Click here to switch to the map view.

The map label for this gene is splB [H]

Identifier: 170751806

GI number: 170751806

Start: 5744296

End: 5745363

Strand: Direct

Name: splB [H]

Synonym: Mrad2831_5436

Alternate gene names: 170751806

Gene position: 5744296-5745363 (Clockwise)

Preceding gene: 170751804

Following gene: 170751807

Centisome position: 94.51

GC content: 69.66

Gene sequence:

>1068_bases
ATGGCCCTGACCCGCGACCTGCTCGACATCCGGACGATCTATCACGAGCCCGCCGTGGGCGACTTCCCGCTGGGCCGGGA
GATCCTGGCGCGCTTTCCCGACGCCGCGCGCATCGTCGTGCCGTCGCACTGGAACATTCCGGAACTCCACGGCAACGCGG
GCTCGGTGGAGGATTGGGTGAGGATCAAGCGCTCGACCCTCGTCCTCGGCGTGAAGAAGGGCCTCACGATGCGCCCGAAC
GGCCGCAGCGCCCACTTCATCGCGCCCTCGACCTCCAACGGCTGCGCCATGGCCTGCGCCTACTGCTACGTGCCGCGCCG
CAAGGGCTACTCCAACCCGATCTCGCTGTTCGTCAACGTCGAGCAGGCCTGCGCGGCGATCGCCCGCCACGCCGGGCGCC
AGGGGCGCCTCCCGGAGCCGGATCCGATCGATCCGGAGTACTGGGTCTACGATATCGGCGAGAACGGTGACCTGTCGGTG
GACGCGGCGGTGAGCGACGGCGCGCGCGCCCTGGTCGCGCTGTTCCGCACCCTGCCCAACGCCAAGGCCTCCTTCGCCAC
GAAGGCCGTGAACCGCGACCTCCTGGCCTACGATCCGCAGGGCAAGACGCGCATCCGCTTCTCGCTCATGCCGGCGCGGA
TCGCCCGGATCGTCGACGTGCGCACCGCCCCGATTCCCGAGCGGATCGCCGCCATCGACGACTTCGTGGCCGCCGGCTAC
GAGGTCCACGCCAACTTCTCGCCCGTGATCCTCTACGAGGGCTGGGAGGCGGACTGGCGGGCGCTGTTCGCCGAGATCGA
CGCGACGCTGTCCGACGCCGCCAAGGCGCAGCTCAAGTGCGAGATCATCATGCTGACCCACAACGCGGATCTGCATGCGG
TCAATCTCGGCTGGCACCCGAGGGCCGAGGACCTGCTCTGGCGGCCGGACATCCAGGAGGCGAAGGTCTCGGAGGGCGGC
GGCGCCAATCTGCGCTACCGGGCCGGCTGGAAGGGCCGGTGGCTCGCGCGGTTCAAGGCCCTGCTCGCCGAGACGATGCC
CTACTGCACGGTGCGCTACGCGTTCTGA

Upstream 100 bases:

>100_bases
CGCGCGCCGCGGGGACAGAGACGACCGGTCGCAAGGCATTCCGGCCGCGTCGGCGCGCACCATATCGGGTCCCGGGCCGC
TTCCGGGCCCCGGATCGTCC

Downstream 100 bases:

>100_bases
GCGCGGATGGCTGAACTTCCGGGCATGCGCGGCGTTGGCGCGCCAGGGGAGGAAAGCTCAGCCATGTCGACGCACGAGGA
CCATATCCGCGAGGCGGTCG

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MALTRDLLDIRTIYHEPAVGDFPLGREILARFPDAARIVVPSHWNIPELHGNAGSVEDWVRIKRSTLVLGVKKGLTMRPN
GRSAHFIAPSTSNGCAMACAYCYVPRRKGYSNPISLFVNVEQACAAIARHAGRQGRLPEPDPIDPEYWVYDIGENGDLSV
DAAVSDGARALVALFRTLPNAKASFATKAVNRDLLAYDPQGKTRIRFSLMPARIARIVDVRTAPIPERIAAIDDFVAAGY
EVHANFSPVILYEGWEADWRALFAEIDATLSDAAKAQLKCEIIMLTHNADLHAVNLGWHPRAEDLLWRPDIQEAKVSEGG
GANLRYRAGWKGRWLARFKALLAETMPYCTVRYAF

Sequences:

>Translated_355_residues
MALTRDLLDIRTIYHEPAVGDFPLGREILARFPDAARIVVPSHWNIPELHGNAGSVEDWVRIKRSTLVLGVKKGLTMRPN
GRSAHFIAPSTSNGCAMACAYCYVPRRKGYSNPISLFVNVEQACAAIARHAGRQGRLPEPDPIDPEYWVYDIGENGDLSV
DAAVSDGARALVALFRTLPNAKASFATKAVNRDLLAYDPQGKTRIRFSLMPARIARIVDVRTAPIPERIAAIDDFVAAGY
EVHANFSPVILYEGWEADWRALFAEIDATLSDAAKAQLKCEIIMLTHNADLHAVNLGWHPRAEDLLWRPDIQEAKVSEGG
GANLRYRAGWKGRWLARFKALLAETMPYCTVRYAF
>Mature_354_residues
ALTRDLLDIRTIYHEPAVGDFPLGREILARFPDAARIVVPSHWNIPELHGNAGSVEDWVRIKRSTLVLGVKKGLTMRPNG
RSAHFIAPSTSNGCAMACAYCYVPRRKGYSNPISLFVNVEQACAAIARHAGRQGRLPEPDPIDPEYWVYDIGENGDLSVD
AAVSDGARALVALFRTLPNAKASFATKAVNRDLLAYDPQGKTRIRFSLMPARIARIVDVRTAPIPERIAAIDDFVAAGYE
VHANFSPVILYEGWEADWRALFAEIDATLSDAAKAQLKCEIIMLTHNADLHAVNLGWHPRAEDLLWRPDIQEAKVSEGGG
ANLRYRAGWKGRWLARFKALLAETMPYCTVRYAF

Specific function: Involved in repair of UV radiation-induced DNA damage during spore germination. Can repair thymine dimer 5-thyminyl-5,6- dihydrothymine (known as spore photoproduct (SP)) by in situ monomerization of SP to two thymines [H]

COG id: COG1533

COG function: function code L; DNA repair photolyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. SPL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR004594 [H]

Pfam domain/function: NA

EC number: =4.1.99.14 [H]

Molecular weight: Translated: 39390; Mature: 39259

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTRDLLDIRTIYHEPAVGDFPLGREILARFPDAARIVVPSHWNIPELHGNAGSVEDWV
CCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHH
RIKRSTLVLGVKKGLTMRPNGRSAHFIAPSTSNGCAMACAYCYVPRRKGYSNPISLFVNV
HHHHHEEEEEECCCCEECCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEH
EQACAAIARHAGRQGRLPEPDPIDPEYWVYDIGENGDLSVDAAVSDGARALVALFRTLPN
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCHHHHHHHHHHCCC
AKASFATKAVNRDLLAYDPQGKTRIRFSLMPARIARIVDVRTAPIPERIAAIDDFVAAGY
CHHHHHHHHHCCCEEEECCCCCEEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHCCE
EVHANFSPVILYEGWEADWRALFAEIDATLSDAAKAQLKCEIIMLTHNADLHAVNLGWHP
EEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCEEEEECCCCC
RAEDLLWRPDIQEAKVSEGGGANLRYRAGWKGRWLARFKALLAETMPYCTVRYAF
CCCCCEECCCCCHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure 
ALTRDLLDIRTIYHEPAVGDFPLGREILARFPDAARIVVPSHWNIPELHGNAGSVEDWV
CCHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHH
RIKRSTLVLGVKKGLTMRPNGRSAHFIAPSTSNGCAMACAYCYVPRRKGYSNPISLFVNV
HHHHHEEEEEECCCCEECCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEH
EQACAAIARHAGRQGRLPEPDPIDPEYWVYDIGENGDLSVDAAVSDGARALVALFRTLPN
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCHHHHHHHHHHCCC
AKASFATKAVNRDLLAYDPQGKTRIRFSLMPARIARIVDVRTAPIPERIAAIDDFVAAGY
CHHHHHHHHHCCCEEEECCCCCEEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHHHCCE
EVHANFSPVILYEGWEADWRALFAEIDATLSDAAKAQLKCEIIMLTHNADLHAVNLGWHP
EEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCEEEEECCCCC
RAEDLLWRPDIQEAKVSEGGGANLRYRAGWKGRWLARFKALLAETMPYCTVRYAF
CCCCCEECCCCCHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8449881; 9384377; 9353924 [H]