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The map label for this gene is gltX2

Identifier: 170751931

GI number: 170751931

Start: 5882028

End: 5883368

Strand: Direct

Name: gltX2

Synonym: Mrad2831_5563

Alternate gene names: 170751931

Gene position: 5882028-5883368 (Clockwise)

Preceding gene: 170751929

Following gene: 170751935

Centisome position: 96.78

GC content: 75.54

Gene sequence:

>1341_bases
ATGACTCTCGTCCGCTTCGCACCCTCGCCGACCGGGTATCTCCATATCGGCAACGCCCGGCCGGCCCTGCTCAACGCCCT
GTTCGCCCGCCGGACCGGCGGCCGCTTCCTGCTCCGCCTCGACGATACCGACGCGGAGCGCTCCACCGAGGCCTTCGCCG
AAGCGATCGGCGAGGATCTCGCGTGGCTCGGCATCGTGCCCGACCTGTTCGCCCGGCAGAGCGCCCGCACGGCGCAGCAC
GACGCCGCCGCCGACCGCCTGCGCGCAGCGGGCCGGCTCTACCCCTGCTACGAGACCCCGGAGGAGCTGGAGCGCCGCCG
CAGGCGCCAGCTCGGGCGCGGCCAACCGCCGATCTACGACCGGGCGGCGCTGCGGCTCACCGGCGACGAGCGGGCGGCGC
TCGAGGCCGAGGGCCGCCGGCCGCACTGGCGGTTCCTGCTGGAGGCGCGGACCGTCGGCTGGGACGATCTCGTCCGCGGC
CCGGCCCACGTCGACTGCGCGTCCCTGTCGGATCCGGTGCTGATCCGCGCCGACGGCAGCTACCTCTACACGCTGCCGTC
GGTGGTGGACGACGCCGATCTCGGCATCACCCACGTGATCCGCGGCGAGGACCACGTCACGAACACCGGCGTGCAGGTGC
AGATCTTCGAGGCCCTGGGCGCGGCCGTTCCGGTCTTCGGCCACCACAACCTGCTGACGACCGCCGACGGGGAGGGCCTG
TCGAAGCGCCTCGGCCACCTGTCGCTGCGCGGCCTGCGCGAGGCCGGCTACGAGCCCGCGGCGGTGCGCTCGCTGGCGGT
GCTGACCGGCTCGGCCGAATCGGTCCGGGCGGTGCCCGACCTCGACACCCTCGCGGGCCTCGTCGATCTCGGGGAGATCT
CGCGGGCGCCGGCCCGGTTCGACCCGGCGGAGCTCGACGGCCTCAACGCCCGCCTGATCCACGCCATGCCGTACCGGGAG
GCGGCCGCGCGCCTCGCGGATCTCGGGATCCCTGCGGACCGGGCCGAGGCCTTCTGGCTCGCGGTGCGCGCCAACCTGAG
CCGGGTTCCGGAGGCGGCGCCGTGGTGGCGGGTCGTGACCGGTCCGGTCGAGCCGGTGCTCACCGAGCCCGCCGTGATCG
CGGCCGCCGTCGAGAGCCTGCCCCCCGAGCCGTTCGGCCCGGAGACCTGGAACGCGTGGACGACCGAGATCAGGACTCGC
ACCGGCGCCAAGGGCCGCGGCCTGTTCATGCCGCTCCGGCTCGCGCTGACCGGCCTCGAGCACGGGCCGGACCTCGCCGG
CCTGCTGCCGCTGATCGGGCGGGAGCGGGCGGCGCGCCGGCTCTCCGGCGCGGCCGCCTGA

Upstream 100 bases:

>100_bases
CCGCCTCCTGGGTAGCGGGCCGCCGCGGGTCGGCGCATCGGGCCGGTGTGATCGTGAAGTCCCGCGCACATTCCCGCGGA
GATCGGCTAGAGAGCGGGCC

Downstream 100 bases:

>100_bases
GCGACCCGGCCCGGACAGCCCCGCCGGCGTTCGTAATCCTAGGCCGTTCGGCGGCGGTTCAGCGGCCGGGTCGCGGGCGC
GCCTCGATCAGCGCGGCATC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTLVRFAPSPTGYLHIGNARPALLNALFARRTGGRFLLRLDDTDAERSTEAFAEAIGEDLAWLGIVPDLFARQSARTAQH
DAAADRLRAAGRLYPCYETPEELERRRRRQLGRGQPPIYDRAALRLTGDERAALEAEGRRPHWRFLLEARTVGWDDLVRG
PAHVDCASLSDPVLIRADGSYLYTLPSVVDDADLGITHVIRGEDHVTNTGVQVQIFEALGAAVPVFGHHNLLTTADGEGL
SKRLGHLSLRGLREAGYEPAAVRSLAVLTGSAESVRAVPDLDTLAGLVDLGEISRAPARFDPAELDGLNARLIHAMPYRE
AAARLADLGIPADRAEAFWLAVRANLSRVPEAAPWWRVVTGPVEPVLTEPAVIAAAVESLPPEPFGPETWNAWTTEIRTR
TGAKGRGLFMPLRLALTGLEHGPDLAGLLPLIGRERAARRLSGAAA

Sequences:

>Translated_446_residues
MTLVRFAPSPTGYLHIGNARPALLNALFARRTGGRFLLRLDDTDAERSTEAFAEAIGEDLAWLGIVPDLFARQSARTAQH
DAAADRLRAAGRLYPCYETPEELERRRRRQLGRGQPPIYDRAALRLTGDERAALEAEGRRPHWRFLLEARTVGWDDLVRG
PAHVDCASLSDPVLIRADGSYLYTLPSVVDDADLGITHVIRGEDHVTNTGVQVQIFEALGAAVPVFGHHNLLTTADGEGL
SKRLGHLSLRGLREAGYEPAAVRSLAVLTGSAESVRAVPDLDTLAGLVDLGEISRAPARFDPAELDGLNARLIHAMPYRE
AAARLADLGIPADRAEAFWLAVRANLSRVPEAAPWWRVVTGPVEPVLTEPAVIAAAVESLPPEPFGPETWNAWTTEIRTR
TGAKGRGLFMPLRLALTGLEHGPDLAGLLPLIGRERAARRLSGAAA
>Mature_445_residues
TLVRFAPSPTGYLHIGNARPALLNALFARRTGGRFLLRLDDTDAERSTEAFAEAIGEDLAWLGIVPDLFARQSARTAQHD
AAADRLRAAGRLYPCYETPEELERRRRRQLGRGQPPIYDRAALRLTGDERAALEAEGRRPHWRFLLEARTVGWDDLVRGP
AHVDCASLSDPVLIRADGSYLYTLPSVVDDADLGITHVIRGEDHVTNTGVQVQIFEALGAAVPVFGHHNLLTTADGEGLS
KRLGHLSLRGLREAGYEPAAVRSLAVLTGSAESVRAVPDLDTLAGLVDLGEISRAPARFDPAELDGLNARLIHAMPYREA
AARLADLGIPADRAEAFWLAVRANLSRVPEAAPWWRVVTGPVEPVLTEPAVIAAAVESLPPEPFGPETWNAWTTEIRTRT
GAKGRGLFMPLRLALTGLEHGPDLAGLLPLIGRERAARRLSGAAA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=487, Percent_Identity=29.9794661190965, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI62241042, Length=258, Percent_Identity=28.6821705426357, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788743, Length=473, Percent_Identity=32.76955602537, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI226510925, Length=242, Percent_Identity=33.0578512396694, Blast_Score=88, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI71993394, Length=468, Percent_Identity=28.2051282051282, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17511007, Length=108, Percent_Identity=35.1851851851852, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324540, Length=481, Percent_Identity=28.4823284823285, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321192, Length=217, Percent_Identity=29.4930875576037, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324742, Length=103, Percent_Identity=34.9514563106796, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24665321, Length=479, Percent_Identity=31.1064718162839, Blast_Score=192, Evalue=3e-49,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_METRJ (B1M0I6)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001758191.1
- ProteinModelPortal:   B1M0I6
- SMR:   B1M0I6
- GeneID:   6141637
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_5563
- HOGENOM:   HBG628189
- OMA:   PHWRFKL
- ProtClustDB:   PRK12558
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 48358; Mature: 48227

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 242-242

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLVRFAPSPTGYLHIGNARPALLNALFARRTGGRFLLRLDDTDAERSTEAFAEAIGEDL
CEEEEECCCCCCEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCH
AWLGIVPDLFARQSARTAQHDAAADRLRAAGRLYPCYETPEELERRRRRQLGRGQPPIYD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
RAALRLTGDERAALEAEGRRPHWRFLLEARTVGWDDLVRGPAHVDCASLSDPVLIRADGS
CEEEEECCCCCCEECCCCCCCCEEEEEECCCCCHHHHHCCCCCEECCCCCCCEEEEECCC
YLYTLPSVVDDADLGITHVIRGEDHVTNTGVQVQIFEALGAAVPVFGHHNLLTTADGEGL
EEEECCCHHCCCCCCEEEEECCCCCEECCCCEEEEHHHHCCCCCCCCCCCEEEECCCCCH
SKRLGHLSLRGLREAGYEPAAVRSLAVLTGSAESVRAVPDLDTLAGLVDLGEISRAPARF
HHHHHHHHHHHHHHCCCCHHHHHHHHHEECCHHHHCCCCCHHHHHHHHHHHHHCCCCCCC
DPAELDGLNARLIHAMPYREAAARLADLGIPADRAEAFWLAVRANLSRVPEAAPWWRVVT
CCHHCCCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHEEEEEEHHHHHCCCCCCCCEEEC
GPVEPVLTEPAVIAAAVESLPPEPFGPETWNAWTTEIRTRTGAKGRGLFMPLRLALTGLE
CCCCCHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEHHHHHHHHCCC
HGPDLAGLLPLIGRERAARRLSGAAA
CCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TLVRFAPSPTGYLHIGNARPALLNALFARRTGGRFLLRLDDTDAERSTEAFAEAIGEDL
EEEEECCCCCCEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCH
AWLGIVPDLFARQSARTAQHDAAADRLRAAGRLYPCYETPEELERRRRRQLGRGQPPIYD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
RAALRLTGDERAALEAEGRRPHWRFLLEARTVGWDDLVRGPAHVDCASLSDPVLIRADGS
CEEEEECCCCCCEECCCCCCCCEEEEEECCCCCHHHHHCCCCCEECCCCCCCEEEEECCC
YLYTLPSVVDDADLGITHVIRGEDHVTNTGVQVQIFEALGAAVPVFGHHNLLTTADGEGL
EEEECCCHHCCCCCCEEEEECCCCCEECCCCEEEEHHHHCCCCCCCCCCCEEEECCCCCH
SKRLGHLSLRGLREAGYEPAAVRSLAVLTGSAESVRAVPDLDTLAGLVDLGEISRAPARF
HHHHHHHHHHHHHHCCCCHHHHHHHHHEECCHHHHCCCCCHHHHHHHHHHHHHCCCCCCC
DPAELDGLNARLIHAMPYREAAARLADLGIPADRAEAFWLAVRANLSRVPEAAPWWRVVT
CCHHCCCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHEEEEEEHHHHHCCCCCCCCEEEC
GPVEPVLTEPAVIAAAVESLPPEPFGPETWNAWTTEIRTRTGAKGRGLFMPLRLALTGLE
CCCCCHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEHHHHHHHHCCC
HGPDLAGLLPLIGRERAARRLSGAAA
CCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA