| Definition | Methylobacterium radiotolerans JCM 2831 chromosome, complete genome. |
|---|---|
| Accession | NC_010505 |
| Length | 6,077,833 |
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The map label for this gene is cheR [C]
Identifier: 170752077
GI number: 170752077
Start: 6033390
End: 6035951
Strand: Direct
Name: cheR [C]
Synonym: Mrad2831_5709
Alternate gene names: 170752077
Gene position: 6033390-6035951 (Clockwise)
Preceding gene: 170752076
Following gene: 170752078
Centisome position: 99.27
GC content: 67.45
Gene sequence:
>2562_bases ATGCTGGGACCTGTTGCACGGCGCGGCATTGATCCGAGGGCTAAGAACGCGGTGATAGACCAGGACCACTTTCCGATCGT CGGCATCGGCGCGTCCGCGGGCGGCATCGAGGCGATGCGGGGCTTTTTCCGCGGGGTGCCGGAGAGGCTGGAGGCGGCCT TCGTCGTCGTCACGCATCTCGGCCGGGAGCACAAGAGCCTGCTCCCGGACGTGCTGGCCCGGCTCACGCCGCTCAAGGTC GAAGCCGCCGCCGACGGCGTCCCGGTGGGGCCGGGCACCGTCTACGTCATGCCATCCGGTTTCGTCATGGGCATCGCGGG CGGGCGGCTGACGTTGACCCCGCTTGGGGAGGGCCGCGAGACCAAGCCGATCGACATCTTCCTGACCGCCCTGGCCCTCG ACCAGGGCGAGCGTGCGGTCGGTGTGATCCTGTCCGGCGGCGACGGCGACGGCGCCATCGGCATCAAGGCGATCAAGGAG CACGGTGGCCTGACCCTGGCCCAGACCGCGGATGGCTACGGCCCCGAGACCGCCGACATGCCGCTCAGCTCGCTGCGGAC CGGCTTCGTTGATTTCGGCGAGACGGCCGAACGGATGGGCGACCGGATCGCCGCGCACGTCGCCGCCGCGCCGATCGCAC CGGATGCGCAGGCCGACGGGGTCGCGCGGGAGTTCGAGGCCGAGCTCCTCACCGAGATCTTCGCCATCCTCCGCCGGCAG GTCGGCCACGACTTCTCGGGCTACAAGCCAAGCACCTTCGGACGCCGCCTGCAGCGGCGCATCGGCGTCGTCGGCGCGTC CGGACTGGACGGCTATCTCAAGCTGCTGCGGGCTGACCCGGCCGAGGTTGGGGTGCTGTTCCGGGACTTGCTGATCGGTG TGACAAACTTCTTCCGCGATACCGCTGCCTTCGAGGCCCTGACCGCCCAGGTCATCCCAAAGCTGTTCGACGCACGCGCG GCGACCGATGTCATACGGATTTGGGTGCCGGCCTGCTCGACCGGTGAGGAGGTCTACTCTCTGGCCATCCTCCTGCGCGA GCACATGGTCACCCTGGCGGATCCGCCGCGCGTGCAGATCTTCGCCACTGACATCGACGAGCGCGCGCTCACGGTCGCCC GGACTGGCCGCTACCCGACAGCCTACCTGTCCGCCGTCTCGCCTGAGCGGATCGGGCAGCACTTCGTCGTCGAGGGCGAC AGCGCGGTGGTCGAGAAGGCCGTGCGCGACCTGTGCACCTTTGCCCCTCACAATGTGCTGCGCGACCCGCCGTTCTCGCG CCTCGATCTCGTGTCCTGCCGCAACCTGATGATCTACCTCGGCGTCGAGGCCCAGCAACAGCTGATGCCGGTTCTGCACT ACGCCCTGCGCCCTCGCGGCTATTTGTTCATCGGCATGGCCGAGAACGTGACGCGGTTCGAGGACCTGTTCGAGACGATC GACAAGCAGCACCGCATCTTCCAGGCTCGCGACGCGATCCCGCCGGCCCGGTTGCGGCCGATGTCCGGGACGGATACGCC AATCCTTGCAAATGCGGGCCACCCGCAGCGGCGCAACGTGACGACGCACGCCGCCCTGCGGCACGCGGTCGAGACGCAGA TGCTGTCCGAGTTCACGCCGCCCCACGCGGTCGTGACGCGGACGGGCGAGGCCGTGCACTACTCATCGCGGATCGGCGAT CACCTGGAGGTCGTGCCCGGCCAGCCGACCCGGGAGCTGGCTGCGATGGCGCGTAAAGGCCTGCGGCTGGATCTCCGGAC TGCCTTGCGCGAGGCGATCGAGGGCAACATTCTGGTCGTGCGCGAAGGCATCGGCGTCGAGCTGCCGGACGGCCGTTTCC AGTCGATCTCCCTTGTGGTCAAGCCGCTGAACACGCCCGGCGCGGTCGAGCCGCTGTTCCTGGTCGTGTTCAACGAGGCG GCCGCGCCGGTCGAGAGGGAGCGGCAGCAGGCCCTGAAGGCGAACGAGCGGGACACGGCGCTCCAGGGCCTGGAGCGCGA ATTGAGCGTGACGCGCGAACGGCTCCAGGCCGTGATCGAGGAGTATGAGACGTCCCTTGAGGAGCTAAAATCTTCGAACG AAGAACTTTACTCCGTCAACGAAGAGATGCAGGCCGCCAACGAGGAACTTGAGGCTTCGAAGGAGGAGACACAGTCTCTG AATGAGGAGCTTCAGACAATGAATTCCGAGCTCGCGTCCAAGATCGAGGCCGTCGATCAGTTGACCGACGATCAGGCCAC TCTGTTCGAGGGCATGAACATCGCGAGCGTGCTCCTGACACCGGATCTCCACATCCGCATGTTCACCAACGCAGCGGCGC AGATCTTCGGCTTGCAGCCGAGCGACGTCGGCCGGTCCTTGCGCAACTTTTCGGCGCCGATCCCCCTGGCATGGCTGGTG GACGAGATCCCGACAGTGCTGGCGGCGAACCGTCCCTCCGAGCGGACGATCGAGGGGGCGGACGGCGCCCGCTACCGGGT CCGGTTGATGGCCTCGTCCCGCAAGGGCGGCCGGAGGCCCGGGATCGTCGCCAACTTCACGAACCTGCTGGAGCAGGAAT GA
Upstream 100 bases:
>100_bases GCCCCGAGGCTCGGGCCCGCTGCCTCGCCTACGTGGTGCAGGAAGCCACGCACGACAGCTGATCCGCGCAGCCGTCCTCG ACATCGGGCCAGAACACGTG
Downstream 100 bases:
>100_bases CCGACGGATCTGCCCACCTCGCGTGGGATGTTGGCGGCCAGCACGCGCTGGTGCGATCGGAGATGCTCAACAGCGTCAGC GCGGCGGGGAAGCGGCACGG
Product: CheR-type MCP methyltransferase
Products: S-adenosyl-L-homocysteine; protein L-glutamate methyl ester; Tsrglu-Me; Trgglu-Me; Targlu-Me; Tapglu-Me + [C]
Alternate protein names: ORF19 [H]
Number of amino acids: Translated: 853; Mature: 853
Protein sequence:
>853_residues MLGPVARRGIDPRAKNAVIDQDHFPIVGIGASAGGIEAMRGFFRGVPERLEAAFVVVTHLGREHKSLLPDVLARLTPLKV EAAADGVPVGPGTVYVMPSGFVMGIAGGRLTLTPLGEGRETKPIDIFLTALALDQGERAVGVILSGGDGDGAIGIKAIKE HGGLTLAQTADGYGPETADMPLSSLRTGFVDFGETAERMGDRIAAHVAAAPIAPDAQADGVAREFEAELLTEIFAILRRQ VGHDFSGYKPSTFGRRLQRRIGVVGASGLDGYLKLLRADPAEVGVLFRDLLIGVTNFFRDTAAFEALTAQVIPKLFDARA ATDVIRIWVPACSTGEEVYSLAILLREHMVTLADPPRVQIFATDIDERALTVARTGRYPTAYLSAVSPERIGQHFVVEGD SAVVEKAVRDLCTFAPHNVLRDPPFSRLDLVSCRNLMIYLGVEAQQQLMPVLHYALRPRGYLFIGMAENVTRFEDLFETI DKQHRIFQARDAIPPARLRPMSGTDTPILANAGHPQRRNVTTHAALRHAVETQMLSEFTPPHAVVTRTGEAVHYSSRIGD HLEVVPGQPTRELAAMARKGLRLDLRTALREAIEGNILVVREGIGVELPDGRFQSISLVVKPLNTPGAVEPLFLVVFNEA AAPVERERQQALKANERDTALQGLERELSVTRERLQAVIEEYETSLEELKSSNEELYSVNEEMQAANEELEASKEETQSL NEELQTMNSELASKIEAVDQLTDDQATLFEGMNIASVLLTPDLHIRMFTNAAAQIFGLQPSDVGRSLRNFSAPIPLAWLV DEIPTVLAANRPSERTIEGADGARYRVRLMASSRKGGRRPGIVANFTNLLEQE
Sequences:
>Translated_853_residues MLGPVARRGIDPRAKNAVIDQDHFPIVGIGASAGGIEAMRGFFRGVPERLEAAFVVVTHLGREHKSLLPDVLARLTPLKV EAAADGVPVGPGTVYVMPSGFVMGIAGGRLTLTPLGEGRETKPIDIFLTALALDQGERAVGVILSGGDGDGAIGIKAIKE HGGLTLAQTADGYGPETADMPLSSLRTGFVDFGETAERMGDRIAAHVAAAPIAPDAQADGVAREFEAELLTEIFAILRRQ VGHDFSGYKPSTFGRRLQRRIGVVGASGLDGYLKLLRADPAEVGVLFRDLLIGVTNFFRDTAAFEALTAQVIPKLFDARA ATDVIRIWVPACSTGEEVYSLAILLREHMVTLADPPRVQIFATDIDERALTVARTGRYPTAYLSAVSPERIGQHFVVEGD SAVVEKAVRDLCTFAPHNVLRDPPFSRLDLVSCRNLMIYLGVEAQQQLMPVLHYALRPRGYLFIGMAENVTRFEDLFETI DKQHRIFQARDAIPPARLRPMSGTDTPILANAGHPQRRNVTTHAALRHAVETQMLSEFTPPHAVVTRTGEAVHYSSRIGD HLEVVPGQPTRELAAMARKGLRLDLRTALREAIEGNILVVREGIGVELPDGRFQSISLVVKPLNTPGAVEPLFLVVFNEA AAPVERERQQALKANERDTALQGLERELSVTRERLQAVIEEYETSLEELKSSNEELYSVNEEMQAANEELEASKEETQSL NEELQTMNSELASKIEAVDQLTDDQATLFEGMNIASVLLTPDLHIRMFTNAAAQIFGLQPSDVGRSLRNFSAPIPLAWLV DEIPTVLAANRPSERTIEGADGARYRVRLMASSRKGGRRPGIVANFTNLLEQE >Mature_853_residues MLGPVARRGIDPRAKNAVIDQDHFPIVGIGASAGGIEAMRGFFRGVPERLEAAFVVVTHLGREHKSLLPDVLARLTPLKV EAAADGVPVGPGTVYVMPSGFVMGIAGGRLTLTPLGEGRETKPIDIFLTALALDQGERAVGVILSGGDGDGAIGIKAIKE HGGLTLAQTADGYGPETADMPLSSLRTGFVDFGETAERMGDRIAAHVAAAPIAPDAQADGVAREFEAELLTEIFAILRRQ VGHDFSGYKPSTFGRRLQRRIGVVGASGLDGYLKLLRADPAEVGVLFRDLLIGVTNFFRDTAAFEALTAQVIPKLFDARA ATDVIRIWVPACSTGEEVYSLAILLREHMVTLADPPRVQIFATDIDERALTVARTGRYPTAYLSAVSPERIGQHFVVEGD SAVVEKAVRDLCTFAPHNVLRDPPFSRLDLVSCRNLMIYLGVEAQQQLMPVLHYALRPRGYLFIGMAENVTRFEDLFETI DKQHRIFQARDAIPPARLRPMSGTDTPILANAGHPQRRNVTTHAALRHAVETQMLSEFTPPHAVVTRTGEAVHYSSRIGD HLEVVPGQPTRELAAMARKGLRLDLRTALREAIEGNILVVREGIGVELPDGRFQSISLVVKPLNTPGAVEPLFLVVFNEA AAPVERERQQALKANERDTALQGLERELSVTRERLQAVIEEYETSLEELKSSNEELYSVNEEMQAANEELEASKEETQSL NEELQTMNSELASKIEAVDQLTDDQATLFEGMNIASVLLTPDLHIRMFTNAAAQIFGLQPSDVGRSLRNFSAPIPLAWLV DEIPTVLAANRPSERTIEGADGARYRVRLMASSRKGGRRPGIVANFTNLLEQE
Specific function: Methylation Of The Membrane-Bound Methyl-Accepting Chemotaxis Proteins (Mcp) To Form Gamma-Glutamyl Methyl Ester Residues In Mcp. [C]
COG id: COG1352
COG function: function code NT; Methylase of chemotaxis methyl-accepting proteins
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 cheR-type methyltransferase domain [H]
Homologues:
Organism=Escherichia coli, GI1788193, Length=258, Percent_Identity=31.3953488372093, Blast_Score=102, Evalue=1e-22, Organism=Escherichia coli, GI1788192, Length=189, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=1e-12,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR022642 - InterPro: IPR000780 - InterPro: IPR022641 - InterPro: IPR000014 - InterPro: IPR013656 - InterPro: IPR000673 [H]
Pfam domain/function: PF01339 CheB_methylest; PF01739 CheR; PF03705 CheR_N; PF08448 PAS_4 [H]
