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The map label for this gene is pnp

Identifier: 182680754

GI number: 182680754

Start: 249029

End: 251131

Strand: Direct

Name: pnp

Synonym: XfasM23_0183

Alternate gene names: 182680754

Gene position: 249029-251131 (Clockwise)

Preceding gene: 182680753

Following gene: 182680756

Centisome position: 9.82

GC content: 50.26

Gene sequence:

>2103_bases
GTGGCAAAAATCACCAAAACCTTCCAGTATGGCAAGCATACCGTGACCCTGGAAACCGGCGAAGTCGCCCGTCAGGCGAG
TGGAGCTGTTATCGTCAAGATGGATGACACTGTGCTTCTGGTCACTGTTGTTGCTGCAAAAACTGCGCGAGAGGGGCAGG
ATTTTTTCCCATTGACGGTTGATTATCAGGAGAAGTTCTATGCCGGTGGGCGGATCCCGGGTAGCTTCTTTAAGCGTGAA
GGCCGTGCCACCGAAAAAGAAACGTTGATTTCTCGATTGATTGACCGTCCGATCCGTCCGTTGTTTCCTGAAGATTACAA
GAACGAAGTTCAGATTATCGCTATGGTTATGTCACTCGATCCGGAAATCGACGGTGATATTCCCGCAATGATTGGTGCTT
CAGCTGCATTATCACTGGCTGGTATTCCGTTTAAGGGACCGATTGGTGCTGCTAAGGTTGGTTACAATGATGGTCAATAT
ATCCTCAATCCCACTGTCAGTGAACTGAAAAAATCTCAGTTGGAGCTTGTTGTCGCTGGTACTGCCAATGCCGTGCTGAT
GGTTGAGTCTGAGGCTGCATTGCTTTCCGAAGAAGTAATGTTAGGGGCGGTTATTTTCGGTCATCGCGAAATGCAAAAAG
TGATTCAGGTGATTGATTCACTGACCGCAGAAGCTTGTACGCAGCCGTCTGATTGGGTGCCCCCGGCCAAGAATGATGCT
TTGGTCATTGCGTTGAAGAAAGTAATTGGTGGTCGCTTGTCCGACGCTTTCCATATCAGAGACAAGTTGCAGCGTCGCGA
CGCGATCGCTGCTGTTAAAGATGATGTGATTCAGCAGTTGGCTGGCCGTCTTGAAGTTGAGGGCTGGAACTTGGCGGAAT
TGTTAAAGGAATTCGGTGAACTGGAATACCGTACTATGCGTGATGCACTGTTGGACACTAAAGTCCGCATTGATGGACGC
TCTCTGGAAGCTGTACGCCCGATCACCGTAAAGGTTGGGGTTCTGCCGCGTGTTCATGGTTCCGGGTTATTTACACGTGG
AGAGACTCAAGCGATTGTAGTCACTACTTTGGGTACAGCGCGCGATGGCCAAGTAATTGATGCAGTCACTGGTGAATATA
AGGAAAATTTTTTATTCCATTACAACTTCCCGCCATATTCAGTGGGCGAATGTGGCCGTTTTGGAGTACCGAAGCGCCGT
GAGATCGGTCACGGCCGTCTTGCTAGGCGCGGGGTGTTGGCTGTGATGCCGAGTTTGGATGAATTCCCATACACGATCCG
TGTTGTCTCTGAGATCACCGAATCGAACGGTTCCTCATCTATGGCTTCGGTGTGCGGTTCCTCGCTGGCGCTCATGGATG
CTGGTGTGCCGGTGAAAGCACCAGTGGCTGGCATTGCGATGGGCTTAGTCAAGGAAGGGGAGCGCTTTGTTGTGTTGAGC
GACATTCTCGGTGACGAAGATCACCTTGGCGATATGGATTTCAAGGTTGCTGGTACCTCCGAAGGAGTGTCCGCGTTACA
GATGGACATTAAGATCGAAGGCATAACAGAAGAGATTATGAGGCAGGCTCTACAGCAGGCTAAAGTTGGACGTCTGCATA
TCCTTGGCGAGATGGACAAGGCACTGGCTGCACCGCGTGCCGAGTTGTCCGACTACGCTCCTCGCCTGCTGACGATTAAG
ATTCATCCTGACAAAATCCGCGATGTCATTGGTAAAGGTGGTTCGACCATCCAGGCGATCACTAAGGAGACTGGAACACA
GATCGATATTCAGGACGATGGTACGATCGTTATCGCTTCAGTCAACAATGCTGCAGCCCGGGAGGCTAAGCGTCGTATTG
AGCAAATTACCTCGGATGTTGAGCCGGGTCGGATTTATGAGGGCAAGGTTGCTAAGATTATGGATTTTGGCGCTTTTGTG
ACTATTTTGCCTGGAAAAGATGGTTTAGTGCACGTTTCCCAGATATCCAGCGAGCGTGTGGAGCGTGTTGGCGACAAGCT
GAAGGAAGGCGATGTGGTTCGTGTGAAGGTCTTAGAAGTTGATAAGCAAGGTCGAATCCGTCTTTCGATCAAAGCAGTGG
AGGAAGAGGTTGCCAGCATATGA

Upstream 100 bases:

>100_bases
GCACTCTACTTCAAGGGATTGTAAATCCTCATAACCGCGTTATTTGCAAACACTCAGCATAGTTGATCCGTTTATAAAGC
ATCATCCTAAGGAAACTCTC

Downstream 100 bases:

>100_bases
CTTTCTACCTGATGTCCTCTGGATAGTGCAGAAAATGAATTCATATAAGGTTTATGCATGTATGGCAGCGCATTACTCGT
TGCCATCATCTGCTTACAAC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 700; Mature: 699

Protein sequence:

>700_residues
MAKITKTFQYGKHTVTLETGEVARQASGAVIVKMDDTVLLVTVVAAKTAREGQDFFPLTVDYQEKFYAGGRIPGSFFKRE
GRATEKETLISRLIDRPIRPLFPEDYKNEVQIIAMVMSLDPEIDGDIPAMIGASAALSLAGIPFKGPIGAAKVGYNDGQY
ILNPTVSELKKSQLELVVAGTANAVLMVESEAALLSEEVMLGAVIFGHREMQKVIQVIDSLTAEACTQPSDWVPPAKNDA
LVIALKKVIGGRLSDAFHIRDKLQRRDAIAAVKDDVIQQLAGRLEVEGWNLAELLKEFGELEYRTMRDALLDTKVRIDGR
SLEAVRPITVKVGVLPRVHGSGLFTRGETQAIVVTTLGTARDGQVIDAVTGEYKENFLFHYNFPPYSVGECGRFGVPKRR
EIGHGRLARRGVLAVMPSLDEFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPVKAPVAGIAMGLVKEGERFVVLS
DILGDEDHLGDMDFKVAGTSEGVSALQMDIKIEGITEEIMRQALQQAKVGRLHILGEMDKALAAPRAELSDYAPRLLTIK
IHPDKIRDVIGKGGSTIQAITKETGTQIDIQDDGTIVIASVNNAAAREAKRRIEQITSDVEPGRIYEGKVAKIMDFGAFV
TILPGKDGLVHVSQISSERVERVGDKLKEGDVVRVKVLEVDKQGRIRLSIKAVEEEVASI

Sequences:

