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The map label for this gene is tyrS [H]

Identifier: 182680696

GI number: 182680696

Start: 169209

End: 170465

Strand: Direct

Name: tyrS [H]

Synonym: XfasM23_0125

Alternate gene names: 182680696

Gene position: 169209-170465 (Clockwise)

Preceding gene: 182680693

Following gene: 182680697

Centisome position: 6.67

GC content: 46.46

Gene sequence:

>1257_bases
ATGTCTTTGGTTTCAAATTCTATTGCTCTCATTGAAAGAGGTGCTAATGAGATACTTAAATTCGATGAATTAGAGCGTCG
CTTGAGAAGCGGGCGTCCGCTTCGGATCAAGGCAGGTTTCGATCCGACTTCTCCTGATCTGCATTTGGGGCATACCGTCT
TGCTGAACAAGATGCGGCAATTTCAGGATCTTGGTCACCAAGTTGTTTTTCTGATTGGTGATTTTACGGGGATGATTGGT
GACCCGACTGGTAAGAACGTTACCCGTAAGCCACTCAGTCGTGAGGATGTATTGGCTAATGCCAGTACCTACGAGGATCA
GGTATTTAAGGTTTTGGATCGTACACTTACCGAGGTGAGGTTCAATTCCGAATGGTTTGAGAAGATGAGTGCTGTCGATA
TGATCAAGCTCGCGGCTCAGCACACTGTTGCTCGCATGCTTGAGCGGGATGACTTCGCCAAGCGCTTTGCTTCTCAGCAG
CCGATTGTGATCCATGAATTCCTTTATCCACTGATTCAGGGCTATGACTCTATTGCTTTGCGAGCTGATGTTGAACTTGG
TGGTACAGATCAGAAGTTTAATTTATTGATGGGGCGAGCCTTGCAAGAGCATCATGGTCAGCCACCTCAGATTGTATTAA
CTATGCCGCTGCTTGAGGGGCTGGATGGTGTGGCTAAGATGTCCAAGTCCTTGGGGAATTACATTGGTATTAAAGAGCCG
CCTATCGACATTGTTACAAAAACTATGAAAATTGGTGACGATCTGATGTGGCGTTGGATCGAACTGCTTTCTTTTAAGAT
CAGCGCCGCTGAAGCGGTTGCATTGCGTGAAGCGGTCGCAAAATCGGAACTGAATCCGCGTGAGGTTAAATTACGTCTAG
CTCATGAGCTAGTGAGCCGTTTCTATGATAATGCAGCAGCCGAAAAAGCAATCGCTGGTTGGCAAGCGGTGGTGACAGGG
CAAGGTAACAACAACCTGTTGCCGTTACAGAAGATTAATGTTCCTGCGGATGGGGTGCGCCTCGTTGCATTGTTGACAAA
GTCTGGACTAGCTCCAAGTAACTCTGAGGCAATGCGAAAGCTTAAGGAGCGAGCTGTACGTGTCGATGGTATTGTGGTGG
ATGATGCTCATCTACATTTTGTGCCAGGCTTTGAGGGATTAATTCAAATTGGTAAGCGCAATTTTATTAAAGTCCGATTG
GTAACTTCTTCGGAATCTCAGGAGTTTCCTGAGTCTAATCGTATTGACAAGAGTTAG

Upstream 100 bases:

>100_bases
GTAATTTTAATTCTATTAATGTTCCAGATGAATTGATCTTCTATTAATTTATTATTAATGCAATTAAAATTTCAGACGAA
TTTTACTACTGAATAATATT

Downstream 100 bases:

>100_bases
AAACATCTTTGAATTTATTTTTGATGTGTATTCCTAATTGCAGTAGGCGTTTGAGTGTGTCTTGCAAACTTGCTGGCTTA
CATACGAAATGATTGATACG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MSLVSNSIALIERGANEILKFDELERRLRSGRPLRIKAGFDPTSPDLHLGHTVLLNKMRQFQDLGHQVVFLIGDFTGMIG
DPTGKNVTRKPLSREDVLANASTYEDQVFKVLDRTLTEVRFNSEWFEKMSAVDMIKLAAQHTVARMLERDDFAKRFASQQ
PIVIHEFLYPLIQGYDSIALRADVELGGTDQKFNLLMGRALQEHHGQPPQIVLTMPLLEGLDGVAKMSKSLGNYIGIKEP
PIDIVTKTMKIGDDLMWRWIELLSFKISAAEAVALREAVAKSELNPREVKLRLAHELVSRFYDNAAAEKAIAGWQAVVTG
QGNNNLLPLQKINVPADGVRLVALLTKSGLAPSNSEAMRKLKERAVRVDGIVVDDAHLHFVPGFEGLIQIGKRNFIKVRL
VTSSESQEFPESNRIDKS

Sequences:

>Translated_418_residues
MSLVSNSIALIERGANEILKFDELERRLRSGRPLRIKAGFDPTSPDLHLGHTVLLNKMRQFQDLGHQVVFLIGDFTGMIG
DPTGKNVTRKPLSREDVLANASTYEDQVFKVLDRTLTEVRFNSEWFEKMSAVDMIKLAAQHTVARMLERDDFAKRFASQQ
PIVIHEFLYPLIQGYDSIALRADVELGGTDQKFNLLMGRALQEHHGQPPQIVLTMPLLEGLDGVAKMSKSLGNYIGIKEP
PIDIVTKTMKIGDDLMWRWIELLSFKISAAEAVALREAVAKSELNPREVKLRLAHELVSRFYDNAAAEKAIAGWQAVVTG
QGNNNLLPLQKINVPADGVRLVALLTKSGLAPSNSEAMRKLKERAVRVDGIVVDDAHLHFVPGFEGLIQIGKRNFIKVRL
VTSSESQEFPESNRIDKS
>Mature_417_residues
SLVSNSIALIERGANEILKFDELERRLRSGRPLRIKAGFDPTSPDLHLGHTVLLNKMRQFQDLGHQVVFLIGDFTGMIGD
PTGKNVTRKPLSREDVLANASTYEDQVFKVLDRTLTEVRFNSEWFEKMSAVDMIKLAAQHTVARMLERDDFAKRFASQQP
IVIHEFLYPLIQGYDSIALRADVELGGTDQKFNLLMGRALQEHHGQPPQIVLTMPLLEGLDGVAKMSKSLGNYIGIKEPP
IDIVTKTMKIGDDLMWRWIELLSFKISAAEAVALREAVAKSELNPREVKLRLAHELVSRFYDNAAAEKAIAGWQAVVTGQ
GNNNLLPLQKINVPADGVRLVALLTKSGLAPSNSEAMRKLKERAVRVDGIVVDDAHLHFVPGFEGLIQIGKRNFIKVRLV
TSSESQEFPESNRIDKS

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=274, Percent_Identity=29.5620437956204, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI1787925, Length=414, Percent_Identity=28.2608695652174, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25145802, Length=210, Percent_Identity=29.0476190476191, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325160, Length=383, Percent_Identity=26.6318537859008, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI20130383, Length=409, Percent_Identity=25.1833740831296, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46707; Mature: 46575

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLVSNSIALIERGANEILKFDELERRLRSGRPLRIKAGFDPTSPDLHLGHTVLLNKMRQ
CCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHH
FQDLGHQVVFLIGDFTGMIGDPTGKNVTRKPLSREDVLANASTYEDQVFKVLDRTLTEVR
HHHCCCEEEEEEECHHCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHH
FNSEWFEKMSAVDMIKLAAQHTVARMLERDDFAKRFASQQPIVIHEFLYPLIQGYDSIAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEE
RADVELGGTDQKFNLLMGRALQEHHGQPPQIVLTMPLLEGLDGVAKMSKSLGNYIGIKEP
EEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHCCHHHHHHHHHHHCCCCCCCC
PIDIVTKTMKIGDDLMWRWIELLSFKISAAEAVALREAVAKSELNPREVKLRLAHELVSR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
FYDNAAAEKAIAGWQAVVTGQGNNNLLPLQKINVPADGVRLVALLTKSGLAPSNSEAMRK
HHHHHHHHHHHCCCEEEEEECCCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHH
LKERAVRVDGIVVDDAHLHFVPGFEGLIQIGKRNFIKVRLVTSSESQEFPESNRIDKS
HHHHHHHCCCEEEECCCEEEECCHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLVSNSIALIERGANEILKFDELERRLRSGRPLRIKAGFDPTSPDLHLGHTVLLNKMRQ
CCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHH
FQDLGHQVVFLIGDFTGMIGDPTGKNVTRKPLSREDVLANASTYEDQVFKVLDRTLTEVR
HHHCCCEEEEEEECHHCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHH
FNSEWFEKMSAVDMIKLAAQHTVARMLERDDFAKRFASQQPIVIHEFLYPLIQGYDSIAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEE
RADVELGGTDQKFNLLMGRALQEHHGQPPQIVLTMPLLEGLDGVAKMSKSLGNYIGIKEP
EEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHCCHHHHHHHHHHHCCCCCCCC
PIDIVTKTMKIGDDLMWRWIELLSFKISAAEAVALREAVAKSELNPREVKLRLAHELVSR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
FYDNAAAEKAIAGWQAVVTGQGNNNLLPLQKINVPADGVRLVALLTKSGLAPSNSEAMRK
HHHHHHHHHHHCCCEEEEEECCCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHH
LKERAVRVDGIVVDDAHLHFVPGFEGLIQIGKRNFIKVRLVTSSESQEFPESNRIDKS
HHHHHHHCCCEEEECCCEEEECCHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]