EC number: 2.1.1.80 [C]
Molecular weight: Translated: 92970; Mature: 92970
Theoretical pI: Translated: 5.22; Mature: 5.22
Prosite motif: PS00092 N6_MTASE ; PS50123 CHER ; PS50122 CHEB
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 2.1 %Met (Translated Protein) 2.5 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 2.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLGPVARRGIDPRAKNAVIDQDHFPIVGIGASAGGIEAMRGFFRGVPERLEAAFVVVTHL CCCCHHHCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH GREHKSLLPDVLARLTPLKVEAAADGVPVGPGTVYVMPSGFVMGIAGGRLTLTPLGEGRE CCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEECCCEEEEECCCEEEEEECCCCCC TKPIDIFLTALALDQGERAVGVILSGGDGDGAIGIKAIKEHGGLTLAQTADGYGPETADM CCCHHHEEEEHHHCCCCCEEEEEEECCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCC PLSSLRTGFVDFGETAERMGDRIAAHVAAAPIAPDAQADGVAREFEAELLTEIFAILRRQ CHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH VGHDFSGYKPSTFGRRLQRRIGVVGASGLDGYLKLLRADPAEVGVLFRDLLIGVTNFFRD HCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH TAAFEALTAQVIPKLFDARAATDVIRIWVPACSTGEEVYSLAILLREHMVTLADPPRVQI HHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEE FATDIDERALTVARTGRYPTAYLSAVSPERIGQHFVVEGDSAVVEKAVRDLCTFAPHNVL EEECCCCHHEEHHHCCCCCHHHHHCCCHHHCCCEEEEECCHHHHHHHHHHHHHHCCCHHH RDPPFSRLDLVSCRNLMIYLGVEAQQQLMPVLHYALRPRGYLFIGMAENVTRFEDLFETI CCCCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH DKQHRIFQARDAIPPARLRPMSGTDTPILANAGHPQRRNVTTHAALRHAVETQMLSEFTP HHHHHHHHHHCCCCHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCC PHAVVTRTGEAVHYSSRIGDHLEVVPGQPTRELAAMARKGLRLDLRTALREAIEGNILVV CCEEEECCCCCEEEHHHCCCCEEECCCCCHHHHHHHHHCCCEEHHHHHHHHHHCCCEEEE REGIGVELPDGRFQSISLVVKPLNTPGAVEPLFLVVFNEAAAPVERERQQALKANERDTA ECCCCEECCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHH LQGLERELSVTRERLQAVIEEYETSLEELKSSNEELYSVNEEMQAANEELEASKEETQSL HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH NEELQTMNSELASKIEAVDQLTDDQATLFEGMNIASVLLTPDLHIRMFTNAAAQIFGLQP HHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEECCCCEEEEECCHHHHHCCCCH SDVGRSLRNFSAPIPLAWLVDEIPTVLAANRPSERTIEGADGARYRVRLMASSRKGGRRP HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCC GIVANFTNLLEQE CHHHHHHHHHCCC >Mature Secondary Structure MLGPVARRGIDPRAKNAVIDQDHFPIVGIGASAGGIEAMRGFFRGVPERLEAAFVVVTHL CCCCHHHCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH GREHKSLLPDVLARLTPLKVEAAADGVPVGPGTVYVMPSGFVMGIAGGRLTLTPLGEGRE CCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEECCCEEEEECCCEEEEEECCCCCC TKPIDIFLTALALDQGERAVGVILSGGDGDGAIGIKAIKEHGGLTLAQTADGYGPETADM CCCHHHEEEEHHHCCCCCEEEEEEECCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCC PLSSLRTGFVDFGETAERMGDRIAAHVAAAPIAPDAQADGVAREFEAELLTEIFAILRRQ CHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH VGHDFSGYKPSTFGRRLQRRIGVVGASGLDGYLKLLRADPAEVGVLFRDLLIGVTNFFRD HCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH TAAFEALTAQVIPKLFDARAATDVIRIWVPACSTGEEVYSLAILLREHMVTLADPPRVQI HHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEE FATDIDERALTVARTGRYPTAYLSAVSPERIGQHFVVEGDSAVVEKAVRDLCTFAPHNVL EEECCCCHHEEHHHCCCCCHHHHHCCCHHHCCCEEEEECCHHHHHHHHHHHHHHCCCHHH RDPPFSRLDLVSCRNLMIYLGVEAQQQLMPVLHYALRPRGYLFIGMAENVTRFEDLFETI CCCCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH DKQHRIFQARDAIPPARLRPMSGTDTPILANAGHPQRRNVTTHAALRHAVETQMLSEFTP HHHHHHHHHHCCCCHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCC PHAVVTRTGEAVHYSSRIGDHLEVVPGQPTRELAAMARKGLRLDLRTALREAIEGNILVV CCEEEECCCCCEEEHHHCCCCEEECCCCCHHHHHHHHHCCCEEHHHHHHHHHHCCCEEEE REGIGVELPDGRFQSISLVVKPLNTPGAVEPLFLVVFNEAAAPVERERQQALKANERDTA ECCCCEECCCCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHH LQGLERELSVTRERLQAVIEEYETSLEELKSSNEELYSVNEEMQAANEELEASKEETQSL HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH NEELQTMNSELASKIEAVDQLTDDQATLFEGMNIASVLLTPDLHIRMFTNAAAQIFGLQP HHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEECCCCEEEEECCHHHHHCCCCH SDVGRSLRNFSAPIPLAWLVDEIPTVLAANRPSERTIEGADGARYRVRLMASSRKGGRRP HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCC GIVANFTNLLEQE CHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: Ca2+; Mg2+ [C]
Kcat value (1/min): 10 [C]
Specific activity: NA
Km value (mM): 0.017 {S-adenosyl-L-methionine}} 0.01 {S-adenosyl-L-methionine}} [C]
Substrates: S-Adenosyl-L-methionine; protein L-glutamate; Tsrglu; Trgglu; Targlu; Tapglu [C]
Specific reaction: S-Adenosyl-L-methionine + protein L-glutamate =S-adenosyl-L-homocysteine + protein L-glutamate methyl ester S-adenosyl-L-methionine + Tsrglu = S-adenosyl-homocysteine + Tsrglu-Me S-adenosyl-L-methionine + Trgglu = S-adenosyl-homocysteine + Trgglu-Me S-ade
General reaction: Methyl group transfer [C]
Inhibitor: A9145C; Ca2+; Methylated receptor protein; S-Adenosyl -L-homocysteine; Sinefungin [C]
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8497190 [H]