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The map label for this gene is iadA

Identifier: 170757432

GI number: 170757432

Start: 3745957

End: 3747132

Strand: Reverse

Name: iadA

Synonym: CLD_1070

Alternate gene names: 170757432

Gene position: 3747132-3745957 (Counterclockwise)

Preceding gene: 170755667

Following gene: 170754340

Centisome position: 94.67

GC content: 31.72

Gene sequence:

>1176_bases
ATGATAACAATTATAAAAAATGGATATGTATATTCCCCTAAATTTTTAGGGAAAAAAGACATTTTAATAGCTTGTAGTAA
TATAGAAGGGGTATATGATAGTTTAGATATACCGAAGGAATTCGGTGAAATAAAGGTTATAGATGCTAAGGAAAATATAG
TTACACCAGGATTTATAGATTCCCATGTTCATTTAATAGGTGGTGGTGGTGAAGGGGGATTTAGCACTAGAACACCTGAA
ATACAATTATCACATATAATAAGTTCTGGTATAACTACCGTAGTAGGGTGTTTAGGAACAGATGGTATATGTAGAAGTAT
GGAAGCTCTGTTAGCTAAAGCCAAGGGATTAGAAGAAGAGGGCATAACCACATACATATACACTGGATCATATAGCATAC
CAGTTAATAATATCACAGGCTGTTCAAAGTCTGATATTATGCTAATAGATAAAGTTATAGGTGTAGGAGAAATAGCTTTG
TCAGATCATAGATCTTCCCAACCTAGTTATGAGGATTTTGTAAAAATTTGCGCAGAGGGAAGAGTAGGCGGATTATTAGC
TAATAAGGCTGGAGTTATTCATGTTCACATTGGTAGTGGTAAAAAAGGAATAGATTATTTGTTTAAAGTAATAAAAGATA
CAGAAATACCTGCATCTCAAATTATACCTACTCATATGGGAAGAAGTGAAGATTTATTTAACCAAGGGATACAATATATG
AAAAAAGGTGGAGTTATAGATTTAACAACAAGCTCTGATCCTGATTTTTTAGAAGAAGGAGAAATAAAGGCTAGTTCAGG
ACTTAAAAGAATATTAGATAGTGGTTTAGATATAGAAAGAGTTCATTTTTCTTCAGATGGACAGGGAAGCTTGCCCAGAT
TTAACGAAAAAAGAGAATATGTAGGTTTAGGTATAGGTTCAGTACACTCATTATATAGGGAATTTAAGGATTGTGTTGTA
AAAGAAAAAATAAAAATAGAAGATGCACTAAAAGTAGTTACATCAAATGTAGCAGATTATTTGATGCTAAATAATAAAGG
ACATATAGAAAAAGAAAAGGATGCAGATATAGTAATATTAGATAAGGAGAATTTAGAGATACTACATGTTATTTGCAAAG
GTAAACATTTAGTTGAAGATGGTAAAGTGTTAAAGAAAGGCACATTTGAAGGATAA

Upstream 100 bases:

>100_bases
TAAAGAGAATTATAAATAACAATTAATATTTTAGAATATTTTATTTATAATGTAAAACAATAATATTAGTTCTAATTTTG
TTTTACAGGAGAGAATAAAG

Downstream 100 bases:

>100_bases
GTAATAAATTTTTATAATACATTTTAAAACTATTGACTAATATATTTGTATATATTAGAATATAAGTAAATACATACCCT
ATAAGGGTATATCGGAGGGT

Product: isoaspartyl dipeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MITIIKNGYVYSPKFLGKKDILIACSNIEGVYDSLDIPKEFGEIKVIDAKENIVTPGFIDSHVHLIGGGGEGGFSTRTPE
IQLSHIISSGITTVVGCLGTDGICRSMEALLAKAKGLEEEGITTYIYTGSYSIPVNNITGCSKSDIMLIDKVIGVGEIAL
SDHRSSQPSYEDFVKICAEGRVGGLLANKAGVIHVHIGSGKKGIDYLFKVIKDTEIPASQIIPTHMGRSEDLFNQGIQYM
KKGGVIDLTTSSDPDFLEEGEIKASSGLKRILDSGLDIERVHFSSDGQGSLPRFNEKREYVGLGIGSVHSLYREFKDCVV
KEKIKIEDALKVVTSNVADYLMLNNKGHIEKEKDADIVILDKENLEILHVICKGKHLVEDGKVLKKGTFEG

Sequences:

>Translated_391_residues
MITIIKNGYVYSPKFLGKKDILIACSNIEGVYDSLDIPKEFGEIKVIDAKENIVTPGFIDSHVHLIGGGGEGGFSTRTPE
IQLSHIISSGITTVVGCLGTDGICRSMEALLAKAKGLEEEGITTYIYTGSYSIPVNNITGCSKSDIMLIDKVIGVGEIAL
SDHRSSQPSYEDFVKICAEGRVGGLLANKAGVIHVHIGSGKKGIDYLFKVIKDTEIPASQIIPTHMGRSEDLFNQGIQYM
KKGGVIDLTTSSDPDFLEEGEIKASSGLKRILDSGLDIERVHFSSDGQGSLPRFNEKREYVGLGIGSVHSLYREFKDCVV
KEKIKIEDALKVVTSNVADYLMLNNKGHIEKEKDADIVILDKENLEILHVICKGKHLVEDGKVLKKGTFEG
>Mature_391_residues
MITIIKNGYVYSPKFLGKKDILIACSNIEGVYDSLDIPKEFGEIKVIDAKENIVTPGFIDSHVHLIGGGGEGGFSTRTPE
IQLSHIISSGITTVVGCLGTDGICRSMEALLAKAKGLEEEGITTYIYTGSYSIPVNNITGCSKSDIMLIDKVIGVGEIAL
SDHRSSQPSYEDFVKICAEGRVGGLLANKAGVIHVHIGSGKKGIDYLFKVIKDTEIPASQIIPTHMGRSEDLFNQGIQYM
KKGGVIDLTTSSDPDFLEEGEIKASSGLKRILDSGLDIERVHFSSDGQGSLPRFNEKREYVGLGIGSVHSLYREFKDCVV
KEKIKIEDALKVVTSNVADYLMLNNKGHIEKEKDADIVILDKENLEILHVICKGKHLVEDGKVLKKGTFEG

Specific function: Catalyzes the hydrolytic cleavage of a subset of L- isoaspartyl (L-beta-aspartyl) dipeptides. Used to degrade proteins damaged by L-isoaspartyl residues formation. The best substrate for the enzyme reported thus far is iso-Asp-Leu [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M38 family [H]

Homologues:

Organism=Escherichia coli, GI1790784, Length=390, Percent_Identity=40.5128205128205, Blast_Score=300, Evalue=9e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011059
- InterPro:   IPR010229 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.4.19.- [C]

Molecular weight: Translated: 42518; Mature: 42518

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIIKNGYVYSPKFLGKKDILIACSNIEGVYDSLDIPKEFGEIKVIDAKENIVTPGFID
CEEEEECCEEECCCCCCCCCEEEEECCCCCCHHCCCCCHHCCCEEEEECCCCCCCCCEEC
SHVHLIGGGGEGGFSTRTPEIQLSHIISSGITTVVGCLGTDGICRSMEALLAKAKGLEEE
CEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
GITTYIYTGSYSIPVNNITGCSKSDIMLIDKVIGVGEIALSDHRSSQPSYEDFVKICAEG
CCEEEEEECEEEECCCCCCCCCCCCEEEEEHHHCCCCEEECCCCCCCCCHHHHHHHHHCC
RVGGLLANKAGVIHVHIGSGKKGIDYLFKVIKDTEIPASQIIPTHMGRSEDLFNQGIQYM
CCCEEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHHCCHHCCCCCHHHHHHHHHHH
KKGGVIDLTTSSDPDFLEEGEIKASSGLKRILDSGLDIERVHFSSDGQGSLPRFNEKREY
HCCCEEEEECCCCCCCHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCE
VGLGIGSVHSLYREFKDCVVKEKIKIEDALKVVTSNVADYLMLNNKGHIEKEKDADIVIL
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEEE
DKENLEILHVICKGKHLVEDGKVLKKGTFEG
ECCCCEEEEEECCCCEEECCCCEECCCCCCC
>Mature Secondary Structure
MITIIKNGYVYSPKFLGKKDILIACSNIEGVYDSLDIPKEFGEIKVIDAKENIVTPGFID
CEEEEECCEEECCCCCCCCCEEEEECCCCCCHHCCCCCHHCCCEEEEECCCCCCCCCEEC
SHVHLIGGGGEGGFSTRTPEIQLSHIISSGITTVVGCLGTDGICRSMEALLAKAKGLEEE
CEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
GITTYIYTGSYSIPVNNITGCSKSDIMLIDKVIGVGEIALSDHRSSQPSYEDFVKICAEG
CCEEEEEECEEEECCCCCCCCCCCCEEEEEHHHCCCCEEECCCCCCCCCHHHHHHHHHCC
RVGGLLANKAGVIHVHIGSGKKGIDYLFKVIKDTEIPASQIIPTHMGRSEDLFNQGIQYM
CCCEEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHHCCHHCCCCCHHHHHHHHHHH
KKGGVIDLTTSSDPDFLEEGEIKASSGLKRILDSGLDIERVHFSSDGQGSLPRFNEKREY
HCCCEEEEECCCCCCCHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCE
VGLGIGSVHSLYREFKDCVVKEKIKIEDALKVVTSNVADYLMLNNKGHIEKEKDADIVIL
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEEE
DKENLEILHVICKGKHLVEDGKVLKKGTFEG
ECCCCEEEEEECCCCEEECCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7876157; 7610040; 9278503 [H]