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The map label for this gene is gyrB

Identifier: 182680577

GI number: 182680577

Start: 4634

End: 7078

Strand: Direct

Name: gyrB

Synonym: XfasM23_0005

Alternate gene names: 182680577

Gene position: 4634-7078 (Clockwise)

Preceding gene: 182680576

Following gene: 182680578

Centisome position: 0.18

GC content: 46.67

Gene sequence:

>2445_bases
ATGACCGAAAAGCAGAATACATCGCTGCTCAGTAATGGCGGCATATATGACTCAAGTAAAATCACAGTACTACGTGGACT
GGATGCTGTTCGCAAGCGTCCAGGGATGTATATCGGAGATGTCCATGACGGTACAGGTTTGCACCACATGGTGTTTGAAG
TTGTCGATAACTCGGTGGATGAGGCATTAGCTGGTCATGCCGACAGCATTCTTGTCAAGATCCATATTGATGGGTCTGTC
TCTGTTTCGGATAACGGTCGAGGTATCCCTGTAGATATTCATAAAGAAGAGGGTGTTTCTGCCGCTGAAGTGATCCTGAC
AGTTTTACATGCTGGTGGTAAGTTCGACGATAATAGCTATAAGGTTTCTGGTGGTTTGCATGGGGTAGGAGTTTCAGTGG
TCAACGCCCTTTCTGAGCGATTATGGCTTGATATTTGGCGTGATGGTTACCACTACCAACAAGAATATGTTTTGGGTGAG
CCGCAATATCCTCTTAAGCAGTTAGAGGTGTCAGCGAAACGCGGTACTACATTACGTTTTAAACCGGCAAAAGAGATCTT
CAGTGATGTGGAGTTTCATTACGAAAATCTTGCAAAGCGCCTCCGTGAGTTATCCTTCCTCAATTCCGGCCTTAAAGTTA
GTTTGATTGATGAACGTGGTGAGGGTCGGCGTGACGATTTTCACTATGAAGGTGGTATTCGTAGCTTTGTAGAACATTTA
GCTCAATTGAAGACCCCATTGCATCCGAATGTAATTTCGGTCACAGGGGAGCACAACGGCATTGTTGTGGATGCTGCTTT
ACAGTGGACTGATGCCTACCAAGAAACAATGTATTGTTTTACAAATAACATTCCACAAAAAGATGGCGGTACCCACCTTG
CTGGCTTCCGTGCTGCATTGACTCGTACGTTGGGTAATTACATTGAGCAGAATGGGGTTGCTAGGCAAGCGAAGATCACT
TTTTCTGGTGATGATATGCGTGAAGGTATGATTGCCGTGTTATCGGTAAAAGTCCCTGAACCTAGTTTTTCTTCCCAAAC
CAAGGAAAAGTTGGTGAGCTCCGATGTCAAGCCGGCAGTGGAAGCTACCTTTGGCTTGCGTTTGGAGGAATTCTTACAAG
AGAATCCTAACGAAGCACGTGCAATTGCTGGAAAGATTGTCGATGCTGCTCGTGCTCGCGAGGCTGCTCGCAAAGCCCGC
GATTTAACCCGTCGAAAAGGTGTGCTTGATATCGCCGGTTTGCCAGGTAAGTTGGCAGATTGCCAAGAGAAAGACCCAGC
GATGTCAGAATTGTTTATCGTCGAAGGGGATTCTGCTGGTGGTTCTGCAAAGCAGGGGCGTAACCGAAAAAATCAGGCAG
TATTGCCGCTCAGGGGCAAGATTCTTAACGTTGAACGTGCACGATTCGACCGTATGCTCTCTAGTGCTGAGGTTGGCACA
TTGATCACGGCCCTTGGGACAGGCATCGGTAAGGACGAATATAACCCAGATAAGCTACGTTACCATCGCGTCATCATTAT
GACCGATGCGGATGTGGATGGTTCGCATATCCGTACTTTATTGCTGACGTTTTTTTACAGGCAAATGCCAGAATTGATTG
AACGTGGCCATATCTATATTGGCTTGCCGCCCTTGTACAAGCTAAAGCAGGGTAAGAGTGAGCTGTACTTAAAGGACGAT
ATCGCATTGAATGTTTATTTGGCGAATAGCGCGGTTGAAGGAGCGCAATTACTCCCCGCTGAAGGTGAGCCGCCCATTGA
GGGTCTTGCATTGGAGAAATTGCTGATTACATATATCGCCGCAAAAGATACTATCGTGCGTCATTCGCATCGCTATGATT
CTCGCCTCCTGGAAGCACTGCTTGAATTTACTCCCTTGGATTCGACTTGCTTTCAATCCTACAGTGATAGCGAACATCTA
CAATCGCTGATGGCATTGCTGAACCAGTCTAGCTTGGGTGCTCCGCGTTATATTCTTAAGATGCAGTTTCCTGATGAACA
ACATTCTGCCGCCTTGTTGGCGACCCGCTATCACATGGGTGAGGAATTGACTCAGGTGTTGCAATTATCAGCATTAGAGA
CTGGTGAGTTACGTCCACTCTATGAAGCGGCCAAGTTGCTACATGGCTTAATTCGTAACGGCGCTAAAATTGTTCGTGGC
ACTAAATCGCAATCAATAGAGTGTTTTGGTGATGCGCAAGCTTGGTTGCTTGAAGAGGCGAAGAAAGGTCGCCAAATCCA
GCGCTTTAAGGGGTTGGGTGAAATGAATCCGGAGCAGTTATGGGATACTACGGTGAATCCGGACACTCGCCGTCTTTTGC
AAGTTTGTATTGAAGATGGGATTGCTGCTGACCAAATCTTCAGCACACTGATGGGTGATGTGGTAGAGCCACGCCGTGGC
TTTATTGAGAACAATGCATTAAAAGTGACTCGCTTGGATATTTAA