>Translated_700_residues
MAKITKTFQYGKHTVTLETGEVARQASGAVIVKMDDTVLLVTVVAAKTAREGQDFFPLTVDYQEKFYAGGRIPGSFFKRE
GRATEKETLISRLIDRPIRPLFPEDYKNEVQIIAMVMSLDPEIDGDIPAMIGASAALSLAGIPFKGPIGAAKVGYNDGQY
ILNPTVSELKKSQLELVVAGTANAVLMVESEAALLSEEVMLGAVIFGHREMQKVIQVIDSLTAEACTQPSDWVPPAKNDA
LVIALKKVIGGRLSDAFHIRDKLQRRDAIAAVKDDVIQQLAGRLEVEGWNLAELLKEFGELEYRTMRDALLDTKVRIDGR
SLEAVRPITVKVGVLPRVHGSGLFTRGETQAIVVTTLGTARDGQVIDAVTGEYKENFLFHYNFPPYSVGECGRFGVPKRR
EIGHGRLARRGVLAVMPSLDEFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPVKAPVAGIAMGLVKEGERFVVLS
DILGDEDHLGDMDFKVAGTSEGVSALQMDIKIEGITEEIMRQALQQAKVGRLHILGEMDKALAAPRAELSDYAPRLLTIK
IHPDKIRDVIGKGGSTIQAITKETGTQIDIQDDGTIVIASVNNAAAREAKRRIEQITSDVEPGRIYEGKVAKIMDFGAFV
TILPGKDGLVHVSQISSERVERVGDKLKEGDVVRVKVLEVDKQGRIRLSIKAVEEEVASI
>Mature_699_residues
AKITKTFQYGKHTVTLETGEVARQASGAVIVKMDDTVLLVTVVAAKTAREGQDFFPLTVDYQEKFYAGGRIPGSFFKREG
RATEKETLISRLIDRPIRPLFPEDYKNEVQIIAMVMSLDPEIDGDIPAMIGASAALSLAGIPFKGPIGAAKVGYNDGQYI
LNPTVSELKKSQLELVVAGTANAVLMVESEAALLSEEVMLGAVIFGHREMQKVIQVIDSLTAEACTQPSDWVPPAKNDAL
VIALKKVIGGRLSDAFHIRDKLQRRDAIAAVKDDVIQQLAGRLEVEGWNLAELLKEFGELEYRTMRDALLDTKVRIDGRS
LEAVRPITVKVGVLPRVHGSGLFTRGETQAIVVTTLGTARDGQVIDAVTGEYKENFLFHYNFPPYSVGECGRFGVPKRRE
IGHGRLARRGVLAVMPSLDEFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPVKAPVAGIAMGLVKEGERFVVLSD
ILGDEDHLGDMDFKVAGTSEGVSALQMDIKIEGITEEIMRQALQQAKVGRLHILGEMDKALAAPRAELSDYAPRLLTIKI
HPDKIRDVIGKGGSTIQAITKETGTQIDIQDDGTIVIASVNNAAAREAKRRIEQITSDVEPGRIYEGKVAKIMDFGAFVT
ILPGKDGLVHVSQISSERVERVGDKLKEGDVVRVKVLEVDKQGRIRLSIKAVEEEVASI

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=716, Percent_Identity=36.8715083798883, Blast_Score=437, Evalue=1e-122,
Organism=Escherichia coli, GI145693187, Length=694, Percent_Identity=64.8414985590778, Blast_Score=898, Evalue=0.0,
Organism=Escherichia coli, GI87082262, Length=82, Percent_Identity=46.3414634146341, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115534063, Length=708, Percent_Identity=33.4745762711864, Blast_Score=350, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17535281, Length=78, Percent_Identity=47.4358974358974, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI281362905, Length=713, Percent_Identity=37.7279102384292, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651641, Length=713, Percent_Identity=37.7279102384292, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651643, Length=713, Percent_Identity=37.7279102384292, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI161079377, Length=653, Percent_Identity=37.3660030627871, Blast_Score=407, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_XYLF2 (B2I730)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828914.1
- ProteinModelPortal:   B2I730
- SMR:   B2I730
- GeneID:   6202555
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0183
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 76020; Mature: 75888

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKITKTFQYGKHTVTLETGEVARQASGAVIVKMDDTVLLVTVVAAKTAREGQDFFPLTV
CCCCCCHHHCCCEEEEEECHHHHHHCCCEEEEEECCCEEEEEHHHHHHHCCCCCCCEEEE
DYQEKFYAGGRIPGSFFKREGRATEKETLISRLIDRPIRPLFPEDYKNEVQIIAMVMSLD
CCCHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEEHHCC
PEIDGDIPAMIGASAALSLAGIPFKGPIGAAKVGYNDGQYILNPTVSELKKSQLELVVAG
CCCCCCCCHHHCCHHHHHCCCCCCCCCCCCEEECCCCCCEEECCCHHHHHHCCEEEEEEC
TANAVLMVESEAALLSEEVMLGAVIFGHREMQKVIQVIDSLTAEACTQPSDWVPPAKNDA
CCCEEEEEECCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
LVIALKKVIGGRLSDAFHIRDKLQRRDAIAAVKDDVIQQLAGRLEVEGWNLAELLKEFGE
EEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHCC
LEYRTMRDALLDTKVRIDGRSLEAVRPITVKVGVLPRVHGSGLFTRGETQAIVVTTLGTA
CHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCEEECCCCCEECCCCCEEEEEEECCC
RDGQVIDAVTGEYKENFLFHYNFPPYSVGECGRFGVPKRREIGHGRLARRGVLAVMPSLD
CCCCEEEHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCEEECCCCC
EFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPVKAPVAGIAMGLVKEGERFVVLS
CCCCHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEE
DILGDEDHLGDMDFKVAGTSEGVSALQMDIKIEGITEEIMRQALQQAKVGRLHILGEMDK
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHCCCCEEEEEEHHHH
ALAAPRAELSDYAPRLLTIKIHPDKIRDVIGKGGSTIQAITKETGTQIDIQDDGTIVIAS
HHCCCHHHHHCCCCEEEEEEECHHHHHHHHCCCCCEEEHHHHCCCCEEEEECCCEEEEEE
VNNAAAREAKRRIEQITSDVEPGRIYEGKVAKIMDFGAFVTILPGKDGLVHVSQISSERV
CCCHHHHHHHHHHHHHHCCCCCCCEECCCHHHHEECCCEEEEECCCCCEEEHHHHHHHHH
ERVGDKLKEGDVVRVKVLEVDKQGRIRLSIKAVEEEVASI
HHHHHHHCCCCEEEEEEEEECCCCCEEEEEEHHHHHHHCC
>Mature Secondary Structure 
AKITKTFQYGKHTVTLETGEVARQASGAVIVKMDDTVLLVTVVAAKTAREGQDFFPLTV
CCCCCHHHCCCEEEEEECHHHHHHCCCEEEEEECCCEEEEEHHHHHHHCCCCCCCEEEE
DYQEKFYAGGRIPGSFFKREGRATEKETLISRLIDRPIRPLFPEDYKNEVQIIAMVMSLD
CCCHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEEHHCC
PEIDGDIPAMIGASAALSLAGIPFKGPIGAAKVGYNDGQYILNPTVSELKKSQLELVVAG
CCCCCCCCHHHCCHHHHHCCCCCCCCCCCCEEECCCCCCEEECCCHHHHHHCCEEEEEEC
TANAVLMVESEAALLSEEVMLGAVIFGHREMQKVIQVIDSLTAEACTQPSDWVPPAKNDA
CCCEEEEEECCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
LVIALKKVIGGRLSDAFHIRDKLQRRDAIAAVKDDVIQQLAGRLEVEGWNLAELLKEFGE
EEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHCC
LEYRTMRDALLDTKVRIDGRSLEAVRPITVKVGVLPRVHGSGLFTRGETQAIVVTTLGTA
CHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCEEECCCCCEECCCCCEEEEEEECCC
RDGQVIDAVTGEYKENFLFHYNFPPYSVGECGRFGVPKRREIGHGRLARRGVLAVMPSLD
CCCCEEEHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCEEECCCCC
EFPYTIRVVSEITESNGSSSMASVCGSSLALMDAGVPVKAPVAGIAMGLVKEGERFVVLS
CCCCHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEE
DILGDEDHLGDMDFKVAGTSEGVSALQMDIKIEGITEEIMRQALQQAKVGRLHILGEMDK
HHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECHHHHHHHHHHHHCCCCEEEEEEHHHH
ALAAPRAELSDYAPRLLTIKIHPDKIRDVIGKGGSTIQAITKETGTQIDIQDDGTIVIAS
HHCCCHHHHHCCCCEEEEEEECHHHHHHHHCCCCCEEEHHHHCCCCEEEEECCCEEEEEE
VNNAAAREAKRRIEQITSDVEPGRIYEGKVAKIMDFGAFVTILPGKDGLVHVSQISSERV
CCCHHHHHHHHHHHHHHCCCCCCCEECCCHHHHEECCCEEEEECCCCCEEEHHHHHHHHH
ERVGDKLKEGDVVRVKVLEVDKQGRIRLSIKAVEEEVASI
HHHHHHHCCCCEEEEEEEEECCCCCEEEEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA