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The map label for this gene is yngF [H]

Identifier: 170751623

GI number: 170751623

Start: 5571457

End: 5572278

Strand: Direct

Name: yngF [H]

Synonym: Mrad2831_5249

Alternate gene names: 170751623

Gene position: 5571457-5572278 (Clockwise)

Preceding gene: 170751622

Following gene: 170751628

Centisome position: 91.67

GC content: 62.29

Gene sequence:

>822_bases
GTGCGGTGCACGGTTCGCCACGAGTTCGCGGAGACGATCTTGCCGGACAATGCCAACCGGGACGGCTCGACACCGACCAT
TGTGGTCGATGGGCCCATTGCAACAATCACGCTTCGGCGCCCCATACACCATAACCGTCTTGAGGCAGCCGACTTCGCCG
TCTTGATGGCGGCGCTTGATCAGGTGGAGGCCAATCGGAACGTTCGCTGCCTCATCATCACAGCGACTGGTCCCACCTTC
AGCGCCGGTTACGACATCGGCAAGCTCGCGCTGGGCGGCGACAACGACGAGATGTTCGGCCTCTGCGACCGGGTGGAGAA
CCTCGTGCAGCCGACGATCTGTGCCTTGAACGGAAACGTCTACGGGGGAGCGACGGACCTCGCTTTGGCATGTGACATTC
GGCTGGGCATCGAGGGCATGAAGATGTTCATGCCGCCAGCGAAACTCGGGATGCATTACTACTACAGCGGGCTCCGCCGC
TACGTTCAACAGCTCGGCCTTGGGAACGCGAAGCGCCTCATCTTGAGCGGGGCGATGATCGAGGCCCCTGAAATGCTCCA
GATCGGCTTCCTGGACGAGTTACTCGCACCCGATGCCCTCCATGCCCGGGTAGTCGTGCTCGCCGGCATGATCGCGGATG
GGGCGCCTACCGCAGTCCAGGGCATGAAGGCATCCATGAACGCGATTGCCGCGGGCACTGCCGTAGCGCAGGAGGTTCAG
GACAGATACCTTTCCAGCCTGCGCTCGGACGACCTGAAGGAAGGACTACGGGCGCTCGCCGAGAAGCGAAAGCCGCGCTT
CACCGAGGCTGCGGATTTATAG

Upstream 100 bases:

>100_bases
GCCGGAAGCAGACATTCGTCGGCTGTTAGGTAATCGCGTCGAGCTTCCTGTCGCCGTGATAAGATTGTAGCCCTCGTGGT
CGAGCGTCAGCCGCAGGAAG

Downstream 100 bases:

>100_bases
CGAGCGCAAGCCACCGTCTGCAAAGGGTTGTGAGCGCTTAGAAGGCCAGGTCTGCTCGAACGGATTTGCCATCTGGATGC
GGACCAGGGGGCATCCACCC

Product: enoyl-CoA hydratase/isomerase

Products: crotonoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MRCTVRHEFAETILPDNANRDGSTPTIVVDGPIATITLRRPIHHNRLEAADFAVLMAALDQVEANRNVRCLIITATGPTF
SAGYDIGKLALGGDNDEMFGLCDRVENLVQPTICALNGNVYGGATDLALACDIRLGIEGMKMFMPPAKLGMHYYYSGLRR
YVQQLGLGNAKRLILSGAMIEAPEMLQIGFLDELLAPDALHARVVVLAGMIADGAPTAVQGMKASMNAIAAGTAVAQEVQ
DRYLSSLRSDDLKEGLRALAEKRKPRFTEAADL

Sequences:

>Translated_273_residues
MRCTVRHEFAETILPDNANRDGSTPTIVVDGPIATITLRRPIHHNRLEAADFAVLMAALDQVEANRNVRCLIITATGPTF
SAGYDIGKLALGGDNDEMFGLCDRVENLVQPTICALNGNVYGGATDLALACDIRLGIEGMKMFMPPAKLGMHYYYSGLRR
YVQQLGLGNAKRLILSGAMIEAPEMLQIGFLDELLAPDALHARVVVLAGMIADGAPTAVQGMKASMNAIAAGTAVAQEVQ
DRYLSSLRSDDLKEGLRALAEKRKPRFTEAADL
>Mature_273_residues
MRCTVRHEFAETILPDNANRDGSTPTIVVDGPIATITLRRPIHHNRLEAADFAVLMAALDQVEANRNVRCLIITATGPTF
SAGYDIGKLALGGDNDEMFGLCDRVENLVQPTICALNGNVYGGATDLALACDIRLGIEGMKMFMPPAKLGMHYYYSGLRR
YVQQLGLGNAKRLILSGAMIEAPEMLQIGFLDELLAPDALHARVVVLAGMIADGAPTAVQGMKASMNAIAAGTAVAQEVQ
DRYLSSLRSDDLKEGLRALAEKRKPRFTEAADL

Specific function: Unknown

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=283, Percent_Identity=25.4416961130742, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4502327, Length=251, Percent_Identity=28.2868525896414, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI87082183, Length=226, Percent_Identity=27.8761061946903, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787659, Length=243, Percent_Identity=28.8065843621399, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI221142681, Length=250, Percent_Identity=29.6, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1790281, Length=177, Percent_Identity=28.8135593220339, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=28.968253968254, Blast_Score=67, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=245, Percent_Identity=30.2040816326531, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=245, Percent_Identity=28.9795918367347, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=246, Percent_Identity=23.9837398373984, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17560910, Length=190, Percent_Identity=24.7368421052632, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=28.7449392712551, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=28.7449392712551, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=25.8964143426295, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.55

Molecular weight: Translated: 29413; Mature: 29413

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCTVRHEFAETILPDNANRDGSTPTIVVDGPIATITLRRPIHHNRLEAADFAVLMAALD
CCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
QVEANRNVRCLIITATGPTFSAGYDIGKLALGGDNDEMFGLCDRVENLVQPTICALNGNV
HHHCCCCEEEEEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCEEEEECCCE
YGGATDLALACDIRLGIEGMKMFMPPAKLGMHYYYSGLRRYVQQLGLGNAKRLILSGAMI
ECCCCCEEEEEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEECCCC
EAPEMLQIGFLDELLAPDALHARVVVLAGMIADGAPTAVQGMKASMNAIAAGTAVAQEVQ
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DRYLSSLRSDDLKEGLRALAEKRKPRFTEAADL
HHHHHHHCCHHHHHHHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure
MRCTVRHEFAETILPDNANRDGSTPTIVVDGPIATITLRRPIHHNRLEAADFAVLMAALD
CCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
QVEANRNVRCLIITATGPTFSAGYDIGKLALGGDNDEMFGLCDRVENLVQPTICALNGNV
HHHCCCCEEEEEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCEEEEECCCE
YGGATDLALACDIRLGIEGMKMFMPPAKLGMHYYYSGLRRYVQQLGLGNAKRLILSGAMI
ECCCCCEEEEEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEECCCC
EAPEMLQIGFLDELLAPDALHARVVVLAGMIADGAPTAVQGMKASMNAIAAGTAVAQEVQ
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DRYLSSLRSDDLKEGLRALAEKRKPRFTEAADL
HHHHHHHCCHHHHHHHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxybutanoyl-CoA

Specific reaction: (3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]