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The map label for this gene is metB [H]

Identifier: 170751479

GI number: 170751479

Start: 5421334

End: 5422536

Strand: Reverse

Name: metB [H]

Synonym: Mrad2831_5099

Alternate gene names: 170751479

Gene position: 5422536-5421334 (Counterclockwise)

Preceding gene: 170751481

Following gene: 170751478

Centisome position: 89.22

GC content: 74.81

Gene sequence:

>1203_bases
ATGTCACAGCCTTACGCGCCGCAGGGTCGGTCGGATACCCGGAGCGACGCCGAGCCGGACTTCACGCGCCGGAGCCTCGC
GGCCCAGGCCATGGGCCGGATCGAGCCGGAGACGCGGGCGGTGGTCATGCCGCTCCACGTCGCCACCACCTTCATCCGCG
ATCCCGACAACGGCTACAGCTCCGGCTACAGCTACGCGCGGCCCGACAACGCCACGGTGCGTGAGGCGGAGTCGGTCCTG
GCGATGCTGGAGGGCGCCGGCGCCGGCGCGCTGCTGTTCGGCTCCGGGATGGCGGCGGCGACCGCGGTGTTCGGCGCGCT
GGAGCCCGGCGACCACGTGGTCGCCGGCACGGTCATGTACTGGGCGCTGAAGCGCTGGCTGCGCGAGGAGGCGCCCCGGC
GCGGCCTCACACTGACCTTCGCGGACACGGACGATCCGGACGCCCTGCGCGCCGCGATCGAGCCCGGCCGGACCAAGCTC
GTCTGGATCGAGACACCCGGCAACCCGCTCTGGACGGTCACCGACATCGCCGCGGCGGCCGAGATCGCCCACGCGGCGGG
GGCGCGGCTCGCCGTGGATTCCACGGCCGCCTCGCCGGTCCACACCCGGCCCCTGGAACTCGGCGCCGACATCGTGATGC
ACTCGGCCACCAAGATCCTCAACGGCCATTCCGACGTGGTGGCGGGCGTGCTGGCGGGCGCGCGGGCCGACGCGTTCTGG
GAGCGGATCCGGGGGATCCGCGCCGGCAGCGGCGGCATCCTCGGGCCCTTCGAGGCCTACCTGCTGATGCGCTCGCTCCG
GACCCTGCATCTCCGGGCGGCCGCGCAGGCGGCGGGCGCCCTGCGCCTCGCCGGGCGCCTGAGCGGACACCCCCACGTGA
CCCGCGTCCTCTACCCGGGTCTCCCGGACGATCCCGGCCACGCGGTCGCCTGCCGGCAGATGCGGGACGGGTTCGGCTTC
ATGCTGTCGATCCGGGTGGCCGGCGGCGAGGCCGGCGCCATCGCCACGGCGGCGCGGGTGCGGCTCTGGAAGCGGGCGAC
CTCGCTCGGCGGCGTCGAGAGCCTCATCGAGCACCGCGCCTCGGTGGAAGGCCCGGGCAGTCCCTGCCCGCCGGACCTGC
TGCGCCTGTCCACCGGCATCGAGGACACGGACGACCTGTACGGCGATCTCGATCAGGCCCTGCGCGGCGCCCACGCGGGG
TGA

Upstream 100 bases:

>100_bases
GTCATGATCGTCGGTCTCCACCGGGCGCGGCGTGTGGCGCGGCTTGCCGGGGGCCACGCGCCGTGGCACGCGCCCGTCTC
CATCCGCCGAGAATCGCCAG

Downstream 100 bases:

>100_bases
TCGTCGCGGGCGCTTGGTGCCGAACGGGTTCCTGGCGCGTTGTGCCGGCAGTTCCGGCGGCGCGAACCGCCACAGATCGC
GAGTCTGACGACATGACGAC

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSQPYAPQGRSDTRSDAEPDFTRRSLAAQAMGRIEPETRAVVMPLHVATTFIRDPDNGYSSGYSYARPDNATVREAESVL
AMLEGAGAGALLFGSGMAAATAVFGALEPGDHVVAGTVMYWALKRWLREEAPRRGLTLTFADTDDPDALRAAIEPGRTKL
VWIETPGNPLWTVTDIAAAAEIAHAAGARLAVDSTAASPVHTRPLELGADIVMHSATKILNGHSDVVAGVLAGARADAFW
ERIRGIRAGSGGILGPFEAYLLMRSLRTLHLRAAAQAAGALRLAGRLSGHPHVTRVLYPGLPDDPGHAVACRQMRDGFGF
MLSIRVAGGEAGAIATAARVRLWKRATSLGGVESLIEHRASVEGPGSPCPPDLLRLSTGIEDTDDLYGDLDQALRGAHAG

Sequences:

