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The map label for this gene is nemA [H]

Identifier: 170751657

GI number: 170751657

Start: 5603092

End: 5604204

Strand: Direct

Name: nemA [H]

Synonym: Mrad2831_5285

Alternate gene names: 170751657

Gene position: 5603092-5604204 (Clockwise)

Preceding gene: 170751656

Following gene: 170751658

Centisome position: 92.19

GC content: 66.4

Gene sequence:

>1113_bases
ATGACGACGAGCCCGATCCTCCAACCCGTAACAATCGGCGATCTGCACCTCAAGAACCGCATCGTGATGGCGCCGCTGAC
CCGCAGCCGGTCTTCGACCGCGGGCGTACCGCCGGACTTCGCCGCCGATTATTACGGCCAGCGTGCCAGCGCCGGCCTCA
TTATCTCAGAGGCTACTAATATCTCGCAGCAGGCCGTGGGCTATGCCTACACGCCGGGCATCTGGTCGGACGAGCAGATC
GAGGCTTGGAAGCGCATCGTCTCGACGGTCCACGCCAACGACGGCTGCATCTTCCTCCAGCTGTGGCACACCGGCCGGAT
ATCCCATCCCGACCTGCAGCCAGGGGGCGGCCTCCCGGTCTCCGCTTCGGCGGTGAAGCCGGAGGGAACTGCCTTCACGA
TGGAGGGGATGAAGCCCCACGTCACCCCACGGGCGTTGGAGACCGACGAGATCCCCGGCATCGTCGAGGATTACCGGCGC
GCGGCCGTGAACGCCAAGCGCGCCGGCTTCGACGGGGTCGAGGTCCACTCGGCCAACAACTACCTGCTGGAGCAGTTCGT
GCGAGATTCGACCAACAAGCGAACCGATCGCTACGGTGGCTCGATCGAGAATCGTTTGCGGTTCCCGCTTGAGGTGGTCC
GTGCGGTGACCGAGGCCTGGGACGGCGGAAGCCGCGTCGGCATCCGGTTGTCGCCCGCCACGACGCAGCCCGGCGAGACA
CCGCTCGATTCCGAGACGCATGCCACCTTCGGCGCCTATGTCGATGCGCTCAACGAGTTCGGCTTGCTCTACATTCACAC
GATCGAGGGCGTCACGCAGCAGACCCGTGATGTGCCGGACGGCGTCGATTTCCTCGACCTGCGGCGCCGGTTCGGGGGCG
CCTATATCGGCAACAACCAGAACACCCTGGATCTCGCCGAGAAGGAACTGGCCGAGGGCCGCGCCGATCTGTTCAGCTTC
GGCCGGCCCTACCTTGCCAACCCGGACCTCGTCGGGCGGCTGGCGAGCGGGGCACCGCTGGCCGAGGCACCCAAGCAATA
CTGGTACGGCGGCGGTGCGACCGGTTACTCCGACTGGCCGGGCATGGACGGGCCGGTACCGGTACGGCGCTGA

Upstream 100 bases:

>100_bases
TTCACCCATGCCGAACGGTTGAACGCGGATCTGATCACCTTCCTGAAGGGCTGACCCGCGCTCCGCTCACCATTACCCCG
CACACACAATCCGAGGTACC

Downstream 100 bases:

>100_bases
ACCGATCACGGAGATCGGCACGTCGCCGATTGTGACCGAGGCGACGGCCTGTATCGTGTGAGTGTCTTGCTGCGCGCGGG
AGAGCGCTCGGCCAACCTCG

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTTSPILQPVTIGDLHLKNRIVMAPLTRSRSSTAGVPPDFAADYYGQRASAGLIISEATNISQQAVGYAYTPGIWSDEQI
EAWKRIVSTVHANDGCIFLQLWHTGRISHPDLQPGGGLPVSASAVKPEGTAFTMEGMKPHVTPRALETDEIPGIVEDYRR
AAVNAKRAGFDGVEVHSANNYLLEQFVRDSTNKRTDRYGGSIENRLRFPLEVVRAVTEAWDGGSRVGIRLSPATTQPGET
PLDSETHATFGAYVDALNEFGLLYIHTIEGVTQQTRDVPDGVDFLDLRRRFGGAYIGNNQNTLDLAEKELAEGRADLFSF
GRPYLANPDLVGRLASGAPLAEAPKQYWYGGGATGYSDWPGMDGPVPVRR

Sequences:

>Translated_370_residues
MTTSPILQPVTIGDLHLKNRIVMAPLTRSRSSTAGVPPDFAADYYGQRASAGLIISEATNISQQAVGYAYTPGIWSDEQI
EAWKRIVSTVHANDGCIFLQLWHTGRISHPDLQPGGGLPVSASAVKPEGTAFTMEGMKPHVTPRALETDEIPGIVEDYRR
AAVNAKRAGFDGVEVHSANNYLLEQFVRDSTNKRTDRYGGSIENRLRFPLEVVRAVTEAWDGGSRVGIRLSPATTQPGET
PLDSETHATFGAYVDALNEFGLLYIHTIEGVTQQTRDVPDGVDFLDLRRRFGGAYIGNNQNTLDLAEKELAEGRADLFSF
GRPYLANPDLVGRLASGAPLAEAPKQYWYGGGATGYSDWPGMDGPVPVRR
>Mature_369_residues
TTSPILQPVTIGDLHLKNRIVMAPLTRSRSSTAGVPPDFAADYYGQRASAGLIISEATNISQQAVGYAYTPGIWSDEQIE
AWKRIVSTVHANDGCIFLQLWHTGRISHPDLQPGGGLPVSASAVKPEGTAFTMEGMKPHVTPRALETDEIPGIVEDYRRA
AVNAKRAGFDGVEVHSANNYLLEQFVRDSTNKRTDRYGGSIENRLRFPLEVVRAVTEAWDGGSRVGIRLSPATTQPGETP
LDSETHATFGAYVDALNEFGLLYIHTIEGVTQQTRDVPDGVDFLDLRRRFGGAYIGNNQNTLDLAEKELAEGRADLFSFG
RPYLANPDLVGRLASGAPLAEAPKQYWYGGGATGYSDWPGMDGPVPVRR

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=375, Percent_Identity=47.7333333333333, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1789463, Length=353, Percent_Identity=29.7450424929178, Blast_Score=115, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17559802, Length=347, Percent_Identity=28.2420749279539, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559804, Length=239, Percent_Identity=30.1255230125523, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17564188, Length=238, Percent_Identity=30.672268907563, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI72001454, Length=213, Percent_Identity=30.9859154929577, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17565138, Length=266, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540738, Length=158, Percent_Identity=33.5443037974684, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=381, Percent_Identity=37.2703412073491, Blast_Score=203, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6321973, Length=374, Percent_Identity=36.6310160427808, Blast_Score=195, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40258; Mature: 40127

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSPILQPVTIGDLHLKNRIVMAPLTRSRSSTAGVPPDFAADYYGQRASAGLIISEATN
CCCCCCCCCEEECCEEECCEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEECCCC
ISQQAVGYAYTPGIWSDEQIEAWKRIVSTVHANDGCIFLQLWHTGRISHPDLQPGGGLPV
CHHHHCCEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCC
SASAVKPEGTAFTMEGMKPHVTPRALETDEIPGIVEDYRRAAVNAKRAGFDGVEVHSANN
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCH
YLLEQFVRDSTNKRTDRYGGSIENRLRFPLEVVRAVTEAWDGGSRVGIRLSPATTQPGET
HHHHHHHHHCCCCCHHHCCCCHHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
PLDSETHATFGAYVDALNEFGLLYIHTIEGVTQQTRDVPDGVDFLDLRRRFGGAYIGNNQ
CCCCCHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHCCEEECCCC
NTLDLAEKELAEGRADLFSFGRPYLANPDLVGRLASGAPLAEAPKQYWYGGGATGYSDWP
CHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHCCHHHCCCCCCCCCCCCC
GMDGPVPVRR
CCCCCCCCCC
>Mature Secondary Structure 
TTSPILQPVTIGDLHLKNRIVMAPLTRSRSSTAGVPPDFAADYYGQRASAGLIISEATN
CCCCCCCCEEECCEEECCEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEECCCC
ISQQAVGYAYTPGIWSDEQIEAWKRIVSTVHANDGCIFLQLWHTGRISHPDLQPGGGLPV
CHHHHCCEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCC
SASAVKPEGTAFTMEGMKPHVTPRALETDEIPGIVEDYRRAAVNAKRAGFDGVEVHSANN
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCH
YLLEQFVRDSTNKRTDRYGGSIENRLRFPLEVVRAVTEAWDGGSRVGIRLSPATTQPGET
HHHHHHHHHCCCCCHHHCCCCHHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
PLDSETHATFGAYVDALNEFGLLYIHTIEGVTQQTRDVPDGVDFLDLRRRFGGAYIGNNQ
CCCCCHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHCCEEECCCC
NTLDLAEKELAEGRADLFSFGRPYLANPDLVGRLASGAPLAEAPKQYWYGGGATGYSDWP
CHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHCCHHHCCCCCCCCCCCCC
GMDGPVPVRR
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]