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The map label for this gene is gcvT

Identifier: 182680707

GI number: 182680707

Start: 191439

End: 192545

Strand: Reverse

Name: gcvT

Synonym: XfasM23_0136

Alternate gene names: 182680707

Gene position: 192545-191439 (Counterclockwise)

Preceding gene: 182680708

Following gene: 182680706

Centisome position: 7.59

GC content: 50.95

Gene sequence:

>1107_bases
ATGATCAAAAAGACCATTCTCAATGACACCCATCAAGCACTCGGTGCCAAGATGGTCGACTTCAGCGGCTGGGAAATGCC
CATTCACTACGGCTCACAGATTGATGAGCATCATCACGTCAGGCGTAACGCCGGCATATTCGACGTCAGTCATATGACTG
TCATTGATTTGCACGGGACACAGGTACGCCCCCTCTTGCGTCGACTATTAGCTAATTCGGTAGATAAGCTCAAAGTACCA
GGAAAAGCACTGTATAGCTGCATGTTGAATCCGCAAGGCGGTGTAATCGACGATTTGATCGTCTATTACCTACGTGAAGA
TTACTTTCGTTTTATCGTCAACGCCGCTACACGAGAAAAGGATTTAGCATGGATCAATACACAAGCATCTGCGTTCAACG
TACGTGTTGAAGAGCGTGCAGACTTGGCAATGCTCGCCGTACAGGGGCCTGCCGCGCGCGCGCAGGTCACCAACCTATTG
GCCGAGACTCACCGCGACGCAGTGGAAAAGCTCGGACGTTTCGCCGCACTAGAAGTCGCATCGCACTCGAAAAAACCCCT
CTTTATTTCACGTACTGGATATACCGGTGAGGACGGCTTTGAGATCTTACTTCCACAAGAAGAAACGATAACGTTATGGA
ATGCGCTGTTGAAGACAGGCGTGAAACCAATCGGACTAGGCGCACGCGATACGTTACGTCTAGAGGCAGGGATGAATCTC
TACGGCCAGGATATGGACGAGCAGGTTTCACCCTACGAAGCAGCACTGGGCTGGACTGTCATGCTTGATGAAGGTCGCAA
CTTCATCGGTCGCAACGTGCTGGAACAGCAAAAGACTAACGGCGTATCACGACAGATGATAGGCCTACTCATGGATGAGA
AGGGAGTGCTTCGCCATGGTCAGAAAGTATTGACCGCACAAGGTGAGGGCCACATCCTTTCCGGTACGTTTTCACCAACC
TTGAACAAGGCTATCGGATTCGCGCGCGTGCCAGCCGGAAAACCAAGTGAGGTCCGAGTCAACATACGTGACCGTGAAAT
CCCGGTGCGGGTTGTCAAATTCCCCTTTGTACGTGAAGGCCAGACGCAACCAAACATTTTTGATTGA

Upstream 100 bases:

>100_bases
TGACTGGCGCCCCTAGCATGGCAACTCAGACCTAAATACCGCACAGCCGTACACTCCCCACTATCCAGAGTGTTGCAAGA
AAAATGAGAGCCTGTACTCC

Downstream 100 bases:

>100_bases
ACGAAAATCGATCTACAACAATCGATTTTCCTCATTTTCACCATCACCACTGTGGGCCGCTCATTGCCACTCGTGCTGCT
GGTGCACCAGCAGCCTCTGT

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MIKKTILNDTHQALGAKMVDFSGWEMPIHYGSQIDEHHHVRRNAGIFDVSHMTVIDLHGTQVRPLLRRLLANSVDKLKVP
GKALYSCMLNPQGGVIDDLIVYYLREDYFRFIVNAATREKDLAWINTQASAFNVRVEERADLAMLAVQGPAARAQVTNLL
AETHRDAVEKLGRFAALEVASHSKKPLFISRTGYTGEDGFEILLPQEETITLWNALLKTGVKPIGLGARDTLRLEAGMNL
YGQDMDEQVSPYEAALGWTVMLDEGRNFIGRNVLEQQKTNGVSRQMIGLLMDEKGVLRHGQKVLTAQGEGHILSGTFSPT
LNKAIGFARVPAGKPSEVRVNIRDREIPVRVVKFPFVREGQTQPNIFD

Sequences:

>Translated_368_residues
MIKKTILNDTHQALGAKMVDFSGWEMPIHYGSQIDEHHHVRRNAGIFDVSHMTVIDLHGTQVRPLLRRLLANSVDKLKVP
GKALYSCMLNPQGGVIDDLIVYYLREDYFRFIVNAATREKDLAWINTQASAFNVRVEERADLAMLAVQGPAARAQVTNLL
AETHRDAVEKLGRFAALEVASHSKKPLFISRTGYTGEDGFEILLPQEETITLWNALLKTGVKPIGLGARDTLRLEAGMNL
YGQDMDEQVSPYEAALGWTVMLDEGRNFIGRNVLEQQKTNGVSRQMIGLLMDEKGVLRHGQKVLTAQGEGHILSGTFSPT
LNKAIGFARVPAGKPSEVRVNIRDREIPVRVVKFPFVREGQTQPNIFD
>Mature_368_residues
MIKKTILNDTHQALGAKMVDFSGWEMPIHYGSQIDEHHHVRRNAGIFDVSHMTVIDLHGTQVRPLLRRLLANSVDKLKVP
GKALYSCMLNPQGGVIDDLIVYYLREDYFRFIVNAATREKDLAWINTQASAFNVRVEERADLAMLAVQGPAARAQVTNLL
AETHRDAVEKLGRFAALEVASHSKKPLFISRTGYTGEDGFEILLPQEETITLWNALLKTGVKPIGLGARDTLRLEAGMNL
YGQDMDEQVSPYEAALGWTVMLDEGRNFIGRNVLEQQKTNGVSRQMIGLLMDEKGVLRHGQKVLTAQGEGHILSGTFSPT
LNKAIGFARVPAGKPSEVRVNIRDREIPVRVVKFPFVREGQTQPNIFD

Specific function: The glycine cleavage system catalyzes the degradation of glycine

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family

Homologues:

Organism=Homo sapiens, GI44662838, Length=370, Percent_Identity=35.6756756756757, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI257796258, Length=342, Percent_Identity=36.2573099415205, Blast_Score=185, Evalue=5e-47,
Organism=Homo sapiens, GI257796254, Length=366, Percent_Identity=32.2404371584699, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI257796256, Length=305, Percent_Identity=34.7540983606557, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI24797151, Length=335, Percent_Identity=28.6567164179104, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI197927446, Length=348, Percent_Identity=26.7241379310345, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI21361378, Length=348, Percent_Identity=26.7241379310345, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI194306651, Length=257, Percent_Identity=27.2373540856031, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1789272, Length=367, Percent_Identity=55.5858310626703, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17560118, Length=374, Percent_Identity=32.8877005347594, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71994045, Length=377, Percent_Identity=24.1379310344828, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71994052, Length=377, Percent_Identity=24.1379310344828, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32563613, Length=268, Percent_Identity=24.6268656716418, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320222, Length=379, Percent_Identity=36.6754617414248, Blast_Score=204, Evalue=2e-53,
Organism=Drosophila melanogaster, GI20129441, Length=377, Percent_Identity=35.8090185676393, Blast_Score=191, Evalue=9e-49,
Organism=Drosophila melanogaster, GI28571104, Length=279, Percent_Identity=26.5232974910394, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI20130091, Length=343, Percent_Identity=23.6151603498542, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GCST_XYLF2 (B2I6Q1)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828867.1
- ProteinModelPortal:   B2I6Q1
- SMR:   B2I6Q1
- GeneID:   6203772
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0136
- HOGENOM:   HBG299834
- OMA:   KALYGGM
- ProtClustDB:   PRK00389
- GO:   GO:0005737
- HAMAP:   MF_00259
- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903
- PIRSF:   PIRSF006487
- TIGRFAMs:   TIGR00528

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: =2.1.2.10

Molecular weight: Translated: 41077; Mature: 41077

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKTILNDTHQALGAKMVDFSGWEMPIHYGSQIDEHHHVRRNAGIFDVSHMTVIDLHGT
CCCCHHCCHHHHHHCCEEEECCCCEEEECCCCCCHHHHHHHHCCCEEEECCEEEEEECCC
QVRPLLRRLLANSVDKLKVPGKALYSCMLNPQGGVIDDLIVYYLREDYFRFIVNAATREK
CHHHHHHHHHHCCHHHCCCCHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DLAWINTQASAFNVRVEERADLAMLAVQGPAARAQVTNLLAETHRDAVEKLGRFAALEVA
CEEEEECCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEEHHH
SHSKKPLFISRTGYTGEDGFEILLPQEETITLWNALLKTGVKPIGLGARDTLRLEAGMNL
CCCCCCEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHCCCCEECCCCCCEEEEECCCCC
YGQDMDEQVSPYEAALGWTVMLDEGRNFIGRNVLEQQKTNGVSRQMIGLLMDEKGVLRHG
CCCCCCHHCCCHHHHCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHCC
QKVLTAQGEGHILSGTFSPTLNKAIGFARVPAGKPSEVRVNIRDREIPVRVVKFPFVREG
CEEEEECCCCEEEECCCCHHHHHHCCEEECCCCCCCEEEEEEECCCCCEEEEECCCEECC
QTQPNIFD
CCCCCCCC
>Mature Secondary Structure
MIKKTILNDTHQALGAKMVDFSGWEMPIHYGSQIDEHHHVRRNAGIFDVSHMTVIDLHGT
CCCCHHCCHHHHHHCCEEEECCCCEEEECCCCCCHHHHHHHHCCCEEEECCEEEEEECCC
QVRPLLRRLLANSVDKLKVPGKALYSCMLNPQGGVIDDLIVYYLREDYFRFIVNAATREK
CHHHHHHHHHHCCHHHCCCCHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
DLAWINTQASAFNVRVEERADLAMLAVQGPAARAQVTNLLAETHRDAVEKLGRFAALEVA
CEEEEECCCCEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEEHHH
SHSKKPLFISRTGYTGEDGFEILLPQEETITLWNALLKTGVKPIGLGARDTLRLEAGMNL
CCCCCCEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHCCCCEECCCCCCEEEEECCCCC
YGQDMDEQVSPYEAALGWTVMLDEGRNFIGRNVLEQQKTNGVSRQMIGLLMDEKGVLRHG
CCCCCCHHCCCHHHHCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHCC
QKVLTAQGEGHILSGTFSPTLNKAIGFARVPAGKPSEVRVNIRDREIPVRVVKFPFVREG
CEEEEECCCCEEEECCCCHHHHHHCCEEECCCCCCCEEEEEEECCCCCEEEEECCCEECC
QTQPNIFD
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA