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The map label for this gene is birA

Identifier: 170754620

GI number: 170754620

Start: 1242676

End: 1243659

Strand: Reverse

Name: birA

Synonym: CLD_3444

Alternate gene names: 170754620

Gene position: 1243659-1242676 (Counterclockwise)

Preceding gene: 170754854

Following gene: 170756286

Centisome position: 31.42

GC content: 26.73

Gene sequence:

>984_bases
TTGAAAGAAGCTATAATAAATATATTAAAAAATAGTTCCCATACATTTATATCTGGACAATATATTAGTGATAAGTTAGG
TGTTTCAAGAACAGCCATTTGGAAATATATAAATGGACTAAAAAAAGAAGGTTATAATATAGAATCTGCATCAAAAAAAG
GATATAGATTAATTTCTTGTCCTGATATTTTGACTTTTGAAGAAATTATAAGTCTATTAAAAACTAAGGTTATAGGCCAA
AATATTATTCATTTTGACTCTATTAATTCTACTAATGACAAAGCAAAAAAAATAGCCTCTTCTGAAAATGATGGTACAAT
AATAATATCTGAAGAACAAACTCTAGGGAAGGGGCGCTTAGGTAGAACTTGGTTCTCTTCCAAATTTAAAGGTATCTATA
TGTCCATTATATTAAAGCCAGACATAAACACTATGGATGTCCCTAAAATAACTCAAATAGCCGCTGCTGCTGTATTAACA
GCTTTACTTAATAATAATATTAAAACTTATATAAAATGGCCTAATGATATTATAGTAAATAATAAAAAAGTATGTGGTAT
TTTAACAGAAATGAGCGGAGAGATAAGCAAAGTAAATTATGTAGTAGTGGGTATGGGCATAAATGTAAATTGTGAAAAAG
AAGATTTACCAAAGGATTTACTTTCAAAGGCAACATCTTTAAAAATAGAAAAAGACAGAGAATTTAAAAGAAATATTTTA
GTTGCAGATATACTAAATAATTTTGAAAACCTATATACGGAACTTATAGAATGTAACAATATAGATACTTCTATAGAAAT
ATGTAAAGAAAATTCTATACTAATTGGAAAAGAAGTTAGAATAATAAACAAAAATAGAGAAAGCTTTGGAAAAGCTGTAG
GTTTAAACTCTAATGGTGAACTTTTAGTTCAATTTGAAAATGGAGAAATAAAACCACTAATTTCTGGAGAAATATCTGTA
AGAGGAATGAATGGATACATATAA

Upstream 100 bases:

>100_bases
TGATAAAACAATTTAAGTTTAAAATATTATATTATAAATATTTTTATGTAGTATAATATAATAAAGAAACTTTTAGTTAT
AAACTAAGGAGGCAAATAGT

Downstream 100 bases:

>100_bases
GGAAATTGTGACTTAAAGTAAGTTATCCACAGCCTATTAAAAAGGGATATGTGGCTACAAAGGCGTCTCAATTCGCTAAG
AGATTCTAATTTGCTTTTTC

Product: birA bifunctional protein

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKEAIINILKNSSHTFISGQYISDKLGVSRTAIWKYINGLKKEGYNIESASKKGYRLISCPDILTFEEIISLLKTKVIGQ
NIIHFDSINSTNDKAKKIASSENDGTIIISEEQTLGKGRLGRTWFSSKFKGIYMSIILKPDINTMDVPKITQIAAAAVLT
ALLNNNIKTYIKWPNDIIVNNKKVCGILTEMSGEISKVNYVVVGMGINVNCEKEDLPKDLLSKATSLKIEKDREFKRNIL
VADILNNFENLYTELIECNNIDTSIEICKENSILIGKEVRIINKNRESFGKAVGLNSNGELLVQFENGEIKPLISGEISV
RGMNGYI

Sequences:

>Translated_327_residues
MKEAIINILKNSSHTFISGQYISDKLGVSRTAIWKYINGLKKEGYNIESASKKGYRLISCPDILTFEEIISLLKTKVIGQ
NIIHFDSINSTNDKAKKIASSENDGTIIISEEQTLGKGRLGRTWFSSKFKGIYMSIILKPDINTMDVPKITQIAAAAVLT
ALLNNNIKTYIKWPNDIIVNNKKVCGILTEMSGEISKVNYVVVGMGINVNCEKEDLPKDLLSKATSLKIEKDREFKRNIL
VADILNNFENLYTELIECNNIDTSIEICKENSILIGKEVRIINKNRESFGKAVGLNSNGELLVQFENGEIKPLISGEISV
RGMNGYI
>Mature_327_residues
MKEAIINILKNSSHTFISGQYISDKLGVSRTAIWKYINGLKKEGYNIESASKKGYRLISCPDILTFEEIISLLKTKVIGQ
NIIHFDSINSTNDKAKKIASSENDGTIIISEEQTLGKGRLGRTWFSSKFKGIYMSIILKPDINTMDVPKITQIAAAAVLT
ALLNNNIKTYIKWPNDIIVNNKKVCGILTEMSGEISKVNYVVVGMGINVNCEKEDLPKDLLSKATSLKIEKDREFKRNIL
VADILNNFENLYTELIECNNIDTSIEICKENSILIGKEVRIINKNRESFGKAVGLNSNGELLVQFENGEIKPLISGEISV
RGMNGYI

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=320, Percent_Identity=28.125, Blast_Score=124, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24644246, Length=266, Percent_Identity=24.812030075188, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 36474; Mature: 36474

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEAIINILKNSSHTFISGQYISDKLGVSRTAIWKYINGLKKEGYNIESASKKGYRLISC
CHHHHHHHHHCCCCEEEECEEECHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEC
PDILTFEEIISLLKTKVIGQNIIHFDSINSTNDKAKKIASSENDGTIIISEEQTLGKGRL
CCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCC
GRTWFSSKFKGIYMSIILKPDINTMDVPKITQIAAAAVLTALLNNNIKTYIKWPNDIIVN
CCHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEC
NKKVCGILTEMSGEISKVNYVVVGMGINVNCEKEDLPKDLLSKATSLKIEKDREFKRNIL
CCCEEEEEECCCCCCEEEEEEEEEECEEECCCHHHCCHHHHHHHHCEEEECCHHHHHHHH
VADILNNFENLYTELIECNNIDTSIEICKENSILIGKEVRIINKNRESFGKAVGLNSNGE
HHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECEEEEEECCHHHHHHEECCCCCCC
LLVQFENGEIKPLISGEISVRGMNGYI
EEEEECCCCCCEEECCEEEEEECCCCC
>Mature Secondary Structure
MKEAIINILKNSSHTFISGQYISDKLGVSRTAIWKYINGLKKEGYNIESASKKGYRLISC
CHHHHHHHHHCCCCEEEECEEECHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEC
PDILTFEEIISLLKTKVIGQNIIHFDSINSTNDKAKKIASSENDGTIIISEEQTLGKGRL
CCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCC
GRTWFSSKFKGIYMSIILKPDINTMDVPKITQIAAAAVLTALLNNNIKTYIKWPNDIIVN
CCHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEC
NKKVCGILTEMSGEISKVNYVVVGMGINVNCEKEDLPKDLLSKATSLKIEKDREFKRNIL
CCCEEEEEECCCCCCEEEEEEEEEECEEECCCHHHCCHHHHHHHHCEEEECCHHHHHHHH
VADILNNFENLYTELIECNNIDTSIEICKENSILIGKEVRIINKNRESFGKAVGLNSNGE
HHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECEEEEEECCHHHHHHEECCCCCCC
LLVQFENGEIKPLISGEISVRGMNGYI
EEEEECCCCCCEEECCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]