCTCGGTGCACTGCGTGAAGACCTGATCGATA
CATCCAAAGAATCAATCACGGCAATCCGTGCCTTGACCCATACCCCAGGAGGCGCATTTGGCTCGTGCCGTTTCAAGCTC
AAATCGCTTGAAGCCGATCACGAAAAATACGAACGGTTACTTGAAGTATTCAAAGCACATAACGTGCGCTGGTTCCTCTA
CAACGGTGGTAATGACTCCGCCGATACAGCGCTCAAAGTCTCACAGTTAGCCAAAACATTTGACTATCCACTGCACTGTA
TCGGCGTCCCTAAAACCATCGACAACGATTTGGCGATCACCGACGCCAGCCCCGGCTTCGGTTCAGCCGCAAAGTACACT
GCTGTCTCCATATGCGAAGCCGCGCGGGATGTCGCAGCGATGGCCGAGACCTCAACTAAGGTTTTCATTTACGAAGCAAT
GGGCCGCCATGCCGGTTGGCTCGCTGCGGCTGCTGGCTTGGCCGGCCAAGGGCAGGAAGAAGCCCCCCACATCATCCTGC
TCCCTGAACGCACCTACGACGAAACAGCATTTCTGGCGAAAGTCCAACAGGTAGTCGACCGAGTCGGTTGGTGCGTAGTA
GTCGCCAGCGAAGGTATCCGCAGCAATGATGGGCGCTTCGTTGCCGATGCTGGCGGCACCACTGATGCCTTTGGTCACAC
GCAACTTGGCGGCGTCGCCGCTTACCTCGCCAACCGAGTGAAACAGGCACTCGGACTCAAAGTGCACTGGACGCTGCCAG
ATTACCTACAACGCTCCGCAAGGCACATCGCCTCCAAAACAGACTGGGAACAAGCACAGGCTGTGGGTAAAGCTGCAGTG
CACTACGCACTGGAAGGCATGAATGCAGTCATGCCTGTCATCGTACGCACCTCTGACACCCCATACCGTTGGAAAATCGA
GCCGGCAGCACTCACCAAGATTGCCAATCACGAAAAAACCTTACCAGCAAGCTTCTTACGCCGTGACGGCTTCGGCATCA
GCGAACGTGCACGCACCTACTTTGAACCATTGATTCGTGGCGAAGCACCCATGCCCTACGAACGCAACGGATTACCGCGA
CACGTCCATCTGAAAAATCAAAGCGTACAGAAACGGCTACCTGCCTGGGAATAA

Upstream 100 bases:

>100_bases
CACTTCGACCCTCTGCCTACACCGCACCATGATGCCGCGCAGCTTAACGCATCCAAGCGATGTCCCGGCAATGTCCTCGC
ACTACAAGAGCTCGTCCCAC

Downstream 100 bases:

>100_bases
CACCCCTCACTGGTCACCTACAGTCTGCTTACTGACTTGAAACGTCATTGAAGGCCCAATCACCGAAAATTCCGCTTGCT
TCCAGCAAGCGGTGATAGCG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MAKETLLYAQSGGVTAVINATAAAVITEARSHRIKVLAARNGILGALREDLIDTSKESITAIRALTHTPGGAFGSCRFKL
KSLEADHEKYERLLEVFKAHNVRWFLYNGGNDSADTALKVSQLAKTFDYPLHCIGVPKTIDNDLAITDASPGFGSAAKYT
AVSICEAARDVAAMAETSTKVFIYEAMGRHAGWLAAAAGLAGQGQEEAPHIILLPERTYDETAFLAKVQQVVDRVGWCVV
VASEGIRSNDGRFVADAGGTTDAFGHTQLGGVAAYLANRVKQALGLKVHWTLPDYLQRSARHIASKTDWEQAQAVGKAAV
HYALEGMNAVMPVIVRTSDTPYRWKIEPAALTKIANHEKTLPASFLRRDGFGISERARTYFEPLIRGEAPMPYERNGLPR
HVHLKNQSVQKRLPAWE

Sequences:

>Translated_417_residues
MAKETLLYAQSGGVTAVINATAAAVITEARSHRIKVLAARNGILGALREDLIDTSKESITAIRALTHTPGGAFGSCRFKL
KSLEADHEKYERLLEVFKAHNVRWFLYNGGNDSADTALKVSQLAKTFDYPLHCIGVPKTIDNDLAITDASPGFGSAAKYT
AVSICEAARDVAAMAETSTKVFIYEAMGRHAGWLAAAAGLAGQGQEEAPHIILLPERTYDETAFLAKVQQVVDRVGWCVV
VASEGIRSNDGRFVADAGGTTDAFGHTQLGGVAAYLANRVKQALGLKVHWTLPDYLQRSARHIASKTDWEQAQAVGKAAV
HYALEGMNAVMPVIVRTSDTPYRWKIEPAALTKIANHEKTLPASFLRRDGFGISERARTYFEPLIRGEAPMPYERNGLPR
HVHLKNQSVQKRLPAWE
>Mature_416_residues
AKETLLYAQSGGVTAVINATAAAVITEARSHRIKVLAARNGILGALREDLIDTSKESITAIRALTHTPGGAFGSCRFKLK
SLEADHEKYERLLEVFKAHNVRWFLYNGGNDSADTALKVSQLAKTFDYPLHCIGVPKTIDNDLAITDASPGFGSAAKYTA
VSICEAARDVAAMAETSTKVFIYEAMGRHAGWLAAAAGLAGQGQEEAPHIILLPERTYDETAFLAKVQQVVDRVGWCVVV
ASEGIRSNDGRFVADAGGTTDAFGHTQLGGVAAYLANRVKQALGLKVHWTLPDYLQRSARHIASKTDWEQAQAVGKAAVH
YALEGMNAVMPVIVRTSDTPYRWKIEPAALTKIANHEKTLPASFLRRDGFGISERARTYFEPLIRGEAPMPYERNGLPRH
VHLKNQSVQKRLPAWE

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Escherichia coli, GI1790350, Length=221, Percent_Identity=25.3393665158371, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321679, Length=214, Percent_Identity=30.3738317757009, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 45432; Mature: 45301

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKETLLYAQSGGVTAVINATAAAVITEARSHRIKVLAARNGILGALREDLIDTSKESIT
CCCCEEEEECCCCEEEEEEHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCCHHHHH
AIRALTHTPGGAFGSCRFKLKSLEADHEKYERLLEVFKAHNVRWFLYNGGNDSADTALKV
HHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHH
SQLAKTFDYPLHCIGVPKTIDNDLAITDASPGFGSAAKYTAVSICEAARDVAAMAETSTK
HHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCE
VFIYEAMGRHAGWLAAAAGLAGQGQEEAPHIILLPERTYDETAFLAKVQQVVDRVGWCVV
EEEEECCCCCCHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEE
VASEGIRSNDGRFVADAGGTTDAFGHTQLGGVAAYLANRVKQALGLKVHWTLPDYLQRSA
EECCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHH
RHIASKTDWEQAQAVGKAAVHYALEGMNAVMPVIVRTSDTPYRWKIEPAALTKIANHEKT
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECHHHHHHHHCCCCC
LPASFLRRDGFGISERARTYFEPLIRGEAPMPYERNGLPRHVHLKNQSVQKRLPAWE
CCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHCCCCCC
>Mature Secondary Structure 
AKETLLYAQSGGVTAVINATAAAVITEARSHRIKVLAARNGILGALREDLIDTSKESIT
CCCEEEEECCCCEEEEEEHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCCHHHHH
AIRALTHTPGGAFGSCRFKLKSLEADHEKYERLLEVFKAHNVRWFLYNGGNDSADTALKV
HHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHH
SQLAKTFDYPLHCIGVPKTIDNDLAITDASPGFGSAAKYTAVSICEAARDVAAMAETSTK
HHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCE
VFIYEAMGRHAGWLAAAAGLAGQGQEEAPHIILLPERTYDETAFLAKVQQVVDRVGWCVV
EEEEECCCCCCHHHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEE
VASEGIRSNDGRFVADAGGTTDAFGHTQLGGVAAYLANRVKQALGLKVHWTLPDYLQRSA
EECCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHH
RHIASKTDWEQAQAVGKAAVHYALEGMNAVMPVIVRTSDTPYRWKIEPAALTKIANHEKT
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEECHHHHHHHHCCCCC
LPASFLRRDGFGISERARTYFEPLIRGEAPMPYERNGLPRHVHLKNQSVQKRLPAWE
CCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10913106 [H]