| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is adhE
Identifier: 170756566
GI number: 170756566
Start: 426036
End: 428624
Strand: Direct
Name: adhE
Synonym: CLD_0407
Alternate gene names: 170756566
Gene position: 426036-428624 (Clockwise)
Preceding gene: 170757630
Following gene: 170757136
Centisome position: 10.76
GC content: 32.6
Gene sequence:
>2589_bases ATGAAAATTACCACTACTGAGGAACTAATAAGAAAAATTGAAAAAATCAAAGAGGCACAAAAGATTTATTCCACATATTC TCAAGATAAGGTAGACAAAATATTTAAAGCAGCAGCTATAGCAGCTAACAAAGAAAGAATAAAACTTGCAAAAATGGCAG TAGAAGAAACGGGCATGGGTATTGTAGAAGATAAAGTTATAAAAAATCATTTTGCATCTGAATATATTTATAACAAATAT AAAGATGAAAAAACCTGTGGTGTAATAGAAAAGGATGAAGCCTTTGGGTTAACAAAAATTGCAGAACCAATAGGTGTTAT TGCGGCAATAGTTCCAACGACTAATCCAACCTCAACAGCTATTTTCAAAGCTTTAATAGCTTTAAAAACTAGAAATGGTA TAATTTTTTCACCTCATCCAAGAGCTAAAAAATCTACAATAATGGCAGCTAAGATTGTTTTAGATGCAGCAGTACAAGCA GGGGCGCCAAAAGAAATAATAGGTTGGATAGATGAACCTACCTTAGAATTGTCTAATGCAGTAATGAGTAATTCAAATCT AGTTCTTGCAACAGGTGGACCAGGTATGGTTAAAGCGGCATACTCTTCAGGAAAGCCAGCTATAGGAGTAGGACCTGGAA ATGTACCAGCTATAATACACGAAACAGCAGATATTAAAATGGCTGTAAGCTCAGTGGTACTTTCTAAAACTTTTGATAAT GGTATGATTTGTGCATCAGAACAATCAGTAATTGTAATGAATAGCATATATGAAGAAGTAAAAAAAGAATTTGTAATTAG AGGAGCCTATGTATTAAATAAAGAAGAGATAGAAAAAGTTAAGAAAATAATTTTAGTTAATGGAAATGTAAATGCTAAAA TTGTTGGTCAAACACCACAAAAGATAGGTGAAATGGCCGGAATAAAAGTTCCAGATTGGGCTAAACTTTTAGTTGGAGAA GTTCAATCTGTAGAATTAGAAGAACCATTCTCTCACGAAAAACTATCACCAGTTTTAGCAATGTATAAAGTTAAAACTTA TGAAGAGGCTTTAACTAAAGCTGAAAGATTAGTAGAATTAGGAGGATTTGGACATACATCTTCATTGTATATAAACACAA TAAAATGCAAAGAAGAAGTAGAAAAGTTCTCAAATAATATGAAAACAGGAAGAACAATTATAAATATGCCATCAGCCCAA GGTGGTATAGGTGATATATATAACTTTAAATTAGCACCATCTCTAACTCTTGGTTGTGGATCCTGGGGTGGAAACTCAGT ATCAGAGAATGTAGGACCAAAACACTTATTAAATATCAAAAATGTAGCTGAGAGGAGAGAGAATATGCTTTGGTTTAGAG TACCAGAAAAAGTTTACTTTAAATATGGATGTCTTCCAATAGCTTTAAAAGAATTAAAAAGAATGAACAAGAAAAAGGCA TTTATAGTTACAGATAAAGTATTATATGAATTAGGAGTTGCTAAAAAAGCAACAGATGTGTTGGATGAGATAGGAATAAA TTATAAGGTATTCTTTGATGTAGCACCAGATCCAACTTTAGAAACAGCAAAAAAAGGTGCAAAAGAAATGGTAGATTTTA ATCCTGATACAATAATAGCTATAGGCGGAGGATCTGCTATGGATGCTGCTAAAATAATGTGGGTAATGTATGAACATCCA GAAGTAGAATTCGAAGATTTAGCTATGAGATTTATGGATATAAGAAAAAGAGTATATGAGTTCCCACATATGGGAGATAA AGCAATGATGATATCAGTAGCTACTTCAGCAGGTACTGGTTCAGAAGTAACTCCTTTTGCTGTTATAACTGATGAAAAAA CAGGTGTGAAATATCCACTAGCAGATTATGAATTAACTCCAGATATGGCTATAGTAGATGCAGATTTAATGCTTAATATG CCAAAGGGATTAACTGCTGCATCAGGAATTGATGCATTAACTCATGCAGTAGAAGCTTATGTATCAGTTATGGCATCAGA GTATACAGATGGACTATGTTTAGAGGCTATAAAAACCATATTTGAATATCTACCAAAAGCTTATAAAGAAGGAGCCCAAG ATATAGAAGCAAGAGAAAAAATGGCTCATGCATCAACTATAGCAGGTATGGCTTTTGCAAATGCCTTCTTAGGAGTATGC CACTCTATGGCTCATAAATTAGGATCTATGCATCATGTACCACATGGTATAGCTAATGCTTTATTAATAAATGAAACAAT TAAATTTAATTCTGAGGATATGCCAAGAAAGCAAACTGCTTTCCCACAATATAAATATCCAAATGCTAAGGCTAAATATG CTAATATAGCAGATTATTTATCATTAGGTGGAAAAACTCCAGAAGAAAAAGTTGAGCTATTAATAAAAGCTATAGATAAA TTAAAAGCAGAAGTAAATATTCCAACCTCTATAGAAGAGGCAGGGGTATCAAAGGATAAATTCTTTAAAACTTTAGATGA GATGTCCGAACAAGCTTTTGATGATCAATGTACAGGAGCTAATCCAAGATACCCATTAATAAGTGAAATAAAAGAAATGT ATACTAATGTTTTTAGTACCAAAAAATAG
Upstream 100 bases:
>100_bases AGAAGTTATAAGGTATTAGTAATATTAATATAAATTAATACTTTTTATATTTTAGATAAGTTTGTTAAAAAATAAACTAT TATTAAAAGAGGTGTTATTA
Downstream 100 bases:
>100_bases GATAGTTTAATTTATATAGCATAAAATATTATTATATATATTTTATAGAAAGCTAAAATTTTAATTTTACCCAAAACTAA ACCATTTATACAAAAAATAA
Product: bifunctional acetaldehyde-CoA/alcohol dehydrogenase
Products: NA
Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH [H]
Number of amino acids: Translated: 862; Mature: 862
Protein sequence:
>862_residues MKITTTEELIRKIEKIKEAQKIYSTYSQDKVDKIFKAAAIAANKERIKLAKMAVEETGMGIVEDKVIKNHFASEYIYNKY KDEKTCGVIEKDEAFGLTKIAEPIGVIAAIVPTTNPTSTAIFKALIALKTRNGIIFSPHPRAKKSTIMAAKIVLDAAVQA GAPKEIIGWIDEPTLELSNAVMSNSNLVLATGGPGMVKAAYSSGKPAIGVGPGNVPAIIHETADIKMAVSSVVLSKTFDN GMICASEQSVIVMNSIYEEVKKEFVIRGAYVLNKEEIEKVKKIILVNGNVNAKIVGQTPQKIGEMAGIKVPDWAKLLVGE VQSVELEEPFSHEKLSPVLAMYKVKTYEEALTKAERLVELGGFGHTSSLYINTIKCKEEVEKFSNNMKTGRTIINMPSAQ GGIGDIYNFKLAPSLTLGCGSWGGNSVSENVGPKHLLNIKNVAERRENMLWFRVPEKVYFKYGCLPIALKELKRMNKKKA FIVTDKVLYELGVAKKATDVLDEIGINYKVFFDVAPDPTLETAKKGAKEMVDFNPDTIIAIGGGSAMDAAKIMWVMYEHP EVEFEDLAMRFMDIRKRVYEFPHMGDKAMMISVATSAGTGSEVTPFAVITDEKTGVKYPLADYELTPDMAIVDADLMLNM PKGLTAASGIDALTHAVEAYVSVMASEYTDGLCLEAIKTIFEYLPKAYKEGAQDIEAREKMAHASTIAGMAFANAFLGVC HSMAHKLGSMHHVPHGIANALLINETIKFNSEDMPRKQTAFPQYKYPNAKAKYANIADYLSLGGKTPEEKVELLIKAIDK LKAEVNIPTSIEEAGVSKDKFFKTLDEMSEQAFDDQCTGANPRYPLISEIKEMYTNVFSTKK
Sequences:
>Translated_862_residues MKITTTEELIRKIEKIKEAQKIYSTYSQDKVDKIFKAAAIAANKERIKLAKMAVEETGMGIVEDKVIKNHFASEYIYNKY KDEKTCGVIEKDEAFGLTKIAEPIGVIAAIVPTTNPTSTAIFKALIALKTRNGIIFSPHPRAKKSTIMAAKIVLDAAVQA GAPKEIIGWIDEPTLELSNAVMSNSNLVLATGGPGMVKAAYSSGKPAIGVGPGNVPAIIHETADIKMAVSSVVLSKTFDN GMICASEQSVIVMNSIYEEVKKEFVIRGAYVLNKEEIEKVKKIILVNGNVNAKIVGQTPQKIGEMAGIKVPDWAKLLVGE VQSVELEEPFSHEKLSPVLAMYKVKTYEEALTKAERLVELGGFGHTSSLYINTIKCKEEVEKFSNNMKTGRTIINMPSAQ GGIGDIYNFKLAPSLTLGCGSWGGNSVSENVGPKHLLNIKNVAERRENMLWFRVPEKVYFKYGCLPIALKELKRMNKKKA FIVTDKVLYELGVAKKATDVLDEIGINYKVFFDVAPDPTLETAKKGAKEMVDFNPDTIIAIGGGSAMDAAKIMWVMYEHP EVEFEDLAMRFMDIRKRVYEFPHMGDKAMMISVATSAGTGSEVTPFAVITDEKTGVKYPLADYELTPDMAIVDADLMLNM PKGLTAASGIDALTHAVEAYVSVMASEYTDGLCLEAIKTIFEYLPKAYKEGAQDIEAREKMAHASTIAGMAFANAFLGVC HSMAHKLGSMHHVPHGIANALLINETIKFNSEDMPRKQTAFPQYKYPNAKAKYANIADYLSLGGKTPEEKVELLIKAIDK LKAEVNIPTSIEEAGVSKDKFFKTLDEMSEQAFDDQCTGANPRYPLISEIKEMYTNVFSTKK >Mature_862_residues MKITTTEELIRKIEKIKEAQKIYSTYSQDKVDKIFKAAAIAANKERIKLAKMAVEETGMGIVEDKVIKNHFASEYIYNKY KDEKTCGVIEKDEAFGLTKIAEPIGVIAAIVPTTNPTSTAIFKALIALKTRNGIIFSPHPRAKKSTIMAAKIVLDAAVQA GAPKEIIGWIDEPTLELSNAVMSNSNLVLATGGPGMVKAAYSSGKPAIGVGPGNVPAIIHETADIKMAVSSVVLSKTFDN GMICASEQSVIVMNSIYEEVKKEFVIRGAYVLNKEEIEKVKKIILVNGNVNAKIVGQTPQKIGEMAGIKVPDWAKLLVGE VQSVELEEPFSHEKLSPVLAMYKVKTYEEALTKAERLVELGGFGHTSSLYINTIKCKEEVEKFSNNMKTGRTIINMPSAQ GGIGDIYNFKLAPSLTLGCGSWGGNSVSENVGPKHLLNIKNVAERRENMLWFRVPEKVYFKYGCLPIALKELKRMNKKKA FIVTDKVLYELGVAKKATDVLDEIGINYKVFFDVAPDPTLETAKKGAKEMVDFNPDTIIAIGGGSAMDAAKIMWVMYEHP EVEFEDLAMRFMDIRKRVYEFPHMGDKAMMISVATSAGTGSEVTPFAVITDEKTGVKYPLADYELTPDMAIVDADLMLNM PKGLTAASGIDALTHAVEAYVSVMASEYTDGLCLEAIKTIFEYLPKAYKEGAQDIEAREKMAHASTIAGMAFANAFLGVC HSMAHKLGSMHHVPHGIANALLINETIKFNSEDMPRKQTAFPQYKYPNAKAKYANIADYLSLGGKTPEEKVELLIKAIDK LKAEVNIPTSIEEAGVSKDKFFKTLDEMSEQAFDDQCTGANPRYPLISEIKEMYTNVFSTKK
Specific function: This enzyme has probably two activities:ADH, and ACDH [H]
COG id: COG1012
COG function: function code C; NAD-dependent aldehyde dehydrogenases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]
Homologues:
Organism=Homo sapiens, GI133922590, Length=327, Percent_Identity=28.4403669724771, Blast_Score=99, Evalue=2e-20, Organism=Escherichia coli, GI1787493, Length=862, Percent_Identity=62.4129930394432, Blast_Score=1109, Evalue=0.0, Organism=Escherichia coli, GI1789163, Length=405, Percent_Identity=34.320987654321, Blast_Score=231, Evalue=1e-61, Organism=Escherichia coli, GI48994951, Length=406, Percent_Identity=35.7142857142857, Blast_Score=219, Evalue=7e-58, Organism=Escherichia coli, GI87082107, Length=403, Percent_Identity=31.5136476426799, Blast_Score=199, Evalue=4e-52, Organism=Escherichia coli, GI1788797, Length=375, Percent_Identity=32.5333333333333, Blast_Score=166, Evalue=8e-42, Organism=Escherichia coli, GI1789386, Length=405, Percent_Identity=23.9506172839506, Blast_Score=76, Evalue=9e-15, Organism=Caenorhabditis elegans, GI17537053, Length=328, Percent_Identity=25.609756097561, Blast_Score=107, Evalue=2e-23, Organism=Saccharomyces cerevisiae, GI6321181, Length=401, Percent_Identity=33.9152119700748, Blast_Score=216, Evalue=2e-56, Organism=Saccharomyces cerevisiae, GI6323821, Length=371, Percent_Identity=22.911051212938, Blast_Score=65, Evalue=4e-11, Organism=Drosophila melanogaster, GI24657991, Length=324, Percent_Identity=26.5432098765432, Blast_Score=87, Evalue=6e-17,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001670 - InterPro: IPR018211 - InterPro: IPR016161 - InterPro: IPR016163 - InterPro: IPR016162 - InterPro: IPR015590 - InterPro: IPR012079 [H]
Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]
EC number: =1.1.1.1; =1.2.1.10 [H]
Molecular weight: Translated: 94666; Mature: 94666
Theoretical pI: Translated: 7.06; Mature: 7.06
Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 4.1 %Met (Translated Protein) 5.0 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 4.1 %Met (Mature Protein) 5.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKITTTEELIRKIEKIKEAQKIYSTYSQDKVDKIFKAAAIAANKERIKLAKMAVEETGMG CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC IVEDKVIKNHFASEYIYNKYKDEKTCGVIEKDEAFGLTKIAEPIGVIAAIVPTTNPTSTA HHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHEEEECCCCCHHHH IFKALIALKTRNGIIFSPHPRAKKSTIMAAKIVLDAAVQAGAPKEIIGWIDEPTLELSNA HHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHH VMSNSNLVLATGGPGMVKAAYSSGKPAIGVGPGNVPAIIHETADIKMAVSSVVLSKTFDN HCCCCCEEEEECCCCCEEEECCCCCCEEEECCCCCCEEEECCCHHHHHHHHHHHHHHCCC GMICASEQSVIVMNSIYEEVKKEFVIRGAYVLNKEEIEKVKKIILVNGNVNAKIVGQTPQ CEEEECCCCEEEHHHHHHHHHHHHHHHHHEEECHHHHHHHHEEEEECCCCCEEEECCCHH KIGEMAGIKVPDWAKLLVGEVQSVELEEPFSHEKLSPVLAMYKVKTYEEALTKAERLVEL HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH GGFGHTSSLYINTIKCKEEVEKFSNNMKTGRTIINMPSAQGGIGDIYNFKLAPSLTLGCG CCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEECC SWGGNSVSENVGPKHLLNIKNVAERRENMLWFRVPEKVYFKYGCLPIALKELKRMNKKKA CCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCHHHHHHHCCHHHHHHHHHHHCCCCE FIVTDKVLYELGVAKKATDVLDEIGINYKVFFDVAPDPTLETAKKGAKEMVDFNPDTIIA EEEEHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEE IGGGSAMDAAKIMWVMYEHPEVEFEDLAMRFMDIRKRVYEFPHMGDKAMMISVATSAGTG ECCCCHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCC SEVTPFAVITDEKTGVKYPLADYELTPDMAIVDADLMLNMPKGLTAASGIDALTHAVEAY CCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEHHHHEECCCCCCHHHHHHHHHHHHHHH VSVMASEYTDGLCLEAIKTIFEYLPKAYKEGAQDIEAREKMAHASTIAGMAFANAFLGVC HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HSMAHKLGSMHHVPHGIANALLINETIKFNSEDMPRKQTAFPQYKYPNAKAKYANIADYL HHHHHHHCCCCCCCHHHHHHHHEECCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHH SLGGKTPEEKVELLIKAIDKLKAEVNIPTSIEEAGVSKDKFFKTLDEMSEQAFDDQCTGA HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC NPRYPLISEIKEMYTNVFSTKK CCCCCHHHHHHHHHHHHHCCCC >Mature Secondary Structure MKITTTEELIRKIEKIKEAQKIYSTYSQDKVDKIFKAAAIAANKERIKLAKMAVEETGMG CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC IVEDKVIKNHFASEYIYNKYKDEKTCGVIEKDEAFGLTKIAEPIGVIAAIVPTTNPTSTA HHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHEEEECCCCCHHHH IFKALIALKTRNGIIFSPHPRAKKSTIMAAKIVLDAAVQAGAPKEIIGWIDEPTLELSNA HHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHH VMSNSNLVLATGGPGMVKAAYSSGKPAIGVGPGNVPAIIHETADIKMAVSSVVLSKTFDN HCCCCCEEEEECCCCCEEEECCCCCCEEEECCCCCCEEEECCCHHHHHHHHHHHHHHCCC GMICASEQSVIVMNSIYEEVKKEFVIRGAYVLNKEEIEKVKKIILVNGNVNAKIVGQTPQ CEEEECCCCEEEHHHHHHHHHHHHHHHHHEEECHHHHHHHHEEEEECCCCCEEEECCCHH KIGEMAGIKVPDWAKLLVGEVQSVELEEPFSHEKLSPVLAMYKVKTYEEALTKAERLVEL HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH GGFGHTSSLYINTIKCKEEVEKFSNNMKTGRTIINMPSAQGGIGDIYNFKLAPSLTLGCG CCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEEEEECCCEEEECC SWGGNSVSENVGPKHLLNIKNVAERRENMLWFRVPEKVYFKYGCLPIALKELKRMNKKKA CCCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCHHHHHHHCCHHHHHHHHHHHCCCCE FIVTDKVLYELGVAKKATDVLDEIGINYKVFFDVAPDPTLETAKKGAKEMVDFNPDTIIA EEEEHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEE IGGGSAMDAAKIMWVMYEHPEVEFEDLAMRFMDIRKRVYEFPHMGDKAMMISVATSAGTG ECCCCHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCC SEVTPFAVITDEKTGVKYPLADYELTPDMAIVDADLMLNMPKGLTAASGIDALTHAVEAY CCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEHHHHEECCCCCCHHHHHHHHHHHHHHH VSVMASEYTDGLCLEAIKTIFEYLPKAYKEGAQDIEAREKMAHASTIAGMAFANAFLGVC HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH HSMAHKLGSMHHVPHGIANALLINETIKFNSEDMPRKQTAFPQYKYPNAKAKYANIADYL HHHHHHHCCCCCCCHHHHHHHHEECCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHH SLGGKTPEEKVELLIKAIDKLKAEVNIPTSIEEAGVSKDKFFKTLDEMSEQAFDDQCTGA HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC NPRYPLISEIKEMYTNVFSTKK CCCCCHHHHHHHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8226639; 8300540; 11466286 [H]