Upstream 100 bases:

>100_bases
AGAAGTATATGGATAGATACATTTTTAATTTTAGGATGGAAATTAGCGCCTTGATGCGTCTGAATTTTGAAGTGATTGAC
ATGGAGCCAATGAAAAACGC

Downstream 100 bases:

>100_bases
CTTTATTACGGACAGTTGCTGTTGTTCCATCAGAAAACGATGCTATGTCTAATCTGTTTTTATCAGATTCTGTGCCGACT
AACCGTGTCGCGCGTTTGTC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 814; Mature: 813

Protein sequence:

>814_residues
MTEKQNTSLLSNGGIYDSSKITVLRGLDAVRKRPGMYIGDVHDGTGLHHMVFEVVDNSVDEALAGHADSILVKIHIDGSV
SVSDNGRGIPVDIHKEEGVSAAEVILTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSERLWLDIWRDGYHYQQEYVLGE
PQYPLKQLEVSAKRGTTLRFKPAKEIFSDVEFHYENLAKRLRELSFLNSGLKVSLIDERGEGRRDDFHYEGGIRSFVEHL
AQLKTPLHPNVISVTGEHNGIVVDAALQWTDAYQETMYCFTNNIPQKDGGTHLAGFRAALTRTLGNYIEQNGVARQAKIT
FSGDDMREGMIAVLSVKVPEPSFSSQTKEKLVSSDVKPAVEATFGLRLEEFLQENPNEARAIAGKIVDAARAREAARKAR
DLTRRKGVLDIAGLPGKLADCQEKDPAMSELFIVEGDSAGGSAKQGRNRKNQAVLPLRGKILNVERARFDRMLSSAEVGT
LITALGTGIGKDEYNPDKLRYHRVIIMTDADVDGSHIRTLLLTFFYRQMPELIERGHIYIGLPPLYKLKQGKSELYLKDD
IALNVYLANSAVEGAQLLPAEGEPPIEGLALEKLLITYIAAKDTIVRHSHRYDSRLLEALLEFTPLDSTCFQSYSDSEHL
QSLMALLNQSSLGAPRYILKMQFPDEQHSAALLATRYHMGEELTQVLQLSALETGELRPLYEAAKLLHGLIRNGAKIVRG
TKSQSIECFGDAQAWLLEEAKKGRQIQRFKGLGEMNPEQLWDTTVNPDTRRLLQVCIEDGIAADQIFSTLMGDVVEPRRG
FIENNALKVTRLDI

Sequences:

