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The map label for this gene is cueO [H]

Identifier: 170751512

GI number: 170751512

Start: 5454057

End: 5455553

Strand: Reverse

Name: cueO [H]

Synonym: Mrad2831_5132

Alternate gene names: 170751512

Gene position: 5455553-5454057 (Counterclockwise)

Preceding gene: 170751518

Following gene: 170751510

Centisome position: 89.76

GC content: 74.01

Gene sequence:

>1497_bases
ATGCCGCCATCCCGCACCGTGCCGCCGACCGATTCGCGTGCCGGCACGCCGTCGCGCCGGACCCTGCTCGCCGCCGCGGC
GGCCTTCGGGCTCGCGCCGGAGGCCGTGCGGGCCCAGGCGCCGGCCGCCGCGCCAGGGGCGCCGGCGCCGTCCTCCGCGC
AGCCGGAGCCCGCGGCGGCGCCGGCGGCCCTGCCGGCGGCGCCCGGCAGGACGCGGTTCAGGCCGGAACCGGCGCCAGAG
ACGCCGGTCTGGTGCTTCGACGGCAAGGCCCTGCCGCCCGTCATCCGCGTGAAGCTCGGGCAGGCGGTACGCCTCAAGGT
GGAGAACCGGACCGACAGGGCGCTGTCGCTGCACTGGCACGGGGTGCGCAACGTCAACGCCATGGACGGCGTCGGCGGGG
TGACCCAGGCGCCGATCCCGCCGGGCGGCGACTTCCTCTACACTTTCACGCCGCCGGATGCCGGCTCCTACCTGATCCGG
CCCCTGGTCGTCGGCGGGGCGAGCGAGCCGTCGGGCCGCGGGCTCGCGGGCATGCTGGTCGTCGAGGAGACGAACCCGCC
CGCGGTCGACGCCGACGTCAGCGTCGTGCTGCAGGACTGGCGGCTCCAGGACGACGGCACCCTGATGCCGTTCGGGCAGA
CGGCGCTCGCCGCCGCGCACGGCCGCCTCGGCAACCTCGTGACCGTCAACGGCAAGCCGGTGCCGGGCGCCTTCGAGGCC
CGGCCGGGGTCCCGTATCCGCCTGCGCCTCGGCAATGCCTGCAACGCCCGGGCGACGCGCATCAAGTTCGAGGGCGTGAA
GGTCTACGTGGCGGCGGTCGACGGCCAGCCCACCGACACGTTCGAGCCCCTGCGGGCCACCCTCCCCTTCCCGCCCGGCA
CCCGCTACGACCTGCTCGTCGACCTGCCGGAGGAGGAAGGCGCCCGCTGCGAGATCCACGCGCTGATCGGCAAGGGCCTG
GTCCTGGCGAGCGTCGTCACCAAGGGTGCCCGGATCACCGAGAAGCGGCCCCCGATCGGGCCGATCGGCGAGAACAAGCG
CCTGCCGGCCGAGATCAAGCTCCAGAACGCCCTGCGCCGCGACGTGGTGATCACCGGCGGCGCCTTCGTGCCGAAGGGTA
AGGACGGGGCCAAGGACGGCCCCGCGCCCGACCCCGTCTACACGGGCGACCCGCAGAAGATCTGGTCGGTGAACGGCGCG
AGCGGCGCCGCCGGCACCGCGCCGATCTTCTCGGTGAAGCGCGGCCAAGTGGTGGTGCTGGCCCTGCGCAACGAGACCGC
CTTCCCGCAGGCCCTGCACCTGCACGGCCACTGCTTCCGCCTGCTCCATCCCCTGGACGACGGCTGGGAGCCCTACTGGC
TCGACACCTTTCAGCTGCTGGAGGGCCGCACAGCCCGGATCGGGTTCCTGGCGGACAATCCAGGGCGCTGGCTGATCAGC
GCCACGGTGCTGGAGCGGTTCGACACCGGCCTCTGGACGATGTTCGAGGTCACCTGA

Upstream 100 bases:

>100_bases
GCATGGGCCGGCGGGCCCGCAGCAAAAAACTTGCCCCTGTGGCGCGGCAGCCCGCGCGCCGCTAAGGAGCGGGCCCACCG
CGAGCCGCAGCCGAGATCCC

Downstream 100 bases:

>100_bases
GCGGTCAGCCGCCGGTCAACGCGACGAGGTCGCGCAGCACCGCCGGCATCAGCTCGGGGATGTCCTCGGCAATCAGGCCC
GGCCCGTGGCGCAGGCCGGC

Product: multicopper oxidase type 3

Products: NA

Alternate protein names: Copper efflux oxidase [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MPPSRTVPPTDSRAGTPSRRTLLAAAAAFGLAPEAVRAQAPAAAPGAPAPSSAQPEPAAAPAALPAAPGRTRFRPEPAPE
TPVWCFDGKALPPVIRVKLGQAVRLKVENRTDRALSLHWHGVRNVNAMDGVGGVTQAPIPPGGDFLYTFTPPDAGSYLIR
PLVVGGASEPSGRGLAGMLVVEETNPPAVDADVSVVLQDWRLQDDGTLMPFGQTALAAAHGRLGNLVTVNGKPVPGAFEA
RPGSRIRLRLGNACNARATRIKFEGVKVYVAAVDGQPTDTFEPLRATLPFPPGTRYDLLVDLPEEEGARCEIHALIGKGL
VLASVVTKGARITEKRPPIGPIGENKRLPAEIKLQNALRRDVVITGGAFVPKGKDGAKDGPAPDPVYTGDPQKIWSVNGA
SGAAGTAPIFSVKRGQVVVLALRNETAFPQALHLHGHCFRLLHPLDDGWEPYWLDTFQLLEGRTARIGFLADNPGRWLIS
ATVLERFDTGLWTMFEVT

Sequences:

>Translated_498_residues
MPPSRTVPPTDSRAGTPSRRTLLAAAAAFGLAPEAVRAQAPAAAPGAPAPSSAQPEPAAAPAALPAAPGRTRFRPEPAPE
TPVWCFDGKALPPVIRVKLGQAVRLKVENRTDRALSLHWHGVRNVNAMDGVGGVTQAPIPPGGDFLYTFTPPDAGSYLIR
PLVVGGASEPSGRGLAGMLVVEETNPPAVDADVSVVLQDWRLQDDGTLMPFGQTALAAAHGRLGNLVTVNGKPVPGAFEA
RPGSRIRLRLGNACNARATRIKFEGVKVYVAAVDGQPTDTFEPLRATLPFPPGTRYDLLVDLPEEEGARCEIHALIGKGL
VLASVVTKGARITEKRPPIGPIGENKRLPAEIKLQNALRRDVVITGGAFVPKGKDGAKDGPAPDPVYTGDPQKIWSVNGA
SGAAGTAPIFSVKRGQVVVLALRNETAFPQALHLHGHCFRLLHPLDDGWEPYWLDTFQLLEGRTARIGFLADNPGRWLIS
ATVLERFDTGLWTMFEVT
>Mature_497_residues
PPSRTVPPTDSRAGTPSRRTLLAAAAAFGLAPEAVRAQAPAAAPGAPAPSSAQPEPAAAPAALPAAPGRTRFRPEPAPET
PVWCFDGKALPPVIRVKLGQAVRLKVENRTDRALSLHWHGVRNVNAMDGVGGVTQAPIPPGGDFLYTFTPPDAGSYLIRP
LVVGGASEPSGRGLAGMLVVEETNPPAVDADVSVVLQDWRLQDDGTLMPFGQTALAAAHGRLGNLVTVNGKPVPGAFEAR
PGSRIRLRLGNACNARATRIKFEGVKVYVAAVDGQPTDTFEPLRATLPFPPGTRYDLLVDLPEEEGARCEIHALIGKGLV
LASVVTKGARITEKRPPIGPIGENKRLPAEIKLQNALRRDVVITGGAFVPKGKDGAKDGPAPDPVYTGDPQKIWSVNGAS
GAAGTAPIFSVKRGQVVVLALRNETAFPQALHLHGHCFRLLHPLDDGWEPYWLDTFQLLEGRTARIGFLADNPGRWLISA
TVLERFDTGLWTMFEVT

Specific function: Probably involved in periplasmic detoxification of copper by oxidizing Cu(+) to Cu(2+) and thus preventing its uptake into the cytoplasm. Possesses phenoloxidase and ferroxidase activities and might be involved in the production of polyphenolic compounds

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 plastocyanin-like domains [H]

Homologues:

Organism=Escherichia coli, GI1786314, Length=303, Percent_Identity=29.042904290429, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011706
- InterPro:   IPR011707
- InterPro:   IPR002355
- InterPro:   IPR008972
- InterPro:   IPR006311 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07731 Cu-oxidase_2; PF07732 Cu-oxidase_3 [H]

EC number: NA

Molecular weight: Translated: 52779; Mature: 52648

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPSRTVPPTDSRAGTPSRRTLLAAAAAFGLAPEAVRAQAPAAAPGAPAPSSAQPEPAAA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCC
PAALPAAPGRTRFRPEPAPETPVWCFDGKALPPVIRVKLGQAVRLKVENRTDRALSLHWH
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCEEEEEEECCCCEEEEEEEC
GVRNVNAMDGVGGVTQAPIPPGGDFLYTFTPPDAGSYLIRPLVVGGASEPSGRGLAGMLV
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCEEEEEE
VEETNPPAVDADVSVVLQDWRLQDDGTLMPFGQTALAAAHGRLGNLVTVNGKPVPGAFEA
EECCCCCCCCCCEEEEEEEEEECCCCEECCCCCHHHHHHCCCCCCEEEECCCCCCCCCCC
RPGSRIRLRLGNACNARATRIKFEGVKVYVAAVDGQPTDTFEPLRATLPFPPGTRYDLLV
CCCCEEEEEECCCCCCCEEEEEECCEEEEEEEECCCCCCCCCCHHEECCCCCCCCEEEEE
DLPEEEGARCEIHALIGKGLVLASVVTKGARITEKRPPIGPIGENKRLPAEIKLQNALRR
ECCCCCCCEEEEEEECCCCHHHHHHHHCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHCC
DVVITGGAFVPKGKDGAKDGPAPDPVYTGDPQKIWSVNGASGAAGTAPIFSVKRGQVVVL
CEEEECCEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEECCCCEEEE
ALRNETAFPQALHLHGHCFRLLHPLDDGWEPYWLDTFQLLEGRTARIGFLADNPGRWLIS
EECCCCCCCCEEECCHHHHHHHCCCCCCCCCEEHHHHHHHCCCEEEEEEEECCCCCEEEE
ATVLERFDTGLWTMFEVT
HHHHHHHCCCEEEEEEEC
>Mature Secondary Structure 
PPSRTVPPTDSRAGTPSRRTLLAAAAAFGLAPEAVRAQAPAAAPGAPAPSSAQPEPAAA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCC
PAALPAAPGRTRFRPEPAPETPVWCFDGKALPPVIRVKLGQAVRLKVENRTDRALSLHWH
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCEEEEEEECCCCEEEEEEEC
GVRNVNAMDGVGGVTQAPIPPGGDFLYTFTPPDAGSYLIRPLVVGGASEPSGRGLAGMLV
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCEEEEEE
VEETNPPAVDADVSVVLQDWRLQDDGTLMPFGQTALAAAHGRLGNLVTVNGKPVPGAFEA
EECCCCCCCCCCEEEEEEEEEECCCCEECCCCCHHHHHHCCCCCCEEEECCCCCCCCCCC
RPGSRIRLRLGNACNARATRIKFEGVKVYVAAVDGQPTDTFEPLRATLPFPPGTRYDLLV
CCCCEEEEEECCCCCCCEEEEEECCEEEEEEEECCCCCCCCCCHHEECCCCCCCCEEEEE
DLPEEEGARCEIHALIGKGLVLASVVTKGARITEKRPPIGPIGENKRLPAEIKLQNALRR
ECCCCCCCEEEEEEECCCCHHHHHHHHCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHCC
DVVITGGAFVPKGKDGAKDGPAPDPVYTGDPQKIWSVNGASGAAGTAPIFSVKRGQVVVL
CEEEECCEECCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEECCCCEEEE
ALRNETAFPQALHLHGHCFRLLHPLDDGWEPYWLDTFQLLEGRTARIGFLADNPGRWLIS
EECCCCCCCCEEECCHHHHHHHCCCCCCCCCEEHHHHHHHCCCEEEEEEEECCCCCEEEE
ATVLERFDTGLWTMFEVT
HHHHHHHCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]