>Translated_400_residues
MSQPYAPQGRSDTRSDAEPDFTRRSLAAQAMGRIEPETRAVVMPLHVATTFIRDPDNGYSSGYSYARPDNATVREAESVL
AMLEGAGAGALLFGSGMAAATAVFGALEPGDHVVAGTVMYWALKRWLREEAPRRGLTLTFADTDDPDALRAAIEPGRTKL
VWIETPGNPLWTVTDIAAAAEIAHAAGARLAVDSTAASPVHTRPLELGADIVMHSATKILNGHSDVVAGVLAGARADAFW
ERIRGIRAGSGGILGPFEAYLLMRSLRTLHLRAAAQAAGALRLAGRLSGHPHVTRVLYPGLPDDPGHAVACRQMRDGFGF
MLSIRVAGGEAGAIATAARVRLWKRATSLGGVESLIEHRASVEGPGSPCPPDLLRLSTGIEDTDDLYGDLDQALRGAHAG
>Mature_399_residues
SQPYAPQGRSDTRSDAEPDFTRRSLAAQAMGRIEPETRAVVMPLHVATTFIRDPDNGYSSGYSYARPDNATVREAESVLA
MLEGAGAGALLFGSGMAAATAVFGALEPGDHVVAGTVMYWALKRWLREEAPRRGLTLTFADTDDPDALRAAIEPGRTKLV
WIETPGNPLWTVTDIAAAAEIAHAAGARLAVDSTAASPVHTRPLELGADIVMHSATKILNGHSDVVAGVLAGARADAFWE
RIRGIRAGSGGILGPFEAYLLMRSLRTLHLRAAAQAAGALRLAGRLSGHPHVTRVLYPGLPDDPGHAVACRQMRDGFGFM
LSIRVAGGEAGAIATAARVRLWKRATSLGGVESLIEHRASVEGPGSPCPPDLLRLSTGIEDTDDLYGDLDQALRGAHAG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=393, Percent_Identity=39.1857506361323, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI299473759, Length=393, Percent_Identity=36.6412213740458, Blast_Score=192, Evalue=6e-49,
Organism=Homo sapiens, GI25453487, Length=392, Percent_Identity=33.1632653061224, Blast_Score=160, Evalue=3e-39,
Organism=Escherichia coli, GI1790375, Length=379, Percent_Identity=39.8416886543536, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI1789383, Length=340, Percent_Identity=32.3529411764706, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71980708, Length=409, Percent_Identity=33.7408312958435, Blast_Score=215, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI17560056, Length=377, Percent_Identity=38.4615384615385, Blast_Score=214, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI17538055, Length=377, Percent_Identity=37.6657824933687, Blast_Score=209, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6319307, Length=375, Percent_Identity=39.7333333333333, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6321904, Length=370, Percent_Identity=30.5405405405405, Blast_Score=129, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6323333, Length=422, Percent_Identity=27.7251184834123, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321254, Length=388, Percent_Identity=27.0618556701031, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321170, Length=327, Percent_Identity=27.5229357798165, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922554, Length=373, Percent_Identity=38.3378016085791, Blast_Score=237, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 42170; Mature: 42039

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPYAPQGRSDTRSDAEPDFTRRSLAAQAMGRIEPETRAVVMPLHVATTFIRDPDNGYS
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCCCCCCC
SGYSYARPDNATVREAESVLAMLEGAGAGALLFGSGMAAATAVFGALEPGDHVVAGTVMY
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCCHHHHHHH
WALKRWLREEAPRRGLTLTFADTDDPDALRAAIEPGRTKLVWIETPGNPLWTVTDIAAAA
HHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCCCEEEHHHHHHHH
EIAHAAGARLAVDSTAASPVHTRPLELGADIVMHSATKILNGHSDVVAGVLAGARADAFW
HHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHH
ERIRGIRAGSGGILGPFEAYLLMRSLRTLHLRAAAQAAGALRLAGRLSGHPHVTRVLYPG
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
LPDDPGHAVACRQMRDGFGFMLSIRVAGGEAGAIATAARVRLWKRATSLGGVESLIEHRA
CCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SVEGPGSPCPPDLLRLSTGIEDTDDLYGDLDQALRGAHAG
CCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SQPYAPQGRSDTRSDAEPDFTRRSLAAQAMGRIEPETRAVVMPLHVATTFIRDPDNGYS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCCCCCCC
SGYSYARPDNATVREAESVLAMLEGAGAGALLFGSGMAAATAVFGALEPGDHVVAGTVMY
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCCHHHHHHH
WALKRWLREEAPRRGLTLTFADTDDPDALRAAIEPGRTKLVWIETPGNPLWTVTDIAAAA
HHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCCCEEEHHHHHHHH
EIAHAAGARLAVDSTAASPVHTRPLELGADIVMHSATKILNGHSDVVAGVLAGARADAFW
HHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHH
ERIRGIRAGSGGILGPFEAYLLMRSLRTLHLRAAAQAAGALRLAGRLSGHPHVTRVLYPG
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
LPDDPGHAVACRQMRDGFGFMLSIRVAGGEAGAIATAARVRLWKRATSLGGVESLIEHRA
CCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SVEGPGSPCPPDLLRLSTGIEDTDDLYGDLDQALRGAHAG
CCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]