>Translated_814_residues
MTEKQNTSLLSNGGIYDSSKITVLRGLDAVRKRPGMYIGDVHDGTGLHHMVFEVVDNSVDEALAGHADSILVKIHIDGSV
SVSDNGRGIPVDIHKEEGVSAAEVILTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSERLWLDIWRDGYHYQQEYVLGE
PQYPLKQLEVSAKRGTTLRFKPAKEIFSDVEFHYENLAKRLRELSFLNSGLKVSLIDERGEGRRDDFHYEGGIRSFVEHL
AQLKTPLHPNVISVTGEHNGIVVDAALQWTDAYQETMYCFTNNIPQKDGGTHLAGFRAALTRTLGNYIEQNGVARQAKIT
FSGDDMREGMIAVLSVKVPEPSFSSQTKEKLVSSDVKPAVEATFGLRLEEFLQENPNEARAIAGKIVDAARAREAARKAR
DLTRRKGVLDIAGLPGKLADCQEKDPAMSELFIVEGDSAGGSAKQGRNRKNQAVLPLRGKILNVERARFDRMLSSAEVGT
LITALGTGIGKDEYNPDKLRYHRVIIMTDADVDGSHIRTLLLTFFYRQMPELIERGHIYIGLPPLYKLKQGKSELYLKDD
IALNVYLANSAVEGAQLLPAEGEPPIEGLALEKLLITYIAAKDTIVRHSHRYDSRLLEALLEFTPLDSTCFQSYSDSEHL
QSLMALLNQSSLGAPRYILKMQFPDEQHSAALLATRYHMGEELTQVLQLSALETGELRPLYEAAKLLHGLIRNGAKIVRG
TKSQSIECFGDAQAWLLEEAKKGRQIQRFKGLGEMNPEQLWDTTVNPDTRRLLQVCIEDGIAADQIFSTLMGDVVEPRRG
FIENNALKVTRLDI
>Mature_813_residues
TEKQNTSLLSNGGIYDSSKITVLRGLDAVRKRPGMYIGDVHDGTGLHHMVFEVVDNSVDEALAGHADSILVKIHIDGSVS
VSDNGRGIPVDIHKEEGVSAAEVILTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSERLWLDIWRDGYHYQQEYVLGEP
QYPLKQLEVSAKRGTTLRFKPAKEIFSDVEFHYENLAKRLRELSFLNSGLKVSLIDERGEGRRDDFHYEGGIRSFVEHLA
QLKTPLHPNVISVTGEHNGIVVDAALQWTDAYQETMYCFTNNIPQKDGGTHLAGFRAALTRTLGNYIEQNGVARQAKITF
SGDDMREGMIAVLSVKVPEPSFSSQTKEKLVSSDVKPAVEATFGLRLEEFLQENPNEARAIAGKIVDAARAREAARKARD
LTRRKGVLDIAGLPGKLADCQEKDPAMSELFIVEGDSAGGSAKQGRNRKNQAVLPLRGKILNVERARFDRMLSSAEVGTL
ITALGTGIGKDEYNPDKLRYHRVIIMTDADVDGSHIRTLLLTFFYRQMPELIERGHIYIGLPPLYKLKQGKSELYLKDDI
ALNVYLANSAVEGAQLLPAEGEPPIEGLALEKLLITYIAAKDTIVRHSHRYDSRLLEALLEFTPLDSTCFQSYSDSEHLQ
SLMALLNQSSLGAPRYILKMQFPDEQHSAALLATRYHMGEELTQVLQLSALETGELRPLYEAAKLLHGLIRNGAKIVRGT
KSQSIECFGDAQAWLLEEAKKGRQIQRFKGLGEMNPEQLWDTTVNPDTRRLLQVCIEDGIAADQIFSTLMGDVVEPRRGF
IENNALKVTRLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=543, Percent_Identity=25.7826887661142, Blast_Score=137, Evalue=5e-32,
Organism=Homo sapiens, GI19913406, Length=532, Percent_Identity=24.4360902255639, Blast_Score=129, Evalue=2e-29,
Organism=Escherichia coli, GI48994957, Length=802, Percent_Identity=60.4738154613466, Blast_Score=952, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=546, Percent_Identity=40.1098901098901, Blast_Score=365, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17535065, Length=571, Percent_Identity=25.7443082311734, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=523, Percent_Identity=24.282982791587, Blast_Score=105, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI212645657, Length=154, Percent_Identity=35.0649350649351, Blast_Score=80, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=604, Percent_Identity=26.158940397351, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI17136538, Length=540, Percent_Identity=25.3703703703704, Blast_Score=130, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 90262; Mature: 90131

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKQNTSLLSNGGIYDSSKITVLRGLDAVRKRPGMYIGDVHDGTGLHHMVFEVVDNSVD
CCCCCCCCEECCCCCCCCCCEEEECCCHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHH
EALAGHADSILVKIHIDGSVSVSDNGRGIPVDIHKEEGVSAAEVILTVLHAGGKFDDNSY
HHHCCCCCEEEEEEEECCEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCE
KVSGGLHGVGVSVVNALSERLWLDIWRDGYHYQQEYVLGEPQYPLKQLEVSAKRGTTLRF
EECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHEECCCCCCCHHHHHHHHCCCCEEEE
KPAKEIFSDVEFHYENLAKRLRELSFLNSGLKVSLIDERGEGRRDDFHYEGGIRSFVEHL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHH
AQLKTPLHPNVISVTGEHNGIVVDAALQWTDAYQETMYCFTNNIPQKDGGTHLAGFRAAL
HHHCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
TRTLGNYIEQNGVARQAKITFSGDDMREGMIAVLSVKVPEPSFSSQTKEKLVSSDVKPAV
HHHHHHHHHHCCCCEEEEEEECCCCHHCCEEEEEEEECCCCCCCHHHHHHHHHCCCCHHH
EATFGLRLEEFLQENPNEARAIAGKIVDAARAREAARKARDLTRRKGVLDIAGLPGKLAD
HHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCC
CQEKDPAMSELFIVEGDSAGGSAKQGRNRKNQAVLPLRGKILNVERARFDRMLSSAEVGT
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHH
LITALGTGIGKDEYNPDKLRYHRVIIMTDADVDGSHIRTLLLTFFYRQMPELIERGHIYI
HHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
GLPPLYKLKQGKSELYLKDDIALNVYLANSAVEGAQLLPAEGEPPIEGLALEKLLITYIA
CCCCHHHHHCCCCCEEEECCEEEEEEEECCCCCCCEECCCCCCCCCHHHHHHHHHHHHHH
AKDTIVRHSHRYDSRLLEALLEFTPLDSTCFQSYSDSEHLQSLMALLNQSSLGAPRYILK
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEE
MQFPDEQHSAALLATRYHMGEELTQVLQLSALETGELRPLYEAAKLLHGLIRNGAKIVRG
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHEEEC
TKSQSIECFGDAQAWLLEEAKKGRQIQRFKGLGEMNPEQLWDTTVNPDTRRLLQVCIEDG
CCCCCEEEECCHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHCC
IAADQIFSTLMGDVVEPRRGFIENNALKVTRLDI
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
>Mature Secondary Structure 
TEKQNTSLLSNGGIYDSSKITVLRGLDAVRKRPGMYIGDVHDGTGLHHMVFEVVDNSVD
CCCCCCCEECCCCCCCCCCEEEECCCHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHH
EALAGHADSILVKIHIDGSVSVSDNGRGIPVDIHKEEGVSAAEVILTVLHAGGKFDDNSY
HHHCCCCCEEEEEEEECCEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCE
KVSGGLHGVGVSVVNALSERLWLDIWRDGYHYQQEYVLGEPQYPLKQLEVSAKRGTTLRF
EECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHEECCCCCCCHHHHHHHHCCCCEEEE
KPAKEIFSDVEFHYENLAKRLRELSFLNSGLKVSLIDERGEGRRDDFHYEGGIRSFVEHL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHH
AQLKTPLHPNVISVTGEHNGIVVDAALQWTDAYQETMYCFTNNIPQKDGGTHLAGFRAAL
HHHCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
TRTLGNYIEQNGVARQAKITFSGDDMREGMIAVLSVKVPEPSFSSQTKEKLVSSDVKPAV
HHHHHHHHHHCCCCEEEEEEECCCCHHCCEEEEEEEECCCCCCCHHHHHHHHHCCCCHHH
EATFGLRLEEFLQENPNEARAIAGKIVDAARAREAARKARDLTRRKGVLDIAGLPGKLAD
HHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCC
CQEKDPAMSELFIVEGDSAGGSAKQGRNRKNQAVLPLRGKILNVERARFDRMLSSAEVGT
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHH
LITALGTGIGKDEYNPDKLRYHRVIIMTDADVDGSHIRTLLLTFFYRQMPELIERGHIYI
HHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
GLPPLYKLKQGKSELYLKDDIALNVYLANSAVEGAQLLPAEGEPPIEGLALEKLLITYIA
CCCCHHHHHCCCCCEEEECCEEEEEEEECCCCCCCEECCCCCCCCCHHHHHHHHHHHHHH
AKDTIVRHSHRYDSRLLEALLEFTPLDSTCFQSYSDSEHLQSLMALLNQSSLGAPRYILK
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEE
MQFPDEQHSAALLATRYHMGEELTQVLQLSALETGELRPLYEAAKLLHGLIRNGAKIVRG
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHEEEC
TKSQSIECFGDAQAWLLEEAKKGRQIQRFKGLGEMNPEQLWDTTVNPDTRRLLQVCIEDG
CCCCCEEEECCHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHCC
IAADQIFSTLMGDVVEPRRGFIENNALKVTRLDI
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]